Short description of the pLH vectors
1. Sfil A and B restriction sites
Oriented cloning of genes from pUC based vectors into binaries can be done with one restriction enzyme. The unique 8 base Sfil restriction site at the border of the MCS makes it possible to switch easily between different vectors with different plant selection markers.
2. PUC-based expression cassettes
Description of the double promoter constructs for RNAi
Revolution in plant RNAi
inverted promoter instead of inverted DNA-fragments
Double stranded RNA is triggering a RNAi effect which can result in gene silencing in eucaryotic organisms. In these vectors the sense and antisense strand are produced constitutively, developmental specific or tissue specific by two inverted promoters. All double promoter constructs can be inserted into binary vectors with SfiI.
Vectors for inducible expression and inducible RNAi
Basic pUC vectors
Vectors with inverted double promoters for RNAi
puc vectors
At the moment we have three double promoter cassettes which can be cloned into all binaries by SfiI cloning.