LOCUS       B422p6oN-ocs-LH         9739 bp    DNA     circular SYN 21-JAN-2016
DEFINITION  Ligation of pOCS-BM* into B399p6oN-LH*
ACCESSION   B422p6oN-ocs-LH
KEYWORDS    .
SOURCE      Unknown.
  ORGANISM  Unknown
            Unclassified.
REFERENCE   1  (bases 1 to 9739)
  AUTHORS   Self
  JOURNAL   Unpublished.
COMMENT     SECID/File created by Clone Manager, Scientific & Educational Software
COMMENT     SECNOTES|Vector molecule:  B399p6oN-LH*
               Fragment ends: SfiI
               Fragment size: 9475
            Insert molecule:  pOCS-BM*
               Fragment ends: SfiI
               Fragment size: 264
            Sequencing primers	
            SphR	GGGTGGTTGAACAGAAGTCAT    auch Maxi
FEATURES             Location/Qualifiers
     misc_feature    1..24
                     /gene="BL"
                     /product="left border"
                     /SECDrawAs="Region"
                     /SECStyleId=1
     misc_feature    309..606
                     /gene="Pnos"
                     /product="Promotor"
                     /SECDrawAs="Region"
                     /SECStyleId=1
     CDS             625..1194
                     /gene="hptII"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGR
                     GYVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQD
                     LPETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVY
                     HWQTVMDDTVSASVAQALDELMLWAEDCPE"
                     /SECDrawAs="Gene"
                     /SECStyleId=1
                     /SECName="hptII"
     misc_feature    1195..1383
                     /gene="int"
                     /product="Intron StLS1"
                     /SECDrawAs="Gene"
                     /SECStyleId=1
                     /SECName="int"
                     /SECDescr="Intron StLS1"
     CDS             1384..1839
                     /gene="hptII"
                     /codon_start=3
                     /SECDrawAs="Gene"
                     /SECStyleId=1
     misc_feature    1850..2140
                     /gene="TE9"
                     /product="Terminator Rbcs-E9"
                     /SECDrawAs="Region"
                     /SECStyleId=1
     misc_feature    2236..2431
                     /gene="Tocs"
                     /product="Terminator"
                     /SECDrawAs="Region"
                     /SECStyleId=1
                     /SECName="Tocs"
                     /SECDescr="Terminator"
     misc_feature    2516..2539
                     /gene="BR"
                     /product="right border"
                     /SECDrawAs="Region"
                     /SECStyleId=1
     misc_feature    3074..3771
                     /gene="ColE1"
                     /product="ColE1 ORI"
                     /SECDrawAs="Region"
                     /SECStyleId=1
     misc_feature    4320..8025
                     /gene="pVS1 ORI"
                     /product="pVS1 ORI"
                     /SECDrawAs="Region"
                     /SECStyleId=1
     CDS             8209..9219
                     /gene="Sm/Sp"
                     /product="Strep/Spec resistence"
                     /SECDrawAs="Gene"
                     /SECStyleId=1
ORIGIN      
        1 ggcaggatat attcaattgt aaatggcttc atgtccggga aatctacatg gatcagcaat
       61 gagtatgatg gtcaatatgg agaaaaagaa agagtaatta ccaatttttt ttcaattcaa
      121 aaatgtagat gtccgcagcg ttattataaa atgaaagtac attttgataa aacgacaaat
      181 tacgatccgt cgtatttata ggcgaaagca ataaacaaat tattctaatt cggaaatctt
      241 tatttcgacg tgtctacatt cacgtccaaa tgggggctta gatgagaaac ttcacgatcg
      301 gctctagatc atgagcggag aattaaggga gtcacgttat gacccccgcc gatgacgcgg
      361 gacaagccgt tttacgtttg gaactgacag aaccgcaacg ttgaaggagc cactcagccg
      421 cgggtttctg gagtttaatg agctaagcac atacgtcaga aaccattatt gcgcgttcaa
      481 aagtcgccta aggtcactat cagctagcaa atatttcttg tcaaaaatgc tccactgacg
      541 ttccataaat tcccctcggt atccaattag agtctcatat tcactctcaa tccaaataat
      601 ctgcacctcg agtcctccat aactatgaag aaaccggagc tgaccgccac ttccgtcgaa
      661 aagttcctca tcgagaagtt tgatagcgtg tctgacctta tgcagttgtc ggagggcgaa
      721 gagtcccgcg cgttcagctt tgatgttggc gggcgcgggt acgtcctccg cgtgaactct
      781 tgcgctgacg gcttctataa agatcggtac gtttacaggc atttcgcctc ggcggccctg
      841 cctattcccg aagtcctcga catcggcgag ttttccgaga gccttacgta ttgtatttcc
      901 cgccgggctc aaggggtgac cctccaggat ctgccggaaa ctgagttgcc tgcggttctc
      961 cagcccgtcg ccgaggctat ggacgccatc gccgcggcgg atctttctca aacgtcgggc
     1021 ttcgggccgt tcggccctca ggggattggc cagtacacca cttggaggga ctttatctgc
     1081 gctatcgccg atccgcacgt gtatcattgg caaaccgtta tggacgatac ggtctccgcg
     1141 agcgtggccc aggctctgga cgaactcatg ttgtgggccg aggattgccc cgaggtaagt
     1201 ttctgcttct acctttgata tatatataat aattatcatt aattagtagt aatataatat
     1261 ttcaaatatt tttttcaaaa taaaagaatg tagtatatag caattgcttt tctgtagttt
     1321 ataagtgtgt atattttaat ttataacttt tctaatatat gaccaaaatt tgttgatgtg
     1381 caggtccgcc acctggttca tgcggacttc ggctctaata acgtgctcac cgataatggg
     1441 cggattactg ccgtcatcga ctggtccgaa gctatgtttg gcgattcgca gtacgaagtg
     1501 gcgaacattt tcttctggag gccttggctt gcctgcatgg aacagcaaac gcgctacttt
     1561 gagcggaggc acccggagct ggcggggtct ccccgcctca gggcctatat gttgcggatc
     1621 ggcctcgacc agctttacca gtccctggtt gatgggaatt tcgacgatgc tgcctgggcg
     1681 caaggccgct gtgacgctat tgtcaggagc ggcgcgggga ccgtgggccg cactcagatc
     1741 gcccggcggt cggctgccgt ttggacggat gggtgcgtcg aagtgctcgc ggacagcggc
     1801 aacaggcggc cttccacccg cccgcgggcc aaggagtgaa cgcgttcgag tattatggca
     1861 ttgggaaaac tgtttttctt gtaccatttg ttgtgcttgt aatttactgt gttttttatt
     1921 cggttttcgc tatcgaactg tgaaatggaa atggatggag aagagttaat gaatgatatg
     1981 gtccttttgt tcattctcaa attaatatta tttgtttttt ctcttatttg ttgtgtgttg
     2041 aatttgaaat tataagagat atgcaaacat tttgttttga gtaaaaatgt gtcaaatcgt
     2101 ggcctctaat gaccgaagtt aatatgagga gtaaaacact gaagcctgca ggcatgcaag
     2161 ctgatccact agaggccatg gcggccgcac tagtggatcc cccgggctgc aggaattcaa
     2221 gcttacgcgt gtcgaccctg ctttaatgag atatgcgaga cgcctatgat cgcatgatat
     2281 ttgctttcaa ttctgttgtg cacgttgtaa aaaacctgag catgtgtagc tcagatcctt
     2341 accgccggtt tcggttcatt ctaatgaata tatcacccgt tactatcgta tttttatgaa
     2401 taatattctc cgttcaattt actgattgtc ctcgacaggc cttaagggcc agatcttggg
     2461 cccggtaccc gatcagattg tcgtttcccg ccttcggttt aaactatcag tgtttgacag
     2521 gatatattgg cgggtaaacc taagagaaaa gagcgtttat tagaataatc ggatatttaa
     2581 aagggcgtga aaaggtttat ccgttcgtcc atttgtatgt gcatgccaac cacagggttc
     2641 ccctcgggag tgcttggcat tccgtgcgat aatgacttct gttcaaccac ccaaacgtcg
     2701 gaaagcctga cgacggagca gcattccaaa aagatccctt ggctcgtctg ggtcggctag
     2761 aaggtcgagt gggctgctgt ggcttgatcc ctcaacgcgg tcgcggacgt agcgcagcgc
     2821 cgaaaaatcc tcgatcgcaa atccgacgct gtcgaaaagc gtgatctgct tgtcgctctt
     2881 tcggccgacg tcctggccag tcatcacgcg ccaaagttcc gtcacaggat gatctggcgc
     2941 gagttgctgg atctcgcctt caatccgggt ctgtggcggg aactccacga aaatatccga
     3001 acgcagcaag atatcgcggt gcatctcggt cttgcctggg cagtcgccgc cgacgccgtt
     3061 gatgtggacg ccgaaaagga tctaggtgaa gatccttttt gataatctca tgaccaaaat
     3121 cccttaacgt gagttttcgt tccactgagc gtcagacccc gtagaaaaga tcaaaggatc
     3181 ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct
     3241 accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga aggtaactgg
     3301 cttcagcaga gcgcagatac caaatactgt tcttctagtg tagccgtagt taggccacca
     3361 cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt taccagtggc
     3421 tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat agttaccgga
     3481 taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct tggagcgaac
     3541 gacctacacc gaactgagat acctacagcg tgagctatga gaaagcgcca cgcttcccga
     3601 agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag agcgcacgag
     3661 ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc gccacctctg
     3721 acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga aaaacgccag
     3781 caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca tgttctttcc
     3841 tgcgttatcc cctgattctg tggataaccg attaccgcct ttgagtgagc tgataccgct
     3901 cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg aggaagcgga agagcgcctg
     3961 atgcggtatt ttctccttac gcatctgtgc ggtatttcac accgcatatg gtgcactctc
     4021 agtacaatct gctctgatgc cgcatagtta agccagtata cactccgcta tcgctacgtg
     4081 actgggtcat ggctgcgccc cgacacccgc caacacccgc tgacgcgccc tgacgggctt
     4141 gtctgctccc ggcatccgct tacagacaag ctgtgaccgt ctccgggagc tgcatgtgtc
     4201 agaggttttc accgtcatca ccgaaacgcg cgaggcaggg gtacgtcgag gtcgatccaa
     4261 cccctccgct gctatagtgc agtcggcttc tgacgttcag tgcagccgtc ttctgaaaac
     4321 gacatgtcgc acaagtccta agttacgcga caggctgccg ccctgccctt ttcctggcgt
     4381 tttcttgtcg cgtgttttag tcgcataaag tagaatactt gcgactagaa ccggagacat
     4441 tacgccatga acaagagcgc cgccgctggc ctgctgggct atgcccgcgt cagcaccgac
     4501 gaccaggact tgaccaacca acgggccgaa ctgcacgcgg ccggctgcac caagctgttt
     4561 tccgagaaga tcaccggcac caggcgcgac cgcccggagc tggccaggat gcttgaccac
     4621 ctacgccctg gcgacgttgt gacagtgacc aggctagacc gcctggcccg cagcacccgc
     4681 gacctactgg acattgccga gcgcatccag gaggccggcg cgggcctgcg tagcctggca
     4741 gagccgtggg ccgacaccac cacgccggcc ggccgcatgg tgttgaccgt gttcgccggc
     4801 attgccgagt tcgagcgttc cctaatcatc gaccgcaccc ggagcgggcg cgaggccgcc
     4861 aaggcgcgag gcgtgaagtt tggcccccgc cctaccctca ccccggcaca gatcgcgcac
     4921 gcccgcgagc tgatcgacca ggaaggccgc accgtgaaag aggcggctgc actgcttggc
     4981 gtgcatcgct cgaccctgta ccgcgcactt gagcgcagcg aggaagtgac gcccaccgag
     5041 gccaggcggc gcggtgcctt ccgtgaggac gcattgaccg aggccgacgc cctggcggcc
     5101 gccgagaatg aacgccaaga ggaacaagca tgaaaccgca ccaggacggc caggacgaac
     5161 cgtttttcat taccgaagag atcgaggcgg agatgatcgc ggccgggtac gtgttcgagc
     5221 cgcccgcgca cgtctcaacc gtgcggctgc atgaaatcct ggccggtttg tctgatgcca
     5281 agctcgcggc ctggccggcg agcttggccg ctgaagaaac cgagcgccgc cgtctaaaaa
     5341 ggtgatgtgt atttgagtaa aacagcttgc gtcatgcggt cgctgcgtat atgatgcgat
     5401 gagtaaataa acaaatacgc aaggggaacg catgaaggtt atcgctgtac ttaaccagaa
     5461 aggcgggtca ggcaagacga ccatcgcaac ccatctagcc cgcgccctgc aactcgccgg
     5521 ggccgatgtt ctgttagtcg attccgatcc ccagggcagt gcccgcgatt gggcggccgt
     5581 gcgggaagat caaccgctaa ccgttgtcgg catcgaccgc ccgacgattg accgcgacgt
     5641 gaaggccatc ggccggcgcg acttcgtagt gatcgacgga gcgccccagg cggcggactt
     5701 ggctgtgtcc gcgatcaagg cagccgactt cgtgctgatt ccggtgcagc caagccctta
     5761 cgacatatgg gccaccgccg acctggtgga gctggttaag cagcgcattg aggtcacgga
     5821 tggaaggcta caagcggcct ttgtcgtgtc gcgggcgatc aaaggcacgc gcatcggcgg
     5881 tgaggttgcc gaggcgctgg ccgggtacga gctgcccatt cttgagtccc gtatcacgca
     5941 gcgcgtgagc tacccaggca ctgccgccgc cggcacaacc gttcttgaat cagaacccga
     6001 gggcgacgct gcccgcgagg tccaggcgct ggccgctgaa attaaatcaa aactcatttg
     6061 agttaatgag gtaaagagaa aatgagcaaa agcacaaaca cgctaagtgc cggccgtccg
     6121 agcgcacgca gcagcaaggc tgcaacgttg gccagcctgg cagacacgcc agccatgaag
     6181 cgggtcaact ttcagttgcc ggcggaggat cacaccaagc tgaagatgta cgcggtacgc
     6241 caaggcaaga ccattaccga gctgctatct gaatacatcg cgcagctacc agagtaaatg
     6301 agcaaatgaa taaatgagta gatgaatttt agcggctaaa ggaggcggca tggaaaatca
     6361 agaacaacca ggcaccgacg ccgtggaatg ccccatgtgt ggaggaacgg gcggttggcc
     6421 aggcgtaagc ggctgggttg tctgccggcc ctgcaatggc actggaaccc ccaagcccga
     6481 ggaatcggcg tgagcggtcg caaaccatcc ggcccggtac aaatcggcgc ggcgctgggt
     6541 gatgacctgg tggagaagtt gaaggcggcg caggccgccc agcggcaacg catcgaggca
     6601 gaagcacgcc ccggtgaatc gtggcaagcg gccgctgatc gaatccgcaa agaatcccgg
     6661 caaccgccgg cagccggtgc gccgtcgatt aggaagccgc ccaagggcga cgagcaacca
     6721 gattttttcg ttccgatgct ctatgacgtg ggcacccgcg atagtcgcag catcatggac
     6781 gtggccgttt tccgtctgtc gaagcgtgac cgacgagctg gcgaggtgat ccgctacgag
     6841 cttccagacg ggcacgtaga ggtttccgca gggccggccg gcatggcgag tgtgtgggat
     6901 tacgacctgg tactgatggc ggtttcccat ctaaccgaat ccatgaaccg ataccgggaa
     6961 gggaagggag acaagcccgg ccgcgtgttc cgtccacacg ttgcggacgt actcaagttc
     7021 tgccggcgag ccgatggcgg aaagcagaaa gacgacctgg tagaaacctg cattcggtta
     7081 aacaccacgc acgttgccat gcagcgtacg aagaaggcca agaacggccg cctggtgacg
     7141 gtatccgagg gtgaagcctt gattagccgc tacaagatcg taaagagcga aaccgggcgg
     7201 ccggagtaca tcgagatcga gctagctgat tggatgtacc gcgagatcac agaaggcaag
     7261 aacccggacg tgctgacggt tcaccccgat tactttttga tcgatcccgg catcggccgt
     7321 tttctctacc gcctggcacg ccgcgccgca ggcaaggcag aagccagatg gttgttcaag
     7381 acgatctacg aacgcagtgg cagcgccgga gagttcaaga agttctgttt caccgtgcgc
     7441 aagctgatcg ggtcaaatga cctgccggag tacgatttga aggaggaggc ggggcaggct
     7501 ggcccgatcc tagtcatgcg ctaccgcaac ctgatcgagg gcgaagcatc cgccggttcc
     7561 taatgtacgg agcagatgct agggcaaatt gccctagcag gggaaaaagg tcgaaaaggt
     7621 ctctttcctg tggatagcac gtacattggg aacccaaagc cgtacattgg gaaccggaac
     7681 ccgtacattg ggaacccaaa gccgtacatt gggaaccggt cacacatgta agtgactgat
     7741 ataaaagaga aaaaaggcga tttttccgcc taaaactctt taaaacttat taaaactctt
     7801 aaaacccgcc tggcctgtgc ataactgtct ggccagcgca cagccgaaga gctgcaaaaa
     7861 gcgcctaccc ttcggtcgct gcgctcccta cgccccgccg cttcgcgtcg gcctatcgcg
     7921 gccgctggcc gctcaaaaat ggctggccta cggccaggca atctaccagg gcgcggacaa
     7981 gccgcgccgt cgccactcga ccgccggcgc ccacatcaag gcaccggtgg gtatgcctga
     8041 cgatgcgtgg agaccgaaac cttgcgctcg ttcgccagcc aggacagaaa tgcctcgact
     8101 tcgctgctgc ccaaggttgc cgggtgacgc acaccgtgga aacggatgaa ggcacgaacc
     8161 cagtggacat aagcctgttc ggttcgtaag ctgtaatgca agtagcgtat gcgctcacgc
     8221 aactggtcca gaaccttgac cgaacgcagc ggtggtaacg gcgcagtggc ggttttcatg
     8281 gcttgttatg actgtttttt tggggtacag tctatgcctc gggcatccaa gcagcaagcg
     8341 cgttacgccg tgggtcgatg tttgatgtta tggagcagca acgatgttac gcagcagggc
     8401 agtcgcccta aaacaaagtt aaacatcatg agggaagcgg tgatcgccga agtatcgact
     8461 caactatcag aggtagttgg cgtcatcgag cgccatctcg aaccgacgtt gctggccgta
     8521 catttgtacg gctccgcagt ggatggcggc ctgaagccac acagtgatat tgatttgctg
     8581 gttacggtga ccgtaaggct tgatgaaaca acgcggcgag ctttgatcaa cgaccttttg
     8641 gaaacttcgg cttcccctgg agagagcgag attctccgcg ctgtagaagt caccattgtt
     8701 gtgcacgacg acatcattcc gtggcgttat ccagctaagc gcgaactgca atttggagaa
     8761 tggcagcgca atgacattct tgcaggtatc ttcgagccag ccacgatcga cattgatctg
     8821 gctatcttgc tgacaaaagc aagagaacat agcgttgcct tggtaggtcc agcggcggag
     8881 gaactctttg atccggttcc tgaacaggat ctatttgagg cgctaaatga aaccttaacg
     8941 ctatggaact cgccgcccga ctgggctggc gatgagcgaa atgtagtgct tacgttgtcc
     9001 cgcatttggt acagcgcagt aaccggcaaa atcgcgccga aggatgtcgc tgccgactgg
     9061 gcaatggagc gcctgccggc ccagtatcag cccgtcatac ttgaagctag acaggcttat
     9121 cttggacaag aagaagatcg cttggcctcg cgcgcagatc agttggaaga atttgtccac
     9181 tacgtgaaag gcgagatcac caaggtagtc ggcaaataat gtctaacaat tcgttcaagc
     9241 cgacgccgct tcgcggcgcg gcttaactca agcgttagat gcactaagca cataattgct
     9301 cacagccaaa ctatcaggtc aagtctgctt ttattatttt taagcgtgca taataagccc
     9361 tacacaaatt gggagatata tcatgaaagg ctggcttttt cttgttatcg caatagttgg
     9421 cgaagtaatc gcaacatagc ttgcttggtc gttccgcgtg aacgtcggct cgattgtacc
     9481 tgcgttcaaa tactttgcga tcgtgttgcg cgcctgcccg gtgcgtcggc tgatctcacg
     9541 gatcgactgc ttctctcgca acgccatccg acggatgatg tttaaaagtc ccatgtggat
     9601 cactccgttg ccccgtcgct caccgtgttg gggggaaggt gcacatggct cagttctcaa
     9661 tggaaattat ctgcctaacc ggctcagttc tgcgtagaaa ccaacatgca agctccaccg
     9721 ggtgcaaagc ggcagcggc
//
