LOCUS       B467p7oU10-35s-o       10872 bp    DNA     circular SYN 08-FEB-2016
DEFINITION  Ligation of p35-OCS-BM* into B434p7oU10-LH*
ACCESSION   B467p7oU10-35s-o
KEYWORDS    .
SOURCE      Unknown.
  ORGANISM  Unknown
            Unclassified.
REFERENCE   1  (bases 1 to 10872)
  AUTHORS   Self
  JOURNAL   Unpublished.
COMMENT     SECID/File created by Clone Manager, Scientific & Educational Software
COMMENT     SECNOTES|Vector molecule:  B434p7oU10-LH*
               Fragment ends: SfiI
               Fragment size: 10180
            Insert molecule:  p35-OCS-BM*
               Fragment ends: SfiI
               Fragment size: 692
            Sequencing primers	
            SphR	GGGTGGTTGAACAGAAGTCAT
FEATURES             Location/Qualifiers
     misc_feature    1..24
                     /gene="LB"
                     /product="Left Border"
                     /SECDrawAs="Region"
                     /SECStyleId=1
     misc_feature    309..1784
                     /gene="UBQ10"
                     /product="PUbq10At"
                     /SECDrawAs="Region"
                     /SECStyleId=1
     misc_feature    1413..1476
                     /product="Ubi cds 5"
                     /SECDrawAs="Region"
                     /SECStyleId=1
     misc_feature    1479..1782
                     /gene="int"
                     /SECDrawAs="Region"
                     /SECStyleId=1
     CDS             1804..2037
                     /gene="bar"
                     /SECDrawAs="Gene"
                     /SECStyleId=1
     misc_feature    2038..2226
                     /gene="int"
                     /product="STLS1"
                     /SECDrawAs="Gene"
                     /SECStyleId=1
                     /SECName="int"
                     /SECDescr="STLS1"
     CDS             2227..2542
                     /gene="bar"
                     /SECDrawAs="Gene"
                     /SECStyleId=1
     misc_feature    2555..2845
                     /gene="TE9"
                     /product="Terminator Rbcs-E9"
                     /SECDrawAs="Region"
                     /SECStyleId=1
     misc_feature    2900..3322
                     /gene="P35S"
                     /product="Promotor-Region"
                     /SECDrawAs="Region"
                     /SECStyleId=1
                     /SECName="P35S"
                     /SECDescr="Promotor-Region"
     misc_feature    3369..3564
                     /gene="Tocs"
                     /product="Terminator"
                     /SECDrawAs="Region"
                     /SECStyleId=1
                     /SECName="Tocs"
                     /SECDescr="Terminator"
     misc_feature    3649..3672
                     /gene="RB"
                     /product="Right Border"
                     /SECDrawAs="Region"
                     /SECStyleId=1
     misc_feature    4207..4904
                     /gene="ColE1"
                     /product="ColE1 ORI"
                     /SECDrawAs="Region"
                     /SECStyleId=1
     misc_feature    4941..5200
                     /gene="bom"
                     /SECDrawAs="Region"
                     /SECStyleId=1
     misc_feature    5453..9158
                     /gene="pVS1"
                     /product="ori for agrobacteria"
                     /SECDrawAs="Region"
                     /SECStyleId=1
     CDS             9342..10352
                     /gene="Sm/Sp"
                     /product="Strep/Spec resistence"
                     /SECDrawAs="Gene"
                     /SECStyleId=1
ORIGIN      
        1 ggcaggatat attcaattgt aaatggcttc atgtccggga aatctacatg gatcagcaat
       61 gagtatgatg gtcaatatgg agaaaaagaa agagtaatta ccaatttttt ttcaattcaa
      121 aaatgtagat gtccgcagcg ttattataaa atgaaagtac attttgataa aacgacaaat
      181 tacgatccgt cgtatttata ggcgaaagca ataaacaaat tattctaatt cggaaatctt
      241 tatttcgacg tgtctacatt cacgtccaaa tgggggctta gatgagaaac ttcacgatcg
      301 gctctagatt tccttacatt ctgagcctct ttccttctaa tccactcatc tgcatcttct
      361 tgtgtcctta ctaatacctc attggttcca aattccctcc ctttaagcac cagctcgttt
      421 ctgttcttcc acagcctccc aagtatccaa gggactaaag cctccacatt cttcagatca
      481 ggatattctt gtttaagatg ttgaactcta tggaggtttg tatgaactga tgatctagga
      541 ccggataagt tcccttcttc atagcgaact tattcaaaga atgttttgtg tatcattctt
      601 gttacattgt tattaatgaa aaaatattat tggtcattgg actgaacacg agtgttaaat
      661 atggaccagg ccccaaataa gatccattga tatatgaatt aaataacaag aataaatcga
      721 gtcaccaaac cacttgcctt ttttaacgag acttgttcac caacttgata caaaagtcat
      781 tatcctatgc aaatcaataa tcatacaaaa atatccaata acactaaaaa attaaaagaa
      841 atggataatt tcacaatatg ttatacgata aagaagttac ttttccaaga aattcactga
      901 ttttataagc ccacttgcat tagataaatg gcaaaaaaaa acaaaaagga aaagaaataa
      961 agcacgaaga attctagaaa atacgaaata cgcttcaatg cagtgggacc cacggttcaa
     1021 ttattgccaa ttttcagctc caccgtatat ttaaaaaata aaacgataat gctaaaaaaa
     1081 tataaatcgt aacgatcgtt aaatctcaac ggctggatct tatgacgacc gttagaaatt
     1141 gtggttgtcg acgagtcagt aataaacggc gtcaaagtgg ttgcagccgg cacacacgag
     1201 tcgtgtttat caactcaaag cacaaatact tttcctcaac ctaaaaataa ggcaattagc
     1261 caaaaacaac tttgcgtgta aacaacgctc aatacacgtg tcattttatt attagctatt
     1321 gcttcaccgc cttagctttc tcgtgaccta gtcgtcctcg tcttttcttc ttcttcttct
     1381 ataaaacaat acccaaagag ctcttcttct tcacaattca gatttcaatt tctcaaaatc
     1441 ttaaaaactt tctctcaatt ctctctaccg tgatcaaggt aaatttctgt gttccttatt
     1501 ctctcaaaat cttcgatttt gttttcgttc gatcccaatt tcgtatatgt tctttggttt
     1561 agattctgtt aatcttagat cgaagacgat tttctgggtt tgatcgttag atatcatctt
     1621 aattctcgat tagggtttca tagatatcat ccgatttgtt caaataattt gagttttgtc
     1681 gaataattac tcttcgattt gtgatttcta tctagatctg gtgttagttt ctagtttgtg
     1741 cgatcgaatt tgtcgattaa tctgagtttt tctgattaac aggtactcga gtcctccata
     1801 actatgggcc ccgagcgcag gcccgccgac atccgccgtg ccaccgaggc cgacatgcca
     1861 gccgtctgca ccatcgtgaa ccactacatc gagacctcca cggtcaactt ccgcacagag
     1921 ccgcaggagc ctcaggagtg gaccgacgac ctcgtgaggc tgcgcgagcg ctacccctgg
     1981 ctcgtcgctg aggtggatgg cgaggtcgcc ggcatcgcct acgcgggccc ctggaaggta
     2041 agtttctgct tctacctttg atatatatat aataattatc attaattagt agtaatataa
     2101 tatttcaaat atttttttca aaataaaaga atgtagtata tagcaattgc ttttctgtag
     2161 tttataagtg tgtatatttt aatttataac ttttctaata tatgaccaaa atttgttgat
     2221 gtgcaggcac gcaacgccta cgactggacg gcggagagca ccgtctacgt gtccccacgc
     2281 caccagcgca ccggcctggg ctccacgctc tacacccacc tgctcaagag cctggaggcc
     2341 cagggcttca agtccgtggt cgctgtgatc ggcctcccca acgacccgag cgtccgtatg
     2401 cacgaggccc tcggctacgc gccccgcggg atgctgaggg ccgcgggctt caagcacggc
     2461 aactggcacg acgtgggctt ctggcagctc gacttctccc tgcctgtccc accccgcccg
     2521 gtgctccccg tcaccgaaat ctgaacgcgt tcgagtatta tggcattggg aaaactgttt
     2581 ttcttgtacc atttgttgtg cttgtaattt actgtgtttt ttattcggtt ttcgctatcg
     2641 aactgtgaaa tggaaatgga tggagaagag ttaatgaatg atatggtcct tttgttcatt
     2701 ctcaaattaa tattatttgt tttttctctt atttgttgtg tgttgaattt gaaattataa
     2761 gagatatgca aacattttgt tttgagtaaa aatgtgtcaa atcgtggcct ctaatgaccg
     2821 aagttaatat gaggagtaaa acactgaagc ctgcaggcat gcaagctgat ccactagagg
     2881 ccatggcggc cgcactagat caacatggtg gagcacgaca ctctcgtcta ctccaagaat
     2941 atcaaagata cagtctcaga agaccagagg gctattgaga cttttcaaca aagggtaata
     3001 tcgggaaacc tcctcggatt ccattgccca gctatctgtc acttcatcga aaggacagta
     3061 gaaaaggaag atggcttcta caaatgccat cattgcgata aaggaaaggc tatcgttcaa
     3121 gatgcctcta ccgacagtgg tcccaaagat ggacccccac ccacgaggaa catcgtggaa
     3181 aaagaagacg ttccaaccac gtcttcaaag caagtggatt gatgtgatat ctccactgac
     3241 gtaagggatg acgcacaatc ccactatcct tcgcaagacc cttcctctat ataaggaagt
     3301 tcatttcatt tggagaggac actagttgga tcccccgggc tgcaggaatt caagcttacg
     3361 cgtgtcgacc ctgctttaat gagatatgcg agacgcctat gatcgcatga tatttgcttt
     3421 caattctgtt gtgcacgttg taaaaaacct gagcatgtgt agctcagatc cttaccgccg
     3481 gtttcggttc attctaatga atatatcacc cgttactatc gtatttttat gaataatatt
     3541 ctccgttcaa tttactgatt gtcctcgaca ggccttaagg gccagatctt gggcccggta
     3601 cccgatcaga ttgtcgtttc ccgccttcgg tttaaactat cagtgtttga caggatatat
     3661 tggcgggtaa acctaagaga aaagagcgtt tattagaata atcggatatt taaaagggcg
     3721 tgaaaaggtt tatccgttcg tccatttgta tgtgcatgcc aaccacaggg ttcccctcgg
     3781 gagtgcttgg cattccgtgc gataatgact tctgttcaac cacccaaacg tcggaaagcc
     3841 tgacgacgga gcagcattcc aaaaagatcc cttggctcgt ctgggtcggc tagaaggtcg
     3901 agtgggctgc tgtggcttga tccctcaacg cggtcgcgga cgtagcgcag cgccgaaaaa
     3961 tcctcgatcg caaatccgac gctgtcgaaa agcgtgatct gcttgtcgct ctttcggccg
     4021 acgtcctggc cagtcatcac gcgccaaagt tccgtcacag gatgatctgg cgcgagttgc
     4081 tggatctcgc cttcaatccg ggtctgtggc gggaactcca cgaaaatatc cgaacgcagc
     4141 aagatatcgc ggtgcatctc ggtcttgcct gggcagtcgc cgccgacgcc gttgatgtgg
     4201 acgccgaaaa ggatctaggt gaagatcctt tttgataatc tcatgaccaa aatcccttaa
     4261 cgtgagtttt cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga
     4321 gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg
     4381 gtggtttgtt tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc
     4441 agagcgcaga taccaaatac tgttcttcta gtgtagccgt agttaggcca ccacttcaag
     4501 aactctgtag caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc
     4561 agtggcgata agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg
     4621 cagcggtcgg gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac
     4681 accgaactga gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga
     4741 aaggcggaca ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt
     4801 ccagggggaa acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag
     4861 cgtcgatttt tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg
     4921 gcctttttac ggttcctggc cttttgctgg ccttttgctc acatgttctt tcctgcgtta
     4981 tcccctgatt ctgtggataa ccgattaccg cctttgagtg agctgatacc gctcgccgca
     5041 gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc ggaagagcgc ctgatgcggt
     5101 attttctcct tacgcatctg tgcggtattt cacaccgcat atggtgcact ctcagtacaa
     5161 tctgctctga tgccgcatag ttaagccagt atacactccg ctatcgctac gtgactgggt
     5221 catggctgcg ccccgacacc cgccaacacc cgctgacgcg ccctgacggg cttgtctgct
     5281 cccggcatcc gcttacagac aagctgtgac cgtctccggg agctgcatgt gtcagaggtt
     5341 ttcaccgtca tcaccgaaac gcgcgaggca ggggtacgtc gaggtcgatc caacccctcc
     5401 gctgctatag tgcagtcggc ttctgacgtt cagtgcagcc gtcttctgaa aacgacatgt
     5461 cgcacaagtc ctaagttacg cgacaggctg ccgccctgcc cttttcctgg cgttttcttg
     5521 tcgcgtgttt tagtcgcata aagtagaata cttgcgacta gaaccggaga cattacgcca
     5581 tgaacaagag cgccgccgct ggcctgctgg gctatgcccg cgtcagcacc gacgaccagg
     5641 acttgaccaa ccaacgggcc gaactgcacg cggccggctg caccaagctg ttttccgaga
     5701 agatcaccgg caccaggcgc gaccgcccgg agctggccag gatgcttgac cacctacgcc
     5761 ctggcgacgt tgtgacagtg accaggctag accgcctggc ccgcagcacc cgcgacctac
     5821 tggacattgc cgagcgcatc caggaggccg gcgcgggcct gcgtagcctg gcagagccgt
     5881 gggccgacac caccacgccg gccggccgca tggtgttgac cgtgttcgcc ggcattgccg
     5941 agttcgagcg ttccctaatc atcgaccgca cccggagcgg gcgcgaggcc gccaaggcgc
     6001 gaggcgtgaa gtttggcccc cgccctaccc tcaccccggc acagatcgcg cacgcccgcg
     6061 agctgatcga ccaggaaggc cgcaccgtga aagaggcggc tgcactgctt ggcgtgcatc
     6121 gctcgaccct gtaccgcgca cttgagcgca gcgaggaagt gacgcccacc gaggccaggc
     6181 ggcgcggtgc cttccgtgag gacgcattga ccgaggccga cgccctggcg gccgccgaga
     6241 atgaacgcca agaggaacaa gcatgaaacc gcaccaggac ggccaggacg aaccgttttt
     6301 cattaccgaa gagatcgagg cggagatgat cgcggccggg tacgtgttcg agccgcccgc
     6361 gcacgtctca accgtgcggc tgcatgaaat cctggccggt ttgtctgatg ccaagctcgc
     6421 ggcctggccg gcgagcttgg ccgctgaaga aaccgagcgc cgccgtctaa aaaggtgatg
     6481 tgtatttgag taaaacagct tgcgtcatgc ggtcgctgcg tatatgatgc gatgagtaaa
     6541 taaacaaata cgcaagggga acgcatgaag gttatcgctg tacttaacca gaaaggcggg
     6601 tcaggcaaga cgaccatcgc aacccatcta gcccgcgccc tgcaactcgc cggggccgat
     6661 gttctgttag tcgattccga tccccagggc agtgcccgcg attgggcggc cgtgcgggaa
     6721 gatcaaccgc taaccgttgt cggcatcgac cgcccgacga ttgaccgcga cgtgaaggcc
     6781 atcggccggc gcgacttcgt agtgatcgac ggagcgcccc aggcggcgga cttggctgtg
     6841 tccgcgatca aggcagccga cttcgtgctg attccggtgc agccaagccc ttacgacata
     6901 tgggccaccg ccgacctggt ggagctggtt aagcagcgca ttgaggtcac ggatggaagg
     6961 ctacaagcgg cctttgtcgt gtcgcgggcg atcaaaggca cgcgcatcgg cggtgaggtt
     7021 gccgaggcgc tggccgggta cgagctgccc attcttgagt cccgtatcac gcagcgcgtg
     7081 agctacccag gcactgccgc cgccggcaca accgttcttg aatcagaacc cgagggcgac
     7141 gctgcccgcg aggtccaggc gctggccgct gaaattaaat caaaactcat ttgagttaat
     7201 gaggtaaaga gaaaatgagc aaaagcacaa acacgctaag tgccggccgt ccgagcgcac
     7261 gcagcagcaa ggctgcaacg ttggccagcc tggcagacac gccagccatg aagcgggtca
     7321 actttcagtt gccggcggag gatcacacca agctgaagat gtacgcggta cgccaaggca
     7381 agaccattac cgagctgcta tctgaataca tcgcgcagct accagagtaa atgagcaaat
     7441 gaataaatga gtagatgaat tttagcggct aaaggaggcg gcatggaaaa tcaagaacaa
     7501 ccaggcaccg acgccgtgga atgccccatg tgtggaggaa cgggcggttg gccaggcgta
     7561 agcggctggg ttgtctgccg gccctgcaat ggcactggaa cccccaagcc cgaggaatcg
     7621 gcgtgagcgg tcgcaaacca tccggcccgg tacaaatcgg cgcggcgctg ggtgatgacc
     7681 tggtggagaa gttgaaggcg gcgcaggccg cccagcggca acgcatcgag gcagaagcac
     7741 gccccggtga atcgtggcaa gcggccgctg atcgaatccg caaagaatcc cggcaaccgc
     7801 cggcagccgg tgcgccgtcg attaggaagc cgcccaaggg cgacgagcaa ccagattttt
     7861 tcgttccgat gctctatgac gtgggcaccc gcgatagtcg cagcatcatg gacgtggccg
     7921 ttttccgtct gtcgaagcgt gaccgacgag ctggcgaggt gatccgctac gagcttccag
     7981 acgggcacgt agaggtttcc gcagggccgg ccggcatggc gagtgtgtgg gattacgacc
     8041 tggtactgat ggcggtttcc catctaaccg aatccatgaa ccgataccgg gaagggaagg
     8101 gagacaagcc cggccgcgtg ttccgtccac acgttgcgga cgtactcaag ttctgccggc
     8161 gagccgatgg cggaaagcag aaagacgacc tggtagaaac ctgcattcgg ttaaacacca
     8221 cgcacgttgc catgcagcgt acgaagaagg ccaagaacgg ccgcctggtg acggtatccg
     8281 agggtgaagc cttgattagc cgctacaaga tcgtaaagag cgaaaccggg cggccggagt
     8341 acatcgagat cgagctagct gattggatgt accgcgagat cacagaaggc aagaacccgg
     8401 acgtgctgac ggttcacccc gattactttt tgatcgatcc cggcatcggc cgttttctct
     8461 accgcctggc acgccgcgcc gcaggcaagg cagaagccag atggttgttc aagacgatct
     8521 acgaacgcag tggcagcgcc ggagagttca agaagttctg tttcaccgtg cgcaagctga
     8581 tcgggtcaaa tgacctgccg gagtacgatt tgaaggagga ggcggggcag gctggcccga
     8641 tcctagtcat gcgctaccgc aacctgatcg agggcgaagc atccgccggt tcctaatgta
     8701 cggagcagat gctagggcaa attgccctag caggggaaaa aggtcgaaaa ggtctctttc
     8761 ctgtggatag cacgtacatt gggaacccaa agccgtacat tgggaaccgg aacccgtaca
     8821 ttgggaaccc aaagccgtac attgggaacc ggtcacacat gtaagtgact gatataaaag
     8881 agaaaaaagg cgatttttcc gcctaaaact ctttaaaact tattaaaact cttaaaaccc
     8941 gcctggcctg tgcataactg tctggccagc gcacagccga agagctgcaa aaagcgccta
     9001 cccttcggtc gctgcgctcc ctacgccccg ccgcttcgcg tcggcctatc gcggccgctg
     9061 gccgctcaaa aatggctggc ctacggccag gcaatctacc agggcgcgga caagccgcgc
     9121 cgtcgccact cgaccgccgg cgcccacatc aaggcaccgg tgggtatgcc tgacgatgcg
     9181 tggagaccga aaccttgcgc tcgttcgcca gccaggacag aaatgcctcg acttcgctgc
     9241 tgcccaaggt tgccgggtga cgcacaccgt ggaaacggat gaaggcacga acccagtgga
     9301 cataagcctg ttcggttcgt aagctgtaat gcaagtagcg tatgcgctca cgcaactggt
     9361 ccagaacctt gaccgaacgc agcggtggta acggcgcagt ggcggttttc atggcttgtt
     9421 atgactgttt ttttggggta cagtctatgc ctcgggcatc caagcagcaa gcgcgttacg
     9481 ccgtgggtcg atgtttgatg ttatggagca gcaacgatgt tacgcagcag ggcagtcgcc
     9541 ctaaaacaaa gttaaacatc atgagggaag cggtgatcgc cgaagtatcg actcaactat
     9601 cagaggtagt tggcgtcatc gagcgccatc tcgaaccgac gttgctggcc gtacatttgt
     9661 acggctccgc agtggatggc ggcctgaagc cacacagtga tattgatttg ctggttacgg
     9721 tgaccgtaag gcttgatgaa acaacgcggc gagctttgat caacgacctt ttggaaactt
     9781 cggcttcccc tggagagagc gagattctcc gcgctgtaga agtcaccatt gttgtgcacg
     9841 acgacatcat tccgtggcgt tatccagcta agcgcgaact gcaatttgga gaatggcagc
     9901 gcaatgacat tcttgcaggt atcttcgagc cagccacgat cgacattgat ctggctatct
     9961 tgctgacaaa agcaagagaa catagcgttg ccttggtagg tccagcggcg gaggaactct
    10021 ttgatccggt tcctgaacag gatctatttg aggcgctaaa tgaaacctta acgctatgga
    10081 actcgccgcc cgactgggct ggcgatgagc gaaatgtagt gcttacgttg tcccgcattt
    10141 ggtacagcgc agtaaccggc aaaatcgcgc cgaaggatgt cgctgccgac tgggcaatgg
    10201 agcgcctgcc ggcccagtat cagcccgtca tacttgaagc tagacaggct tatcttggac
    10261 aagaagaaga tcgcttggcc tcgcgcgcag atcagttgga agaatttgtc cactacgtga
    10321 aaggcgagat caccaaggta gtcggcaaat aatgtctaac aattcgttca agccgacgcc
    10381 gcttcgcggc gcggcttaac tcaagcgtta gatgcactaa gcacataatt gctcacagcc
    10441 aaactatcag gtcaagtctg cttttattat ttttaagcgt gcataataag ccctacacaa
    10501 attgggagat atatcatgaa aggctggctt tttcttgtta tcgcaatagt tggcgaagta
    10561 atcgcaacat agcttgcttg gtcgttccgc gtgaacgtcg gctcgattgt acctgcgttc
    10621 aaatactttg cgatcgtgtt gcgcgcctgc ccggtgcgtc ggctgatctc acggatcgac
    10681 tgcttctctc gcaacgccat ccgacggatg atgtttaaaa gtcccatgtg gatcactccg
    10741 ttgccccgtc gctcaccgtg ttggggggaa ggtgcacatg gctcagttct caatggaaat
    10801 tatctgccta accggctcag ttctgcgtag aaaccaacat gcaagctcca ccgggtgcaa
    10861 agcggcagcg gc
//
