LOCUS B418p9oUH-LH 9363 bp DNA circular SYN 22-JAN-2016 DEFINITION Ligation of UbiH-BsmB-Xho* into B415p9o-LH* ACCESSION B418p9oUH-LH KEYWORDS . SOURCE Unknown. ORGANISM Unknown Unclassified. REFERENCE 1 (bases 1 to 9363) AUTHORS Self JOURNAL Unpublished. COMMENT SECID/File created by Clone Manager, Scientific & Educational Software COMMENT SECNOTES|Vector molecule: B415p9o-LH* Fragment ends: XhoI and XbaI Fragment size: 9013 Insert molecule: UbiH-BsmB-Xho* Fragment ends: BsmBI and XhoI Fragment size: 350 Primer for gene synthesis UbiHBsmB ccCGTCTCtctagaCCGCGACAAAACTTAAG UbiHXho CCCGTCctcgagcAGACGAAGCGAAACAG Sequencing primers LHX1 CTTTATTTCGACGTGTCTAC LB CTCAGTTCTGCGTAGAAACCA Maxi FEATURES Location/Qualifiers misc_feature 1..24 /gene="LB" /product="Left Border" /SECDrawAs="Region" /SECStyleId=1 misc_feature 316..651 /gene="UbiZm" /product="Promotor" /SECDrawAs="Region" /SECStyleId=1 misc_signal 433..435 /label=cat /SECDrawAs="Label" misc_signal 436..438 /label=cat /SECDrawAs="Label" misc_signal 469..471 /label=cat /SECDrawAs="Label" misc_signal 498..500 /label=cat /SECDrawAs="Label" misc_signal 509..511 /label=cat /SECDrawAs="Label" misc_signal 530..532 /label=cat /SECDrawAs="Label" misc_signal 533..535 /label=cat /SECDrawAs="Label" misc_signal 538..541 /label=tata /SECDrawAs="Label" CDS 569..651 /gene="mRNA" /SECDrawAs="Gene" /SECStyleId=1 CDS 671..969 /gene="nptIIo" /product="NptII optimiert" /SECDrawAs="Gene" /SECStyleId=1 misc_signal 966..969 /label=splice /SECDrawAs="Label" CDS 970..1156 /gene="int" /SECDrawAs="Gene" /SECStyleId=1 misc_signal 1155..1158 /label=splice /SECDrawAs="Label" CDS 1157..1660 /gene="nptIIo" /product="NptII optimiert" /SECDrawAs="Gene" /SECStyleId=1 misc_feature 1674..1964 /gene="TE9" /product="Terminator Rbcs-E9" /SECDrawAs="Region" /SECStyleId=1 misc_feature 2140..2163 /gene="RB" /product="Right Border" /SECDrawAs="Region" /SECStyleId=1 misc_feature 2698..3395 /gene="ColE1" /product="ColE1 ORI" /SECDrawAs="Region" /SECStyleId=1 misc_feature 3432..3691 /gene="bom" /SECDrawAs="Region" /SECStyleId=1 misc_feature 3944..7649 /gene="pVS1" /product="ori for agrobacteria" /SECDrawAs="Region" /SECStyleId=1 CDS 7833..8843 /gene="Sm/Sp" /product="Strep/Spec resistence" /SECDrawAs="Gene" /SECStyleId=1 ORIGIN 1 ggcaggatat attcaattgt aaatggcttc atgtccggga aatctacatg gatcagcaat 61 gagtatgatg gtcaatatgg agaaaaagaa agagtaatta ccaatttttt ttcaattcaa 121 aaatgtagat gtccgcagcg ttattataaa atgaaagtac attttgataa aacgacaaat 181 tacgatccgt cgtatttata ggcgaaagca ataaacaaat tattctaatt cggaaatctt 241 tatttcgacg tgtctacatt cacgtccaaa tgggggctta gatgagaaac ttcacgatcg 301 gctctagacc gcgacaaaac ttaagaaagt caccaaaaag aaagtcaaag acggcaacgg 361 caacccagaa gctacgttct ctttctagat tctatgccct acctaccgcg tggtgcacaa 421 caacttgacc cccatcattg actctccacc gtacacgtgt cacgatacca tttgttggtc 481 tcctaccact tacgtaacat taatattcca tccgtgaccg cctcttcttc atcatcctat 541 aaattcacct tcttatctct tccgctttca ttcgttcatc atcatcacaa ttaacaacaa 601 tccaaagcaa tcttctctca tattcttatc tcttcctgtt tcgcttcgtc tgctcgagtc 661 ctccataact atgggagcat ggattgagca ggatggcctc catgctggta gccccgctgc 721 ttgggtggag cgcctctttg gctacgactg ggcccaacag accattgggt gcagtgatgc 781 cgctgtcttc cgtttgagcg cccagggcag gccagttctc ttcgtgaaga ctgacctctc 841 cggtgccctc aacgagttgc aggacgaagc tgcccgcctc tcatggcttg ccaccaccgg 901 agtcccttgc gctgccgtgt tggacgttgt cacagaggcc ggaagagact ggctgctctt 961 gggcgaggta agtttctgct tctacctttg atatatatat aataattatc attaattagt 1021 agtaatataa tatttcaaat atttttttca aaataaaaga atgtagtata tagcaattgc 1081 ttttctgtag tttataagtg tgtatatttt aatttataac ttttctaata tatgaccaaa 1141 atttgttgat gtgcaggtgc caggccaaga cctcctgagc tcccacctgg ccccagccga 1201 gaaggtctcc atcatggcag acgccatgcg tcgcctccac accctggacc cagccacctg 1261 ccccttcgac caccaggcca agcacaggat cgaacgtgca cgcactagga tggaggccgg 1321 ccttgtggac caggatgacc ttgacgagga gcaccagggc cttgctcccg ccgagctgtt 1381 cgcccgcctc aaggctagaa tgcctgacgg agaggacttg gtggtcaccc acggggatgc 1441 ctgcctcccc aacatcatgg ttgagaacgg acgcttctct ggcttcatcg actgcggtcg 1501 tctgggcgtg gccgaccgct accaagacat cgccctggcc accagggaca tcgctgagga 1561 acttggaggg gagtgggccg atcgtttctt ggtcctctac ggcattgctg cccccgactc 1621 ccagaggatc gccttctacc gcctgcttga cgagttcttc tgaacgcgtt cgagtattat 1681 ggcattggga aaactgtttt tcttgtacca tttgttgtgc ttgtaattta ctgtgttttt 1741 tattcggttt tcgctatcga actgtgaaat ggaaatggat ggagaagagt taatgaatga 1801 tatggtcctt ttgttcattc tcaaattaat attatttgtt ttttctctta tttgttgtgt 1861 gttgaatttg aaattataag agatatgcaa acattttgtt ttgagtaaaa atgtgtcaaa 1921 tcgtggcctc taatgaccga agttaatatg aggagtaaaa cactgaagcc tgcaggcatg 1981 caagctgatc cactagaggc catggcggcc gcactagtgg atcccccggg atgcatgaat 2041 tcaagcttat cgatgtcgac aggccttaag ggccagatct tgggcccggt acccgatcag 2101 attgtcgttt cccgccttcg gtttaaacta tcagtgtttg acaggatata ttggcgggta 2161 aacctaagag aaaagagcgt ttattagaat aatcggatat ttaaaagggc gtgaaaaggt 2221 ttatccgttc gtccatttgt atgtgcatgc caaccacagg gttcccctcg ggagtgcttg 2281 gcattccgtg cgataatgac ttctgttcaa ccacccaaac gtcggaaagc ctgacgacgg 2341 agcagcattc caaaaagatc ccttggctcg tctgggtcgg ctagaaggtc gagtgggctg 2401 ctgtggcttg atccctcaac gcggtcgcgg acgtagcgca gcgccgaaaa atcctcgatc 2461 gcaaatccga cgctgtcgaa aagcgtgatc tgcttgtcgc tctttcggcc gacgtcctgg 2521 ccagtcatca cgcgccaaag ttccgtcaca ggatgatctg gcgcgagttg ctggatctcg 2581 ccttcaatcc gggtctgtgg cgggaactcc acgaaaatat ccgaacgcag caagatatcg 2641 cggtgcatct cggtcttgcc tgggcagtcg ccgccgacgc cgttgatgtg gacgccgaaa 2701 aggatctagg tgaagatcct ttttgataat ctcatgacca aaatccctta acgtgagttt 2761 tcgttccact gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg agatcctttt 2821 tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt 2881 ttgccggatc aagagctacc aactcttttt ccgaaggtaa ctggcttcag cagagcgcag 2941 ataccaaata ctgttcttct agtgtagccg tagttaggcc accacttcaa gaactctgta 3001 gcaccgccta catacctcgc tctgctaatc ctgttaccag tggctgctgc cagtggcgat 3061 aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac cggataaggc gcagcggtcg 3121 ggctgaacgg ggggttcgtg cacacagccc agcttggagc gaacgaccta caccgaactg 3181 agatacctac agcgtgagct atgagaaagc gccacgcttc ccgaagggag aaaggcggac 3241 aggtatccgg taagcggcag ggtcggaaca ggagagcgca cgagggagct tccaggggga 3301 aacgcctggt atctttatag tcctgtcggg tttcgccacc tctgacttga gcgtcgattt 3361 ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc ggccttttta 3421 cggttcctgg ccttttgctg gccttttgct cacatgttct ttcctgcgtt atcccctgat 3481 tctgtggata accgattacc gcctttgagt gagctgatac cgctcgccgc agccgaacga 3541 ccgagcgcag cgagtcagtg agcgaggaag cggaagagcg cctgatgcgg tattttctcc 3601 ttacgcatct gtgcggtatt tcacaccgca tatggtgcac tctcagtaca atctgctctg 3661 atgccgcata gttaagccag tatacactcc gctatcgcta cgtgactggg tcatggctgc 3721 gccccgacac ccgccaacac ccgctgacgc gccctgacgg gcttgtctgc tcccggcatc 3781 cgcttacaga caagctgtga ccgtctccgg gagctgcatg tgtcagaggt tttcaccgtc 3841 atcaccgaaa cgcgcgaggc aggggtacgt cgaggtcgat ccaacccctc cgctgctata 3901 gtgcagtcgg cttctgacgt tcagtgcagc cgtcttctga aaacgacatg tcgcacaagt 3961 cctaagttac gcgacaggct gccgccctgc ccttttcctg gcgttttctt gtcgcgtgtt 4021 ttagtcgcat aaagtagaat acttgcgact agaaccggag acattacgcc atgaacaaga 4081 gcgccgccgc tggcctgctg ggctatgccc gcgtcagcac cgacgaccag gacttgacca 4141 accaacgggc cgaactgcac gcggccggct gcaccaagct gttttccgag aagatcaccg 4201 gcaccaggcg cgaccgcccg gagctggcca ggatgcttga ccacctacgc cctggcgacg 4261 ttgtgacagt gaccaggcta gaccgcctgg cccgcagcac ccgcgaccta ctggacattg 4321 ccgagcgcat ccaggaggcc ggcgcgggcc tgcgtagcct ggcagagccg tgggccgaca 4381 ccaccacgcc ggccggccgc atggtgttga ccgtgttcgc cggcattgcc gagttcgagc 4441 gttccctaat catcgaccgc acccggagcg ggcgcgaggc cgccaaggcg cgaggcgtga 4501 agtttggccc ccgccctacc ctcaccccgg cacagatcgc gcacgcccgc gagctgatcg 4561 accaggaagg ccgcaccgtg aaagaggcgg ctgcactgct tggcgtgcat cgctcgaccc 4621 tgtaccgcgc acttgagcgc agcgaggaag tgacgcccac cgaggccagg cggcgcggtg 4681 ccttccgtga ggacgcattg accgaggccg acgccctggc ggccgccgag aatgaacgcc 4741 aagaggaaca agcatgaaac cgcaccagga cggccaggac gaaccgtttt tcattaccga 4801 agagatcgag gcggagatga tcgcggccgg gtacgtgttc gagccgcccg cgcacgtctc 4861 aaccgtgcgg ctgcatgaaa tcctggccgg tttgtctgat gccaagctcg cggcctggcc 4921 ggcgagcttg gccgctgaag aaaccgagcg ccgccgtcta aaaaggtgat gtgtatttga 4981 gtaaaacagc ttgcgtcatg cggtcgctgc gtatatgatg cgatgagtaa ataaacaaat 5041 acgcaagggg aacgcatgaa ggttatcgct gtacttaacc agaaaggcgg gtcaggcaag 5101 acgaccatcg caacccatct agcccgcgcc ctgcaactcg ccggggccga tgttctgtta 5161 gtcgattccg atccccaggg cagtgcccgc gattgggcgg ccgtgcggga agatcaaccg 5221 ctaaccgttg tcggcatcga ccgcccgacg attgaccgcg acgtgaaggc catcggccgg 5281 cgcgacttcg tagtgatcga cggagcgccc caggcggcgg acttggctgt gtccgcgatc 5341 aaggcagccg acttcgtgct gattccggtg cagccaagcc cttacgacat atgggccacc 5401 gccgacctgg tggagctggt taagcagcgc attgaggtca cggatggaag gctacaagcg 5461 gcctttgtcg tgtcgcgggc gatcaaaggc acgcgcatcg gcggtgaggt tgccgaggcg 5521 ctggccgggt acgagctgcc cattcttgag tcccgtatca cgcagcgcgt gagctaccca 5581 ggcactgccg ccgccggcac aaccgttctt gaatcagaac ccgagggcga cgctgcccgc 5641 gaggtccagg cgctggccgc tgaaattaaa tcaaaactca tttgagttaa tgaggtaaag 5701 agaaaatgag caaaagcaca aacacgctaa gtgccggccg tccgagcgca cgcagcagca 5761 aggctgcaac gttggccagc ctggcagaca cgccagccat gaagcgggtc aactttcagt 5821 tgccggcgga ggatcacacc aagctgaaga tgtacgcggt acgccaaggc aagaccatta 5881 ccgagctgct atctgaatac atcgcgcagc taccagagta aatgagcaaa tgaataaatg 5941 agtagatgaa ttttagcggc taaaggaggc ggcatggaaa atcaagaaca accaggcacc 6001 gacgccgtgg aatgccccat gtgtggagga acgggcggtt ggccaggcgt aagcggctgg 6061 gttgtctgcc ggccctgcaa tggcactgga acccccaagc ccgaggaatc ggcgtgagcg 6121 gtcgcaaacc atccggcccg gtacaaatcg gcgcggcgct gggtgatgac ctggtggaga 6181 agttgaaggc ggcgcaggcc gcccagcggc aacgcatcga ggcagaagca cgccccggtg 6241 aatcgtggca agcggccgct gatcgaatcc gcaaagaatc ccggcaaccg ccggcagccg 6301 gtgcgccgtc gattaggaag ccgcccaagg gcgacgagca accagatttt ttcgttccga 6361 tgctctatga cgtgggcacc cgcgatagtc gcagcatcat ggacgtggcc gttttccgtc 6421 tgtcgaagcg tgaccgacga gctggcgagg tgatccgcta cgagcttcca gacgggcacg 6481 tagaggtttc cgcagggccg gccggcatgg cgagtgtgtg ggattacgac ctggtactga 6541 tggcggtttc ccatctaacc gaatccatga accgataccg ggaagggaag ggagacaagc 6601 ccggccgcgt gttccgtcca cacgttgcgg acgtactcaa gttctgccgg cgagccgatg 6661 gcggaaagca gaaagacgac ctggtagaaa cctgcattcg gttaaacacc acgcacgttg 6721 ccatgcagcg tacgaagaag gccaagaacg gccgcctggt gacggtatcc gagggtgaag 6781 ccttgattag ccgctacaag atcgtaaaga gcgaaaccgg gcggccggag tacatcgaga 6841 tcgagctagc tgattggatg taccgcgaga tcacagaagg caagaacccg gacgtgctga 6901 cggttcaccc cgattacttt ttgatcgatc ccggcatcgg ccgttttctc taccgcctgg 6961 cacgccgcgc cgcaggcaag gcagaagcca gatggttgtt caagacgatc tacgaacgca 7021 gtggcagcgc cggagagttc aagaagttct gtttcaccgt gcgcaagctg atcgggtcaa 7081 atgacctgcc ggagtacgat ttgaaggagg aggcggggca ggctggcccg atcctagtca 7141 tgcgctaccg caacctgatc gagggcgaag catccgccgg ttcctaatgt acggagcaga 7201 tgctagggca aattgcccta gcaggggaaa aaggtcgaaa aggtctcttt cctgtggata 7261 gcacgtacat tgggaaccca aagccgtaca ttgggaaccg gaacccgtac attgggaacc 7321 caaagccgta cattgggaac cggtcacaca tgtaagtgac tgatataaaa gagaaaaaag 7381 gcgatttttc cgcctaaaac tctttaaaac ttattaaaac tcttaaaacc cgcctggcct 7441 gtgcataact gtctggccag cgcacagccg aagagctgca aaaagcgcct acccttcggt 7501 cgctgcgctc cctacgcccc gccgcttcgc gtcggcctat cgcggccgct ggccgctcaa 7561 aaatggctgg cctacggcca ggcaatctac cagggcgcgg acaagccgcg ccgtcgccac 7621 tcgaccgccg gcgcccacat caaggcaccg gtgggtatgc ctgacgatgc gtggagaccg 7681 aaaccttgcg ctcgttcgcc agccaggaca gaaatgcctc gacttcgctg ctgcccaagg 7741 ttgccgggtg acgcacaccg tggaaacgga tgaaggcacg aacccagtgg acataagcct 7801 gttcggttcg taagctgtaa tgcaagtagc gtatgcgctc acgcaactgg tccagaacct 7861 tgaccgaacg cagcggtggt aacggcgcag tggcggtttt catggcttgt tatgactgtt 7921 tttttggggt acagtctatg cctcgggcat ccaagcagca agcgcgttac gccgtgggtc 7981 gatgtttgat gttatggagc agcaacgatg ttacgcagca gggcagtcgc cctaaaacaa 8041 agttaaacat catgagggaa gcggtgatcg ccgaagtatc gactcaacta tcagaggtag 8101 ttggcgtcat cgagcgccat ctcgaaccga cgttgctggc cgtacatttg tacggctccg 8161 cagtggatgg cggcctgaag ccacacagtg atattgattt gctggttacg gtgaccgtaa 8221 ggcttgatga aacaacgcgg cgagctttga tcaacgacct tttggaaact tcggcttccc 8281 ctggagagag cgagattctc cgcgctgtag aagtcaccat tgttgtgcac gacgacatca 8341 ttccgtggcg ttatccagct aagcgcgaac tgcaatttgg agaatggcag cgcaatgaca 8401 ttcttgcagg tatcttcgag ccagccacga tcgacattga tctggctatc ttgctgacaa 8461 aagcaagaga acatagcgtt gccttggtag gtccagcggc ggaggaactc tttgatccgg 8521 ttcctgaaca ggatctattt gaggcgctaa atgaaacctt aacgctatgg aactcgccgc 8581 ccgactgggc tggcgatgag cgaaatgtag tgcttacgtt gtcccgcatt tggtacagcg 8641 cagtaaccgg caaaatcgcg ccgaaggatg tcgctgccga ctgggcaatg gagcgcctgc 8701 cggcccagta tcagcccgtc atacttgaag ctagacaggc ttatcttgga caagaagaag 8761 atcgcttggc ctcgcgcgca gatcagttgg aagaatttgt ccactacgtg aaaggcgaga 8821 tcaccaaggt agtcggcaaa taatgtctaa caattcgttc aagccgacgc cgcttcgcgg 8881 cgcggcttaa ctcaagcgtt agatgcacta agcacataat tgctcacagc caaactatca 8941 ggtcaagtct gcttttatta tttttaagcg tgcataataa gccctacaca aattgggaga 9001 tatatcatga aaggctggct ttttcttgtt atcgcaatag ttggcgaagt aatcgcaaca 9061 tagcttgctt ggtcgttccg cgtgaacgtc ggctcgattg tacctgcgtt caaatacttt 9121 gcgatcgtgt tgcgcgcctg cccggtgcgt cggctgatct cacggatcga ctgcttctct 9181 cgcaacgcca tccgacggat gatgtttaaa agtcccatgt ggatcactcc gttgccccgt 9241 cgctcaccgt gttgggggga aggtgcacat ggctcagttc tcaatggaaa ttatctgcct 9301 aaccggctca gttctgcgta gaaaccaaca tgcaagctcc accgggtgca aagcggcagc 9361 ggc //