LOCUS B413p6o-LH 9659 bp DNA circular SYN 21-JAN-2016 DEFINITION Ligation of B399p6oN-LH* into pLH7000M* ACCESSION B413p6o-LH KEYWORDS . SOURCE Unknown. ORGANISM Unknown Unclassified. REFERENCE 1 (bases 1 to 9659) AUTHORS Self JOURNAL Unpublished. COMMENT SECID/File created by Clone Manager, Scientific & Educational Software COMMENT SECNOTES|Vector molecule: pLH7000M* Fragment ends: PstI and XhoI Fragment size: 8116 Insert molecule: B399p6oN-LH* Fragment ends: XhoI and PstI Fragment size: 1543 Sequencing primers 35S GCACAATCCCACTATCCTT zeigt Unterschied Mini und Maxi LH1.2 CGAAGGCGGGAAACGACAAT nur Maxi FEATURES Location/Qualifiers misc_feature 1..24 /gene="LB" /product="Left Border" /SECDrawAs="Region" /SECStyleId=1 misc_feature 309..724 /gene="P35S" /product="35S-Promotor" /SECDrawAs="Region" /SECStyleId=1 CDS 745..1314 /gene="hptII" /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGR GYVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQD LPETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVY HWQTVMDDTVSASVAQALDELMLWAEDCPE" /SECDrawAs="Gene" /SECStyleId=1 /SECName="hptII" misc_feature 1315..1503 /gene="int" /product="Intron StLS1" /SECDrawAs="Gene" /SECStyleId=1 /SECName="int" /SECDescr="Intron StLS1" CDS 1504..1959 /gene="hptII" /codon_start=3 /SECDrawAs="Gene" /SECStyleId=1 misc_feature 1970..2260 /gene="TE9" /product="Terminator Rbcs-E9" /SECDrawAs="Region" /SECStyleId=1 misc_feature 2436..2459 /gene="RB" /product="Right Border" /SECDrawAs="Region" /SECStyleId=1 misc_feature 2994..3691 /gene="ColE1" /product="ColE1 ORI" /SECDrawAs="Region" /SECStyleId=1 misc_feature 3728..3987 /gene="bom" /SECDrawAs="Region" /SECStyleId=1 misc_feature 4240..7945 /gene="pVS1" /product="ori for agrobacteria" /SECDrawAs="Region" /SECStyleId=1 CDS 8129..9139 /gene="Sm/Sp" /product="Strep/Spec resistence" /SECDrawAs="Gene" /SECStyleId=1 ORIGIN 1 ggcaggatat attcaattgt aaatggcttc atgtccggga aatctacatg gatcagcaat 61 gagtatgatg gtcaatatgg agaaaaagaa agagtaatta ccaatttttt ttcaattcaa 121 aaatgtagat gtccgcagcg ttattataaa atgaaagtac attttgataa aacgacaaat 181 tacgatccgt cgtatttata ggcgaaagca ataaacaaat tattctaatt cggaaatctt 241 tatttcgacg tgtctacatt cacgtccaaa tgggggctta gatgagaaac ttcacgatcg 301 gctctagaac atggtggagc acgacactct cgtctactcc aagaatatca aagatacagt 361 ctcagaagac cagagggcta ttgagacttt tcaacaaagg gtaatatcgg gaaacctcct 421 cggattccat tgcccagcta tctgtcactt catcgaaagg acagtagaaa aggaagatgg 481 cttctacaaa tgccatcatt gcgataaagg aaaggctatc gttcaagatg cctctaccga 541 cagtggtccc aaagatggac ccccacccac gaggaacatc gtggaaaaag aagacgttcc 601 aaccacgtct tcaaagcaag tggattgatg tgatatctcc actgacgtaa gggatgacgc 661 acaatcccac tatccttcgc aagacccttc ctctatataa ggaagttcat ttcatttgga 721 gaggacctcg agtcctccat aactatgaag aaaccggagc tgaccgccac ttccgtcgaa 781 aagttcctca tcgagaagtt tgatagcgtg tctgacctta tgcagttgtc ggagggcgaa 841 gagtcccgcg cgttcagctt tgatgttggc gggcgcgggt acgtcctccg cgtgaactct 901 tgcgctgacg gcttctataa agatcggtac gtttacaggc atttcgcctc ggcggccctg 961 cctattcccg aagtcctcga catcggcgag ttttccgaga gccttacgta ttgtatttcc 1021 cgccgggctc aaggggtgac cctccaggat ctgccggaaa ctgagttgcc tgcggttctc 1081 cagcccgtcg ccgaggctat ggacgccatc gccgcggcgg atctttctca aacgtcgggc 1141 ttcgggccgt tcggccctca ggggattggc cagtacacca cttggaggga ctttatctgc 1201 gctatcgccg atccgcacgt gtatcattgg caaaccgtta tggacgatac ggtctccgcg 1261 agcgtggccc aggctctgga cgaactcatg ttgtgggccg aggattgccc cgaggtaagt 1321 ttctgcttct acctttgata tatatataat aattatcatt aattagtagt aatataatat 1381 ttcaaatatt tttttcaaaa taaaagaatg tagtatatag caattgcttt tctgtagttt 1441 ataagtgtgt atattttaat ttataacttt tctaatatat gaccaaaatt tgttgatgtg 1501 caggtccgcc acctggttca tgcggacttc ggctctaata acgtgctcac cgataatggg 1561 cggattactg ccgtcatcga ctggtccgaa gctatgtttg gcgattcgca gtacgaagtg 1621 gcgaacattt tcttctggag gccttggctt gcctgcatgg aacagcaaac gcgctacttt 1681 gagcggaggc acccggagct ggcggggtct ccccgcctca gggcctatat gttgcggatc 1741 ggcctcgacc agctttacca gtccctggtt gatgggaatt tcgacgatgc tgcctgggcg 1801 caaggccgct gtgacgctat tgtcaggagc ggcgcgggga ccgtgggccg cactcagatc 1861 gcccggcggt cggctgccgt ttggacggat gggtgcgtcg aagtgctcgc ggacagcggc 1921 aacaggcggc cttccacccg cccgcgggcc aaggagtgaa cgcgttcgag tattatggca 1981 ttgggaaaac tgtttttctt gtaccatttg ttgtgcttgt aatttactgt gttttttatt 2041 cggttttcgc tatcgaactg tgaaatggaa atggatggag aagagttaat gaatgatatg 2101 gtccttttgt tcattctcaa attaatatta tttgtttttt ctcttatttg ttgtgtgttg 2161 aatttgaaat tataagagat atgcaaacat tttgttttga gtaaaaatgt gtcaaatcgt 2221 ggcctctaat gaccgaagtt aatatgagga gtaaaacact gaagcctgca ggcatgcaag 2281 ctgatccact agaggccatg gcggccgcac tagtggatcc cccgggatgc atgaattcaa 2341 gcttatcgat gtcgacaggc cttaagggcc agatcttggg cccggtaccc gatcagattg 2401 tcgtttcccg ccttcggttt aaactatcag tgtttgacag gatatattgg cgggtaaacc 2461 taagagaaaa gagcgtttat tagaataatc ggatatttaa aagggcgtga aaaggtttat 2521 ccgttcgtcc atttgtatgt gcatgccaac cacagggttc ccctcgggag tgcttggcat 2581 tccgtgcgat aatgacttct gttcaaccac ccaaacgtcg gaaagcctga cgacggagca 2641 gcattccaaa aagatccctt ggctcgtctg ggtcggctag aaggtcgagt gggctgctgt 2701 ggcttgatcc ctcaacgcgg tcgcggacgt agcgcagcgc cgaaaaatcc tcgatcgcaa 2761 atccgacgct gtcgaaaagc gtgatctgct tgtcgctctt tcggccgacg tcctggccag 2821 tcatcacgcg ccaaagttcc gtcacaggat gatctggcgc gagttgctgg atctcgcctt 2881 caatccgggt ctgtggcggg aactccacga aaatatccga acgcagcaag atatcgcggt 2941 gcatctcggt cttgcctggg cagtcgccgc cgacgccgtt gatgtggacg ccgaaaagga 3001 tctaggtgaa gatccttttt gataatctca tgaccaaaat cccttaacgt gagttttcgt 3061 tccactgagc gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat cctttttttc 3121 tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct accagcggtg gtttgtttgc 3181 cggatcaaga gctaccaact ctttttccga aggtaactgg cttcagcaga gcgcagatac 3241 caaatactgt tcttctagtg tagccgtagt taggccacca cttcaagaac tctgtagcac 3301 cgcctacata cctcgctctg ctaatcctgt taccagtggc tgctgccagt ggcgataagt 3361 cgtgtcttac cgggttggac tcaagacgat agttaccgga taaggcgcag cggtcgggct 3421 gaacgggggg ttcgtgcaca cagcccagct tggagcgaac gacctacacc gaactgagat 3481 acctacagcg tgagctatga gaaagcgcca cgcttcccga agggagaaag gcggacaggt 3541 atccggtaag cggcagggtc ggaacaggag agcgcacgag ggagcttcca gggggaaacg 3601 cctggtatct ttatagtcct gtcgggtttc gccacctctg acttgagcgt cgatttttgt 3661 gatgctcgtc aggggggcgg agcctatgga aaaacgccag caacgcggcc tttttacggt 3721 tcctggcctt ttgctggcct tttgctcaca tgttctttcc tgcgttatcc cctgattctg 3781 tggataaccg attaccgcct ttgagtgagc tgataccgct cgccgcagcc gaacgaccga 3841 gcgcagcgag tcagtgagcg aggaagcgga agagcgcctg atgcggtatt ttctccttac 3901 gcatctgtgc ggtatttcac accgcatatg gtgcactctc agtacaatct gctctgatgc 3961 cgcatagtta agccagtata cactccgcta tcgctacgtg actgggtcat ggctgcgccc 4021 cgacacccgc caacacccgc tgacgcgccc tgacgggctt gtctgctccc ggcatccgct 4081 tacagacaag ctgtgaccgt ctccgggagc tgcatgtgtc agaggttttc accgtcatca 4141 ccgaaacgcg cgaggcaggg gtacgtcgag gtcgatccaa cccctccgct gctatagtgc 4201 agtcggcttc tgacgttcag tgcagccgtc ttctgaaaac gacatgtcgc acaagtccta 4261 agttacgcga caggctgccg ccctgccctt ttcctggcgt tttcttgtcg cgtgttttag 4321 tcgcataaag tagaatactt gcgactagaa ccggagacat tacgccatga acaagagcgc 4381 cgccgctggc ctgctgggct atgcccgcgt cagcaccgac gaccaggact tgaccaacca 4441 acgggccgaa ctgcacgcgg ccggctgcac caagctgttt tccgagaaga tcaccggcac 4501 caggcgcgac cgcccggagc tggccaggat gcttgaccac ctacgccctg gcgacgttgt 4561 gacagtgacc aggctagacc gcctggcccg cagcacccgc gacctactgg acattgccga 4621 gcgcatccag gaggccggcg cgggcctgcg tagcctggca gagccgtggg ccgacaccac 4681 cacgccggcc ggccgcatgg tgttgaccgt gttcgccggc attgccgagt tcgagcgttc 4741 cctaatcatc gaccgcaccc ggagcgggcg cgaggccgcc aaggcgcgag gcgtgaagtt 4801 tggcccccgc cctaccctca ccccggcaca gatcgcgcac gcccgcgagc tgatcgacca 4861 ggaaggccgc accgtgaaag aggcggctgc actgcttggc gtgcatcgct cgaccctgta 4921 ccgcgcactt gagcgcagcg aggaagtgac gcccaccgag gccaggcggc gcggtgcctt 4981 ccgtgaggac gcattgaccg aggccgacgc cctggcggcc gccgagaatg aacgccaaga 5041 ggaacaagca tgaaaccgca ccaggacggc caggacgaac cgtttttcat taccgaagag 5101 atcgaggcgg agatgatcgc ggccgggtac gtgttcgagc cgcccgcgca cgtctcaacc 5161 gtgcggctgc atgaaatcct ggccggtttg tctgatgcca agctcgcggc ctggccggcg 5221 agcttggccg ctgaagaaac cgagcgccgc cgtctaaaaa ggtgatgtgt atttgagtaa 5281 aacagcttgc gtcatgcggt cgctgcgtat atgatgcgat gagtaaataa acaaatacgc 5341 aaggggaacg catgaaggtt atcgctgtac ttaaccagaa aggcgggtca ggcaagacga 5401 ccatcgcaac ccatctagcc cgcgccctgc aactcgccgg ggccgatgtt ctgttagtcg 5461 attccgatcc ccagggcagt gcccgcgatt gggcggccgt gcgggaagat caaccgctaa 5521 ccgttgtcgg catcgaccgc ccgacgattg accgcgacgt gaaggccatc ggccggcgcg 5581 acttcgtagt gatcgacgga gcgccccagg cggcggactt ggctgtgtcc gcgatcaagg 5641 cagccgactt cgtgctgatt ccggtgcagc caagccctta cgacatatgg gccaccgccg 5701 acctggtgga gctggttaag cagcgcattg aggtcacgga tggaaggcta caagcggcct 5761 ttgtcgtgtc gcgggcgatc aaaggcacgc gcatcggcgg tgaggttgcc gaggcgctgg 5821 ccgggtacga gctgcccatt cttgagtccc gtatcacgca gcgcgtgagc tacccaggca 5881 ctgccgccgc cggcacaacc gttcttgaat cagaacccga gggcgacgct gcccgcgagg 5941 tccaggcgct ggccgctgaa attaaatcaa aactcatttg agttaatgag gtaaagagaa 6001 aatgagcaaa agcacaaaca cgctaagtgc cggccgtccg agcgcacgca gcagcaaggc 6061 tgcaacgttg gccagcctgg cagacacgcc agccatgaag cgggtcaact ttcagttgcc 6121 ggcggaggat cacaccaagc tgaagatgta cgcggtacgc caaggcaaga ccattaccga 6181 gctgctatct gaatacatcg cgcagctacc agagtaaatg agcaaatgaa taaatgagta 6241 gatgaatttt agcggctaaa ggaggcggca tggaaaatca agaacaacca ggcaccgacg 6301 ccgtggaatg ccccatgtgt ggaggaacgg gcggttggcc aggcgtaagc ggctgggttg 6361 tctgccggcc ctgcaatggc actggaaccc ccaagcccga ggaatcggcg tgagcggtcg 6421 caaaccatcc ggcccggtac aaatcggcgc ggcgctgggt gatgacctgg tggagaagtt 6481 gaaggcggcg caggccgccc agcggcaacg catcgaggca gaagcacgcc ccggtgaatc 6541 gtggcaagcg gccgctgatc gaatccgcaa agaatcccgg caaccgccgg cagccggtgc 6601 gccgtcgatt aggaagccgc ccaagggcga cgagcaacca gattttttcg ttccgatgct 6661 ctatgacgtg ggcacccgcg atagtcgcag catcatggac gtggccgttt tccgtctgtc 6721 gaagcgtgac cgacgagctg gcgaggtgat ccgctacgag cttccagacg ggcacgtaga 6781 ggtttccgca gggccggccg gcatggcgag tgtgtgggat tacgacctgg tactgatggc 6841 ggtttcccat ctaaccgaat ccatgaaccg ataccgggaa gggaagggag acaagcccgg 6901 ccgcgtgttc cgtccacacg ttgcggacgt actcaagttc tgccggcgag ccgatggcgg 6961 aaagcagaaa gacgacctgg tagaaacctg cattcggtta aacaccacgc acgttgccat 7021 gcagcgtacg aagaaggcca agaacggccg cctggtgacg gtatccgagg gtgaagcctt 7081 gattagccgc tacaagatcg taaagagcga aaccgggcgg ccggagtaca tcgagatcga 7141 gctagctgat tggatgtacc gcgagatcac agaaggcaag aacccggacg tgctgacggt 7201 tcaccccgat tactttttga tcgatcccgg catcggccgt tttctctacc gcctggcacg 7261 ccgcgccgca ggcaaggcag aagccagatg gttgttcaag acgatctacg aacgcagtgg 7321 cagcgccgga gagttcaaga agttctgttt caccgtgcgc aagctgatcg ggtcaaatga 7381 cctgccggag tacgatttga aggaggaggc ggggcaggct ggcccgatcc tagtcatgcg 7441 ctaccgcaac ctgatcgagg gcgaagcatc cgccggttcc taatgtacgg agcagatgct 7501 agggcaaatt gccctagcag gggaaaaagg tcgaaaaggt ctctttcctg tggatagcac 7561 gtacattggg aacccaaagc cgtacattgg gaaccggaac ccgtacattg ggaacccaaa 7621 gccgtacatt gggaaccggt cacacatgta agtgactgat ataaaagaga aaaaaggcga 7681 tttttccgcc taaaactctt taaaacttat taaaactctt aaaacccgcc tggcctgtgc 7741 ataactgtct ggccagcgca cagccgaaga gctgcaaaaa gcgcctaccc ttcggtcgct 7801 gcgctcccta cgccccgccg cttcgcgtcg gcctatcgcg gccgctggcc gctcaaaaat 7861 ggctggccta cggccaggca atctaccagg gcgcggacaa gccgcgccgt cgccactcga 7921 ccgccggcgc ccacatcaag gcaccggtgg gtatgcctga cgatgcgtgg agaccgaaac 7981 cttgcgctcg ttcgccagcc aggacagaaa tgcctcgact tcgctgctgc ccaaggttgc 8041 cgggtgacgc acaccgtgga aacggatgaa ggcacgaacc cagtggacat aagcctgttc 8101 ggttcgtaag ctgtaatgca agtagcgtat gcgctcacgc aactggtcca gaaccttgac 8161 cgaacgcagc ggtggtaacg gcgcagtggc ggttttcatg gcttgttatg actgtttttt 8221 tggggtacag tctatgcctc gggcatccaa gcagcaagcg cgttacgccg tgggtcgatg 8281 tttgatgtta tggagcagca acgatgttac gcagcagggc agtcgcccta aaacaaagtt 8341 aaacatcatg agggaagcgg tgatcgccga agtatcgact caactatcag aggtagttgg 8401 cgtcatcgag cgccatctcg aaccgacgtt gctggccgta catttgtacg gctccgcagt 8461 ggatggcggc ctgaagccac acagtgatat tgatttgctg gttacggtga ccgtaaggct 8521 tgatgaaaca acgcggcgag ctttgatcaa cgaccttttg gaaacttcgg cttcccctgg 8581 agagagcgag attctccgcg ctgtagaagt caccattgtt gtgcacgacg acatcattcc 8641 gtggcgttat ccagctaagc gcgaactgca atttggagaa tggcagcgca atgacattct 8701 tgcaggtatc ttcgagccag ccacgatcga cattgatctg gctatcttgc tgacaaaagc 8761 aagagaacat agcgttgcct tggtaggtcc agcggcggag gaactctttg atccggttcc 8821 tgaacaggat ctatttgagg cgctaaatga aaccttaacg ctatggaact cgccgcccga 8881 ctgggctggc gatgagcgaa atgtagtgct tacgttgtcc cgcatttggt acagcgcagt 8941 aaccggcaaa atcgcgccga aggatgtcgc tgccgactgg gcaatggagc gcctgccggc 9001 ccagtatcag cccgtcatac ttgaagctag acaggcttat cttggacaag aagaagatcg 9061 cttggcctcg cgcgcagatc agttggaaga atttgtccac tacgtgaaag gcgagatcac 9121 caaggtagtc ggcaaataat gtctaacaat tcgttcaagc cgacgccgct tcgcggcgcg 9181 gcttaactca agcgttagat gcactaagca cataattgct cacagccaaa ctatcaggtc 9241 aagtctgctt ttattatttt taagcgtgca taataagccc tacacaaatt gggagatata 9301 tcatgaaagg ctggcttttt cttgttatcg caatagttgg cgaagtaatc gcaacatagc 9361 ttgcttggtc gttccgcgtg aacgtcggct cgattgtacc tgcgttcaaa tactttgcga 9421 tcgtgttgcg cgcctgcccg gtgcgtcggc tgatctcacg gatcgactgc ttctctcgca 9481 acgccatccg acggatgatg tttaaaagtc ccatgtggat cactccgttg ccccgtcgct 9541 caccgtgttg gggggaaggt gcacatggct cagttctcaa tggaaattat ctgcctaacc 9601 ggctcagttc tgcgtagaaa ccaacatgca agctccaccg ggtgcaaagc ggcagcggc //