LOCUS       B708p7o-LH-LGFP        10352 bp    DNA     circular SYN 22-JAN-2016
DEFINITION  Ligation of B636pLGFP-ocs* into B414p7o-LH*
ACCESSION   B708p7o-LH-LGFP
KEYWORDS    .
SOURCE      Unknown.
  ORGANISM  Unknown
            Unclassified.
REFERENCE   1  (bases 1 to 10352)
  AUTHORS   Self
  JOURNAL   Unpublished.
COMMENT     SECID/File created by Clone Manager, Scientific & Educational Software
COMMENT     SECNOTES|Vector molecule:  B414p7o-LH*
               Fragment ends: SfiI
               Fragment size: 9121
            Insert molecule:  B636pLGFP-ocs*
               Fragment ends: SfiI
               Fragment size: 1231
            Sequencing primers	
            GFP-seq	CCAGTTCCACCAGGATTG
            OF2	CCGGTTTCGGTTCATTCTAA
FEATURES             Location/Qualifiers
     misc_feature    1..24
                     /gene="LB"
                     /product="Left Border"
                     /SECDrawAs="Region"
                     /SECStyleId=1
     misc_feature    309..724
                     /gene="P35S"
                     /product="35S-Promotor"
                     /SECDrawAs="Region"
                     /SECStyleId=1
     CDS             745..978
                     /gene="bar"
                     /SECDrawAs="Gene"
                     /SECStyleId=1
     misc_feature    979..1167
                     /gene="int"
                     /product="STLS1"
                     /SECDrawAs="Gene"
                     /SECStyleId=1
                     /SECName="int"
                     /SECDescr="STLS1"
     CDS             1168..1483
                     /gene="bar"
                     /SECDrawAs="Gene"
                     /SECStyleId=1
     misc_feature    1496..1786
                     /gene="TE9"
                     /product="Terminator Rbcs-E9"
                     /SECDrawAs="Region"
                     /SECStyleId=1
     misc_signal     1883..1933
                     /label=link
                     /SECDrawAs="Label"
     CDS             1934..2312
                     /gene="gfpm"
                     /product="GFPm"
                     /SECDrawAs="Gene"
                     /SECStyleId=1
     CDS             2313..2501
                     /gene="int"
                     /product="STLS1"
                     /SECDrawAs="Gene"
                     /SECStyleId=1
     CDS             2502..2839
                     /gene="gfpm"
                     /product="GFPm"
                     /SECDrawAs="Gene"
                     /SECStyleId=1
     misc_feature    2849..3044
                     /gene="Tocs"
                     /product="Terminator"
                     /SECDrawAs="Region"
                     /SECStyleId=1
                     /SECName="Tocs"
                     /SECDescr="Terminator"
     misc_feature    3129..3152
                     /gene="RB"
                     /product="Right Border"
                     /SECDrawAs="Region"
                     /SECStyleId=1
     misc_feature    3687..4384
                     /gene="ColE1"
                     /product="ColE1 ORI"
                     /SECDrawAs="Region"
                     /SECStyleId=1
     misc_feature    4421..4680
                     /gene="bom"
                     /SECDrawAs="Region"
                     /SECStyleId=1
     misc_feature    4933..8638
                     /gene="pVS1"
                     /product="ori for agrobacteria"
                     /SECDrawAs="Region"
                     /SECStyleId=1
     CDS             8822..9832
                     /gene="Sm/Sp"
                     /product="Strep/Spec resistence"
                     /SECDrawAs="Gene"
                     /SECStyleId=1
ORIGIN      
        1 ggcaggatat attcaattgt aaatggcttc atgtccggga aatctacatg gatcagcaat
       61 gagtatgatg gtcaatatgg agaaaaagaa agagtaatta ccaatttttt ttcaattcaa
      121 aaatgtagat gtccgcagcg ttattataaa atgaaagtac attttgataa aacgacaaat
      181 tacgatccgt cgtatttata ggcgaaagca ataaacaaat tattctaatt cggaaatctt
      241 tatttcgacg tgtctacatt cacgtccaaa tgggggctta gatgagaaac ttcacgatcg
      301 gctctagaac atggtggagc acgacactct cgtctactcc aagaatatca aagatacagt
      361 ctcagaagac cagagggcta ttgagacttt tcaacaaagg gtaatatcgg gaaacctcct
      421 cggattccat tgcccagcta tctgtcactt catcgaaagg acagtagaaa aggaagatgg
      481 cttctacaaa tgccatcatt gcgataaagg aaaggctatc gttcaagatg cctctaccga
      541 cagtggtccc aaagatggac ccccacccac gaggaacatc gtggaaaaag aagacgttcc
      601 aaccacgtct tcaaagcaag tggattgatg tgatatctcc actgacgtaa gggatgacgc
      661 acaatcccac tatccttcgc aagacccttc ctctatataa ggaagttcat ttcatttgga
      721 gaggacctcg agtcctccat aactatgggc cccgagcgca ggcccgccga catccgccgt
      781 gccaccgagg ccgacatgcc agccgtctgc accatcgtga accactacat cgagacctcc
      841 acggtcaact tccgcacaga gccgcaggag cctcaggagt ggaccgacga cctcgtgagg
      901 ctgcgcgagc gctacccctg gctcgtcgct gaggtggatg gcgaggtcgc cggcatcgcc
      961 tacgcgggcc cctggaaggt aagtttctgc ttctaccttt gatatatata taataattat
     1021 cattaattag tagtaatata atatttcaaa tatttttttc aaaataaaag aatgtagtat
     1081 atagcaattg cttttctgta gtttataagt gtgtatattt taatttataa cttttctaat
     1141 atatgaccaa aatttgttga tgtgcaggca cgcaacgcct acgactggac ggcggagagc
     1201 accgtctacg tgtccccacg ccaccagcgc accggcctgg gctccacgct ctacacccac
     1261 ctgctcaaga gcctggaggc ccagggcttc aagtccgtgg tcgctgtgat cggcctcccc
     1321 aacgacccga gcgtccgtat gcacgaggcc ctcggctacg cgccccgcgg gatgctgagg
     1381 gccgcgggct tcaagcacgg caactggcac gacgtgggct tctggcagct cgacttctcc
     1441 ctgcctgtcc caccccgccc ggtgctcccc gtcaccgaaa tctgaacgcg ttcgagtatt
     1501 atggcattgg gaaaactgtt tttcttgtac catttgttgt gcttgtaatt tactgtgttt
     1561 tttattcggt tttcgctatc gaactgtgaa atggaaatgg atggagaaga gttaatgaat
     1621 gatatggtcc ttttgttcat tctcaaatta atattatttg ttttttctct tatttgttgt
     1681 gtgttgaatt tgaaattata agagatatgc aaacattttg ttttgagtaa aaatgtgtca
     1741 aatcgtggcc tctaatgacc gaagttaata tgaggagtaa aacactgaag cctgcaggca
     1801 tgcaagctga tccactagag gccatggcgg ccgcactagt ggatcccccg ggctgcagga
     1861 attcaagctt acgcgttgta caggagtacc gccccgtccg gtcctgcccg tcaccgagat
     1921 cagcggagag ttcatgggca agggcgagga actgttcact ggcgtggtcc caatcctggt
     1981 ggaactggat ggtgatgtga acgggcacaa gttctccgtc agcggagagg gtgaaggtga
     2041 tgccacctac ggaaagctca ccctgaagtt catctgcact accggaaagc tccctgttcc
     2101 gtggccaacc ctcgtcacca ctttcaccta cggtgttcag tgcttctccc gttacccaga
     2161 tcacatgaag cagcatgact tcttcaagag cgccatgccc gaaggctacg tgcaagaaag
     2221 gactatcttc ttcaaggatg acgggaacta caagacacgt gccgaagtca agttcgaagg
     2281 tgataccctg gtgaaccgca tcgagctgaa aggtaagttt ctgcttctac ctttgatata
     2341 tatataataa ttatcattaa ttagtagtaa tataatattt caaatatttt tttcaaaata
     2401 aaagaatgta gtatatagca attgcttttc tgtagtttat aagtgtgtat attttaattt
     2461 ataacttttc taatatatga ccaaaatttg ttgatgtgca ggtatcgatt tcaaggaaga
     2521 tggaaacatc ctcggacaca agctggagta caactacaac tcccacaacg tatacatcat
     2581 ggccgacaag cagaagaacg gcatcaaggt gaacttcaag atcaggcaca acatcgaaga
     2641 tggaagcgtg caactggcgg accactacca gcagaacacg cccatcggcg atggccctgt
     2701 cctgctgccg gacaaccatt acctgtccac gcaatctgcc ctctccaagg accccaacga
     2761 gaagagggac cacatggtcc tgctggagtt cgtgacggct gctgggatca cgcatggcat
     2821 ggatgaactc tacaagtgac ccggtcgacc ctgctttaat gagatatgcg agacgcctat
     2881 gatcgcatga tatttgcttt caattctgtt gtgcacgttg taaaaaacct gagcatgtgt
     2941 agctcagatc cttaccgccg gtttcggttc attctaatga atatatcacc cgttactatc
     3001 gtatttttat gaataatatt ctccgttcaa tttactgatt gtcctcgaca ggccttaagg
     3061 gccagatctt gggcccggta cccgatcaga ttgtcgtttc ccgccttcgg tttaaactat
     3121 cagtgtttga caggatatat tggcgggtaa acctaagaga aaagagcgtt tattagaata
     3181 atcggatatt taaaagggcg tgaaaaggtt tatccgttcg tccatttgta tgtgcatgcc
     3241 aaccacaggg ttcccctcgg gagtgcttgg cattccgtgc gataatgact tctgttcaac
     3301 cacccaaacg tcggaaagcc tgacgacgga gcagcattcc aaaaagatcc cttggctcgt
     3361 ctgggtcggc tagaaggtcg agtgggctgc tgtggcttga tccctcaacg cggtcgcgga
     3421 cgtagcgcag cgccgaaaaa tcctcgatcg caaatccgac gctgtcgaaa agcgtgatct
     3481 gcttgtcgct ctttcggccg acgtcctggc cagtcatcac gcgccaaagt tccgtcacag
     3541 gatgatctgg cgcgagttgc tggatctcgc cttcaatccg ggtctgtggc gggaactcca
     3601 cgaaaatatc cgaacgcagc aagatatcgc ggtgcatctc ggtcttgcct gggcagtcgc
     3661 cgccgacgcc gttgatgtgg acgccgaaaa ggatctaggt gaagatcctt tttgataatc
     3721 tcatgaccaa aatcccttaa cgtgagtttt cgttccactg agcgtcagac cccgtagaaa
     3781 agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa
     3841 aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc
     3901 cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgttcttcta gtgtagccgt
     3961 agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc
     4021 tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac
     4081 gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca
     4141 gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta tgagaaagcg
     4201 ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag
     4261 gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt
     4321 ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat
     4381 ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg ccttttgctc
     4441 acatgttctt tcctgcgtta tcccctgatt ctgtggataa ccgattaccg cctttgagtg
     4501 agctgatacc gctcgccgca gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc
     4561 ggaagagcgc ctgatgcggt attttctcct tacgcatctg tgcggtattt cacaccgcat
     4621 atggtgcact ctcagtacaa tctgctctga tgccgcatag ttaagccagt atacactccg
     4681 ctatcgctac gtgactgggt catggctgcg ccccgacacc cgccaacacc cgctgacgcg
     4741 ccctgacggg cttgtctgct cccggcatcc gcttacagac aagctgtgac cgtctccggg
     4801 agctgcatgt gtcagaggtt ttcaccgtca tcaccgaaac gcgcgaggca ggggtacgtc
     4861 gaggtcgatc caacccctcc gctgctatag tgcagtcggc ttctgacgtt cagtgcagcc
     4921 gtcttctgaa aacgacatgt cgcacaagtc ctaagttacg cgacaggctg ccgccctgcc
     4981 cttttcctgg cgttttcttg tcgcgtgttt tagtcgcata aagtagaata cttgcgacta
     5041 gaaccggaga cattacgcca tgaacaagag cgccgccgct ggcctgctgg gctatgcccg
     5101 cgtcagcacc gacgaccagg acttgaccaa ccaacgggcc gaactgcacg cggccggctg
     5161 caccaagctg ttttccgaga agatcaccgg caccaggcgc gaccgcccgg agctggccag
     5221 gatgcttgac cacctacgcc ctggcgacgt tgtgacagtg accaggctag accgcctggc
     5281 ccgcagcacc cgcgacctac tggacattgc cgagcgcatc caggaggccg gcgcgggcct
     5341 gcgtagcctg gcagagccgt gggccgacac caccacgccg gccggccgca tggtgttgac
     5401 cgtgttcgcc ggcattgccg agttcgagcg ttccctaatc atcgaccgca cccggagcgg
     5461 gcgcgaggcc gccaaggcgc gaggcgtgaa gtttggcccc cgccctaccc tcaccccggc
     5521 acagatcgcg cacgcccgcg agctgatcga ccaggaaggc cgcaccgtga aagaggcggc
     5581 tgcactgctt ggcgtgcatc gctcgaccct gtaccgcgca cttgagcgca gcgaggaagt
     5641 gacgcccacc gaggccaggc ggcgcggtgc cttccgtgag gacgcattga ccgaggccga
     5701 cgccctggcg gccgccgaga atgaacgcca agaggaacaa gcatgaaacc gcaccaggac
     5761 ggccaggacg aaccgttttt cattaccgaa gagatcgagg cggagatgat cgcggccggg
     5821 tacgtgttcg agccgcccgc gcacgtctca accgtgcggc tgcatgaaat cctggccggt
     5881 ttgtctgatg ccaagctcgc ggcctggccg gcgagcttgg ccgctgaaga aaccgagcgc
     5941 cgccgtctaa aaaggtgatg tgtatttgag taaaacagct tgcgtcatgc ggtcgctgcg
     6001 tatatgatgc gatgagtaaa taaacaaata cgcaagggga acgcatgaag gttatcgctg
     6061 tacttaacca gaaaggcggg tcaggcaaga cgaccatcgc aacccatcta gcccgcgccc
     6121 tgcaactcgc cggggccgat gttctgttag tcgattccga tccccagggc agtgcccgcg
     6181 attgggcggc cgtgcgggaa gatcaaccgc taaccgttgt cggcatcgac cgcccgacga
     6241 ttgaccgcga cgtgaaggcc atcggccggc gcgacttcgt agtgatcgac ggagcgcccc
     6301 aggcggcgga cttggctgtg tccgcgatca aggcagccga cttcgtgctg attccggtgc
     6361 agccaagccc ttacgacata tgggccaccg ccgacctggt ggagctggtt aagcagcgca
     6421 ttgaggtcac ggatggaagg ctacaagcgg cctttgtcgt gtcgcgggcg atcaaaggca
     6481 cgcgcatcgg cggtgaggtt gccgaggcgc tggccgggta cgagctgccc attcttgagt
     6541 cccgtatcac gcagcgcgtg agctacccag gcactgccgc cgccggcaca accgttcttg
     6601 aatcagaacc cgagggcgac gctgcccgcg aggtccaggc gctggccgct gaaattaaat
     6661 caaaactcat ttgagttaat gaggtaaaga gaaaatgagc aaaagcacaa acacgctaag
     6721 tgccggccgt ccgagcgcac gcagcagcaa ggctgcaacg ttggccagcc tggcagacac
     6781 gccagccatg aagcgggtca actttcagtt gccggcggag gatcacacca agctgaagat
     6841 gtacgcggta cgccaaggca agaccattac cgagctgcta tctgaataca tcgcgcagct
     6901 accagagtaa atgagcaaat gaataaatga gtagatgaat tttagcggct aaaggaggcg
     6961 gcatggaaaa tcaagaacaa ccaggcaccg acgccgtgga atgccccatg tgtggaggaa
     7021 cgggcggttg gccaggcgta agcggctggg ttgtctgccg gccctgcaat ggcactggaa
     7081 cccccaagcc cgaggaatcg gcgtgagcgg tcgcaaacca tccggcccgg tacaaatcgg
     7141 cgcggcgctg ggtgatgacc tggtggagaa gttgaaggcg gcgcaggccg cccagcggca
     7201 acgcatcgag gcagaagcac gccccggtga atcgtggcaa gcggccgctg atcgaatccg
     7261 caaagaatcc cggcaaccgc cggcagccgg tgcgccgtcg attaggaagc cgcccaaggg
     7321 cgacgagcaa ccagattttt tcgttccgat gctctatgac gtgggcaccc gcgatagtcg
     7381 cagcatcatg gacgtggccg ttttccgtct gtcgaagcgt gaccgacgag ctggcgaggt
     7441 gatccgctac gagcttccag acgggcacgt agaggtttcc gcagggccgg ccggcatggc
     7501 gagtgtgtgg gattacgacc tggtactgat ggcggtttcc catctaaccg aatccatgaa
     7561 ccgataccgg gaagggaagg gagacaagcc cggccgcgtg ttccgtccac acgttgcgga
     7621 cgtactcaag ttctgccggc gagccgatgg cggaaagcag aaagacgacc tggtagaaac
     7681 ctgcattcgg ttaaacacca cgcacgttgc catgcagcgt acgaagaagg ccaagaacgg
     7741 ccgcctggtg acggtatccg agggtgaagc cttgattagc cgctacaaga tcgtaaagag
     7801 cgaaaccggg cggccggagt acatcgagat cgagctagct gattggatgt accgcgagat
     7861 cacagaaggc aagaacccgg acgtgctgac ggttcacccc gattactttt tgatcgatcc
     7921 cggcatcggc cgttttctct accgcctggc acgccgcgcc gcaggcaagg cagaagccag
     7981 atggttgttc aagacgatct acgaacgcag tggcagcgcc ggagagttca agaagttctg
     8041 tttcaccgtg cgcaagctga tcgggtcaaa tgacctgccg gagtacgatt tgaaggagga
     8101 ggcggggcag gctggcccga tcctagtcat gcgctaccgc aacctgatcg agggcgaagc
     8161 atccgccggt tcctaatgta cggagcagat gctagggcaa attgccctag caggggaaaa
     8221 aggtcgaaaa ggtctctttc ctgtggatag cacgtacatt gggaacccaa agccgtacat
     8281 tgggaaccgg aacccgtaca ttgggaaccc aaagccgtac attgggaacc ggtcacacat
     8341 gtaagtgact gatataaaag agaaaaaagg cgatttttcc gcctaaaact ctttaaaact
     8401 tattaaaact cttaaaaccc gcctggcctg tgcataactg tctggccagc gcacagccga
     8461 agagctgcaa aaagcgccta cccttcggtc gctgcgctcc ctacgccccg ccgcttcgcg
     8521 tcggcctatc gcggccgctg gccgctcaaa aatggctggc ctacggccag gcaatctacc
     8581 agggcgcgga caagccgcgc cgtcgccact cgaccgccgg cgcccacatc aaggcaccgg
     8641 tgggtatgcc tgacgatgcg tggagaccga aaccttgcgc tcgttcgcca gccaggacag
     8701 aaatgcctcg acttcgctgc tgcccaaggt tgccgggtga cgcacaccgt ggaaacggat
     8761 gaaggcacga acccagtgga cataagcctg ttcggttcgt aagctgtaat gcaagtagcg
     8821 tatgcgctca cgcaactggt ccagaacctt gaccgaacgc agcggtggta acggcgcagt
     8881 ggcggttttc atggcttgtt atgactgttt ttttggggta cagtctatgc ctcgggcatc
     8941 caagcagcaa gcgcgttacg ccgtgggtcg atgtttgatg ttatggagca gcaacgatgt
     9001 tacgcagcag ggcagtcgcc ctaaaacaaa gttaaacatc atgagggaag cggtgatcgc
     9061 cgaagtatcg actcaactat cagaggtagt tggcgtcatc gagcgccatc tcgaaccgac
     9121 gttgctggcc gtacatttgt acggctccgc agtggatggc ggcctgaagc cacacagtga
     9181 tattgatttg ctggttacgg tgaccgtaag gcttgatgaa acaacgcggc gagctttgat
     9241 caacgacctt ttggaaactt cggcttcccc tggagagagc gagattctcc gcgctgtaga
     9301 agtcaccatt gttgtgcacg acgacatcat tccgtggcgt tatccagcta agcgcgaact
     9361 gcaatttgga gaatggcagc gcaatgacat tcttgcaggt atcttcgagc cagccacgat
     9421 cgacattgat ctggctatct tgctgacaaa agcaagagaa catagcgttg ccttggtagg
     9481 tccagcggcg gaggaactct ttgatccggt tcctgaacag gatctatttg aggcgctaaa
     9541 tgaaacctta acgctatgga actcgccgcc cgactgggct ggcgatgagc gaaatgtagt
     9601 gcttacgttg tcccgcattt ggtacagcgc agtaaccggc aaaatcgcgc cgaaggatgt
     9661 cgctgccgac tgggcaatgg agcgcctgcc ggcccagtat cagcccgtca tacttgaagc
     9721 tagacaggct tatcttggac aagaagaaga tcgcttggcc tcgcgcgcag atcagttgga
     9781 agaatttgtc cactacgtga aaggcgagat caccaaggta gtcggcaaat aatgtctaac
     9841 aattcgttca agccgacgcc gcttcgcggc gcggcttaac tcaagcgtta gatgcactaa
     9901 gcacataatt gctcacagcc aaactatcag gtcaagtctg cttttattat ttttaagcgt
     9961 gcataataag ccctacacaa attgggagat atatcatgaa aggctggctt tttcttgtta
    10021 tcgcaatagt tggcgaagta atcgcaacat agcttgcttg gtcgttccgc gtgaacgtcg
    10081 gctcgattgt acctgcgttc aaatactttg cgatcgtgtt gcgcgcctgc ccggtgcgtc
    10141 ggctgatctc acggatcgac tgcttctctc gcaacgccat ccgacggatg atgtttaaaa
    10201 gtcccatgtg gatcactccg ttgccccgtc gctcaccgtg ttggggggaa ggtgcacatg
    10261 gctcagttct caatggaaat tatctgccta accggctcag ttctgcgtag aaaccaacat
    10321 gcaagctcca ccgggtgcaa agcggcagcg gc
//
