LOCUS       B433p6oU10-LH          10718 bp    DNA     circular SYN 22-JAN-2016
DEFINITION  Ligation of U10-Bsa-Xho* into B413p6o-LH*
ACCESSION   B433p6oU10-LH
KEYWORDS    .
SOURCE      Unknown.
  ORGANISM  Unknown
            Unclassified.
REFERENCE   1  (bases 1 to 10718)
  AUTHORS   Self
  JOURNAL   Unpublished.
COMMENT     SECID/File created by Clone Manager, Scientific & Educational Software
COMMENT     SECNOTES|Vector molecule:  B413p6o-LH*
               Fragment ends: XhoI and XbaI
               Fragment size: 9235
            Insert molecule:  U10-Bsa-Xho*
               Fragment ends: BsaI and XhoI
               Fragment size: 1483
            Primer for gene synthesis
            BsaU10	ccGGTCTCtctagaTTTCCTTACATTCTGAGCC
            XhoU10	CCCGTCctcgaGTACCTGTTAATCAGAAAAACTC
            Sequencing primers	
            LHX1	CTTTATTTCGACGTGTCTAC
            hptoR	GGTCAGACACGCTATCAAACT
            
            
FEATURES             Location/Qualifiers
     misc_feature    1..24
                     /gene="LB"
                     /product="Left Border"
                     /SECDrawAs="Region"
                     /SECStyleId=1
     misc_feature    309..1784
                     /gene="UBQ10"
                     /product="PUbq10At"
                     /SECDrawAs="Region"
                     /SECStyleId=1
     misc_feature    1413..1478
                     /product="Ubi cds 5"
                     /SECDrawAs="Region"
                     /SECStyleId=1
     misc_feature    1479..1782
                     /gene="int"
                     /SECDrawAs="Region"
                     /SECStyleId=1
     CDS             1804..2373
                     /gene="hptII"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGR
                     GYVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQD
                     LPETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVY
                     HWQTVMDDTVSASVAQALDELMLWAEDCPE"
                     /SECDrawAs="Gene"
                     /SECStyleId=1
                     /SECName="hptII"
     misc_feature    2374..2562
                     /gene="int"
                     /product="StLS1"
                     /SECDrawAs="Gene"
                     /SECStyleId=1
                     /SECName="int"
                     /SECDescr="StLS1"
     CDS             2563..3018
                     /gene="hptII"
                     /codon_start=3
                     /SECDrawAs="Gene"
                     /SECStyleId=1
     misc_feature    3029..3319
                     /gene="TE9"
                     /product="Terminator Rbcs-E9"
                     /SECDrawAs="Region"
                     /SECStyleId=1
     misc_feature    3495..3518
                     /gene="RB"
                     /product="Right Border"
                     /SECDrawAs="Region"
                     /SECStyleId=1
     misc_feature    4053..4750
                     /gene="ColE1"
                     /product="ColE1 ORI"
                     /SECDrawAs="Region"
                     /SECStyleId=1
     misc_feature    4787..5046
                     /gene="bom"
                     /SECDrawAs="Region"
                     /SECStyleId=1
     misc_feature    5299..9004
                     /gene="pVS1"
                     /product="ori for agrobacteria"
                     /SECDrawAs="Region"
                     /SECStyleId=1
     CDS             9188..10198
                     /gene="Sm/Sp"
                     /product="Strep/Spec resistence"
                     /SECDrawAs="Gene"
                     /SECStyleId=1
ORIGIN      
        1 ggcaggatat attcaattgt aaatggcttc atgtccggga aatctacatg gatcagcaat
       61 gagtatgatg gtcaatatgg agaaaaagaa agagtaatta ccaatttttt ttcaattcaa
      121 aaatgtagat gtccgcagcg ttattataaa atgaaagtac attttgataa aacgacaaat
      181 tacgatccgt cgtatttata ggcgaaagca ataaacaaat tattctaatt cggaaatctt
      241 tatttcgacg tgtctacatt cacgtccaaa tgggggctta gatgagaaac ttcacgatcg
      301 gctctagatt tccttacatt ctgagcctct ttccttctaa tccactcatc tgcatcttct
      361 tgtgtcctta ctaatacctc attggttcca aattccctcc ctttaagcac cagctcgttt
      421 ctgttcttcc acagcctccc aagtatccaa gggactaaag cctccacatt cttcagatca
      481 ggatattctt gtttaagatg ttgaactcta tggaggtttg tatgaactga tgatctagga
      541 ccggataagt tcccttcttc atagcgaact tattcaaaga atgttttgtg tatcattctt
      601 gttacattgt tattaatgaa aaaatattat tggtcattgg actgaacacg agtgttaaat
      661 atggaccagg ccccaaataa gatccattga tatatgaatt aaataacaag aataaatcga
      721 gtcaccaaac cacttgcctt ttttaacgag acttgttcac caacttgata caaaagtcat
      781 tatcctatgc aaatcaataa tcatacaaaa atatccaata acactaaaaa attaaaagaa
      841 atggataatt tcacaatatg ttatacgata aagaagttac ttttccaaga aattcactga
      901 ttttataagc ccacttgcat tagataaatg gcaaaaaaaa acaaaaagga aaagaaataa
      961 agcacgaaga attctagaaa atacgaaata cgcttcaatg cagtgggacc cacggttcaa
     1021 ttattgccaa ttttcagctc caccgtatat ttaaaaaata aaacgataat gctaaaaaaa
     1081 tataaatcgt aacgatcgtt aaatctcaac ggctggatct tatgacgacc gttagaaatt
     1141 gtggttgtcg acgagtcagt aataaacggc gtcaaagtgg ttgcagccgg cacacacgag
     1201 tcgtgtttat caactcaaag cacaaatact tttcctcaac ctaaaaataa ggcaattagc
     1261 caaaaacaac tttgcgtgta aacaacgctc aatacacgtg tcattttatt attagctatt
     1321 gcttcaccgc cttagctttc tcgtgaccta gtcgtcctcg tcttttcttc ttcttcttct
     1381 ataaaacaat acccaaagag ctcttcttct tcacaattca gatttcaatt tctcaaaatc
     1441 ttaaaaactt tctctcaatt ctctctaccg tgatcaaggt aaatttctgt gttccttatt
     1501 ctctcaaaat cttcgatttt gttttcgttc gatcccaatt tcgtatatgt tctttggttt
     1561 agattctgtt aatcttagat cgaagacgat tttctgggtt tgatcgttag atatcatctt
     1621 aattctcgat tagggtttca tagatatcat ccgatttgtt caaataattt gagttttgtc
     1681 gaataattac tcttcgattt gtgatttcta tctagatctg gtgttagttt ctagtttgtg
     1741 cgatcgaatt tgtcgattaa tctgagtttt tctgattaac aggtactcga gtcctccata
     1801 actatgaaga aaccggagct gaccgccact tccgtcgaaa agttcctcat cgagaagttt
     1861 gatagcgtgt ctgaccttat gcagttgtcg gagggcgaag agtcccgcgc gttcagcttt
     1921 gatgttggcg ggcgcgggta cgtcctccgc gtgaactctt gcgctgacgg cttctataaa
     1981 gatcggtacg tttacaggca tttcgcctcg gcggccctgc ctattcccga agtcctcgac
     2041 atcggcgagt tttccgagag ccttacgtat tgtatttccc gccgggctca aggggtgacc
     2101 ctccaggatc tgccggaaac tgagttgcct gcggttctcc agcccgtcgc cgaggctatg
     2161 gacgccatcg ccgcggcgga tctttctcaa acgtcgggct tcgggccgtt cggccctcag
     2221 gggattggcc agtacaccac ttggagggac tttatctgcg ctatcgccga tccgcacgtg
     2281 tatcattggc aaaccgttat ggacgatacg gtctccgcga gcgtggccca ggctctggac
     2341 gaactcatgt tgtgggccga ggattgcccc gaggtaagtt tctgcttcta cctttgatat
     2401 atatataata attatcatta attagtagta atataatatt tcaaatattt ttttcaaaat
     2461 aaaagaatgt agtatatagc aattgctttt ctgtagttta taagtgtgta tattttaatt
     2521 tataactttt ctaatatatg accaaaattt gttgatgtgc aggtccgcca cctggttcat
     2581 gcggacttcg gctctaataa cgtgctcacc gataatgggc ggattactgc cgtcatcgac
     2641 tggtccgaag ctatgtttgg cgattcgcag tacgaagtgg cgaacatttt cttctggagg
     2701 ccttggcttg cctgcatgga acagcaaacg cgctactttg agcggaggca cccggagctg
     2761 gcggggtctc cccgcctcag ggcctatatg ttgcggatcg gcctcgacca gctttaccag
     2821 tccctggttg atgggaattt cgacgatgct gcctgggcgc aaggccgctg tgacgctatt
     2881 gtcaggagcg gcgcggggac cgtgggccgc actcagatcg cccggcggtc ggctgccgtt
     2941 tggacggatg ggtgcgtcga agtgctcgcg gacagcggca acaggcggcc ttccacccgc
     3001 ccgcgggcca aggagtgaac gcgttcgagt attatggcat tgggaaaact gtttttcttg
     3061 taccatttgt tgtgcttgta atttactgtg ttttttattc ggttttcgct atcgaactgt
     3121 gaaatggaaa tggatggaga agagttaatg aatgatatgg tccttttgtt cattctcaaa
     3181 ttaatattat ttgttttttc tcttatttgt tgtgtgttga atttgaaatt ataagagata
     3241 tgcaaacatt ttgttttgag taaaaatgtg tcaaatcgtg gcctctaatg accgaagtta
     3301 atatgaggag taaaacactg aagcctgcag gcatgcaagc tgatccacta gaggccatgg
     3361 cggccgcact agtggatccc ccgggatgca tgaattcaag cttatcgatg tcgacaggcc
     3421 ttaagggcca gatcttgggc ccggtacccg atcagattgt cgtttcccgc cttcggttta
     3481 aactatcagt gtttgacagg atatattggc gggtaaacct aagagaaaag agcgtttatt
     3541 agaataatcg gatatttaaa agggcgtgaa aaggtttatc cgttcgtcca tttgtatgtg
     3601 catgccaacc acagggttcc cctcgggagt gcttggcatt ccgtgcgata atgacttctg
     3661 ttcaaccacc caaacgtcgg aaagcctgac gacggagcag cattccaaaa agatcccttg
     3721 gctcgtctgg gtcggctaga aggtcgagtg ggctgctgtg gcttgatccc tcaacgcggt
     3781 cgcggacgta gcgcagcgcc gaaaaatcct cgatcgcaaa tccgacgctg tcgaaaagcg
     3841 tgatctgctt gtcgctcttt cggccgacgt cctggccagt catcacgcgc caaagttccg
     3901 tcacaggatg atctggcgcg agttgctgga tctcgccttc aatccgggtc tgtggcggga
     3961 actccacgaa aatatccgaa cgcagcaaga tatcgcggtg catctcggtc ttgcctgggc
     4021 agtcgccgcc gacgccgttg atgtggacgc cgaaaaggat ctaggtgaag atcctttttg
     4081 ataatctcat gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg tcagaccccg
     4141 tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc
     4201 aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc
     4261 tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtt cttctagtgt
     4321 agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc
     4381 taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact
     4441 caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac
     4501 agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag
     4561 aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg
     4621 gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg
     4681 tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga
     4741 gcctatggaa aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt
     4801 ttgctcacat gttctttcct gcgttatccc ctgattctgt ggataaccga ttaccgcctt
     4861 tgagtgagct gataccgctc gccgcagccg aacgaccgag cgcagcgagt cagtgagcga
     4921 ggaagcggaa gagcgcctga tgcggtattt tctccttacg catctgtgcg gtatttcaca
     4981 ccgcatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac
     5041 actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct
     5101 gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc
     5161 tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagggg
     5221 tacgtcgagg tcgatccaac ccctccgctg ctatagtgca gtcggcttct gacgttcagt
     5281 gcagccgtct tctgaaaacg acatgtcgca caagtcctaa gttacgcgac aggctgccgc
     5341 cctgcccttt tcctggcgtt ttcttgtcgc gtgttttagt cgcataaagt agaatacttg
     5401 cgactagaac cggagacatt acgccatgaa caagagcgcc gccgctggcc tgctgggcta
     5461 tgcccgcgtc agcaccgacg accaggactt gaccaaccaa cgggccgaac tgcacgcggc
     5521 cggctgcacc aagctgtttt ccgagaagat caccggcacc aggcgcgacc gcccggagct
     5581 ggccaggatg cttgaccacc tacgccctgg cgacgttgtg acagtgacca ggctagaccg
     5641 cctggcccgc agcacccgcg acctactgga cattgccgag cgcatccagg aggccggcgc
     5701 gggcctgcgt agcctggcag agccgtgggc cgacaccacc acgccggccg gccgcatggt
     5761 gttgaccgtg ttcgccggca ttgccgagtt cgagcgttcc ctaatcatcg accgcacccg
     5821 gagcgggcgc gaggccgcca aggcgcgagg cgtgaagttt ggcccccgcc ctaccctcac
     5881 cccggcacag atcgcgcacg cccgcgagct gatcgaccag gaaggccgca ccgtgaaaga
     5941 ggcggctgca ctgcttggcg tgcatcgctc gaccctgtac cgcgcacttg agcgcagcga
     6001 ggaagtgacg cccaccgagg ccaggcggcg cggtgccttc cgtgaggacg cattgaccga
     6061 ggccgacgcc ctggcggccg ccgagaatga acgccaagag gaacaagcat gaaaccgcac
     6121 caggacggcc aggacgaacc gtttttcatt accgaagaga tcgaggcgga gatgatcgcg
     6181 gccgggtacg tgttcgagcc gcccgcgcac gtctcaaccg tgcggctgca tgaaatcctg
     6241 gccggtttgt ctgatgccaa gctcgcggcc tggccggcga gcttggccgc tgaagaaacc
     6301 gagcgccgcc gtctaaaaag gtgatgtgta tttgagtaaa acagcttgcg tcatgcggtc
     6361 gctgcgtata tgatgcgatg agtaaataaa caaatacgca aggggaacgc atgaaggtta
     6421 tcgctgtact taaccagaaa ggcgggtcag gcaagacgac catcgcaacc catctagccc
     6481 gcgccctgca actcgccggg gccgatgttc tgttagtcga ttccgatccc cagggcagtg
     6541 cccgcgattg ggcggccgtg cgggaagatc aaccgctaac cgttgtcggc atcgaccgcc
     6601 cgacgattga ccgcgacgtg aaggccatcg gccggcgcga cttcgtagtg atcgacggag
     6661 cgccccaggc ggcggacttg gctgtgtccg cgatcaaggc agccgacttc gtgctgattc
     6721 cggtgcagcc aagcccttac gacatatggg ccaccgccga cctggtggag ctggttaagc
     6781 agcgcattga ggtcacggat ggaaggctac aagcggcctt tgtcgtgtcg cgggcgatca
     6841 aaggcacgcg catcggcggt gaggttgccg aggcgctggc cgggtacgag ctgcccattc
     6901 ttgagtcccg tatcacgcag cgcgtgagct acccaggcac tgccgccgcc ggcacaaccg
     6961 ttcttgaatc agaacccgag ggcgacgctg cccgcgaggt ccaggcgctg gccgctgaaa
     7021 ttaaatcaaa actcatttga gttaatgagg taaagagaaa atgagcaaaa gcacaaacac
     7081 gctaagtgcc ggccgtccga gcgcacgcag cagcaaggct gcaacgttgg ccagcctggc
     7141 agacacgcca gccatgaagc gggtcaactt tcagttgccg gcggaggatc acaccaagct
     7201 gaagatgtac gcggtacgcc aaggcaagac cattaccgag ctgctatctg aatacatcgc
     7261 gcagctacca gagtaaatga gcaaatgaat aaatgagtag atgaatttta gcggctaaag
     7321 gaggcggcat ggaaaatcaa gaacaaccag gcaccgacgc cgtggaatgc cccatgtgtg
     7381 gaggaacggg cggttggcca ggcgtaagcg gctgggttgt ctgccggccc tgcaatggca
     7441 ctggaacccc caagcccgag gaatcggcgt gagcggtcgc aaaccatccg gcccggtaca
     7501 aatcggcgcg gcgctgggtg atgacctggt ggagaagttg aaggcggcgc aggccgccca
     7561 gcggcaacgc atcgaggcag aagcacgccc cggtgaatcg tggcaagcgg ccgctgatcg
     7621 aatccgcaaa gaatcccggc aaccgccggc agccggtgcg ccgtcgatta ggaagccgcc
     7681 caagggcgac gagcaaccag attttttcgt tccgatgctc tatgacgtgg gcacccgcga
     7741 tagtcgcagc atcatggacg tggccgtttt ccgtctgtcg aagcgtgacc gacgagctgg
     7801 cgaggtgatc cgctacgagc ttccagacgg gcacgtagag gtttccgcag ggccggccgg
     7861 catggcgagt gtgtgggatt acgacctggt actgatggcg gtttcccatc taaccgaatc
     7921 catgaaccga taccgggaag ggaagggaga caagcccggc cgcgtgttcc gtccacacgt
     7981 tgcggacgta ctcaagttct gccggcgagc cgatggcgga aagcagaaag acgacctggt
     8041 agaaacctgc attcggttaa acaccacgca cgttgccatg cagcgtacga agaaggccaa
     8101 gaacggccgc ctggtgacgg tatccgaggg tgaagccttg attagccgct acaagatcgt
     8161 aaagagcgaa accgggcggc cggagtacat cgagatcgag ctagctgatt ggatgtaccg
     8221 cgagatcaca gaaggcaaga acccggacgt gctgacggtt caccccgatt actttttgat
     8281 cgatcccggc atcggccgtt ttctctaccg cctggcacgc cgcgccgcag gcaaggcaga
     8341 agccagatgg ttgttcaaga cgatctacga acgcagtggc agcgccggag agttcaagaa
     8401 gttctgtttc accgtgcgca agctgatcgg gtcaaatgac ctgccggagt acgatttgaa
     8461 ggaggaggcg gggcaggctg gcccgatcct agtcatgcgc taccgcaacc tgatcgaggg
     8521 cgaagcatcc gccggttcct aatgtacgga gcagatgcta gggcaaattg ccctagcagg
     8581 ggaaaaaggt cgaaaaggtc tctttcctgt ggatagcacg tacattggga acccaaagcc
     8641 gtacattggg aaccggaacc cgtacattgg gaacccaaag ccgtacattg ggaaccggtc
     8701 acacatgtaa gtgactgata taaaagagaa aaaaggcgat ttttccgcct aaaactcttt
     8761 aaaacttatt aaaactctta aaacccgcct ggcctgtgca taactgtctg gccagcgcac
     8821 agccgaagag ctgcaaaaag cgcctaccct tcggtcgctg cgctccctac gccccgccgc
     8881 ttcgcgtcgg cctatcgcgg ccgctggccg ctcaaaaatg gctggcctac ggccaggcaa
     8941 tctaccaggg cgcggacaag ccgcgccgtc gccactcgac cgccggcgcc cacatcaagg
     9001 caccggtggg tatgcctgac gatgcgtgga gaccgaaacc ttgcgctcgt tcgccagcca
     9061 ggacagaaat gcctcgactt cgctgctgcc caaggttgcc gggtgacgca caccgtggaa
     9121 acggatgaag gcacgaaccc agtggacata agcctgttcg gttcgtaagc tgtaatgcaa
     9181 gtagcgtatg cgctcacgca actggtccag aaccttgacc gaacgcagcg gtggtaacgg
     9241 cgcagtggcg gttttcatgg cttgttatga ctgttttttt ggggtacagt ctatgcctcg
     9301 ggcatccaag cagcaagcgc gttacgccgt gggtcgatgt ttgatgttat ggagcagcaa
     9361 cgatgttacg cagcagggca gtcgccctaa aacaaagtta aacatcatga gggaagcggt
     9421 gatcgccgaa gtatcgactc aactatcaga ggtagttggc gtcatcgagc gccatctcga
     9481 accgacgttg ctggccgtac atttgtacgg ctccgcagtg gatggcggcc tgaagccaca
     9541 cagtgatatt gatttgctgg ttacggtgac cgtaaggctt gatgaaacaa cgcggcgagc
     9601 tttgatcaac gaccttttgg aaacttcggc ttcccctgga gagagcgaga ttctccgcgc
     9661 tgtagaagtc accattgttg tgcacgacga catcattccg tggcgttatc cagctaagcg
     9721 cgaactgcaa tttggagaat ggcagcgcaa tgacattctt gcaggtatct tcgagccagc
     9781 cacgatcgac attgatctgg ctatcttgct gacaaaagca agagaacata gcgttgcctt
     9841 ggtaggtcca gcggcggagg aactctttga tccggttcct gaacaggatc tatttgaggc
     9901 gctaaatgaa accttaacgc tatggaactc gccgcccgac tgggctggcg atgagcgaaa
     9961 tgtagtgctt acgttgtccc gcatttggta cagcgcagta accggcaaaa tcgcgccgaa
    10021 ggatgtcgct gccgactggg caatggagcg cctgccggcc cagtatcagc ccgtcatact
    10081 tgaagctaga caggcttatc ttggacaaga agaagatcgc ttggcctcgc gcgcagatca
    10141 gttggaagaa tttgtccact acgtgaaagg cgagatcacc aaggtagtcg gcaaataatg
    10201 tctaacaatt cgttcaagcc gacgccgctt cgcggcgcgg cttaactcaa gcgttagatg
    10261 cactaagcac ataattgctc acagccaaac tatcaggtca agtctgcttt tattattttt
    10321 aagcgtgcat aataagccct acacaaattg ggagatatat catgaaaggc tggctttttc
    10381 ttgttatcgc aatagttggc gaagtaatcg caacatagct tgcttggtcg ttccgcgtga
    10441 acgtcggctc gattgtacct gcgttcaaat actttgcgat cgtgttgcgc gcctgcccgg
    10501 tgcgtcggct gatctcacgg atcgactgct tctctcgcaa cgccatccga cggatgatgt
    10561 ttaaaagtcc catgtggatc actccgttgc cccgtcgctc accgtgttgg ggggaaggtg
    10621 cacatggctc agttctcaat ggaaattatc tgcctaaccg gctcagttct gcgtagaaac
    10681 caacatgcaa gctccaccgg gtgcaaagcg gcagcggc
//
