Download: (right click - "save target as")

Pricelist
Download

Pricelist:

Nr.         Euro
1000 A. Vectors from the website       200,00
1001 Special vectors       250,00
1002 Vectors adapted to customers       on request
1003 RNAi or recombinase vectors       250,00
1004 Agrobacterium strain GV3101-pMP90RK       200,00
1005  Cas9 vectors       400,00
1006  Cas9 nickase amplification vector        100,00
           
  B. Cloning from plasmids        
2010 Cloning of a DNA fragment into a vector of your choice.       (sequencing not included) 250,00
2020 Cloning of a second DNA fragment into the same vector       (sequencing not included) 200,00
2021 Cloning of a third DNA fragment into the same vector       (sequencing not included) 175,00
2030 or cloning of the same DNA fragment into another vector      (sequencing not included) 200,00
2040 or cloning of a DNA fragment into a predigested vector      (sequencing not included) 200,00
2050 Cloning of a DNA fragment into a vector with the same selection marker    (sequencing not included) 300,00
2051 Cloning of a second DNA fragment into a vector with the same selection marker  (sequencing not included) 250,00
2052 Cloning of a third DNA fragment into a vector with the same selection marker    (sequencing not included) 225,00
2060 Introduction of appropriate restriction sites on both ends      (sequencing not included) 300,00
           
  C. Cloning of amplified DNA fragments (from plasmids or genomic DNA) up to 5 kb    
2110 Cloning of a amplified DNA fragment into a vector of your choice.           (sequencing and primers not included) 350,00
2111 Cloning of a second amplified DNA fragment into the same vector      (sequencing and primers not included) 300,00
2112 Cloning of three amplified DNA fragments into the same vector      (sequencing and primers not included) 275,00
2120 or cloning of the same amplified DNA fragment into another vector      (sequencing and primers not included) 300,00
2121 or cloning of the same amplified DNA fragment into two other vectors      (sequencing and primers not included) 275,00
2130 Cloning of 1 nickase construct      (sequencing and primers included) 400,00
2131 Cloning of 2 nickase constructs into the same vector      (sequencing and primers included) 700,00
2132 Cloning of 3 nickase constructs into the same vector      (sequencing and primers included) 975,00
2140 Cloning of amplified DNA into a predigested vector      (sequencing not included) 300,00
2160 Cloning  of amplified DNA into a TA cloning vector     1 sample, sequencing of two clones included 250,00
2161 Cloning  of amplified DNA into a TA cloning vector     2-3 samples, sequencing of two clones included 200,00
2162 Cloning  of amplified DNA into a TA cloning vector     4-9 samples,sequencing of two clones included 175,00
2170 PCR-Cloning of DNA fragments larger 3.500 bp (1,00 € per 100 bp)      (sequencing and primers not included) 350,00
2180 Cloning  of amplified DNA into a pUC cloning vector      (sequencing not included) 300,00
           
  D. Site directed mutagenesis and DNA assembling, Gene Synthesis      
2200 Site directed mutagenesis from plasmids (1 point mutation)      (sequencing and primers not included) 250,00
2205 Site directed mutagenesis from plasmids (1 complex mutation)      (sequencing and primers not included) 300,00
2210 Site directed mutagenesis from plasmids ( a second clone)      (sequencing and primers not included) 200,00
2220 Site directed mutagenesis from plasmids (2 point mutations)      (sequencing and primers not included) 400,00
2230 Site directed mutagenesis from plasmids (3 point mutations)      (sequencing and primers not included) 500,00
2240 Site directed mutagenesis from plasmids (4 point mutations)      (sequencing and primers not included) 600,00
2300 Assembling of 2 DNA fragments and cloning into a vector of your choice    (sequencing not included) 450,00
2301 Assembling of 3 DNA fragments and cloning into a vector of your choice    (sequencing not included) 550,00
2302 Assembling of 4 DNA fragments and cloning into a vector of your choice    (sequencing not included) 650,00
2303 Assembling of 5 DNA fragments and cloning into a vector of your choice    (sequencing not included) 750,00
2310 Assembling of 2 DNA fragments    (sequencing and primers not included) 100,00
2400 Cloning of a amplified DNA fragment into a specific amplified vector    (sequencing and primers not included) 450,00
2500 Cloning of a DNA fragment into a vector with specific restriction enzymes     350,00
2900 Cloning of RVD into binary Talen construct     50,00
           
  E. Additional costs for cloning: If neccessary        
3090 Selection of bacteria with uncommon selection marker hygromycine   50,00
3090 Cloning into  E. coli strains other  than XL1       50,00
3105 Cloning of plasmids with missing sequence       50,00
3110 Cloning into low-copy plasmids additionally        50,00
3125 Cloning of DNA fragments larger 2.5 kb       35,00
3126 Cloning of DNA fragments larger 5 kb       70,00
3127 Cloning of DNA fragments larger 7.5 kb       105,00
3128 Cloning of DNA fragments larger 10 kb       140,00
3130 Blunt-end cloning one site        50,00
3145 Cloning into a single restriction site without blue-white selection.       50,00
3150 Cloning of a DNA fragment smaller 100 bp       25,00
3160 Cloning into vectors larger 10 kb       25,00
3170 In frame cloning       35,00
3180 Blunting of a restriction site       50,00
3190 Restriction of DNA fragments with uncommon or expensive enzymes like:
AgeI, AvrII, PciI, SphI, XmaI, ClaI- each site
    costs of the Enzymes  
3195 Isolation of a second clone       25,00
           
  Primer        
3210 Primer for DNA amplification and sequencing per base        0,50
3220 HPLC-purified primer per base (recomended for primers >30 bp)       1,00
3230 Phosphorylated primer per base       1,00
3240 Primer design       12,00
3241 Primer design and synthesis for sequencing       20,00
           
  F. Additional service        
  Cloning        
4100 Genesynthesis with primers per base and cloning into a basic pUC vector for genes smaller 625 bp 250,00
4110 Genesynthesis with primers per base and cloning into a basic pUC vector for genes larger 625 bp 0,35
4150 cDNA synthesis from mRNA       500,00
4160 5´Race     sequencing included 700,00
4170 3´Race     sequencing included 500,00
4210 Amplification and cloning of unknown genes with degenerated primers     on request
4310 Promotor isolation from cDNA-sequences -Isolation of genomic sequences with inverse PCR-     1 sample 1500,00
4311 Promotor isolation from cDNA-sequences -Isolation of genomic
sequences with inverse PCR-
    2 samples 1000,00
4410 Preparation of a cloning plan      each cloning step 50,00
4510 Documentation       10,00
4511 Certification       100,00
           
  Transformation/Amplification        
4600 Transformation of DNA into E. coli     1 sample 25,00
4601 Transformation of DNA into E. coli     2-9 samples 20,00
4602 Transformation of DNA into E. coli     >10 samples 15,00
4610 Streaking bacteria on a agar plate for antibiotic selection     1-10 samples 10,00
4611 Streaking bacteria on a agar plate for antibiotic selection     >10 samples 5,00
4630 Transformation of DNA into Agrobacterium and restriction digest     1 sample 100,00
4631 Transformation of DNA into Agrobacterium and restriction digest     2 samples 75,00
4710 Amplification of DNA with primers     1 sample 20,00
4711 Amplification of DNA with primers     >10 samples 10,00
4720 High quality amplification from genomic DNA     1 sample 100,00
           
  DNA/RNA isolation        
4800 Isolation of DNA/RNA from cells-Mini     1 sample 100,00
4801 Isolation of DNA/RNA from cells-Maxi     1 sample 200,00
4802 Isolation of DNA/RNA from cells-Mini     100 samples 20,00
4803 Pesteling of probes     100 samples 15,00
4804 RNA extraction     100 samples 10,00
4805 Probe preparation for extraction     100 samples 5,00
4820 Agarose gel purification     1 sample 50,00
4821 Agarose gel purification     >10 samples 20,00
4830 Spin column purification     1 sample 10,00
4840 Restriction digest     1 sample 5,00
4845 UV measurement at 260 nm and DNA adjustment     1 sample 10,00
4850 UV measurement at 260 nm      1 sample 5,00
4855 Adjustment of DNA to 10 ng/µl and filling in 5-10 x 1ml portions     1 sample 100,00
4860 Agarose gel analysis     1 sample 5,00
4900 E. coli Miniprep      1 sample 20,00
4901 E. coli Miniprep      2-10 samples 15,00
4902 E. coli Miniprep      >10 samples 10,00
4905 Colony PCR     >10 samples 5,00
4920 Isolation of Maxi-Prep DNA (up to 500 µg)     1 sample 95,00
4920.1 Isolation of Maxi-Prep DNA  and removal of genomic E. coli DNA with certification(100-200µg) 1 sample 390,00
4921 Isolation of Maxi-Prep DNA (up to 500 µg)     2-3 samples 85,00
4922 Isolation of Maxi-Prep DNA (up to 500 µg)     >3 samples 75,00
4930 Isolation of Mega-Prep DNA (up to 2.000 µg)     1 sample 240,00
4930.1 Isolation of Mega-Prep DNA  and removal of genomic E. coli DNA with certification(400-800µg) 1 sample 690,00
4931 Isolation of Mega-Prep DNA (up to 2.000 µg)     2 samples 180,00
4932 Isolation of Mega-Prep DNA (up to 2.000 µg)     20 samples 120,00
4940 Isolation of Giga-Prep DNA (up to 10.000 µg)     1 sample 700,00
4940.1 Isolation of Giga-Prep DNA  and removal of genomic E. coli DNA with certification(2-4mg) 1 sample 1250,00
4943 Isolation of Giga-Prep DNA (up to 10.000 µg) Endotoxin-free     1 sample 540,00
4950 Isolation of 60mg DNA      1 sample 3000,00
           
  G. Sequencing price per sample (average reading length: 5-600bp/99%)    
5105 Sequencing primer     1 primer 10,00
5106 Primer for Gene synthesis (30 bp)     1 primer 15,00
5110 Sequencing: normal     1-10 samples 10,00
5120 Sequencing: normal     11-100 samples 9,00
5130 Sequencing: normal     101-1000 samples 8,00
5410 Sequencing and comparison with a known sequence or storage of the sequence each sample 15,00
5415 Comparison with a known sequence      each sample 5,00
5421 Full documentation of the plasmid in gene bank format       50,00
5430 Import of sequences from NCBI       10,00
5510 DNA isolation, amplification and sequencing of genes from genomic DNA (16S-RNA, rubisco etc.) 200,00
           
  H. Supercompetent cells from your E. coli strain        
6110 Preparation of supercompetent cells from your E. coli strainin 2 ml vials (Transformation rate in 2ml vials: 1x109)     128 vials 320,00
6112 Preparation of supercompetent cells from your E. coli strain in 2 ml vials (Transformation rate in 2ml vials: 1x109)     64 vials 160,00
           
  Setup costs:        
  When amplification failed:       costs for primer
  When cloning failed:              Due to our experience 99% of our cloning experiments are succesful 50 % of cloning costs
           
  miscellaneous        
10001 Delivery on ice       20,00
10001 Delivery on dry ice       20,00
10002 Delivery with FedEx       50-100
10004 payment with cheque                 10,00
10005 payment with PayPal in Euro       5%
10006 PayPal payment in other currencies       10%
10006 payment with  wire transfer       free
10007 filling of clones for transportation     each sample 10

General Recommendations:

  • Prices are without 19% VAT
  • If you send us plasmid DNA for cloning, we prefer it purified by a midi/maxi prep in TE-buffer
  • We need  a minimum of 2µg vector DNA at a minimum concentration of 200ng/µl
  • Other plasmids should be already transformed in E. coli on a agar plate.
  • As a host strain we prefer DH5-alpha, XL1-blue or JM109
  • Generel cloning conditions will be valid for restriction enzymes with an overhang of
  • at least 3 bases, DNA fragments up to 2.000 bp and high-copy plasmids.
  • We don´t take any guaranty for genomic sequences
  • DNA data  should be readable by a computer