General

Nr. Euro
1000 Vectors from the website 200
1001 Special vectors from the website 300
1002 Vectors adapted to customers on request
1003 RNAi or recombinase vectors 250
1004 Agrobacterium strain GV3101-pMP90RK 200
1005 Cas9 vectors 400

Cloning from Plasmids (sequencing not included)

Nr. Euro
2010 Cloning of a DNA fragment into a vector of your choice. 250
2020 Cloning of a second DNA fragment into the same vector 200
2021 Cloning of a third DNA fragment into the same vector 175
2030 or cloning of the same DNA fragment into another vector 200
2040 or cloning of a DNA fragment into a predigested vector 200
2050 Cloning of a DNA fragment into a vector with the same selection marker 300
2051 Cloning of a second DNA fragment into a vector with the same selection marker 250
2052 Cloning of a third DNA fragment into a vector with the same selection marker 225
2060 Introduction of appropriate restriction sites on both ends 300

Cloning of amplified DNA fragments (from plasmids or genomic DNA up to 5 kb)

Nr. Euro
2110 Cloning of a amplified DNA fragment into a vector of your choice [1] 350
2111 Cloning of a second amplified DNA fragment into the same vecto. [1] 300
2112 Cloning of three amplified DNA fragments into the same vector [1] 275
2120 or cloning of the same amplified DNA fragment into another vector [1] 300
2121 or cloning of the same amplified DNA fragment into two other vectors [1] 275
2140 Cloning of amplified DNA into a predigested vector [2] 300
2160 Cloning of amplified DNA into a TA cloning vector (1 sample) 250
2161 Cloning of amplified DNA into a TA cloning vector (2-3 samples) 200
2162 Cloning of amplified DNA into a TA cloning vector (4-9 samples) 175
2170 PCR-Cloning of DNA fragments larger 3.500 bp (1,00per 100 bp) [1] 350
2180 Cloning of amplified DNA into a pUC cloning vector [2] 300

[1]: sequencing and primers not included
[2]: sequencing not included

Site directed mutagenesis, DNA assembling and Gene Synthesis

Nr. Euro
2200 Site directed mutagenesis from plasmids (1 point mutation) [1] 300
2205 Site directed mutagenesis from plasmids (1 complex mutation) [1] 350
2210 Site directed mutagenesis from plasmids ( a second clone) [1] 250
2220 Site directed mutagenesis from plasmids (2 point mutations) [1] 450
2230 Site directed mutagenesis from plasmids (3 point mutations) [1] 550
2240 Site directed mutagenesis from plasmids (4 point mutations) [1] 650
2300 Assembling of 2 DNA fragments and cloning into a vector of your choice [1] 450
2301 Assembling of 3 DNA fragments and cloning into a vector of your choice [1] 550
2302 Assembling of 4 DNA fragments and cloning into a vector of your choice [1] 650
2303 Assembling of 5 DNA fragments and cloning into a vector of your choice [1] 750
2310 Assembling of 2 DNA fragments [1] 100
2400 Cloning of a amplified DNA fragment into a specific amplified vector [1] 450
2500 Cloning of a DNA fragment into a vector with specific restriction enzymes 350
2600 Cloning of 1 target site into a Cas9 vector [2] 350
2601 Cloning of a second target site into a Cas9 vector [2] 300
2602 Cloning of a third target site into a Cas9 vector [2] 275
2610 Cloning of 1 double target site into a Cas9 vector [2] 400
2611 Cloning of 2 double target sites into a Cas9 vector [2] 350
2612 Cloning of 4 double target sites into a Cas9 vector [2] 325
2613 Cloning of 8 double target sites into a Cas9 vector [2] 300
2700 Cloning of 1 fourfold target sites into a Cas9 vector [2] 1,200
2800 Cloning of 1 sixfold target sites into a Cas9 vector [2] 2,000
2900 Cloning of RVD into binary Talen construct 50

[1]: sequencing and primers not included
[2]: sequencing and primers included

Optional cloning costs

Nr. Euro
3090 Selection of bacteria with uncommon selection marker (hygromycine) 50
3090 Cloning into E. coli strains other than XL1 50
3105 Cloning of plasmids with missing sequence 50
3110 Cloning into low-copy plasmids additionally 50
3125 Cloning of DNA fragments larger 2.5 kb 50
3126 Cloning of DNA fragments larger 5 kb 70
3127 Cloning of DNA fragments larger 7.5 kb 105
3128 Cloning of DNA fragments larger 10 kb 140
3130 Blunt-end cloning one site 50
3145 Cloning into a single restriction site without blue-white selection. 50
3150 Cloning of a DNA fragment smaller 100 bp 25
3160 Cloning into vectors larger 10 kb 25
3170 In frame cloning 35
3180 Blunting of a restriction site 50
3190 Restriction of DNA fragments with uncommon or expensive enzymes like: AgeI, AvrII, PciI, SphI, XmaI, ClaI - each site [1]
3195 Isolation of a second clone 25

[1]: costs of the Enzymes

Primer

Nr. Euro
3210 Primer for DNA amplification and sequencing per base 0.50
3220 HPLC-purified primer per base (recomended for primers >30 bp) 1
3230 Phosphorylated primer per base 1
3240 Primer design 12
3241 Primer design and synthesis for sequencing 20

Cloning

Nr. Euro
4100 Genesynthesis with primers per base and cloning into a basic pUC vector (for genes smaller 625 bp) 250
4110 Genesynthesis with primers per base and cloning into a basic pUC vector (for genes larger 625 bp) 0.35
4150 cDNA synthesis from mRNA 500
4160 5´Race [1] 700
4170 3´Race [1] 500
4210 Amplification and cloning of unknown genes with degenerated primers on request
4310 Promotor isolation from cDNA-sequences -Isolation of genomic sequences with inverse PCR- 1 sample 1,500
4311 Promotor isolation from cDNA-sequences -Isolation of genomic
sequences with inverse PCR-
2 samples 1,000
4410 Preparation of a cloning plan each cloning step 50
4510 Documentation 50
4511 Certification 100
4512 Preparation of electronic files due to changed sequences 100

[1]: sequencing included

Transformation and Amplification

Nr. Euro
4600 Transformation of DNA into E. coli 1 sample 25
4601 Transformation of DNA into E. coli 2-9 samples 20
4602 Transformation of DNA into E. coli >10 samples 15
4610 Streaking bacteria on a agar plate for antibiotic selection 1-10 samples 10
4611 Streaking bacteria on a agar plate for antibiotic selection >10 samples 5
4630 Transformation of DNA into Agrobacterium with sequencing of retransformed E.coli 1 sample 150
4631 Transformation of DNA into Agrobacterium with sequencing of retransformed E.coli 2 samples 100
4710 Amplification of DNA with primers 1 sample 20
4711 Amplification of DNA with primers >10 samples 10
4720 High quality amplification from genomic DNA 1 sample 100

DNA and RNA isolation

Nr. Euro
4800 Isolation of DNA/RNA from cells-Mini 1 sample 100
4801 Isolation of DNA/RNA from cells-Maxi 1 sample 200
4802 Isolation of DNA/RNA from cells-Mini 100 samples 20
4803 Pesteling of probes 100 samples 15
4804 RNA extraction 100 samples 10
4805 Probe preparation for extraction 100 samples 5
4820 Agarose gel purification 1 sample 50
4821 Agarose gel purification >10 samples 20
4830 Spin column purification 1 sample 10
4840 Restriction digest 1 sample 5
4845 UV measurement at 260 nm and DNA adjustment 1 sample 10
4850 UV measurement at 260 nm 1 sample 5
4855 Adjustment of DNA to 5 ng/µl 1 sample 100
4870 Dilution of DNA to 1x106 copys each sample 100
4880 Filling in tubes each sample 20
4890 Storage of the DNA each sample 100
4860 Agarose gel analysis 1 sample 10
4880 Removal of genomic E. coli DNA from Maxiprep 1 sample 200
4900 E. coli Miniprep 1 sample 20
4901 E. coli Miniprep 2-10 samples 15
4902 E. coli Miniprep >10 samples 10
4905 Colony PCR >10 samples 5
4920 Isolation of Maxi-Prep DNA (up to 500 µg) 1 sample 140
4921 Isolation of Maxi-Prep DNA (up to 500 µg) 2 sample 130
4922 Isolation of Maxi-Prep DNA (up to 500 µg) 3 sample 125
4923 Isolation of Maxi-Prep DNA (up to 500 µg) 4 sample 120
4924 Isolation of Maxi-Prep DNA (up to 500 µg) 6 sample 115
4925 Isolation of Maxi-Prep DNA (up to 500 µg) 8 sample 110
4930 Isolation of Mega-Prep DNA (up to 20 µg) 1 sample 295
4931 Isolation of Mega-Prep DNA (up to 20 µg) 2 samples 265
4940 Isolation of Giga-Prep DNA (up to 100 µg) 1 sample 1,000
4950 Isolation of 60mg DNA 1 sample 3,000

Sequencing price per sample (average reading length: 20-1000bp/99%)

Nr. Euro
5105 Sequencing primer 1 primer 10
5106 Primer for Gene synthesis (30 bp) 1 primer 15
5110 Sequencing: normal 1-10 samples 10
5120 Sequencing: normal 11-100 samples 9
5130 Sequencing: normal 101-1000 samples 8
5410 Sequencing and comparison with a known sequence or storage of the sequence each sample 15
5415 Comparison with a known sequence each sample 5
5421 Full documentation of the plasmid in gene bank format 50
5422 Sequencing of unknown DNA and documentation 1000 bp 100
5430 Import of sequences from NCBI 10
5510 DNA isolation, amplification and sequencing of genes from genomic DNA (16S-RNA, rubisco etc.) 200

Supercompetent cells from your E. coli Strain

Euro Euro
6110 Preparation of supercompetent cells from your E. coli strain in 2 ml vials (Transformation rate in 2ml vials: 1x109) 128 vials 384
6112 Preparation of supercompetent cells from your E. coli strain in 2 ml vials (Transformation rate in 2ml vials: 1x109) 64 vials 192

Plant transformation

Nr. Euro
7001 Transformation into Arabidopsis (T1 seeds tested) 750
7001 Transformation into Arabidopsis (T2 seeds) 1,250

Miscellaneous

Nr. Euro
10001 Delivery on ice 20
10001 Delivery on dry ice 20
10002 Delivery with FedEx 50-100
10003 Delivery with Expeditors 150
10004 payment with cheque 10
10005 payment with PayPal in Euro 5%
10006 PayPal payment in other currencies 10%
10006 payment with wire transfer free
10007 filling of clones for transportation each sample 10