LOCUS       B403p9oN-ocs-LH         9517 bp    DNA     circular SYN 22-JAN-2016
DEFINITION  Ligation of pOCS-BM* into B401p9oN-LH*
ACCESSION   B403p9oN-ocs-LH
KEYWORDS    .
SOURCE      Unknown.
  ORGANISM  Unknown
            Unclassified.
REFERENCE   1  (bases 1 to 9517)
  AUTHORS   Self
  JOURNAL   Unpublished.
COMMENT     SECID/File created by Clone Manager, Scientific & Educational Software
COMMENT     SECNOTES|Vector molecule:  B401p9oN-LH*
               Fragment ends: SfiI
               Fragment size: 9253
            Insert molecule:  pOCS-BM*
               Fragment ends: SfiI
               Fragment size: 264
            Sequencing primers	
            SBx	GGTCCTTTTGTTCATTCTCAA
FEATURES             Location/Qualifiers
     misc_feature    1..24
                     /gene="BL"
                     /product="left border"
                     /SECDrawAs="Region"
                     /SECStyleId=1
     misc_feature    309..606
                     /gene="Pnos"
                     /product="Promotor"
                     /SECDrawAs="Region"
                     /SECStyleId=1
     CDS             625..923
                     /gene="nptIIo"
                     /product="NptII optimiert"
                     /SECDrawAs="Gene"
                     /SECStyleId=1
     misc_signal     920..923
                     /label=splice
                     /SECDrawAs="Label"
     CDS             924..1110
                     /gene="int"
                     /SECDrawAs="Gene"
                     /SECStyleId=1
     misc_signal     1109..1112
                     /label=splice
                     /SECDrawAs="Label"
     CDS             1111..1614
                     /gene="nptIIo"
                     /product="NptII optimiert"
                     /SECDrawAs="Gene"
                     /SECStyleId=1
     misc_feature    1628..1918
                     /gene="TE9"
                     /product="Terminator Rbcs-E9"
                     /SECDrawAs="Region"
                     /SECStyleId=1
     misc_feature    2014..2209
                     /gene="Tocs"
                     /product="Terminator"
                     /SECDrawAs="Region"
                     /SECStyleId=1
                     /SECName="Tocs"
                     /SECDescr="Terminator"
     misc_feature    2294..2317
                     /gene="BR"
                     /product="right border"
                     /SECDrawAs="Region"
                     /SECStyleId=1
     misc_feature    2852..3549
                     /gene="ColE1"
                     /product="ColE1 ORI"
                     /SECDrawAs="Region"
                     /SECStyleId=1
     misc_feature    4098..7803
                     /gene="pVS1 ORI"
                     /product="pVS1 ORI"
                     /SECDrawAs="Region"
                     /SECStyleId=1
     CDS             7987..8997
                     /gene="Sm/Sp"
                     /product="Strep/Spec resistence"
                     /SECDrawAs="Gene"
                     /SECStyleId=1
ORIGIN      
        1 ggcaggatat attcaattgt aaatggcttc atgtccggga aatctacatg gatcagcaat
       61 gagtatgatg gtcaatatgg agaaaaagaa agagtaatta ccaatttttt ttcaattcaa
      121 aaatgtagat gtccgcagcg ttattataaa atgaaagtac attttgataa aacgacaaat
      181 tacgatccgt cgtatttata ggcgaaagca ataaacaaat tattctaatt cggaaatctt
      241 tatttcgacg tgtctacatt cacgtccaaa tgggggctta gatgagaaac ttcacgatcg
      301 gctctagatc atgagcggag aattaaggga gtcacgttat gacccccgcc gatgacgcgg
      361 gacaagccgt tttacgtttg gaactgacag aaccgcaacg ttgaaggagc cactcagccg
      421 cgggtttctg gagtttaatg agctaagcac atacgtcaga aaccattatt gcgcgttcaa
      481 aagtcgccta aggtcactat cagctagcaa atatttcttg tcaaaaatgc tccactgacg
      541 ttccataaat tcccctcggt atccaattag agtctcatat tcactctcaa tccaaataat
      601 ctgcacctcg agtcctccat aactatggga gcatggattg agcaggatgg cctccatgct
      661 ggtagccccg ctgcttgggt ggagcgcctc tttggctacg actgggccca acagaccatt
      721 gggtgcagtg atgccgctgt cttccgtttg agcgcccagg gcaggccagt tctcttcgtg
      781 aagactgacc tctccggtgc cctcaacgag ttgcaggacg aagctgcccg cctctcatgg
      841 cttgccacca ccggagtccc ttgcgctgcc gtgttggacg ttgtcacaga ggccggaaga
      901 gactggctgc tcttgggcga ggtaagtttc tgcttctacc tttgatatat atataataat
      961 tatcattaat tagtagtaat ataatatttc aaatattttt ttcaaaataa aagaatgtag
     1021 tatatagcaa ttgcttttct gtagtttata agtgtgtata ttttaattta taacttttct
     1081 aatatatgac caaaatttgt tgatgtgcag gtgccaggcc aagacctcct gagctcccac
     1141 ctggccccag ccgagaaggt ctccatcatg gcagacgcca tgcgtcgcct ccacaccctg
     1201 gacccagcca cctgcccctt cgaccaccag gccaagcaca ggatcgaacg tgcacgcact
     1261 aggatggagg ccggccttgt ggaccaggat gaccttgacg aggagcacca gggccttgct
     1321 cccgccgagc tgttcgcccg cctcaaggct agaatgcctg acggagagga cttggtggtc
     1381 acccacgggg atgcctgcct ccccaacatc atggttgaga acggacgctt ctctggcttc
     1441 atcgactgcg gtcgtctggg cgtggccgac cgctaccaag acatcgccct ggccaccagg
     1501 gacatcgctg aggaacttgg aggggagtgg gccgatcgtt tcttggtcct ctacggcatt
     1561 gctgcccccg actcccagag gatcgccttc taccgcctgc ttgacgagtt cttctgaacg
     1621 cgttcgagta ttatggcatt gggaaaactg tttttcttgt accatttgtt gtgcttgtaa
     1681 tttactgtgt tttttattcg gttttcgcta tcgaactgtg aaatggaaat ggatggagaa
     1741 gagttaatga atgatatggt ccttttgttc attctcaaat taatattatt tgttttttct
     1801 cttatttgtt gtgtgttgaa tttgaaatta taagagatat gcaaacattt tgttttgagt
     1861 aaaaatgtgt caaatcgtgg cctctaatga ccgaagttaa tatgaggagt aaaacactga
     1921 agcctgcagg catgcaagct gatccactag aggccatggc ggccgcacta gtggatcccc
     1981 cgggctgcag gaattcaagc ttacgcgtgt cgaccctgct ttaatgagat atgcgagacg
     2041 cctatgatcg catgatattt gctttcaatt ctgttgtgca cgttgtaaaa aacctgagca
     2101 tgtgtagctc agatccttac cgccggtttc ggttcattct aatgaatata tcacccgtta
     2161 ctatcgtatt tttatgaata atattctccg ttcaatttac tgattgtcct cgacaggcct
     2221 taagggccag atcttgggcc cggtacccga tcagattgtc gtttcccgcc ttcggtttaa
     2281 actatcagtg tttgacagga tatattggcg ggtaaaccta agagaaaaga gcgtttatta
     2341 gaataatcgg atatttaaaa gggcgtgaaa aggtttatcc gttcgtccat ttgtatgtgc
     2401 atgccaacca cagggttccc ctcgggagtg cttggcattc cgtgcgataa tgacttctgt
     2461 tcaaccaccc aaacgtcgga aagcctgacg acggagcagc attccaaaaa gatcccttgg
     2521 ctcgtctggg tcggctagaa ggtcgagtgg gctgctgtgg cttgatccct caacgcggtc
     2581 gcggacgtag cgcagcgccg aaaaatcctc gatcgcaaat ccgacgctgt cgaaaagcgt
     2641 gatctgcttg tcgctctttc ggccgacgtc ctggccagtc atcacgcgcc aaagttccgt
     2701 cacaggatga tctggcgcga gttgctggat ctcgccttca atccgggtct gtggcgggaa
     2761 ctccacgaaa atatccgaac gcagcaagat atcgcggtgc atctcggtct tgcctgggca
     2821 gtcgccgccg acgccgttga tgtggacgcc gaaaaggatc taggtgaaga tcctttttga
     2881 taatctcatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt cagaccccgt
     2941 agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct gctgcttgca
     3001 aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc taccaactct
     3061 ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgttc ttctagtgta
     3121 gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc tcgctctgct
     3181 aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg ggttggactc
     3241 aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt cgtgcacaca
     3301 gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg agctatgaga
     3361 aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg
     3421 aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt atagtcctgt
     3481 cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag gggggcggag
     3541 cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt gctggccttt
     3601 tgctcacatg ttctttcctg cgttatcccc tgattctgtg gataaccgat taccgccttt
     3661 gagtgagctg ataccgctcg ccgcagccga acgaccgagc gcagcgagtc agtgagcgag
     3721 gaagcggaag agcgcctgat gcggtatttt ctccttacgc atctgtgcgg tatttcacac
     3781 cgcatatggt gcactctcag tacaatctgc tctgatgccg catagttaag ccagtataca
     3841 ctccgctatc gctacgtgac tgggtcatgg ctgcgccccg acacccgcca acacccgctg
     3901 acgcgccctg acgggcttgt ctgctcccgg catccgctta cagacaagct gtgaccgtct
     3961 ccgggagctg catgtgtcag aggttttcac cgtcatcacc gaaacgcgcg aggcaggggt
     4021 acgtcgaggt cgatccaacc cctccgctgc tatagtgcag tcggcttctg acgttcagtg
     4081 cagccgtctt ctgaaaacga catgtcgcac aagtcctaag ttacgcgaca ggctgccgcc
     4141 ctgccctttt cctggcgttt tcttgtcgcg tgttttagtc gcataaagta gaatacttgc
     4201 gactagaacc ggagacatta cgccatgaac aagagcgccg ccgctggcct gctgggctat
     4261 gcccgcgtca gcaccgacga ccaggacttg accaaccaac gggccgaact gcacgcggcc
     4321 ggctgcacca agctgttttc cgagaagatc accggcacca ggcgcgaccg cccggagctg
     4381 gccaggatgc ttgaccacct acgccctggc gacgttgtga cagtgaccag gctagaccgc
     4441 ctggcccgca gcacccgcga cctactggac attgccgagc gcatccagga ggccggcgcg
     4501 ggcctgcgta gcctggcaga gccgtgggcc gacaccacca cgccggccgg ccgcatggtg
     4561 ttgaccgtgt tcgccggcat tgccgagttc gagcgttccc taatcatcga ccgcacccgg
     4621 agcgggcgcg aggccgccaa ggcgcgaggc gtgaagtttg gcccccgccc taccctcacc
     4681 ccggcacaga tcgcgcacgc ccgcgagctg atcgaccagg aaggccgcac cgtgaaagag
     4741 gcggctgcac tgcttggcgt gcatcgctcg accctgtacc gcgcacttga gcgcagcgag
     4801 gaagtgacgc ccaccgaggc caggcggcgc ggtgccttcc gtgaggacgc attgaccgag
     4861 gccgacgccc tggcggccgc cgagaatgaa cgccaagagg aacaagcatg aaaccgcacc
     4921 aggacggcca ggacgaaccg tttttcatta ccgaagagat cgaggcggag atgatcgcgg
     4981 ccgggtacgt gttcgagccg cccgcgcacg tctcaaccgt gcggctgcat gaaatcctgg
     5041 ccggtttgtc tgatgccaag ctcgcggcct ggccggcgag cttggccgct gaagaaaccg
     5101 agcgccgccg tctaaaaagg tgatgtgtat ttgagtaaaa cagcttgcgt catgcggtcg
     5161 ctgcgtatat gatgcgatga gtaaataaac aaatacgcaa ggggaacgca tgaaggttat
     5221 cgctgtactt aaccagaaag gcgggtcagg caagacgacc atcgcaaccc atctagcccg
     5281 cgccctgcaa ctcgccgggg ccgatgttct gttagtcgat tccgatcccc agggcagtgc
     5341 ccgcgattgg gcggccgtgc gggaagatca accgctaacc gttgtcggca tcgaccgccc
     5401 gacgattgac cgcgacgtga aggccatcgg ccggcgcgac ttcgtagtga tcgacggagc
     5461 gccccaggcg gcggacttgg ctgtgtccgc gatcaaggca gccgacttcg tgctgattcc
     5521 ggtgcagcca agcccttacg acatatgggc caccgccgac ctggtggagc tggttaagca
     5581 gcgcattgag gtcacggatg gaaggctaca agcggccttt gtcgtgtcgc gggcgatcaa
     5641 aggcacgcgc atcggcggtg aggttgccga ggcgctggcc gggtacgagc tgcccattct
     5701 tgagtcccgt atcacgcagc gcgtgagcta cccaggcact gccgccgccg gcacaaccgt
     5761 tcttgaatca gaacccgagg gcgacgctgc ccgcgaggtc caggcgctgg ccgctgaaat
     5821 taaatcaaaa ctcatttgag ttaatgaggt aaagagaaaa tgagcaaaag cacaaacacg
     5881 ctaagtgccg gccgtccgag cgcacgcagc agcaaggctg caacgttggc cagcctggca
     5941 gacacgccag ccatgaagcg ggtcaacttt cagttgccgg cggaggatca caccaagctg
     6001 aagatgtacg cggtacgcca aggcaagacc attaccgagc tgctatctga atacatcgcg
     6061 cagctaccag agtaaatgag caaatgaata aatgagtaga tgaattttag cggctaaagg
     6121 aggcggcatg gaaaatcaag aacaaccagg caccgacgcc gtggaatgcc ccatgtgtgg
     6181 aggaacgggc ggttggccag gcgtaagcgg ctgggttgtc tgccggccct gcaatggcac
     6241 tggaaccccc aagcccgagg aatcggcgtg agcggtcgca aaccatccgg cccggtacaa
     6301 atcggcgcgg cgctgggtga tgacctggtg gagaagttga aggcggcgca ggccgcccag
     6361 cggcaacgca tcgaggcaga agcacgcccc ggtgaatcgt ggcaagcggc cgctgatcga
     6421 atccgcaaag aatcccggca accgccggca gccggtgcgc cgtcgattag gaagccgccc
     6481 aagggcgacg agcaaccaga ttttttcgtt ccgatgctct atgacgtggg cacccgcgat
     6541 agtcgcagca tcatggacgt ggccgttttc cgtctgtcga agcgtgaccg acgagctggc
     6601 gaggtgatcc gctacgagct tccagacggg cacgtagagg tttccgcagg gccggccggc
     6661 atggcgagtg tgtgggatta cgacctggta ctgatggcgg tttcccatct aaccgaatcc
     6721 atgaaccgat accgggaagg gaagggagac aagcccggcc gcgtgttccg tccacacgtt
     6781 gcggacgtac tcaagttctg ccggcgagcc gatggcggaa agcagaaaga cgacctggta
     6841 gaaacctgca ttcggttaaa caccacgcac gttgccatgc agcgtacgaa gaaggccaag
     6901 aacggccgcc tggtgacggt atccgagggt gaagccttga ttagccgcta caagatcgta
     6961 aagagcgaaa ccgggcggcc ggagtacatc gagatcgagc tagctgattg gatgtaccgc
     7021 gagatcacag aaggcaagaa cccggacgtg ctgacggttc accccgatta ctttttgatc
     7081 gatcccggca tcggccgttt tctctaccgc ctggcacgcc gcgccgcagg caaggcagaa
     7141 gccagatggt tgttcaagac gatctacgaa cgcagtggca gcgccggaga gttcaagaag
     7201 ttctgtttca ccgtgcgcaa gctgatcggg tcaaatgacc tgccggagta cgatttgaag
     7261 gaggaggcgg ggcaggctgg cccgatccta gtcatgcgct accgcaacct gatcgagggc
     7321 gaagcatccg ccggttccta atgtacggag cagatgctag ggcaaattgc cctagcaggg
     7381 gaaaaaggtc gaaaaggtct ctttcctgtg gatagcacgt acattgggaa cccaaagccg
     7441 tacattggga accggaaccc gtacattggg aacccaaagc cgtacattgg gaaccggtca
     7501 cacatgtaag tgactgatat aaaagagaaa aaaggcgatt tttccgccta aaactcttta
     7561 aaacttatta aaactcttaa aacccgcctg gcctgtgcat aactgtctgg ccagcgcaca
     7621 gccgaagagc tgcaaaaagc gcctaccctt cggtcgctgc gctccctacg ccccgccgct
     7681 tcgcgtcggc ctatcgcggc cgctggccgc tcaaaaatgg ctggcctacg gccaggcaat
     7741 ctaccagggc gcggacaagc cgcgccgtcg ccactcgacc gccggcgccc acatcaaggc
     7801 accggtgggt atgcctgacg atgcgtggag accgaaacct tgcgctcgtt cgccagccag
     7861 gacagaaatg cctcgacttc gctgctgccc aaggttgccg ggtgacgcac accgtggaaa
     7921 cggatgaagg cacgaaccca gtggacataa gcctgttcgg ttcgtaagct gtaatgcaag
     7981 tagcgtatgc gctcacgcaa ctggtccaga accttgaccg aacgcagcgg tggtaacggc
     8041 gcagtggcgg ttttcatggc ttgttatgac tgtttttttg gggtacagtc tatgcctcgg
     8101 gcatccaagc agcaagcgcg ttacgccgtg ggtcgatgtt tgatgttatg gagcagcaac
     8161 gatgttacgc agcagggcag tcgccctaaa acaaagttaa acatcatgag ggaagcggtg
     8221 atcgccgaag tatcgactca actatcagag gtagttggcg tcatcgagcg ccatctcgaa
     8281 ccgacgttgc tggccgtaca tttgtacggc tccgcagtgg atggcggcct gaagccacac
     8341 agtgatattg atttgctggt tacggtgacc gtaaggcttg atgaaacaac gcggcgagct
     8401 ttgatcaacg accttttgga aacttcggct tcccctggag agagcgagat tctccgcgct
     8461 gtagaagtca ccattgttgt gcacgacgac atcattccgt ggcgttatcc agctaagcgc
     8521 gaactgcaat ttggagaatg gcagcgcaat gacattcttg caggtatctt cgagccagcc
     8581 acgatcgaca ttgatctggc tatcttgctg acaaaagcaa gagaacatag cgttgccttg
     8641 gtaggtccag cggcggagga actctttgat ccggttcctg aacaggatct atttgaggcg
     8701 ctaaatgaaa ccttaacgct atggaactcg ccgcccgact gggctggcga tgagcgaaat
     8761 gtagtgctta cgttgtcccg catttggtac agcgcagtaa ccggcaaaat cgcgccgaag
     8821 gatgtcgctg ccgactgggc aatggagcgc ctgccggccc agtatcagcc cgtcatactt
     8881 gaagctagac aggcttatct tggacaagaa gaagatcgct tggcctcgcg cgcagatcag
     8941 ttggaagaat ttgtccacta cgtgaaaggc gagatcacca aggtagtcgg caaataatgt
     9001 ctaacaattc gttcaagccg acgccgcttc gcggcgcggc ttaactcaag cgttagatgc
     9061 actaagcaca taattgctca cagccaaact atcaggtcaa gtctgctttt attattttta
     9121 agcgtgcata ataagcccta cacaaattgg gagatatatc atgaaaggct ggctttttct
     9181 tgttatcgca atagttggcg aagtaatcgc aacatagctt gcttggtcgt tccgcgtgaa
     9241 cgtcggctcg attgtacctg cgttcaaata ctttgcgatc gtgttgcgcg cctgcccggt
     9301 gcgtcggctg atctcacgga tcgactgctt ctctcgcaac gccatccgac ggatgatgtt
     9361 taaaagtccc atgtggatca ctccgttgcc ccgtcgctca ccgtgttggg gggaaggtgc
     9421 acatggctca gttctcaatg gaaattatct gcctaaccgg ctcagttctg cgtagaaacc
     9481 aacatgcaag ctccaccggg tgcaaagcgg cagcggc
//
