LOCUS B401p9oN-LH 9317 bp DNA circular SYN 22-JAN-2016 DEFINITION Ligation of npt-xho-mlu* into 715p7N-TE9* ACCESSION B401p9oN-LH KEYWORDS . SOURCE Unknown. ORGANISM Unknown Unclassified. REFERENCE 1 (bases 1 to 9317) AUTHORS Self JOURNAL Unpublished. COMMENT SECID/File created by Clone Manager, Scientific & Educational Software COMMENT SECNOTES|Vector molecule: 715p7N-TE9* Fragment ends: MluI and XhoI Fragment size: 8306 Insert molecule: npt-xho-mlu* Fragment ends: XhoI and MluI Fragment size: 1011 Primer for gene synthesis nptXho CCCGCctcgagTCCTCCAtaactATGGGAGCATGGATTGA nptMlu cccgACGCGTtcaGAAGAACTCGTCAAGC Sequencing primers RBCS-TR2 CACAGTAAATTACAAGCACAAC LHX1 CTTTATTTCGACGTGTCTAC FEATURES Location/Qualifiers misc_feature 1..24 /gene="BL" /product="left border" /SECDrawAs="Region" /SECStyleId=1 misc_feature 309..606 /gene="Pnos" /product="Promotor" /SECDrawAs="Region" /SECStyleId=1 CDS 625..923 /gene="nptIIo" /product="NptII optimiert" /SECDrawAs="Gene" /SECStyleId=1 misc_signal 920..923 /label=splice /SECDrawAs="Label" CDS 924..1110 /gene="int" /SECDrawAs="Gene" /SECStyleId=1 misc_signal 1109..1112 /label=splice /SECDrawAs="Label" CDS 1111..1614 /gene="nptIIo" /product="NptII optimiert" /SECDrawAs="Gene" /SECStyleId=1 misc_feature 1628..1918 /gene="TE9" /product="Terminator Rbcs-E9" /SECDrawAs="Region" /SECStyleId=1 misc_feature 2094..2117 /gene="BR" /product="right border" /SECDrawAs="Region" /SECStyleId=1 misc_feature 2652..3349 /gene="ColE1" /product="ColE1 ORI" /SECDrawAs="Region" /SECStyleId=1 misc_feature 3898..7603 /gene="pVS1 ORI" /product="pVS1 ORI" /SECDrawAs="Region" /SECStyleId=1 CDS 7787..8797 /gene="Sm/Sp" /product="Strep/Spec resistence" /SECDrawAs="Gene" /SECStyleId=1 ORIGIN 1 ggcaggatat attcaattgt aaatggcttc atgtccggga aatctacatg gatcagcaat 61 gagtatgatg gtcaatatgg agaaaaagaa agagtaatta ccaatttttt ttcaattcaa 121 aaatgtagat gtccgcagcg ttattataaa atgaaagtac attttgataa aacgacaaat 181 tacgatccgt cgtatttata ggcgaaagca ataaacaaat tattctaatt cggaaatctt 241 tatttcgacg tgtctacatt cacgtccaaa tgggggctta gatgagaaac ttcacgatcg 301 gctctagatc atgagcggag aattaaggga gtcacgttat gacccccgcc gatgacgcgg 361 gacaagccgt tttacgtttg gaactgacag aaccgcaacg ttgaaggagc cactcagccg 421 cgggtttctg gagtttaatg agctaagcac atacgtcaga aaccattatt gcgcgttcaa 481 aagtcgccta aggtcactat cagctagcaa atatttcttg tcaaaaatgc tccactgacg 541 ttccataaat tcccctcggt atccaattag agtctcatat tcactctcaa tccaaataat 601 ctgcacctcg agtcctccat aactatggga gcatggattg agcaggatgg cctccatgct 661 ggtagccccg ctgcttgggt ggagcgcctc tttggctacg actgggccca acagaccatt 721 gggtgcagtg atgccgctgt cttccgtttg agcgcccagg gcaggccagt tctcttcgtg 781 aagactgacc tctccggtgc cctcaacgag ttgcaggacg aagctgcccg cctctcatgg 841 cttgccacca ccggagtccc ttgcgctgcc gtgttggacg ttgtcacaga ggccggaaga 901 gactggctgc tcttgggcga ggtaagtttc tgcttctacc tttgatatat atataataat 961 tatcattaat tagtagtaat ataatatttc aaatattttt ttcaaaataa aagaatgtag 1021 tatatagcaa ttgcttttct gtagtttata agtgtgtata ttttaattta taacttttct 1081 aatatatgac caaaatttgt tgatgtgcag gtgccaggcc aagacctcct gagctcccac 1141 ctggccccag ccgagaaggt ctccatcatg gcagacgcca tgcgtcgcct ccacaccctg 1201 gacccagcca cctgcccctt cgaccaccag gccaagcaca ggatcgaacg tgcacgcact 1261 aggatggagg ccggccttgt ggaccaggat gaccttgacg aggagcacca gggccttgct 1321 cccgccgagc tgttcgcccg cctcaaggct agaatgcctg acggagagga cttggtggtc 1381 acccacgggg atgcctgcct ccccaacatc atggttgaga acggacgctt ctctggcttc 1441 atcgactgcg gtcgtctggg cgtggccgac cgctaccaag acatcgccct ggccaccagg 1501 gacatcgctg aggaacttgg aggggagtgg gccgatcgtt tcttggtcct ctacggcatt 1561 gctgcccccg actcccagag gatcgccttc taccgcctgc ttgacgagtt cttctgaacg 1621 cgttcgagta ttatggcatt gggaaaactg tttttcttgt accatttgtt gtgcttgtaa 1681 tttactgtgt tttttattcg gttttcgcta tcgaactgtg aaatggaaat ggatggagaa 1741 gagttaatga atgatatggt ccttttgttc attctcaaat taatattatt tgttttttct 1801 cttatttgtt gtgtgttgaa tttgaaatta taagagatat gcaaacattt tgttttgagt 1861 aaaaatgtgt caaatcgtgg cctctaatga ccgaagttaa tatgaggagt aaaacactga 1921 agcctgcagg catgcaagct gatccactag aggccatggc ggccgcacta gtggatcccc 1981 cgggatgcat gaattcaagc ttatcgatgt cgacaggcct taagggccag atcttgggcc 2041 cggtacccga tcagattgtc gtttcccgcc ttcggtttaa actatcagtg tttgacagga 2101 tatattggcg ggtaaaccta agagaaaaga gcgtttatta gaataatcgg atatttaaaa 2161 gggcgtgaaa aggtttatcc gttcgtccat ttgtatgtgc atgccaacca cagggttccc 2221 ctcgggagtg cttggcattc cgtgcgataa tgacttctgt tcaaccaccc aaacgtcgga 2281 aagcctgacg acggagcagc attccaaaaa gatcccttgg ctcgtctggg tcggctagaa 2341 ggtcgagtgg gctgctgtgg cttgatccct caacgcggtc gcggacgtag cgcagcgccg 2401 aaaaatcctc gatcgcaaat ccgacgctgt cgaaaagcgt gatctgcttg tcgctctttc 2461 ggccgacgtc ctggccagtc atcacgcgcc aaagttccgt cacaggatga tctggcgcga 2521 gttgctggat ctcgccttca atccgggtct gtggcgggaa ctccacgaaa atatccgaac 2581 gcagcaagat atcgcggtgc atctcggtct tgcctgggca gtcgccgccg acgccgttga 2641 tgtggacgcc gaaaaggatc taggtgaaga tcctttttga taatctcatg accaaaatcc 2701 cttaacgtga gttttcgttc cactgagcgt cagaccccgt agaaaagatc aaaggatctt 2761 cttgagatcc tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac 2821 cagcggtggt ttgtttgccg gatcaagagc taccaactct ttttccgaag gtaactggct 2881 tcagcagagc gcagatacca aatactgttc ttctagtgta gccgtagtta ggccaccact 2941 tcaagaactc tgtagcaccg cctacatacc tcgctctgct aatcctgtta ccagtggctg 3001 ctgccagtgg cgataagtcg tgtcttaccg ggttggactc aagacgatag ttaccggata 3061 aggcgcagcg gtcgggctga acggggggtt cgtgcacaca gcccagcttg gagcgaacga 3121 cctacaccga actgagatac ctacagcgtg agctatgaga aagcgccacg cttcccgaag 3181 ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg 3241 agcttccagg gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc cacctctgac 3301 ttgagcgtcg atttttgtga tgctcgtcag gggggcggag cctatggaaa aacgccagca 3361 acgcggcctt tttacggttc ctggcctttt gctggccttt tgctcacatg ttctttcctg 3421 cgttatcccc tgattctgtg gataaccgat taccgccttt gagtgagctg ataccgctcg 3481 ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaag agcgcctgat 3541 gcggtatttt ctccttacgc atctgtgcgg tatttcacac cgcatatggt gcactctcag 3601 tacaatctgc tctgatgccg catagttaag ccagtataca ctccgctatc gctacgtgac 3661 tgggtcatgg ctgcgccccg acacccgcca acacccgctg acgcgccctg acgggcttgt 3721 ctgctcccgg catccgctta cagacaagct gtgaccgtct ccgggagctg catgtgtcag 3781 aggttttcac cgtcatcacc gaaacgcgcg aggcaggggt acgtcgaggt cgatccaacc 3841 cctccgctgc tatagtgcag tcggcttctg acgttcagtg cagccgtctt ctgaaaacga 3901 catgtcgcac aagtcctaag ttacgcgaca ggctgccgcc ctgccctttt cctggcgttt 3961 tcttgtcgcg tgttttagtc gcataaagta gaatacttgc gactagaacc ggagacatta 4021 cgccatgaac aagagcgccg ccgctggcct gctgggctat gcccgcgtca gcaccgacga 4081 ccaggacttg accaaccaac gggccgaact gcacgcggcc ggctgcacca agctgttttc 4141 cgagaagatc accggcacca ggcgcgaccg cccggagctg gccaggatgc ttgaccacct 4201 acgccctggc gacgttgtga cagtgaccag gctagaccgc ctggcccgca gcacccgcga 4261 cctactggac attgccgagc gcatccagga ggccggcgcg ggcctgcgta gcctggcaga 4321 gccgtgggcc gacaccacca cgccggccgg ccgcatggtg ttgaccgtgt tcgccggcat 4381 tgccgagttc gagcgttccc taatcatcga ccgcacccgg agcgggcgcg aggccgccaa 4441 ggcgcgaggc gtgaagtttg gcccccgccc taccctcacc ccggcacaga tcgcgcacgc 4501 ccgcgagctg atcgaccagg aaggccgcac cgtgaaagag gcggctgcac tgcttggcgt 4561 gcatcgctcg accctgtacc gcgcacttga gcgcagcgag gaagtgacgc ccaccgaggc 4621 caggcggcgc ggtgccttcc gtgaggacgc attgaccgag gccgacgccc tggcggccgc 4681 cgagaatgaa cgccaagagg aacaagcatg aaaccgcacc aggacggcca ggacgaaccg 4741 tttttcatta ccgaagagat cgaggcggag atgatcgcgg ccgggtacgt gttcgagccg 4801 cccgcgcacg tctcaaccgt gcggctgcat gaaatcctgg ccggtttgtc tgatgccaag 4861 ctcgcggcct ggccggcgag cttggccgct gaagaaaccg agcgccgccg tctaaaaagg 4921 tgatgtgtat ttgagtaaaa cagcttgcgt catgcggtcg ctgcgtatat gatgcgatga 4981 gtaaataaac aaatacgcaa ggggaacgca tgaaggttat cgctgtactt aaccagaaag 5041 gcgggtcagg caagacgacc atcgcaaccc atctagcccg cgccctgcaa ctcgccgggg 5101 ccgatgttct gttagtcgat tccgatcccc agggcagtgc ccgcgattgg gcggccgtgc 5161 gggaagatca accgctaacc gttgtcggca tcgaccgccc gacgattgac cgcgacgtga 5221 aggccatcgg ccggcgcgac ttcgtagtga tcgacggagc gccccaggcg gcggacttgg 5281 ctgtgtccgc gatcaaggca gccgacttcg tgctgattcc ggtgcagcca agcccttacg 5341 acatatgggc caccgccgac ctggtggagc tggttaagca gcgcattgag gtcacggatg 5401 gaaggctaca agcggccttt gtcgtgtcgc gggcgatcaa aggcacgcgc atcggcggtg 5461 aggttgccga ggcgctggcc gggtacgagc tgcccattct tgagtcccgt atcacgcagc 5521 gcgtgagcta cccaggcact gccgccgccg gcacaaccgt tcttgaatca gaacccgagg 5581 gcgacgctgc ccgcgaggtc caggcgctgg ccgctgaaat taaatcaaaa ctcatttgag 5641 ttaatgaggt aaagagaaaa tgagcaaaag cacaaacacg ctaagtgccg gccgtccgag 5701 cgcacgcagc agcaaggctg caacgttggc cagcctggca gacacgccag ccatgaagcg 5761 ggtcaacttt cagttgccgg cggaggatca caccaagctg aagatgtacg cggtacgcca 5821 aggcaagacc attaccgagc tgctatctga atacatcgcg cagctaccag agtaaatgag 5881 caaatgaata aatgagtaga tgaattttag cggctaaagg aggcggcatg gaaaatcaag 5941 aacaaccagg caccgacgcc gtggaatgcc ccatgtgtgg aggaacgggc ggttggccag 6001 gcgtaagcgg ctgggttgtc tgccggccct gcaatggcac tggaaccccc aagcccgagg 6061 aatcggcgtg agcggtcgca aaccatccgg cccggtacaa atcggcgcgg cgctgggtga 6121 tgacctggtg gagaagttga aggcggcgca ggccgcccag cggcaacgca tcgaggcaga 6181 agcacgcccc ggtgaatcgt ggcaagcggc cgctgatcga atccgcaaag aatcccggca 6241 accgccggca gccggtgcgc cgtcgattag gaagccgccc aagggcgacg agcaaccaga 6301 ttttttcgtt ccgatgctct atgacgtggg cacccgcgat agtcgcagca tcatggacgt 6361 ggccgttttc cgtctgtcga agcgtgaccg acgagctggc gaggtgatcc gctacgagct 6421 tccagacggg cacgtagagg tttccgcagg gccggccggc atggcgagtg tgtgggatta 6481 cgacctggta ctgatggcgg tttcccatct aaccgaatcc atgaaccgat accgggaagg 6541 gaagggagac aagcccggcc gcgtgttccg tccacacgtt gcggacgtac tcaagttctg 6601 ccggcgagcc gatggcggaa agcagaaaga cgacctggta gaaacctgca ttcggttaaa 6661 caccacgcac gttgccatgc agcgtacgaa gaaggccaag aacggccgcc tggtgacggt 6721 atccgagggt gaagccttga ttagccgcta caagatcgta aagagcgaaa ccgggcggcc 6781 ggagtacatc gagatcgagc tagctgattg gatgtaccgc gagatcacag aaggcaagaa 6841 cccggacgtg ctgacggttc accccgatta ctttttgatc gatcccggca tcggccgttt 6901 tctctaccgc ctggcacgcc gcgccgcagg caaggcagaa gccagatggt tgttcaagac 6961 gatctacgaa cgcagtggca gcgccggaga gttcaagaag ttctgtttca ccgtgcgcaa 7021 gctgatcggg tcaaatgacc tgccggagta cgatttgaag gaggaggcgg ggcaggctgg 7081 cccgatccta gtcatgcgct accgcaacct gatcgagggc gaagcatccg ccggttccta 7141 atgtacggag cagatgctag ggcaaattgc cctagcaggg gaaaaaggtc gaaaaggtct 7201 ctttcctgtg gatagcacgt acattgggaa cccaaagccg tacattggga accggaaccc 7261 gtacattggg aacccaaagc cgtacattgg gaaccggtca cacatgtaag tgactgatat 7321 aaaagagaaa aaaggcgatt tttccgccta aaactcttta aaacttatta aaactcttaa 7381 aacccgcctg gcctgtgcat aactgtctgg ccagcgcaca gccgaagagc tgcaaaaagc 7441 gcctaccctt cggtcgctgc gctccctacg ccccgccgct tcgcgtcggc ctatcgcggc 7501 cgctggccgc tcaaaaatgg ctggcctacg gccaggcaat ctaccagggc gcggacaagc 7561 cgcgccgtcg ccactcgacc gccggcgccc acatcaaggc accggtgggt atgcctgacg 7621 atgcgtggag accgaaacct tgcgctcgtt cgccagccag gacagaaatg cctcgacttc 7681 gctgctgccc aaggttgccg ggtgacgcac accgtggaaa cggatgaagg cacgaaccca 7741 gtggacataa gcctgttcgg ttcgtaagct gtaatgcaag tagcgtatgc gctcacgcaa 7801 ctggtccaga accttgaccg aacgcagcgg tggtaacggc gcagtggcgg ttttcatggc 7861 ttgttatgac tgtttttttg gggtacagtc tatgcctcgg gcatccaagc agcaagcgcg 7921 ttacgccgtg ggtcgatgtt tgatgttatg gagcagcaac gatgttacgc agcagggcag 7981 tcgccctaaa acaaagttaa acatcatgag ggaagcggtg atcgccgaag tatcgactca 8041 actatcagag gtagttggcg tcatcgagcg ccatctcgaa ccgacgttgc tggccgtaca 8101 tttgtacggc tccgcagtgg atggcggcct gaagccacac agtgatattg atttgctggt 8161 tacggtgacc gtaaggcttg atgaaacaac gcggcgagct ttgatcaacg accttttgga 8221 aacttcggct tcccctggag agagcgagat tctccgcgct gtagaagtca ccattgttgt 8281 gcacgacgac atcattccgt ggcgttatcc agctaagcgc gaactgcaat ttggagaatg 8341 gcagcgcaat gacattcttg caggtatctt cgagccagcc acgatcgaca ttgatctggc 8401 tatcttgctg acaaaagcaa gagaacatag cgttgccttg gtaggtccag cggcggagga 8461 actctttgat ccggttcctg aacaggatct atttgaggcg ctaaatgaaa ccttaacgct 8521 atggaactcg ccgcccgact gggctggcga tgagcgaaat gtagtgctta cgttgtcccg 8581 catttggtac agcgcagtaa ccggcaaaat cgcgccgaag gatgtcgctg ccgactgggc 8641 aatggagcgc ctgccggccc agtatcagcc cgtcatactt gaagctagac aggcttatct 8701 tggacaagaa gaagatcgct tggcctcgcg cgcagatcag ttggaagaat ttgtccacta 8761 cgtgaaaggc gagatcacca aggtagtcgg caaataatgt ctaacaattc gttcaagccg 8821 acgccgcttc gcggcgcggc ttaactcaag cgttagatgc actaagcaca taattgctca 8881 cagccaaact atcaggtcaa gtctgctttt attattttta agcgtgcata ataagcccta 8941 cacaaattgg gagatatatc atgaaaggct ggctttttct tgttatcgca atagttggcg 9001 aagtaatcgc aacatagctt gcttggtcgt tccgcgtgaa cgtcggctcg attgtacctg 9061 cgttcaaata ctttgcgatc gtgttgcgcg cctgcccggt gcgtcggctg atctcacgga 9121 tcgactgctt ctctcgcaac gccatccgac ggatgatgtt taaaagtccc atgtggatca 9181 ctccgttgcc ccgtcgctca ccgtgttggg gggaaggtgc acatggctca gttctcaatg 9241 gaaattatct gcctaaccgg ctcagttctg cgtagaaacc aacatgcaag ctccaccggg 9301 tgcaaagcgg cagcggc //