LOCUS B446p6oU10-ocs-L 10918 bp DNA circular SYN 22-JAN-2016 DEFINITION Ligation of pOCS-BM* into B433p6oU10-LH* ACCESSION B446p6oU10-ocs-L KEYWORDS . SOURCE Unknown. ORGANISM Unknown Unclassified. REFERENCE 1 (bases 1 to 10918) AUTHORS Self JOURNAL Unpublished. COMMENT SECID/File created by Clone Manager, Scientific & Educational Software COMMENT SECNOTES|Vector molecule: B433p6oU10-LH* Fragment ends: SfiI Fragment size: 10654 Insert molecule: pOCS-BM* Fragment ends: SfiI Fragment size: 264 Sequencing primers: SphR FEATURES Location/Qualifiers misc_feature 1..24 /gene="LB" /product="Left Border" /SECDrawAs="Region" /SECStyleId=1 misc_feature 309..1784 /gene="UBQ10" /product="PUbq10At" /SECDrawAs="Region" /SECStyleId=1 misc_feature 1413..1476 /product="Ubi cds 5" /SECDrawAs="Region" /SECStyleId=1 misc_feature 1479..1782 /gene="int" /SECDrawAs="Region" /SECStyleId=1 CDS 1804..2373 /gene="hptII" /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGR GYVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQD LPETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVY HWQTVMDDTVSASVAQALDELMLWAEDCPE" /SECDrawAs="Gene" /SECStyleId=1 /SECName="hptII" misc_feature 2374..2562 /gene="int" /product="Intron StLS1" /SECDrawAs="Gene" /SECStyleId=1 /SECName="int" /SECDescr="Intron StLS1" CDS 2563..3018 /gene="hptII" /codon_start=3 /SECDrawAs="Gene" /SECStyleId=1 misc_feature 3029..3319 /gene="TE9" /product="Terminator Rbcs-E9" /SECDrawAs="Region" /SECStyleId=1 misc_feature 3415..3610 /gene="Tocs" /product="Terminator" /SECDrawAs="Region" /SECStyleId=1 /SECName="Tocs" /SECDescr="Terminator" misc_feature 3695..3718 /gene="RB" /product="Right Border" /SECDrawAs="Region" /SECStyleId=1 misc_feature 4253..4950 /gene="ColE1" /product="ColE1 ORI" /SECDrawAs="Region" /SECStyleId=1 misc_feature 4987..5246 /gene="bom" /SECDrawAs="Region" /SECStyleId=1 misc_feature 5499..9204 /gene="pVS1" /product="ori for agrobacteria" /SECDrawAs="Region" /SECStyleId=1 CDS 9388..10398 /gene="Sm/Sp" /product="Strep/Spec resistence" /SECDrawAs="Gene" /SECStyleId=1 ORIGIN 1 ggcaggatat attcaattgt aaatggcttc atgtccggga aatctacatg gatcagcaat 61 gagtatgatg gtcaatatgg agaaaaagaa agagtaatta ccaatttttt ttcaattcaa 121 aaatgtagat gtccgcagcg ttattataaa atgaaagtac attttgataa aacgacaaat 181 tacgatccgt cgtatttata ggcgaaagca ataaacaaat tattctaatt cggaaatctt 241 tatttcgacg tgtctacatt cacgtccaaa tgggggctta gatgagaaac ttcacgatcg 301 gctctagatt tccttacatt ctgagcctct ttccttctaa tccactcatc tgcatcttct 361 tgtgtcctta ctaatacctc attggttcca aattccctcc ctttaagcac cagctcgttt 421 ctgttcttcc acagcctccc aagtatccaa gggactaaag cctccacatt cttcagatca 481 ggatattctt gtttaagatg ttgaactcta tggaggtttg tatgaactga tgatctagga 541 ccggataagt tcccttcttc atagcgaact tattcaaaga atgttttgtg tatcattctt 601 gttacattgt tattaatgaa aaaatattat tggtcattgg actgaacacg agtgttaaat 661 atggaccagg ccccaaataa gatccattga tatatgaatt aaataacaag aataaatcga 721 gtcaccaaac cacttgcctt ttttaacgag acttgttcac caacttgata caaaagtcat 781 tatcctatgc aaatcaataa tcatacaaaa atatccaata acactaaaaa attaaaagaa 841 atggataatt tcacaatatg ttatacgata aagaagttac ttttccaaga aattcactga 901 ttttataagc ccacttgcat tagataaatg gcaaaaaaaa acaaaaagga aaagaaataa 961 agcacgaaga attctagaaa atacgaaata cgcttcaatg cagtgggacc cacggttcaa 1021 ttattgccaa ttttcagctc caccgtatat ttaaaaaata aaacgataat gctaaaaaaa 1081 tataaatcgt aacgatcgtt aaatctcaac ggctggatct tatgacgacc gttagaaatt 1141 gtggttgtcg acgagtcagt aataaacggc gtcaaagtgg ttgcagccgg cacacacgag 1201 tcgtgtttat caactcaaag cacaaatact tttcctcaac ctaaaaataa ggcaattagc 1261 caaaaacaac tttgcgtgta aacaacgctc aatacacgtg tcattttatt attagctatt 1321 gcttcaccgc cttagctttc tcgtgaccta gtcgtcctcg tcttttcttc ttcttcttct 1381 ataaaacaat acccaaagag ctcttcttct tcacaattca gatttcaatt tctcaaaatc 1441 ttaaaaactt tctctcaatt ctctctaccg tgatcaaggt aaatttctgt gttccttatt 1501 ctctcaaaat cttcgatttt gttttcgttc gatcccaatt tcgtatatgt tctttggttt 1561 agattctgtt aatcttagat cgaagacgat tttctgggtt tgatcgttag atatcatctt 1621 aattctcgat tagggtttca tagatatcat ccgatttgtt caaataattt gagttttgtc 1681 gaataattac tcttcgattt gtgatttcta tctagatctg gtgttagttt ctagtttgtg 1741 cgatcgaatt tgtcgattaa tctgagtttt tctgattaac aggtactcga gtcctccata 1801 actatgaaga aaccggagct gaccgccact tccgtcgaaa agttcctcat cgagaagttt 1861 gatagcgtgt ctgaccttat gcagttgtcg gagggcgaag agtcccgcgc gttcagcttt 1921 gatgttggcg ggcgcgggta cgtcctccgc gtgaactctt gcgctgacgg cttctataaa 1981 gatcggtacg tttacaggca tttcgcctcg gcggccctgc ctattcccga agtcctcgac 2041 atcggcgagt tttccgagag ccttacgtat tgtatttccc gccgggctca aggggtgacc 2101 ctccaggatc tgccggaaac tgagttgcct gcggttctcc agcccgtcgc cgaggctatg 2161 gacgccatcg ccgcggcgga tctttctcaa acgtcgggct tcgggccgtt cggccctcag 2221 gggattggcc agtacaccac ttggagggac tttatctgcg ctatcgccga tccgcacgtg 2281 tatcattggc aaaccgttat ggacgatacg gtctccgcga gcgtggccca ggctctggac 2341 gaactcatgt tgtgggccga ggattgcccc gaggtaagtt tctgcttcta cctttgatat 2401 atatataata attatcatta attagtagta atataatatt tcaaatattt ttttcaaaat 2461 aaaagaatgt agtatatagc aattgctttt ctgtagttta taagtgtgta tattttaatt 2521 tataactttt ctaatatatg accaaaattt gttgatgtgc aggtccgcca cctggttcat 2581 gcggacttcg gctctaataa cgtgctcacc gataatgggc ggattactgc cgtcatcgac 2641 tggtccgaag ctatgtttgg cgattcgcag tacgaagtgg cgaacatttt cttctggagg 2701 ccttggcttg cctgcatgga acagcaaacg cgctactttg agcggaggca cccggagctg 2761 gcggggtctc cccgcctcag ggcctatatg ttgcggatcg gcctcgacca gctttaccag 2821 tccctggttg atgggaattt cgacgatgct gcctgggcgc aaggccgctg tgacgctatt 2881 gtcaggagcg gcgcggggac cgtgggccgc actcagatcg cccggcggtc ggctgccgtt 2941 tggacggatg ggtgcgtcga agtgctcgcg gacagcggca acaggcggcc ttccacccgc 3001 ccgcgggcca aggagtgaac gcgttcgagt attatggcat tgggaaaact gtttttcttg 3061 taccatttgt tgtgcttgta atttactgtg ttttttattc ggttttcgct atcgaactgt 3121 gaaatggaaa tggatggaga agagttaatg aatgatatgg tccttttgtt cattctcaaa 3181 ttaatattat ttgttttttc tcttatttgt tgtgtgttga atttgaaatt ataagagata 3241 tgcaaacatt ttgttttgag taaaaatgtg tcaaatcgtg gcctctaatg accgaagtta 3301 atatgaggag taaaacactg aagcctgcag gcatgcaagc tgatccacta gaggccatgg 3361 cggccgcact agtggatccc ccgggctgca ggaattcaag cttacgcgtg tcgaccctgc 3421 tttaatgaga tatgcgagac gcctatgatc gcatgatatt tgctttcaat tctgttgtgc 3481 acgttgtaaa aaacctgagc atgtgtagct cagatcctta ccgccggttt cggttcattc 3541 taatgaatat atcacccgtt actatcgtat ttttatgaat aatattctcc gttcaattta 3601 ctgattgtcc tcgacaggcc ttaagggcca gatcttgggc ccggtacccg atcagattgt 3661 cgtttcccgc cttcggttta aactatcagt gtttgacagg atatattggc gggtaaacct 3721 aagagaaaag agcgtttatt agaataatcg gatatttaaa agggcgtgaa aaggtttatc 3781 cgttcgtcca tttgtatgtg catgccaacc acagggttcc cctcgggagt gcttggcatt 3841 ccgtgcgata atgacttctg ttcaaccacc caaacgtcgg aaagcctgac gacggagcag 3901 cattccaaaa agatcccttg gctcgtctgg gtcggctaga aggtcgagtg ggctgctgtg 3961 gcttgatccc tcaacgcggt cgcggacgta gcgcagcgcc gaaaaatcct cgatcgcaaa 4021 tccgacgctg tcgaaaagcg tgatctgctt gtcgctcttt cggccgacgt cctggccagt 4081 catcacgcgc caaagttccg tcacaggatg atctggcgcg agttgctgga tctcgccttc 4141 aatccgggtc tgtggcggga actccacgaa aatatccgaa cgcagcaaga tatcgcggtg 4201 catctcggtc ttgcctgggc agtcgccgcc gacgccgttg atgtggacgc cgaaaaggat 4261 ctaggtgaag atcctttttg ataatctcat gaccaaaatc ccttaacgtg agttttcgtt 4321 ccactgagcg tcagaccccg tagaaaagat caaaggatct tcttgagatc ctttttttct 4381 gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc 4441 ggatcaagag ctaccaactc tttttccgaa ggtaactggc ttcagcagag cgcagatacc 4501 aaatactgtt cttctagtgt agccgtagtt aggccaccac ttcaagaact ctgtagcacc 4561 gcctacatac ctcgctctgc taatcctgtt accagtggct gctgccagtg gcgataagtc 4621 gtgtcttacc gggttggact caagacgata gttaccggat aaggcgcagc ggtcgggctg 4681 aacggggggt tcgtgcacac agcccagctt ggagcgaacg acctacaccg aactgagata 4741 cctacagcgt gagctatgag aaagcgccac gcttcccgaa gggagaaagg cggacaggta 4801 tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg gagcttccag ggggaaacgc 4861 ctggtatctt tatagtcctg tcgggtttcg ccacctctga cttgagcgtc gatttttgtg 4921 atgctcgtca ggggggcgga gcctatggaa aaacgccagc aacgcggcct ttttacggtt 4981 cctggccttt tgctggcctt ttgctcacat gttctttcct gcgttatccc ctgattctgt 5041 ggataaccga ttaccgcctt tgagtgagct gataccgctc gccgcagccg aacgaccgag 5101 cgcagcgagt cagtgagcga ggaagcggaa gagcgcctga tgcggtattt tctccttacg 5161 catctgtgcg gtatttcaca ccgcatatgg tgcactctca gtacaatctg ctctgatgcc 5221 gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc 5281 gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt 5341 acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac 5401 cgaaacgcgc gaggcagggg tacgtcgagg tcgatccaac ccctccgctg ctatagtgca 5461 gtcggcttct gacgttcagt gcagccgtct tctgaaaacg acatgtcgca caagtcctaa 5521 gttacgcgac aggctgccgc cctgcccttt tcctggcgtt ttcttgtcgc gtgttttagt 5581 cgcataaagt agaatacttg cgactagaac cggagacatt acgccatgaa caagagcgcc 5641 gccgctggcc tgctgggcta tgcccgcgtc agcaccgacg accaggactt gaccaaccaa 5701 cgggccgaac tgcacgcggc cggctgcacc aagctgtttt ccgagaagat caccggcacc 5761 aggcgcgacc gcccggagct ggccaggatg cttgaccacc tacgccctgg cgacgttgtg 5821 acagtgacca ggctagaccg cctggcccgc agcacccgcg acctactgga cattgccgag 5881 cgcatccagg aggccggcgc gggcctgcgt agcctggcag agccgtgggc cgacaccacc 5941 acgccggccg gccgcatggt gttgaccgtg ttcgccggca ttgccgagtt cgagcgttcc 6001 ctaatcatcg accgcacccg gagcgggcgc gaggccgcca aggcgcgagg cgtgaagttt 6061 ggcccccgcc ctaccctcac cccggcacag atcgcgcacg cccgcgagct gatcgaccag 6121 gaaggccgca ccgtgaaaga ggcggctgca ctgcttggcg tgcatcgctc gaccctgtac 6181 cgcgcacttg agcgcagcga ggaagtgacg cccaccgagg ccaggcggcg cggtgccttc 6241 cgtgaggacg cattgaccga ggccgacgcc ctggcggccg ccgagaatga acgccaagag 6301 gaacaagcat gaaaccgcac caggacggcc aggacgaacc gtttttcatt accgaagaga 6361 tcgaggcgga gatgatcgcg gccgggtacg tgttcgagcc gcccgcgcac gtctcaaccg 6421 tgcggctgca tgaaatcctg gccggtttgt ctgatgccaa gctcgcggcc tggccggcga 6481 gcttggccgc tgaagaaacc gagcgccgcc gtctaaaaag gtgatgtgta tttgagtaaa 6541 acagcttgcg tcatgcggtc gctgcgtata tgatgcgatg agtaaataaa caaatacgca 6601 aggggaacgc atgaaggtta tcgctgtact taaccagaaa ggcgggtcag gcaagacgac 6661 catcgcaacc catctagccc gcgccctgca actcgccggg gccgatgttc tgttagtcga 6721 ttccgatccc cagggcagtg cccgcgattg ggcggccgtg cgggaagatc aaccgctaac 6781 cgttgtcggc atcgaccgcc cgacgattga ccgcgacgtg aaggccatcg gccggcgcga 6841 cttcgtagtg atcgacggag cgccccaggc ggcggacttg gctgtgtccg cgatcaaggc 6901 agccgacttc gtgctgattc cggtgcagcc aagcccttac gacatatggg ccaccgccga 6961 cctggtggag ctggttaagc agcgcattga ggtcacggat ggaaggctac aagcggcctt 7021 tgtcgtgtcg cgggcgatca aaggcacgcg catcggcggt gaggttgccg aggcgctggc 7081 cgggtacgag ctgcccattc ttgagtcccg tatcacgcag cgcgtgagct acccaggcac 7141 tgccgccgcc ggcacaaccg ttcttgaatc agaacccgag ggcgacgctg cccgcgaggt 7201 ccaggcgctg gccgctgaaa ttaaatcaaa actcatttga gttaatgagg taaagagaaa 7261 atgagcaaaa gcacaaacac gctaagtgcc ggccgtccga gcgcacgcag cagcaaggct 7321 gcaacgttgg ccagcctggc agacacgcca gccatgaagc gggtcaactt tcagttgccg 7381 gcggaggatc acaccaagct gaagatgtac gcggtacgcc aaggcaagac cattaccgag 7441 ctgctatctg aatacatcgc gcagctacca gagtaaatga gcaaatgaat aaatgagtag 7501 atgaatttta gcggctaaag gaggcggcat ggaaaatcaa gaacaaccag gcaccgacgc 7561 cgtggaatgc cccatgtgtg gaggaacggg cggttggcca ggcgtaagcg gctgggttgt 7621 ctgccggccc tgcaatggca ctggaacccc caagcccgag gaatcggcgt gagcggtcgc 7681 aaaccatccg gcccggtaca aatcggcgcg gcgctgggtg atgacctggt ggagaagttg 7741 aaggcggcgc aggccgccca gcggcaacgc atcgaggcag aagcacgccc cggtgaatcg 7801 tggcaagcgg ccgctgatcg aatccgcaaa gaatcccggc aaccgccggc agccggtgcg 7861 ccgtcgatta ggaagccgcc caagggcgac gagcaaccag attttttcgt tccgatgctc 7921 tatgacgtgg gcacccgcga tagtcgcagc atcatggacg tggccgtttt ccgtctgtcg 7981 aagcgtgacc gacgagctgg cgaggtgatc cgctacgagc ttccagacgg gcacgtagag 8041 gtttccgcag ggccggccgg catggcgagt gtgtgggatt acgacctggt actgatggcg 8101 gtttcccatc taaccgaatc catgaaccga taccgggaag ggaagggaga caagcccggc 8161 cgcgtgttcc gtccacacgt tgcggacgta ctcaagttct gccggcgagc cgatggcgga 8221 aagcagaaag acgacctggt agaaacctgc attcggttaa acaccacgca cgttgccatg 8281 cagcgtacga agaaggccaa gaacggccgc ctggtgacgg tatccgaggg tgaagccttg 8341 attagccgct acaagatcgt aaagagcgaa accgggcggc cggagtacat cgagatcgag 8401 ctagctgatt ggatgtaccg cgagatcaca gaaggcaaga acccggacgt gctgacggtt 8461 caccccgatt actttttgat cgatcccggc atcggccgtt ttctctaccg cctggcacgc 8521 cgcgccgcag gcaaggcaga agccagatgg ttgttcaaga cgatctacga acgcagtggc 8581 agcgccggag agttcaagaa gttctgtttc accgtgcgca agctgatcgg gtcaaatgac 8641 ctgccggagt acgatttgaa ggaggaggcg gggcaggctg gcccgatcct agtcatgcgc 8701 taccgcaacc tgatcgaggg cgaagcatcc gccggttcct aatgtacgga gcagatgcta 8761 gggcaaattg ccctagcagg ggaaaaaggt cgaaaaggtc tctttcctgt ggatagcacg 8821 tacattggga acccaaagcc gtacattggg aaccggaacc cgtacattgg gaacccaaag 8881 ccgtacattg ggaaccggtc acacatgtaa gtgactgata taaaagagaa aaaaggcgat 8941 ttttccgcct aaaactcttt aaaacttatt aaaactctta aaacccgcct ggcctgtgca 9001 taactgtctg gccagcgcac agccgaagag ctgcaaaaag cgcctaccct tcggtcgctg 9061 cgctccctac gccccgccgc ttcgcgtcgg cctatcgcgg ccgctggccg ctcaaaaatg 9121 gctggcctac ggccaggcaa tctaccaggg cgcggacaag ccgcgccgtc gccactcgac 9181 cgccggcgcc cacatcaagg caccggtggg tatgcctgac gatgcgtgga gaccgaaacc 9241 ttgcgctcgt tcgccagcca ggacagaaat gcctcgactt cgctgctgcc caaggttgcc 9301 gggtgacgca caccgtggaa acggatgaag gcacgaaccc agtggacata agcctgttcg 9361 gttcgtaagc tgtaatgcaa gtagcgtatg cgctcacgca actggtccag aaccttgacc 9421 gaacgcagcg gtggtaacgg cgcagtggcg gttttcatgg cttgttatga ctgttttttt 9481 ggggtacagt ctatgcctcg ggcatccaag cagcaagcgc gttacgccgt gggtcgatgt 9541 ttgatgttat ggagcagcaa cgatgttacg cagcagggca gtcgccctaa aacaaagtta 9601 aacatcatga gggaagcggt gatcgccgaa gtatcgactc aactatcaga ggtagttggc 9661 gtcatcgagc gccatctcga accgacgttg ctggccgtac atttgtacgg ctccgcagtg 9721 gatggcggcc tgaagccaca cagtgatatt gatttgctgg ttacggtgac cgtaaggctt 9781 gatgaaacaa cgcggcgagc tttgatcaac gaccttttgg aaacttcggc ttcccctgga 9841 gagagcgaga ttctccgcgc tgtagaagtc accattgttg tgcacgacga catcattccg 9901 tggcgttatc cagctaagcg cgaactgcaa tttggagaat ggcagcgcaa tgacattctt 9961 gcaggtatct tcgagccagc cacgatcgac attgatctgg ctatcttgct gacaaaagca 10021 agagaacata gcgttgcctt ggtaggtcca gcggcggagg aactctttga tccggttcct 10081 gaacaggatc tatttgaggc gctaaatgaa accttaacgc tatggaactc gccgcccgac 10141 tgggctggcg atgagcgaaa tgtagtgctt acgttgtccc gcatttggta cagcgcagta 10201 accggcaaaa tcgcgccgaa ggatgtcgct gccgactggg caatggagcg cctgccggcc 10261 cagtatcagc ccgtcatact tgaagctaga caggcttatc ttggacaaga agaagatcgc 10321 ttggcctcgc gcgcagatca gttggaagaa tttgtccact acgtgaaagg cgagatcacc 10381 aaggtagtcg gcaaataatg tctaacaatt cgttcaagcc gacgccgctt cgcggcgcgg 10441 cttaactcaa gcgttagatg cactaagcac ataattgctc acagccaaac tatcaggtca 10501 agtctgcttt tattattttt aagcgtgcat aataagccct acacaaattg ggagatatat 10561 catgaaaggc tggctttttc ttgttatcgc aatagttggc gaagtaatcg caacatagct 10621 tgcttggtcg ttccgcgtga acgtcggctc gattgtacct gcgttcaaat actttgcgat 10681 cgtgttgcgc gcctgcccgg tgcgtcggct gatctcacgg atcgactgct tctctcgcaa 10741 cgccatccga cggatgatgt ttaaaagtcc catgtggatc actccgttgc cccgtcgctc 10801 accgtgttgg ggggaaggtg cacatggctc agttctcaat ggaaattatc tgcctaaccg 10861 gctcagttct gcgtagaaac caacatgcaa gctccaccgg gtgcaaagcg gcagcggc //