LOCUS       B424p7o-LH-35s-o        9813 bp    DNA     circular SYN 22-JAN-2016
DEFINITION  Ligation of p35-OCS-BM* into B414p7o-LH*
ACCESSION   B424p7o-LH-35s-o
KEYWORDS    .
SOURCE      Unknown.
  ORGANISM  Unknown
            Unclassified.
REFERENCE   1  (bases 1 to 9813)
  AUTHORS   Self
  JOURNAL   Unpublished.
COMMENT     SECID/File created by Clone Manager, Scientific & Educational Software
COMMENT     SECNOTES|Vector molecule:  B414p7o-LH*
               Fragment ends: SfiI
               Fragment size: 9121
            Insert molecule:  p35-OCS-BM*
               Fragment ends: SfiI
               Fragment size: 692
            Sequencing primers	
            E9F	GTGTCAAATCGTGGCCTCTAA
FEATURES             Location/Qualifiers
     misc_feature    1..24
                     /gene="LB"
                     /product="Left Border"
                     /SECDrawAs="Region"
                     /SECStyleId=1
     misc_feature    309..724
                     /gene="P35S"
                     /product="35S-Promotor"
                     /SECDrawAs="Region"
                     /SECStyleId=1
     CDS             745..978
                     /gene="bar"
                     /SECDrawAs="Gene"
                     /SECStyleId=1
     misc_feature    979..1167
                     /gene="int"
                     /product="STLS1"
                     /SECDrawAs="Gene"
                     /SECStyleId=1
                     /SECName="int"
                     /SECDescr="STLS1"
     CDS             1168..1483
                     /gene="bar"
                     /SECDrawAs="Gene"
                     /SECStyleId=1
     misc_feature    1496..1786
                     /gene="TE9"
                     /product="Terminator Rbcs-E9"
                     /SECDrawAs="Region"
                     /SECStyleId=1
     misc_feature    1841..2263
                     /gene="P35S"
                     /product="Promotor-Region"
                     /SECDrawAs="Region"
                     /SECStyleId=1
                     /SECName="P35S"
                     /SECDescr="Promotor-Region"
     misc_feature    2310..2505
                     /gene="Tocs"
                     /product="Terminator"
                     /SECDrawAs="Region"
                     /SECStyleId=1
                     /SECName="Tocs"
                     /SECDescr="Terminator"
     misc_feature    2590..2613
                     /gene="RB"
                     /product="Right Border"
                     /SECDrawAs="Region"
                     /SECStyleId=1
     misc_feature    3148..3845
                     /gene="ColE1"
                     /product="ColE1 ORI"
                     /SECDrawAs="Region"
                     /SECStyleId=1
     misc_feature    3882..4141
                     /gene="bom"
                     /SECDrawAs="Region"
                     /SECStyleId=1
     misc_feature    4394..8099
                     /gene="pVS1"
                     /product="ori for agrobacteria"
                     /SECDrawAs="Region"
                     /SECStyleId=1
     CDS             8283..9293
                     /gene="Sm/Sp"
                     /product="Strep/Spec resistence"
                     /SECDrawAs="Gene"
                     /SECStyleId=1
ORIGIN      
        1 ggcaggatat attcaattgt aaatggcttc atgtccggga aatctacatg gatcagcaat
       61 gagtatgatg gtcaatatgg agaaaaagaa agagtaatta ccaatttttt ttcaattcaa
      121 aaatgtagat gtccgcagcg ttattataaa atgaaagtac attttgataa aacgacaaat
      181 tacgatccgt cgtatttata ggcgaaagca ataaacaaat tattctaatt cggaaatctt
      241 tatttcgacg tgtctacatt cacgtccaaa tgggggctta gatgagaaac ttcacgatcg
      301 gctctagaac atggtggagc acgacactct cgtctactcc aagaatatca aagatacagt
      361 ctcagaagac cagagggcta ttgagacttt tcaacaaagg gtaatatcgg gaaacctcct
      421 cggattccat tgcccagcta tctgtcactt catcgaaagg acagtagaaa aggaagatgg
      481 cttctacaaa tgccatcatt gcgataaagg aaaggctatc gttcaagatg cctctaccga
      541 cagtggtccc aaagatggac ccccacccac gaggaacatc gtggaaaaag aagacgttcc
      601 aaccacgtct tcaaagcaag tggattgatg tgatatctcc actgacgtaa gggatgacgc
      661 acaatcccac tatccttcgc aagacccttc ctctatataa ggaagttcat ttcatttgga
      721 gaggacctcg agtcctccat aactatgggc cccgagcgca ggcccgccga catccgccgt
      781 gccaccgagg ccgacatgcc agccgtctgc accatcgtga accactacat cgagacctcc
      841 acggtcaact tccgcacaga gccgcaggag cctcaggagt ggaccgacga cctcgtgagg
      901 ctgcgcgagc gctacccctg gctcgtcgct gaggtggatg gcgaggtcgc cggcatcgcc
      961 tacgcgggcc cctggaaggt aagtttctgc ttctaccttt gatatatata taataattat
     1021 cattaattag tagtaatata atatttcaaa tatttttttc aaaataaaag aatgtagtat
     1081 atagcaattg cttttctgta gtttataagt gtgtatattt taatttataa cttttctaat
     1141 atatgaccaa aatttgttga tgtgcaggca cgcaacgcct acgactggac ggcggagagc
     1201 accgtctacg tgtccccacg ccaccagcgc accggcctgg gctccacgct ctacacccac
     1261 ctgctcaaga gcctggaggc ccagggcttc aagtccgtgg tcgctgtgat cggcctcccc
     1321 aacgacccga gcgtccgtat gcacgaggcc ctcggctacg cgccccgcgg gatgctgagg
     1381 gccgcgggct tcaagcacgg caactggcac gacgtgggct tctggcagct cgacttctcc
     1441 ctgcctgtcc caccccgccc ggtgctcccc gtcaccgaaa tctgaacgcg ttcgagtatt
     1501 atggcattgg gaaaactgtt tttcttgtac catttgttgt gcttgtaatt tactgtgttt
     1561 tttattcggt tttcgctatc gaactgtgaa atggaaatgg atggagaaga gttaatgaat
     1621 gatatggtcc ttttgttcat tctcaaatta atattatttg ttttttctct tatttgttgt
     1681 gtgttgaatt tgaaattata agagatatgc aaacattttg ttttgagtaa aaatgtgtca
     1741 aatcgtggcc tctaatgacc gaagttaata tgaggagtaa aacactgaag cctgcaggca
     1801 tgcaagctga tccactagag gccatggcgg ccgcactaga tcaacatggt ggagcacgac
     1861 actctcgtct actccaagaa tatcaaagat acagtctcag aagaccagag ggctattgag
     1921 acttttcaac aaagggtaat atcgggaaac ctcctcggat tccattgccc agctatctgt
     1981 cacttcatcg aaaggacagt agaaaaggaa gatggcttct acaaatgcca tcattgcgat
     2041 aaaggaaagg ctatcgttca agatgcctct accgacagtg gtcccaaaga tggaccccca
     2101 cccacgagga acatcgtgga aaaagaagac gttccaacca cgtcttcaaa gcaagtggat
     2161 tgatgtgata tctccactga cgtaagggat gacgcacaat cccactatcc ttcgcaagac
     2221 ccttcctcta tataaggaag ttcatttcat ttggagagga cactagttgg atcccccggg
     2281 ctgcaggaat tcaagcttac gcgtgtcgac cctgctttaa tgagatatgc gagacgccta
     2341 tgatcgcatg atatttgctt tcaattctgt tgtgcacgtt gtaaaaaacc tgagcatgtg
     2401 tagctcagat ccttaccgcc ggtttcggtt cattctaatg aatatatcac ccgttactat
     2461 cgtattttta tgaataatat tctccgttca atttactgat tgtcctcgac aggccttaag
     2521 ggccagatct tgggcccggt acccgatcag attgtcgttt cccgccttcg gtttaaacta
     2581 tcagtgtttg acaggatata ttggcgggta aacctaagag aaaagagcgt ttattagaat
     2641 aatcggatat ttaaaagggc gtgaaaaggt ttatccgttc gtccatttgt atgtgcatgc
     2701 caaccacagg gttcccctcg ggagtgcttg gcattccgtg cgataatgac ttctgttcaa
     2761 ccacccaaac gtcggaaagc ctgacgacgg agcagcattc caaaaagatc ccttggctcg
     2821 tctgggtcgg ctagaaggtc gagtgggctg ctgtggcttg atccctcaac gcggtcgcgg
     2881 acgtagcgca gcgccgaaaa atcctcgatc gcaaatccga cgctgtcgaa aagcgtgatc
     2941 tgcttgtcgc tctttcggcc gacgtcctgg ccagtcatca cgcgccaaag ttccgtcaca
     3001 ggatgatctg gcgcgagttg ctggatctcg ccttcaatcc gggtctgtgg cgggaactcc
     3061 acgaaaatat ccgaacgcag caagatatcg cggtgcatct cggtcttgcc tgggcagtcg
     3121 ccgccgacgc cgttgatgtg gacgccgaaa aggatctagg tgaagatcct ttttgataat
     3181 ctcatgacca aaatccctta acgtgagttt tcgttccact gagcgtcaga ccccgtagaa
     3241 aagatcaaag gatcttcttg agatcctttt tttctgcgcg taatctgctg cttgcaaaca
     3301 aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc aagagctacc aactcttttt
     3361 ccgaaggtaa ctggcttcag cagagcgcag ataccaaata ctgttcttct agtgtagccg
     3421 tagttaggcc accacttcaa gaactctgta gcaccgccta catacctcgc tctgctaatc
     3481 ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga
     3541 cgatagttac cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc
     3601 agcttggagc gaacgaccta caccgaactg agatacctac agcgtgagct atgagaaagc
     3661 gccacgcttc ccgaagggag aaaggcggac aggtatccgg taagcggcag ggtcggaaca
     3721 ggagagcgca cgagggagct tccaggggga aacgcctggt atctttatag tcctgtcggg
     3781 tttcgccacc tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta
     3841 tggaaaaacg ccagcaacgc ggccttttta cggttcctgg ccttttgctg gccttttgct
     3901 cacatgttct ttcctgcgtt atcccctgat tctgtggata accgattacc gcctttgagt
     3961 gagctgatac cgctcgccgc agccgaacga ccgagcgcag cgagtcagtg agcgaggaag
     4021 cggaagagcg cctgatgcgg tattttctcc ttacgcatct gtgcggtatt tcacaccgca
     4081 tatggtgcac tctcagtaca atctgctctg atgccgcata gttaagccag tatacactcc
     4141 gctatcgcta cgtgactggg tcatggctgc gccccgacac ccgccaacac ccgctgacgc
     4201 gccctgacgg gcttgtctgc tcccggcatc cgcttacaga caagctgtga ccgtctccgg
     4261 gagctgcatg tgtcagaggt tttcaccgtc atcaccgaaa cgcgcgaggc aggggtacgt
     4321 cgaggtcgat ccaacccctc cgctgctata gtgcagtcgg cttctgacgt tcagtgcagc
     4381 cgtcttctga aaacgacatg tcgcacaagt cctaagttac gcgacaggct gccgccctgc
     4441 ccttttcctg gcgttttctt gtcgcgtgtt ttagtcgcat aaagtagaat acttgcgact
     4501 agaaccggag acattacgcc atgaacaaga gcgccgccgc tggcctgctg ggctatgccc
     4561 gcgtcagcac cgacgaccag gacttgacca accaacgggc cgaactgcac gcggccggct
     4621 gcaccaagct gttttccgag aagatcaccg gcaccaggcg cgaccgcccg gagctggcca
     4681 ggatgcttga ccacctacgc cctggcgacg ttgtgacagt gaccaggcta gaccgcctgg
     4741 cccgcagcac ccgcgaccta ctggacattg ccgagcgcat ccaggaggcc ggcgcgggcc
     4801 tgcgtagcct ggcagagccg tgggccgaca ccaccacgcc ggccggccgc atggtgttga
     4861 ccgtgttcgc cggcattgcc gagttcgagc gttccctaat catcgaccgc acccggagcg
     4921 ggcgcgaggc cgccaaggcg cgaggcgtga agtttggccc ccgccctacc ctcaccccgg
     4981 cacagatcgc gcacgcccgc gagctgatcg accaggaagg ccgcaccgtg aaagaggcgg
     5041 ctgcactgct tggcgtgcat cgctcgaccc tgtaccgcgc acttgagcgc agcgaggaag
     5101 tgacgcccac cgaggccagg cggcgcggtg ccttccgtga ggacgcattg accgaggccg
     5161 acgccctggc ggccgccgag aatgaacgcc aagaggaaca agcatgaaac cgcaccagga
     5221 cggccaggac gaaccgtttt tcattaccga agagatcgag gcggagatga tcgcggccgg
     5281 gtacgtgttc gagccgcccg cgcacgtctc aaccgtgcgg ctgcatgaaa tcctggccgg
     5341 tttgtctgat gccaagctcg cggcctggcc ggcgagcttg gccgctgaag aaaccgagcg
     5401 ccgccgtcta aaaaggtgat gtgtatttga gtaaaacagc ttgcgtcatg cggtcgctgc
     5461 gtatatgatg cgatgagtaa ataaacaaat acgcaagggg aacgcatgaa ggttatcgct
     5521 gtacttaacc agaaaggcgg gtcaggcaag acgaccatcg caacccatct agcccgcgcc
     5581 ctgcaactcg ccggggccga tgttctgtta gtcgattccg atccccaggg cagtgcccgc
     5641 gattgggcgg ccgtgcggga agatcaaccg ctaaccgttg tcggcatcga ccgcccgacg
     5701 attgaccgcg acgtgaaggc catcggccgg cgcgacttcg tagtgatcga cggagcgccc
     5761 caggcggcgg acttggctgt gtccgcgatc aaggcagccg acttcgtgct gattccggtg
     5821 cagccaagcc cttacgacat atgggccacc gccgacctgg tggagctggt taagcagcgc
     5881 attgaggtca cggatggaag gctacaagcg gcctttgtcg tgtcgcgggc gatcaaaggc
     5941 acgcgcatcg gcggtgaggt tgccgaggcg ctggccgggt acgagctgcc cattcttgag
     6001 tcccgtatca cgcagcgcgt gagctaccca ggcactgccg ccgccggcac aaccgttctt
     6061 gaatcagaac ccgagggcga cgctgcccgc gaggtccagg cgctggccgc tgaaattaaa
     6121 tcaaaactca tttgagttaa tgaggtaaag agaaaatgag caaaagcaca aacacgctaa
     6181 gtgccggccg tccgagcgca cgcagcagca aggctgcaac gttggccagc ctggcagaca
     6241 cgccagccat gaagcgggtc aactttcagt tgccggcgga ggatcacacc aagctgaaga
     6301 tgtacgcggt acgccaaggc aagaccatta ccgagctgct atctgaatac atcgcgcagc
     6361 taccagagta aatgagcaaa tgaataaatg agtagatgaa ttttagcggc taaaggaggc
     6421 ggcatggaaa atcaagaaca accaggcacc gacgccgtgg aatgccccat gtgtggagga
     6481 acgggcggtt ggccaggcgt aagcggctgg gttgtctgcc ggccctgcaa tggcactgga
     6541 acccccaagc ccgaggaatc ggcgtgagcg gtcgcaaacc atccggcccg gtacaaatcg
     6601 gcgcggcgct gggtgatgac ctggtggaga agttgaaggc ggcgcaggcc gcccagcggc
     6661 aacgcatcga ggcagaagca cgccccggtg aatcgtggca agcggccgct gatcgaatcc
     6721 gcaaagaatc ccggcaaccg ccggcagccg gtgcgccgtc gattaggaag ccgcccaagg
     6781 gcgacgagca accagatttt ttcgttccga tgctctatga cgtgggcacc cgcgatagtc
     6841 gcagcatcat ggacgtggcc gttttccgtc tgtcgaagcg tgaccgacga gctggcgagg
     6901 tgatccgcta cgagcttcca gacgggcacg tagaggtttc cgcagggccg gccggcatgg
     6961 cgagtgtgtg ggattacgac ctggtactga tggcggtttc ccatctaacc gaatccatga
     7021 accgataccg ggaagggaag ggagacaagc ccggccgcgt gttccgtcca cacgttgcgg
     7081 acgtactcaa gttctgccgg cgagccgatg gcggaaagca gaaagacgac ctggtagaaa
     7141 cctgcattcg gttaaacacc acgcacgttg ccatgcagcg tacgaagaag gccaagaacg
     7201 gccgcctggt gacggtatcc gagggtgaag ccttgattag ccgctacaag atcgtaaaga
     7261 gcgaaaccgg gcggccggag tacatcgaga tcgagctagc tgattggatg taccgcgaga
     7321 tcacagaagg caagaacccg gacgtgctga cggttcaccc cgattacttt ttgatcgatc
     7381 ccggcatcgg ccgttttctc taccgcctgg cacgccgcgc cgcaggcaag gcagaagcca
     7441 gatggttgtt caagacgatc tacgaacgca gtggcagcgc cggagagttc aagaagttct
     7501 gtttcaccgt gcgcaagctg atcgggtcaa atgacctgcc ggagtacgat ttgaaggagg
     7561 aggcggggca ggctggcccg atcctagtca tgcgctaccg caacctgatc gagggcgaag
     7621 catccgccgg ttcctaatgt acggagcaga tgctagggca aattgcccta gcaggggaaa
     7681 aaggtcgaaa aggtctcttt cctgtggata gcacgtacat tgggaaccca aagccgtaca
     7741 ttgggaaccg gaacccgtac attgggaacc caaagccgta cattgggaac cggtcacaca
     7801 tgtaagtgac tgatataaaa gagaaaaaag gcgatttttc cgcctaaaac tctttaaaac
     7861 ttattaaaac tcttaaaacc cgcctggcct gtgcataact gtctggccag cgcacagccg
     7921 aagagctgca aaaagcgcct acccttcggt cgctgcgctc cctacgcccc gccgcttcgc
     7981 gtcggcctat cgcggccgct ggccgctcaa aaatggctgg cctacggcca ggcaatctac
     8041 cagggcgcgg acaagccgcg ccgtcgccac tcgaccgccg gcgcccacat caaggcaccg
     8101 gtgggtatgc ctgacgatgc gtggagaccg aaaccttgcg ctcgttcgcc agccaggaca
     8161 gaaatgcctc gacttcgctg ctgcccaagg ttgccgggtg acgcacaccg tggaaacgga
     8221 tgaaggcacg aacccagtgg acataagcct gttcggttcg taagctgtaa tgcaagtagc
     8281 gtatgcgctc acgcaactgg tccagaacct tgaccgaacg cagcggtggt aacggcgcag
     8341 tggcggtttt catggcttgt tatgactgtt tttttggggt acagtctatg cctcgggcat
     8401 ccaagcagca agcgcgttac gccgtgggtc gatgtttgat gttatggagc agcaacgatg
     8461 ttacgcagca gggcagtcgc cctaaaacaa agttaaacat catgagggaa gcggtgatcg
     8521 ccgaagtatc gactcaacta tcagaggtag ttggcgtcat cgagcgccat ctcgaaccga
     8581 cgttgctggc cgtacatttg tacggctccg cagtggatgg cggcctgaag ccacacagtg
     8641 atattgattt gctggttacg gtgaccgtaa ggcttgatga aacaacgcgg cgagctttga
     8701 tcaacgacct tttggaaact tcggcttccc ctggagagag cgagattctc cgcgctgtag
     8761 aagtcaccat tgttgtgcac gacgacatca ttccgtggcg ttatccagct aagcgcgaac
     8821 tgcaatttgg agaatggcag cgcaatgaca ttcttgcagg tatcttcgag ccagccacga
     8881 tcgacattga tctggctatc ttgctgacaa aagcaagaga acatagcgtt gccttggtag
     8941 gtccagcggc ggaggaactc tttgatccgg ttcctgaaca ggatctattt gaggcgctaa
     9001 atgaaacctt aacgctatgg aactcgccgc ccgactgggc tggcgatgag cgaaatgtag
     9061 tgcttacgtt gtcccgcatt tggtacagcg cagtaaccgg caaaatcgcg ccgaaggatg
     9121 tcgctgccga ctgggcaatg gagcgcctgc cggcccagta tcagcccgtc atacttgaag
     9181 ctagacaggc ttatcttgga caagaagaag atcgcttggc ctcgcgcgca gatcagttgg
     9241 aagaatttgt ccactacgtg aaaggcgaga tcaccaaggt agtcggcaaa taatgtctaa
     9301 caattcgttc aagccgacgc cgcttcgcgg cgcggcttaa ctcaagcgtt agatgcacta
     9361 agcacataat tgctcacagc caaactatca ggtcaagtct gcttttatta tttttaagcg
     9421 tgcataataa gccctacaca aattgggaga tatatcatga aaggctggct ttttcttgtt
     9481 atcgcaatag ttggcgaagt aatcgcaaca tagcttgctt ggtcgttccg cgtgaacgtc
     9541 ggctcgattg tacctgcgtt caaatacttt gcgatcgtgt tgcgcgcctg cccggtgcgt
     9601 cggctgatct cacggatcga ctgcttctct cgcaacgcca tccgacggat gatgtttaaa
     9661 agtcccatgt ggatcactcc gttgccccgt cgctcaccgt gttgggggga aggtgcacat
     9721 ggctcagttc tcaatggaaa ttatctgcct aaccggctca gttctgcgta gaaaccaaca
     9781 tgcaagctcc accgggtgca aagcggcagc ggc
//
