DNA Cloning Service

1. Sfil A and B restriction sites

Oriented cloning of genes from pUC based vectors into binaries can be done with one restriction enzyme. The unique 8 base Sfil restriction site at the border of the MCS makes it possible to switch easily between different vectors with different plant selection markers.

2. PUC-based expression cassettes

DCS Binaries improved

http://dna-cloning.com/skripte/bin_imp/bin_imp.html

DCS Rnai vectors

http://dna-cloning.com/skripte/rnai/rnai.html

Description of the double promoter constructs for RNAi

Revolution in plant RNAi

inverted promoter instead of inverted DNA-fragments

Double stranded RNA is triggering a RNAi effect which can result in gene silencing in eucaryotic organisms. In these vectors the sense and antisense strand are produced constitutively, developmental specific or tissue specific by two inverted promoters. All double promoter constructs can be inserted into binary vectors with SfiI.

Vectors for inducible expression and inducible RNAi

Basic pUC vectors

Vectors with inverted double promoters for RNAi

puc vectors

At the moment we have three double promoter cassettes which can be cloned into all binaries by SfiI cloning.

Binaries with Sfil restriction sites

pUC based vectors with Sfil restriction sites

Short description of the pLH vectors