LOCUS       B423p6o-LH-35s-o       10287 bp    DNA     circular SYN 21-JAN-2016
DEFINITION  Ligation of p35-OCS-BM* into B413p6o-LH*
ACCESSION   B423p6o-LH-35s-o
KEYWORDS    .
SOURCE      Unknown.
  ORGANISM  Unknown
            Unclassified.
REFERENCE   1  (bases 1 to 10287)
  AUTHORS   Self
  JOURNAL   Unpublished.
COMMENT     SECID/File created by Clone Manager, Scientific & Educational Software
COMMENT     SECNOTES|Vector molecule:  B413p6o-LH*
               Fragment ends: SfiI
               Fragment size: 9595
            Insert molecule:  p35-OCS-BM*
               Fragment ends: SfiI
               Fragment size: 692
            Sequencing primers	
            E9F	GTGTCAAATCGTGGCCTCTAA
FEATURES             Location/Qualifiers
     misc_feature    1..24
                     /gene="LB"
                     /product="Left Border"
                     /SECDrawAs="Region"
                     /SECStyleId=1
     misc_feature    309..724
                     /gene="P35S"
                     /product="35S-Promotor"
                     /SECDrawAs="Region"
                     /SECStyleId=1
     CDS             745..1314
                     /gene="hptII"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGR
                     GYVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQD
                     LPETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVY
                     HWQTVMDDTVSASVAQALDELMLWAEDCPE"
                     /SECDrawAs="Gene"
                     /SECStyleId=1
                     /SECName="hptII"
     misc_feature    1315..1503
                     /gene="int"
                     /product="Intron StLS1"
                     /SECDrawAs="Gene"
                     /SECStyleId=1
                     /SECName="int"
                     /SECDescr="Intron StLS1"
     CDS             1504..1959
                     /gene="hptII"
                     /codon_start=3
                     /SECDrawAs="Gene"
                     /SECStyleId=1
     misc_feature    1970..2260
                     /gene="TE9"
                     /product="Terminator Rbcs-E9"
                     /SECDrawAs="Region"
                     /SECStyleId=1
     misc_feature    2315..2737
                     /gene="P35S"
                     /product="Promotor-Region"
                     /SECDrawAs="Region"
                     /SECStyleId=1
                     /SECName="P35S"
                     /SECDescr="Promotor-Region"
     misc_feature    2784..2979
                     /gene="Tocs"
                     /product="Terminator"
                     /SECDrawAs="Region"
                     /SECStyleId=1
                     /SECName="Tocs"
                     /SECDescr="Terminator"
     misc_feature    3064..3087
                     /gene="RB"
                     /product="Right Border"
                     /SECDrawAs="Region"
                     /SECStyleId=1
     misc_feature    3622..4319
                     /gene="ColE1"
                     /product="ColE1 ORI"
                     /SECDrawAs="Region"
                     /SECStyleId=1
     misc_feature    4356..4615
                     /gene="bom"
                     /SECDrawAs="Region"
                     /SECStyleId=1
     misc_feature    4868..8573
                     /gene="pVS1"
                     /product="ori for agrobacteria"
                     /SECDrawAs="Region"
                     /SECStyleId=1
     CDS             8757..9767
                     /gene="Sm/Sp"
                     /product="Strep/Spec resistence"
                     /SECDrawAs="Gene"
                     /SECStyleId=1
ORIGIN      
        1 ggcaggatat attcaattgt aaatggcttc atgtccggga aatctacatg gatcagcaat
       61 gagtatgatg gtcaatatgg agaaaaagaa agagtaatta ccaatttttt ttcaattcaa
      121 aaatgtagat gtccgcagcg ttattataaa atgaaagtac attttgataa aacgacaaat
      181 tacgatccgt cgtatttata ggcgaaagca ataaacaaat tattctaatt cggaaatctt
      241 tatttcgacg tgtctacatt cacgtccaaa tgggggctta gatgagaaac ttcacgatcg
      301 gctctagaac atggtggagc acgacactct cgtctactcc aagaatatca aagatacagt
      361 ctcagaagac cagagggcta ttgagacttt tcaacaaagg gtaatatcgg gaaacctcct
      421 cggattccat tgcccagcta tctgtcactt catcgaaagg acagtagaaa aggaagatgg
      481 cttctacaaa tgccatcatt gcgataaagg aaaggctatc gttcaagatg cctctaccga
      541 cagtggtccc aaagatggac ccccacccac gaggaacatc gtggaaaaag aagacgttcc
      601 aaccacgtct tcaaagcaag tggattgatg tgatatctcc actgacgtaa gggatgacgc
      661 acaatcccac tatccttcgc aagacccttc ctctatataa ggaagttcat ttcatttgga
      721 gaggacctcg agtcctccat aactatgaag aaaccggagc tgaccgccac ttccgtcgaa
      781 aagttcctca tcgagaagtt tgatagcgtg tctgacctta tgcagttgtc ggagggcgaa
      841 gagtcccgcg cgttcagctt tgatgttggc gggcgcgggt acgtcctccg cgtgaactct
      901 tgcgctgacg gcttctataa agatcggtac gtttacaggc atttcgcctc ggcggccctg
      961 cctattcccg aagtcctcga catcggcgag ttttccgaga gccttacgta ttgtatttcc
     1021 cgccgggctc aaggggtgac cctccaggat ctgccggaaa ctgagttgcc tgcggttctc
     1081 cagcccgtcg ccgaggctat ggacgccatc gccgcggcgg atctttctca aacgtcgggc
     1141 ttcgggccgt tcggccctca ggggattggc cagtacacca cttggaggga ctttatctgc
     1201 gctatcgccg atccgcacgt gtatcattgg caaaccgtta tggacgatac ggtctccgcg
     1261 agcgtggccc aggctctgga cgaactcatg ttgtgggccg aggattgccc cgaggtaagt
     1321 ttctgcttct acctttgata tatatataat aattatcatt aattagtagt aatataatat
     1381 ttcaaatatt tttttcaaaa taaaagaatg tagtatatag caattgcttt tctgtagttt
     1441 ataagtgtgt atattttaat ttataacttt tctaatatat gaccaaaatt tgttgatgtg
     1501 caggtccgcc acctggttca tgcggacttc ggctctaata acgtgctcac cgataatggg
     1561 cggattactg ccgtcatcga ctggtccgaa gctatgtttg gcgattcgca gtacgaagtg
     1621 gcgaacattt tcttctggag gccttggctt gcctgcatgg aacagcaaac gcgctacttt
     1681 gagcggaggc acccggagct ggcggggtct ccccgcctca gggcctatat gttgcggatc
     1741 ggcctcgacc agctttacca gtccctggtt gatgggaatt tcgacgatgc tgcctgggcg
     1801 caaggccgct gtgacgctat tgtcaggagc ggcgcgggga ccgtgggccg cactcagatc
     1861 gcccggcggt cggctgccgt ttggacggat gggtgcgtcg aagtgctcgc ggacagcggc
     1921 aacaggcggc cttccacccg cccgcgggcc aaggagtgaa cgcgttcgag tattatggca
     1981 ttgggaaaac tgtttttctt gtaccatttg ttgtgcttgt aatttactgt gttttttatt
     2041 cggttttcgc tatcgaactg tgaaatggaa atggatggag aagagttaat gaatgatatg
     2101 gtccttttgt tcattctcaa attaatatta tttgtttttt ctcttatttg ttgtgtgttg
     2161 aatttgaaat tataagagat atgcaaacat tttgttttga gtaaaaatgt gtcaaatcgt
     2221 ggcctctaat gaccgaagtt aatatgagga gtaaaacact gaagcctgca ggcatgcaag
     2281 ctgatccact agaggccatg gcggccgcac tagatcaaca tggtggagca cgacactctc
     2341 gtctactcca agaatatcaa agatacagtc tcagaagacc agagggctat tgagactttt
     2401 caacaaaggg taatatcggg aaacctcctc ggattccatt gcccagctat ctgtcacttc
     2461 atcgaaagga cagtagaaaa ggaagatggc ttctacaaat gccatcattg cgataaagga
     2521 aaggctatcg ttcaagatgc ctctaccgac agtggtccca aagatggacc cccacccacg
     2581 aggaacatcg tggaaaaaga agacgttcca accacgtctt caaagcaagt ggattgatgt
     2641 gatatctcca ctgacgtaag ggatgacgca caatcccact atccttcgca agacccttcc
     2701 tctatataag gaagttcatt tcatttggag aggacactag ttggatcccc cgggctgcag
     2761 gaattcaagc ttacgcgtgt cgaccctgct ttaatgagat atgcgagacg cctatgatcg
     2821 catgatattt gctttcaatt ctgttgtgca cgttgtaaaa aacctgagca tgtgtagctc
     2881 agatccttac cgccggtttc ggttcattct aatgaatata tcacccgtta ctatcgtatt
     2941 tttatgaata atattctccg ttcaatttac tgattgtcct cgacaggcct taagggccag
     3001 atcttgggcc cggtacccga tcagattgtc gtttcccgcc ttcggtttaa actatcagtg
     3061 tttgacagga tatattggcg ggtaaaccta agagaaaaga gcgtttatta gaataatcgg
     3121 atatttaaaa gggcgtgaaa aggtttatcc gttcgtccat ttgtatgtgc atgccaacca
     3181 cagggttccc ctcgggagtg cttggcattc cgtgcgataa tgacttctgt tcaaccaccc
     3241 aaacgtcgga aagcctgacg acggagcagc attccaaaaa gatcccttgg ctcgtctggg
     3301 tcggctagaa ggtcgagtgg gctgctgtgg cttgatccct caacgcggtc gcggacgtag
     3361 cgcagcgccg aaaaatcctc gatcgcaaat ccgacgctgt cgaaaagcgt gatctgcttg
     3421 tcgctctttc ggccgacgtc ctggccagtc atcacgcgcc aaagttccgt cacaggatga
     3481 tctggcgcga gttgctggat ctcgccttca atccgggtct gtggcgggaa ctccacgaaa
     3541 atatccgaac gcagcaagat atcgcggtgc atctcggtct tgcctgggca gtcgccgccg
     3601 acgccgttga tgtggacgcc gaaaaggatc taggtgaaga tcctttttga taatctcatg
     3661 accaaaatcc cttaacgtga gttttcgttc cactgagcgt cagaccccgt agaaaagatc
     3721 aaaggatctt cttgagatcc tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa
     3781 ccaccgctac cagcggtggt ttgtttgccg gatcaagagc taccaactct ttttccgaag
     3841 gtaactggct tcagcagagc gcagatacca aatactgttc ttctagtgta gccgtagtta
     3901 ggccaccact tcaagaactc tgtagcaccg cctacatacc tcgctctgct aatcctgtta
     3961 ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg ggttggactc aagacgatag
     4021 ttaccggata aggcgcagcg gtcgggctga acggggggtt cgtgcacaca gcccagcttg
     4081 gagcgaacga cctacaccga actgagatac ctacagcgtg agctatgaga aagcgccacg
     4141 cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg aacaggagag
     4201 cgcacgaggg agcttccagg gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc
     4261 cacctctgac ttgagcgtcg atttttgtga tgctcgtcag gggggcggag cctatggaaa
     4321 aacgccagca acgcggcctt tttacggttc ctggcctttt gctggccttt tgctcacatg
     4381 ttctttcctg cgttatcccc tgattctgtg gataaccgat taccgccttt gagtgagctg
     4441 ataccgctcg ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaag
     4501 agcgcctgat gcggtatttt ctccttacgc atctgtgcgg tatttcacac cgcatatggt
     4561 gcactctcag tacaatctgc tctgatgccg catagttaag ccagtataca ctccgctatc
     4621 gctacgtgac tgggtcatgg ctgcgccccg acacccgcca acacccgctg acgcgccctg
     4681 acgggcttgt ctgctcccgg catccgctta cagacaagct gtgaccgtct ccgggagctg
     4741 catgtgtcag aggttttcac cgtcatcacc gaaacgcgcg aggcaggggt acgtcgaggt
     4801 cgatccaacc cctccgctgc tatagtgcag tcggcttctg acgttcagtg cagccgtctt
     4861 ctgaaaacga catgtcgcac aagtcctaag ttacgcgaca ggctgccgcc ctgccctttt
     4921 cctggcgttt tcttgtcgcg tgttttagtc gcataaagta gaatacttgc gactagaacc
     4981 ggagacatta cgccatgaac aagagcgccg ccgctggcct gctgggctat gcccgcgtca
     5041 gcaccgacga ccaggacttg accaaccaac gggccgaact gcacgcggcc ggctgcacca
     5101 agctgttttc cgagaagatc accggcacca ggcgcgaccg cccggagctg gccaggatgc
     5161 ttgaccacct acgccctggc gacgttgtga cagtgaccag gctagaccgc ctggcccgca
     5221 gcacccgcga cctactggac attgccgagc gcatccagga ggccggcgcg ggcctgcgta
     5281 gcctggcaga gccgtgggcc gacaccacca cgccggccgg ccgcatggtg ttgaccgtgt
     5341 tcgccggcat tgccgagttc gagcgttccc taatcatcga ccgcacccgg agcgggcgcg
     5401 aggccgccaa ggcgcgaggc gtgaagtttg gcccccgccc taccctcacc ccggcacaga
     5461 tcgcgcacgc ccgcgagctg atcgaccagg aaggccgcac cgtgaaagag gcggctgcac
     5521 tgcttggcgt gcatcgctcg accctgtacc gcgcacttga gcgcagcgag gaagtgacgc
     5581 ccaccgaggc caggcggcgc ggtgccttcc gtgaggacgc attgaccgag gccgacgccc
     5641 tggcggccgc cgagaatgaa cgccaagagg aacaagcatg aaaccgcacc aggacggcca
     5701 ggacgaaccg tttttcatta ccgaagagat cgaggcggag atgatcgcgg ccgggtacgt
     5761 gttcgagccg cccgcgcacg tctcaaccgt gcggctgcat gaaatcctgg ccggtttgtc
     5821 tgatgccaag ctcgcggcct ggccggcgag cttggccgct gaagaaaccg agcgccgccg
     5881 tctaaaaagg tgatgtgtat ttgagtaaaa cagcttgcgt catgcggtcg ctgcgtatat
     5941 gatgcgatga gtaaataaac aaatacgcaa ggggaacgca tgaaggttat cgctgtactt
     6001 aaccagaaag gcgggtcagg caagacgacc atcgcaaccc atctagcccg cgccctgcaa
     6061 ctcgccgggg ccgatgttct gttagtcgat tccgatcccc agggcagtgc ccgcgattgg
     6121 gcggccgtgc gggaagatca accgctaacc gttgtcggca tcgaccgccc gacgattgac
     6181 cgcgacgtga aggccatcgg ccggcgcgac ttcgtagtga tcgacggagc gccccaggcg
     6241 gcggacttgg ctgtgtccgc gatcaaggca gccgacttcg tgctgattcc ggtgcagcca
     6301 agcccttacg acatatgggc caccgccgac ctggtggagc tggttaagca gcgcattgag
     6361 gtcacggatg gaaggctaca agcggccttt gtcgtgtcgc gggcgatcaa aggcacgcgc
     6421 atcggcggtg aggttgccga ggcgctggcc gggtacgagc tgcccattct tgagtcccgt
     6481 atcacgcagc gcgtgagcta cccaggcact gccgccgccg gcacaaccgt tcttgaatca
     6541 gaacccgagg gcgacgctgc ccgcgaggtc caggcgctgg ccgctgaaat taaatcaaaa
     6601 ctcatttgag ttaatgaggt aaagagaaaa tgagcaaaag cacaaacacg ctaagtgccg
     6661 gccgtccgag cgcacgcagc agcaaggctg caacgttggc cagcctggca gacacgccag
     6721 ccatgaagcg ggtcaacttt cagttgccgg cggaggatca caccaagctg aagatgtacg
     6781 cggtacgcca aggcaagacc attaccgagc tgctatctga atacatcgcg cagctaccag
     6841 agtaaatgag caaatgaata aatgagtaga tgaattttag cggctaaagg aggcggcatg
     6901 gaaaatcaag aacaaccagg caccgacgcc gtggaatgcc ccatgtgtgg aggaacgggc
     6961 ggttggccag gcgtaagcgg ctgggttgtc tgccggccct gcaatggcac tggaaccccc
     7021 aagcccgagg aatcggcgtg agcggtcgca aaccatccgg cccggtacaa atcggcgcgg
     7081 cgctgggtga tgacctggtg gagaagttga aggcggcgca ggccgcccag cggcaacgca
     7141 tcgaggcaga agcacgcccc ggtgaatcgt ggcaagcggc cgctgatcga atccgcaaag
     7201 aatcccggca accgccggca gccggtgcgc cgtcgattag gaagccgccc aagggcgacg
     7261 agcaaccaga ttttttcgtt ccgatgctct atgacgtggg cacccgcgat agtcgcagca
     7321 tcatggacgt ggccgttttc cgtctgtcga agcgtgaccg acgagctggc gaggtgatcc
     7381 gctacgagct tccagacggg cacgtagagg tttccgcagg gccggccggc atggcgagtg
     7441 tgtgggatta cgacctggta ctgatggcgg tttcccatct aaccgaatcc atgaaccgat
     7501 accgggaagg gaagggagac aagcccggcc gcgtgttccg tccacacgtt gcggacgtac
     7561 tcaagttctg ccggcgagcc gatggcggaa agcagaaaga cgacctggta gaaacctgca
     7621 ttcggttaaa caccacgcac gttgccatgc agcgtacgaa gaaggccaag aacggccgcc
     7681 tggtgacggt atccgagggt gaagccttga ttagccgcta caagatcgta aagagcgaaa
     7741 ccgggcggcc ggagtacatc gagatcgagc tagctgattg gatgtaccgc gagatcacag
     7801 aaggcaagaa cccggacgtg ctgacggttc accccgatta ctttttgatc gatcccggca
     7861 tcggccgttt tctctaccgc ctggcacgcc gcgccgcagg caaggcagaa gccagatggt
     7921 tgttcaagac gatctacgaa cgcagtggca gcgccggaga gttcaagaag ttctgtttca
     7981 ccgtgcgcaa gctgatcggg tcaaatgacc tgccggagta cgatttgaag gaggaggcgg
     8041 ggcaggctgg cccgatccta gtcatgcgct accgcaacct gatcgagggc gaagcatccg
     8101 ccggttccta atgtacggag cagatgctag ggcaaattgc cctagcaggg gaaaaaggtc
     8161 gaaaaggtct ctttcctgtg gatagcacgt acattgggaa cccaaagccg tacattggga
     8221 accggaaccc gtacattggg aacccaaagc cgtacattgg gaaccggtca cacatgtaag
     8281 tgactgatat aaaagagaaa aaaggcgatt tttccgccta aaactcttta aaacttatta
     8341 aaactcttaa aacccgcctg gcctgtgcat aactgtctgg ccagcgcaca gccgaagagc
     8401 tgcaaaaagc gcctaccctt cggtcgctgc gctccctacg ccccgccgct tcgcgtcggc
     8461 ctatcgcggc cgctggccgc tcaaaaatgg ctggcctacg gccaggcaat ctaccagggc
     8521 gcggacaagc cgcgccgtcg ccactcgacc gccggcgccc acatcaaggc accggtgggt
     8581 atgcctgacg atgcgtggag accgaaacct tgcgctcgtt cgccagccag gacagaaatg
     8641 cctcgacttc gctgctgccc aaggttgccg ggtgacgcac accgtggaaa cggatgaagg
     8701 cacgaaccca gtggacataa gcctgttcgg ttcgtaagct gtaatgcaag tagcgtatgc
     8761 gctcacgcaa ctggtccaga accttgaccg aacgcagcgg tggtaacggc gcagtggcgg
     8821 ttttcatggc ttgttatgac tgtttttttg gggtacagtc tatgcctcgg gcatccaagc
     8881 agcaagcgcg ttacgccgtg ggtcgatgtt tgatgttatg gagcagcaac gatgttacgc
     8941 agcagggcag tcgccctaaa acaaagttaa acatcatgag ggaagcggtg atcgccgaag
     9001 tatcgactca actatcagag gtagttggcg tcatcgagcg ccatctcgaa ccgacgttgc
     9061 tggccgtaca tttgtacggc tccgcagtgg atggcggcct gaagccacac agtgatattg
     9121 atttgctggt tacggtgacc gtaaggcttg atgaaacaac gcggcgagct ttgatcaacg
     9181 accttttgga aacttcggct tcccctggag agagcgagat tctccgcgct gtagaagtca
     9241 ccattgttgt gcacgacgac atcattccgt ggcgttatcc agctaagcgc gaactgcaat
     9301 ttggagaatg gcagcgcaat gacattcttg caggtatctt cgagccagcc acgatcgaca
     9361 ttgatctggc tatcttgctg acaaaagcaa gagaacatag cgttgccttg gtaggtccag
     9421 cggcggagga actctttgat ccggttcctg aacaggatct atttgaggcg ctaaatgaaa
     9481 ccttaacgct atggaactcg ccgcccgact gggctggcga tgagcgaaat gtagtgctta
     9541 cgttgtcccg catttggtac agcgcagtaa ccggcaaaat cgcgccgaag gatgtcgctg
     9601 ccgactgggc aatggagcgc ctgccggccc agtatcagcc cgtcatactt gaagctagac
     9661 aggcttatct tggacaagaa gaagatcgct tggcctcgcg cgcagatcag ttggaagaat
     9721 ttgtccacta cgtgaaaggc gagatcacca aggtagtcgg caaataatgt ctaacaattc
     9781 gttcaagccg acgccgcttc gcggcgcggc ttaactcaag cgttagatgc actaagcaca
     9841 taattgctca cagccaaact atcaggtcaa gtctgctttt attattttta agcgtgcata
     9901 ataagcccta cacaaattgg gagatatatc atgaaaggct ggctttttct tgttatcgca
     9961 atagttggcg aagtaatcgc aacatagctt gcttggtcgt tccgcgtgaa cgtcggctcg
    10021 attgtacctg cgttcaaata ctttgcgatc gtgttgcgcg cctgcccggt gcgtcggctg
    10081 atctcacgga tcgactgctt ctctcgcaac gccatccgac ggatgatgtt taaaagtccc
    10141 atgtggatca ctccgttgcc ccgtcgctca ccgtgttggg gggaaggtgc acatggctca
    10201 gttctcaatg gaaattatct gcctaaccgg ctcagttctg cgtagaaacc aacatgcaag
    10261 ctccaccggg tgcaaagcgg cagcggc
//
