LOCUS       B448p9oU10-ocs-L       10696 bp    DNA     circular SYN 22-JAN-2016
DEFINITION  Ligation of pOCS-BM* into B435p9oU10-LH*
ACCESSION   B448p9oU10-ocs-L
KEYWORDS    .
SOURCE      Unknown.
  ORGANISM  Unknown
            Unclassified.
REFERENCE   1  (bases 1 to 10696)
  AUTHORS   Self
  JOURNAL   Unpublished.
COMMENT     SECID/File created by Clone Manager, Scientific & Educational Software
COMMENT     SECNOTES|Vector molecule:  B435p9oU10-LH*
               Fragment ends: SfiI
               Fragment size: 10432
            Insert molecule:  pOCS-BM*
               Fragment ends: SfiI
               Fragment size: 264
            Sequencing primers	
            SphR
FEATURES             Location/Qualifiers
     misc_feature    1..24
                     /gene="LB"
                     /product="Left Border"
                     /SECDrawAs="Region"
                     /SECStyleId=1
     misc_feature    309..1784
                     /gene="UBQ10"
                     /product="PUbq10At"
                     /SECDrawAs="Region"
                     /SECStyleId=1
     misc_feature    1413..1476
                     /product="Ubi cds 5"
                     /SECDrawAs="Region"
                     /SECStyleId=1
     misc_feature    1479..1782
                     /gene="int"
                     /SECDrawAs="Region"
                     /SECStyleId=1
     CDS             1804..2102
                     /gene="nptIIo"
                     /product="NptII optimiert"
                     /SECDrawAs="Gene"
                     /SECStyleId=1
     misc_signal     2099..2102
                     /label=splice
                     /SECDrawAs="Label"
     CDS             2103..2289
                     /gene="int"
                     /SECDrawAs="Gene"
                     /SECStyleId=1
     misc_signal     2288..2291
                     /label=splice
                     /SECDrawAs="Label"
     CDS             2290..2793
                     /gene="nptIIo"
                     /product="NptII optimiert"
                     /SECDrawAs="Gene"
                     /SECStyleId=1
     misc_feature    2807..3097
                     /gene="TE9"
                     /product="Terminator Rbcs-E9"
                     /SECDrawAs="Region"
                     /SECStyleId=1
     misc_feature    3193..3388
                     /gene="Tocs"
                     /product="Terminator"
                     /SECDrawAs="Region"
                     /SECStyleId=1
                     /SECName="Tocs"
                     /SECDescr="Terminator"
     misc_feature    3473..3496
                     /gene="RB"
                     /product="Right Border"
                     /SECDrawAs="Region"
                     /SECStyleId=1
     misc_feature    4031..4728
                     /gene="ColE1"
                     /product="ColE1 ORI"
                     /SECDrawAs="Region"
                     /SECStyleId=1
     misc_feature    4765..5024
                     /gene="bom"
                     /SECDrawAs="Region"
                     /SECStyleId=1
     misc_feature    5277..8982
                     /gene="pVS1"
                     /product="ori for agrobacteria"
                     /SECDrawAs="Region"
                     /SECStyleId=1
     CDS             9166..10176
                     /gene="Sm/Sp"
                     /product="Strep/Spec resistence"
                     /SECDrawAs="Gene"
                     /SECStyleId=1
ORIGIN      
        1 ggcaggatat attcaattgt aaatggcttc atgtccggga aatctacatg gatcagcaat
       61 gagtatgatg gtcaatatgg agaaaaagaa agagtaatta ccaatttttt ttcaattcaa
      121 aaatgtagat gtccgcagcg ttattataaa atgaaagtac attttgataa aacgacaaat
      181 tacgatccgt cgtatttata ggcgaaagca ataaacaaat tattctaatt cggaaatctt
      241 tatttcgacg tgtctacatt cacgtccaaa tgggggctta gatgagaaac ttcacgatcg
      301 gctctagatt tccttacatt ctgagcctct ttccttctaa tccactcatc tgcatcttct
      361 tgtgtcctta ctaatacctc attggttcca aattccctcc ctttaagcac cagctcgttt
      421 ctgttcttcc acagcctccc aagtatccaa gggactaaag cctccacatt cttcagatca
      481 ggatattctt gtttaagatg ttgaactcta tggaggtttg tatgaactga tgatctagga
      541 ccggataagt tcccttcttc atagcgaact tattcaaaga atgttttgtg tatcattctt
      601 gttacattgt tattaatgaa aaaatattat tggtcattgg actgaacacg agtgttaaat
      661 atggaccagg ccccaaataa gatccattga tatatgaatt aaataacaag aataaatcga
      721 gtcaccaaac cacttgcctt ttttaacgag acttgttcac caacttgata caaaagtcat
      781 tatcctatgc aaatcaataa tcatacaaaa atatccaata acactaaaaa attaaaagaa
      841 atggataatt tcacaatatg ttatacgata aagaagttac ttttccaaga aattcactga
      901 ttttataagc ccacttgcat tagataaatg gcaaaaaaaa acaaaaagga aaagaaataa
      961 agcacgaaga attctagaaa atacgaaata cgcttcaatg cagtgggacc cacggttcaa
     1021 ttattgccaa ttttcagctc caccgtatat ttaaaaaata aaacgataat gctaaaaaaa
     1081 tataaatcgt aacgatcgtt aaatctcaac ggctggatct tatgacgacc gttagaaatt
     1141 gtggttgtcg acgagtcagt aataaacggc gtcaaagtgg ttgcagccgg cacacacgag
     1201 tcgtgtttat caactcaaag cacaaatact tttcctcaac ctaaaaataa ggcaattagc
     1261 caaaaacaac tttgcgtgta aacaacgctc aatacacgtg tcattttatt attagctatt
     1321 gcttcaccgc cttagctttc tcgtgaccta gtcgtcctcg tcttttcttc ttcttcttct
     1381 ataaaacaat acccaaagag ctcttcttct tcacaattca gatttcaatt tctcaaaatc
     1441 ttaaaaactt tctctcaatt ctctctaccg tgatcaaggt aaatttctgt gttccttatt
     1501 ctctcaaaat cttcgatttt gttttcgttc gatcccaatt tcgtatatgt tctttggttt
     1561 agattctgtt aatcttagat cgaagacgat tttctgggtt tgatcgttag atatcatctt
     1621 aattctcgat tagggtttca tagatatcat ccgatttgtt caaataattt gagttttgtc
     1681 gaataattac tcttcgattt gtgatttcta tctagatctg gtgttagttt ctagtttgtg
     1741 cgatcgaatt tgtcgattaa tctgagtttt tctgattaac aggtactcga gtcctccata
     1801 actatgggag catggattga gcaggatggc ctccatgctg gtagccccgc tgcttgggtg
     1861 gagcgcctct ttggctacga ctgggcccaa cagaccattg ggtgcagtga tgccgctgtc
     1921 ttccgtttga gcgcccaggg caggccagtt ctcttcgtga agactgacct ctccggtgcc
     1981 ctcaacgagt tgcaggacga agctgcccgc ctctcatggc ttgccaccac cggagtccct
     2041 tgcgctgccg tgttggacgt tgtcacagag gccggaagag actggctgct cttgggcgag
     2101 gtaagtttct gcttctacct ttgatatata tataataatt atcattaatt agtagtaata
     2161 taatatttca aatatttttt tcaaaataaa agaatgtagt atatagcaat tgcttttctg
     2221 tagtttataa gtgtgtatat tttaatttat aacttttcta atatatgacc aaaatttgtt
     2281 gatgtgcagg tgccaggcca agacctcctg agctcccacc tggccccagc cgagaaggtc
     2341 tccatcatgg cagacgccat gcgtcgcctc cacaccctgg acccagccac ctgccccttc
     2401 gaccaccagg ccaagcacag gatcgaacgt gcacgcacta ggatggaggc cggccttgtg
     2461 gaccaggatg accttgacga ggagcaccag ggccttgctc ccgccgagct gttcgcccgc
     2521 ctcaaggcta gaatgcctga cggagaggac ttggtggtca cccacgggga tgcctgcctc
     2581 cccaacatca tggttgagaa cggacgcttc tctggcttca tcgactgcgg tcgtctgggc
     2641 gtggccgacc gctaccaaga catcgccctg gccaccaggg acatcgctga ggaacttgga
     2701 ggggagtggg ccgatcgttt cttggtcctc tacggcattg ctgcccccga ctcccagagg
     2761 atcgccttct accgcctgct tgacgagttc ttctgaacgc gttcgagtat tatggcattg
     2821 ggaaaactgt ttttcttgta ccatttgttg tgcttgtaat ttactgtgtt ttttattcgg
     2881 ttttcgctat cgaactgtga aatggaaatg gatggagaag agttaatgaa tgatatggtc
     2941 cttttgttca ttctcaaatt aatattattt gttttttctc ttatttgttg tgtgttgaat
     3001 ttgaaattat aagagatatg caaacatttt gttttgagta aaaatgtgtc aaatcgtggc
     3061 ctctaatgac cgaagttaat atgaggagta aaacactgaa gcctgcaggc atgcaagctg
     3121 atccactaga ggccatggcg gccgcactag tggatccccc gggctgcagg aattcaagct
     3181 tacgcgtgtc gaccctgctt taatgagata tgcgagacgc ctatgatcgc atgatatttg
     3241 ctttcaattc tgttgtgcac gttgtaaaaa acctgagcat gtgtagctca gatccttacc
     3301 gccggtttcg gttcattcta atgaatatat cacccgttac tatcgtattt ttatgaataa
     3361 tattctccgt tcaatttact gattgtcctc gacaggcctt aagggccaga tcttgggccc
     3421 ggtacccgat cagattgtcg tttcccgcct tcggtttaaa ctatcagtgt ttgacaggat
     3481 atattggcgg gtaaacctaa gagaaaagag cgtttattag aataatcgga tatttaaaag
     3541 ggcgtgaaaa ggtttatccg ttcgtccatt tgtatgtgca tgccaaccac agggttcccc
     3601 tcgggagtgc ttggcattcc gtgcgataat gacttctgtt caaccaccca aacgtcggaa
     3661 agcctgacga cggagcagca ttccaaaaag atcccttggc tcgtctgggt cggctagaag
     3721 gtcgagtggg ctgctgtggc ttgatccctc aacgcggtcg cggacgtagc gcagcgccga
     3781 aaaatcctcg atcgcaaatc cgacgctgtc gaaaagcgtg atctgcttgt cgctctttcg
     3841 gccgacgtcc tggccagtca tcacgcgcca aagttccgtc acaggatgat ctggcgcgag
     3901 ttgctggatc tcgccttcaa tccgggtctg tggcgggaac tccacgaaaa tatccgaacg
     3961 cagcaagata tcgcggtgca tctcggtctt gcctgggcag tcgccgccga cgccgttgat
     4021 gtggacgccg aaaaggatct aggtgaagat cctttttgat aatctcatga ccaaaatccc
     4081 ttaacgtgag ttttcgttcc actgagcgtc agaccccgta gaaaagatca aaggatcttc
     4141 ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc
     4201 agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt
     4261 cagcagagcg cagataccaa atactgttct tctagtgtag ccgtagttag gccaccactt
     4321 caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc
     4381 tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa
     4441 ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac
     4501 ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg
     4561 gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga
     4621 gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact
     4681 tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa
     4741 cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc
     4801 gttatcccct gattctgtgg ataaccgatt accgcctttg agtgagctga taccgctcgc
     4861 cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg
     4921 cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatggtg cactctcagt
     4981 acaatctgct ctgatgccgc atagttaagc cagtatacac tccgctatcg ctacgtgact
     5041 gggtcatggc tgcgccccga cacccgccaa cacccgctga cgcgccctga cgggcttgtc
     5101 tgctcccggc atccgcttac agacaagctg tgaccgtctc cgggagctgc atgtgtcaga
     5161 ggttttcacc gtcatcaccg aaacgcgcga ggcaggggta cgtcgaggtc gatccaaccc
     5221 ctccgctgct atagtgcagt cggcttctga cgttcagtgc agccgtcttc tgaaaacgac
     5281 atgtcgcaca agtcctaagt tacgcgacag gctgccgccc tgcccttttc ctggcgtttt
     5341 cttgtcgcgt gttttagtcg cataaagtag aatacttgcg actagaaccg gagacattac
     5401 gccatgaaca agagcgccgc cgctggcctg ctgggctatg cccgcgtcag caccgacgac
     5461 caggacttga ccaaccaacg ggccgaactg cacgcggccg gctgcaccaa gctgttttcc
     5521 gagaagatca ccggcaccag gcgcgaccgc ccggagctgg ccaggatgct tgaccaccta
     5581 cgccctggcg acgttgtgac agtgaccagg ctagaccgcc tggcccgcag cacccgcgac
     5641 ctactggaca ttgccgagcg catccaggag gccggcgcgg gcctgcgtag cctggcagag
     5701 ccgtgggccg acaccaccac gccggccggc cgcatggtgt tgaccgtgtt cgccggcatt
     5761 gccgagttcg agcgttccct aatcatcgac cgcacccgga gcgggcgcga ggccgccaag
     5821 gcgcgaggcg tgaagtttgg cccccgccct accctcaccc cggcacagat cgcgcacgcc
     5881 cgcgagctga tcgaccagga aggccgcacc gtgaaagagg cggctgcact gcttggcgtg
     5941 catcgctcga ccctgtaccg cgcacttgag cgcagcgagg aagtgacgcc caccgaggcc
     6001 aggcggcgcg gtgccttccg tgaggacgca ttgaccgagg ccgacgccct ggcggccgcc
     6061 gagaatgaac gccaagagga acaagcatga aaccgcacca ggacggccag gacgaaccgt
     6121 ttttcattac cgaagagatc gaggcggaga tgatcgcggc cgggtacgtg ttcgagccgc
     6181 ccgcgcacgt ctcaaccgtg cggctgcatg aaatcctggc cggtttgtct gatgccaagc
     6241 tcgcggcctg gccggcgagc ttggccgctg aagaaaccga gcgccgccgt ctaaaaaggt
     6301 gatgtgtatt tgagtaaaac agcttgcgtc atgcggtcgc tgcgtatatg atgcgatgag
     6361 taaataaaca aatacgcaag gggaacgcat gaaggttatc gctgtactta accagaaagg
     6421 cgggtcaggc aagacgacca tcgcaaccca tctagcccgc gccctgcaac tcgccggggc
     6481 cgatgttctg ttagtcgatt ccgatcccca gggcagtgcc cgcgattggg cggccgtgcg
     6541 ggaagatcaa ccgctaaccg ttgtcggcat cgaccgcccg acgattgacc gcgacgtgaa
     6601 ggccatcggc cggcgcgact tcgtagtgat cgacggagcg ccccaggcgg cggacttggc
     6661 tgtgtccgcg atcaaggcag ccgacttcgt gctgattccg gtgcagccaa gcccttacga
     6721 catatgggcc accgccgacc tggtggagct ggttaagcag cgcattgagg tcacggatgg
     6781 aaggctacaa gcggcctttg tcgtgtcgcg ggcgatcaaa ggcacgcgca tcggcggtga
     6841 ggttgccgag gcgctggccg ggtacgagct gcccattctt gagtcccgta tcacgcagcg
     6901 cgtgagctac ccaggcactg ccgccgccgg cacaaccgtt cttgaatcag aacccgaggg
     6961 cgacgctgcc cgcgaggtcc aggcgctggc cgctgaaatt aaatcaaaac tcatttgagt
     7021 taatgaggta aagagaaaat gagcaaaagc acaaacacgc taagtgccgg ccgtccgagc
     7081 gcacgcagca gcaaggctgc aacgttggcc agcctggcag acacgccagc catgaagcgg
     7141 gtcaactttc agttgccggc ggaggatcac accaagctga agatgtacgc ggtacgccaa
     7201 ggcaagacca ttaccgagct gctatctgaa tacatcgcgc agctaccaga gtaaatgagc
     7261 aaatgaataa atgagtagat gaattttagc ggctaaagga ggcggcatgg aaaatcaaga
     7321 acaaccaggc accgacgccg tggaatgccc catgtgtgga ggaacgggcg gttggccagg
     7381 cgtaagcggc tgggttgtct gccggccctg caatggcact ggaaccccca agcccgagga
     7441 atcggcgtga gcggtcgcaa accatccggc ccggtacaaa tcggcgcggc gctgggtgat
     7501 gacctggtgg agaagttgaa ggcggcgcag gccgcccagc ggcaacgcat cgaggcagaa
     7561 gcacgccccg gtgaatcgtg gcaagcggcc gctgatcgaa tccgcaaaga atcccggcaa
     7621 ccgccggcag ccggtgcgcc gtcgattagg aagccgccca agggcgacga gcaaccagat
     7681 tttttcgttc cgatgctcta tgacgtgggc acccgcgata gtcgcagcat catggacgtg
     7741 gccgttttcc gtctgtcgaa gcgtgaccga cgagctggcg aggtgatccg ctacgagctt
     7801 ccagacgggc acgtagaggt ttccgcaggg ccggccggca tggcgagtgt gtgggattac
     7861 gacctggtac tgatggcggt ttcccatcta accgaatcca tgaaccgata ccgggaaggg
     7921 aagggagaca agcccggccg cgtgttccgt ccacacgttg cggacgtact caagttctgc
     7981 cggcgagccg atggcggaaa gcagaaagac gacctggtag aaacctgcat tcggttaaac
     8041 accacgcacg ttgccatgca gcgtacgaag aaggccaaga acggccgcct ggtgacggta
     8101 tccgagggtg aagccttgat tagccgctac aagatcgtaa agagcgaaac cgggcggccg
     8161 gagtacatcg agatcgagct agctgattgg atgtaccgcg agatcacaga aggcaagaac
     8221 ccggacgtgc tgacggttca ccccgattac tttttgatcg atcccggcat cggccgtttt
     8281 ctctaccgcc tggcacgccg cgccgcaggc aaggcagaag ccagatggtt gttcaagacg
     8341 atctacgaac gcagtggcag cgccggagag ttcaagaagt tctgtttcac cgtgcgcaag
     8401 ctgatcgggt caaatgacct gccggagtac gatttgaagg aggaggcggg gcaggctggc
     8461 ccgatcctag tcatgcgcta ccgcaacctg atcgagggcg aagcatccgc cggttcctaa
     8521 tgtacggagc agatgctagg gcaaattgcc ctagcagggg aaaaaggtcg aaaaggtctc
     8581 tttcctgtgg atagcacgta cattgggaac ccaaagccgt acattgggaa ccggaacccg
     8641 tacattggga acccaaagcc gtacattggg aaccggtcac acatgtaagt gactgatata
     8701 aaagagaaaa aaggcgattt ttccgcctaa aactctttaa aacttattaa aactcttaaa
     8761 acccgcctgg cctgtgcata actgtctggc cagcgcacag ccgaagagct gcaaaaagcg
     8821 cctacccttc ggtcgctgcg ctccctacgc cccgccgctt cgcgtcggcc tatcgcggcc
     8881 gctggccgct caaaaatggc tggcctacgg ccaggcaatc taccagggcg cggacaagcc
     8941 gcgccgtcgc cactcgaccg ccggcgccca catcaaggca ccggtgggta tgcctgacga
     9001 tgcgtggaga ccgaaacctt gcgctcgttc gccagccagg acagaaatgc ctcgacttcg
     9061 ctgctgccca aggttgccgg gtgacgcaca ccgtggaaac ggatgaaggc acgaacccag
     9121 tggacataag cctgttcggt tcgtaagctg taatgcaagt agcgtatgcg ctcacgcaac
     9181 tggtccagaa ccttgaccga acgcagcggt ggtaacggcg cagtggcggt tttcatggct
     9241 tgttatgact gtttttttgg ggtacagtct atgcctcggg catccaagca gcaagcgcgt
     9301 tacgccgtgg gtcgatgttt gatgttatgg agcagcaacg atgttacgca gcagggcagt
     9361 cgccctaaaa caaagttaaa catcatgagg gaagcggtga tcgccgaagt atcgactcaa
     9421 ctatcagagg tagttggcgt catcgagcgc catctcgaac cgacgttgct ggccgtacat
     9481 ttgtacggct ccgcagtgga tggcggcctg aagccacaca gtgatattga tttgctggtt
     9541 acggtgaccg taaggcttga tgaaacaacg cggcgagctt tgatcaacga ccttttggaa
     9601 acttcggctt cccctggaga gagcgagatt ctccgcgctg tagaagtcac cattgttgtg
     9661 cacgacgaca tcattccgtg gcgttatcca gctaagcgcg aactgcaatt tggagaatgg
     9721 cagcgcaatg acattcttgc aggtatcttc gagccagcca cgatcgacat tgatctggct
     9781 atcttgctga caaaagcaag agaacatagc gttgccttgg taggtccagc ggcggaggaa
     9841 ctctttgatc cggttcctga acaggatcta tttgaggcgc taaatgaaac cttaacgcta
     9901 tggaactcgc cgcccgactg ggctggcgat gagcgaaatg tagtgcttac gttgtcccgc
     9961 atttggtaca gcgcagtaac cggcaaaatc gcgccgaagg atgtcgctgc cgactgggca
    10021 atggagcgcc tgccggccca gtatcagccc gtcatacttg aagctagaca ggcttatctt
    10081 ggacaagaag aagatcgctt ggcctcgcgc gcagatcagt tggaagaatt tgtccactac
    10141 gtgaaaggcg agatcaccaa ggtagtcggc aaataatgtc taacaattcg ttcaagccga
    10201 cgccgcttcg cggcgcggct taactcaagc gttagatgca ctaagcacat aattgctcac
    10261 agccaaacta tcaggtcaag tctgctttta ttatttttaa gcgtgcataa taagccctac
    10321 acaaattggg agatatatca tgaaaggctg gctttttctt gttatcgcaa tagttggcga
    10381 agtaatcgca acatagcttg cttggtcgtt ccgcgtgaac gtcggctcga ttgtacctgc
    10441 gttcaaatac tttgcgatcg tgttgcgcgc ctgcccggtg cgtcggctga tctcacggat
    10501 cgactgcttc tctcgcaacg ccatccgacg gatgatgttt aaaagtccca tgtggatcac
    10561 tccgttgccc cgtcgctcac cgtgttgggg ggaaggtgca catggctcag ttctcaatgg
    10621 aaattatctg cctaaccggc tcagttctgc gtagaaacca acatgcaagc tccaccgggt
    10681 gcaaagcggc agcggc
//
