1. Sfil A and B restriction sites
Oriented cloning of genes from pUC based vectors into binaries can be done with one restriction enzyme. The unique 8 base Sfil restriction site at the border of the MCS makes it possible to switch easily between different vectors with different plant selection markers.
2. PUC-based expression cassettes
Cloning of the genes can be easily done in pUC-based vectors with different orientation of the MCS and blue-white selection.
3. Selection marker at LB
The selection marker is located at the left border of the binary.
4. Reduced interference of promoter-fusion expression with the 35S-enhancer
The 35S promoter, which drives the selection marker is located close to the left border. Promoter-fusions with detectable markers (GUS,GFP) cloned into the MCS at the right border will have reduced enhancement of expression from the 35S-enhancer compared to other vectors (Cambia).
5. Plant-Selection-Markers: Neomycin, Hygromycin, Phosphinotricin (bar)
Beside nptll (pLH9000+pLH9500) there are also binaries available with bar (pLH7000+pLH7500) and hpt (pLH6000) as a selection marker. So a second and third transformation of the transgenic plant is possible with a different selection marker.
The most exceptional feature of these vectors is the pVS1-ori which makes the binary more stable in Agrobacteria. This leds to a higher transformation rate compared to the pBin19 vector.
7. Bacterial selection marker: Streptomycin/Spectinomycin
For selection of plasmids in bacteria Streptomycin/Spectinomycin can be used.
The ColE1-ori allows elevated copy numbers in bacteria.
The construction of the LH vectors was published in German language only. If you have questions concerning these vectors feel free to send us a mail.