LOCUS       B434p7oU10-LH          10244 bp    DNA     circular SYN 22-JAN-2016
DEFINITION  Ligation of U10-Bsa-Xho* into B414p7o-LH*
ACCESSION   B434p7oU10-LH
KEYWORDS    .
SOURCE      Unknown.
  ORGANISM  Unknown
            Unclassified.
REFERENCE   1  (bases 1 to 10244)
  AUTHORS   Self
  JOURNAL   Unpublished.
COMMENT     SECID/File created by Clone Manager, Scientific & Educational Software
COMMENT     SECNOTES|Vector molecule:  B414p7o-LH*
               Fragment ends: XhoI and XbaI
               Fragment size: 8761
            Insert molecule:  U10-Bsa-Xho*
               Fragment ends: BsaI and XhoI
               Fragment size: 1483
            Primer for gene synthesis
            BsaU10	ccGGTCTCtctagaTTTCCTTACATTCTGAGCC
            XhoU10	CCCGTCctcgaGTACCTGTTAATCAGAAAAACTC
            Sequencing primers	
            LHX1	CTTTATTTCGACGTGTCTAC
            baroR	GGTCTCGATGTAGTGGTTCA
FEATURES             Location/Qualifiers
     misc_feature    1..24
                     /gene="LB"
                     /product="Left Border"
                     /SECDrawAs="Region"
                     /SECStyleId=1
     misc_feature    309..1784
                     /gene="UBQ10"
                     /product="PUbq10At"
                     /SECDrawAs="Region"
                     /SECStyleId=1
     misc_feature    1413..1476
                     /product="Ubi cds 5"
                     /SECDrawAs="Region"
                     /SECStyleId=1
     misc_feature    1479..1782
                     /gene="int"
                     /SECDrawAs="Region"
                     /SECStyleId=1
     CDS             1804..2037
                     /gene="bar"
                     /SECDrawAs="Gene"
                     /SECStyleId=1
     misc_feature    2038..2226
                     /gene="int"
                     /product="STLS1"
                     /SECDrawAs="Gene"
                     /SECStyleId=1
                     /SECName="int"
                     /SECDescr="STLS1"
     CDS             2227..2542
                     /gene="bar"
                     /SECDrawAs="Gene"
                     /SECStyleId=1
     misc_feature    2555..2845
                     /gene="TE9"
                     /product="Terminator Rbcs-E9"
                     /SECDrawAs="Region"
                     /SECStyleId=1
     misc_feature    3021..3044
                     /gene="RB"
                     /product="Right Border"
                     /SECDrawAs="Region"
                     /SECStyleId=1
     misc_feature    3579..4276
                     /gene="ColE1"
                     /product="ColE1 ORI"
                     /SECDrawAs="Region"
                     /SECStyleId=1
     misc_feature    4313..4572
                     /gene="bom"
                     /SECDrawAs="Region"
                     /SECStyleId=1
     misc_feature    4825..8530
                     /gene="pVS1"
                     /product="ori for agrobacteria"
                     /SECDrawAs="Region"
                     /SECStyleId=1
     CDS             8714..9724
                     /gene="Sm/Sp"
                     /product="Strep/Spec resistence"
                     /SECDrawAs="Gene"
                     /SECStyleId=1
ORIGIN      
        1 ggcaggatat attcaattgt aaatggcttc atgtccggga aatctacatg gatcagcaat
       61 gagtatgatg gtcaatatgg agaaaaagaa agagtaatta ccaatttttt ttcaattcaa
      121 aaatgtagat gtccgcagcg ttattataaa atgaaagtac attttgataa aacgacaaat
      181 tacgatccgt cgtatttata ggcgaaagca ataaacaaat tattctaatt cggaaatctt
      241 tatttcgacg tgtctacatt cacgtccaaa tgggggctta gatgagaaac ttcacgatcg
      301 gctctagatt tccttacatt ctgagcctct ttccttctaa tccactcatc tgcatcttct
      361 tgtgtcctta ctaatacctc attggttcca aattccctcc ctttaagcac cagctcgttt
      421 ctgttcttcc acagcctccc aagtatccaa gggactaaag cctccacatt cttcagatca
      481 ggatattctt gtttaagatg ttgaactcta tggaggtttg tatgaactga tgatctagga
      541 ccggataagt tcccttcttc atagcgaact tattcaaaga atgttttgtg tatcattctt
      601 gttacattgt tattaatgaa aaaatattat tggtcattgg actgaacacg agtgttaaat
      661 atggaccagg ccccaaataa gatccattga tatatgaatt aaataacaag aataaatcga
      721 gtcaccaaac cacttgcctt ttttaacgag acttgttcac caacttgata caaaagtcat
      781 tatcctatgc aaatcaataa tcatacaaaa atatccaata acactaaaaa attaaaagaa
      841 atggataatt tcacaatatg ttatacgata aagaagttac ttttccaaga aattcactga
      901 ttttataagc ccacttgcat tagataaatg gcaaaaaaaa acaaaaagga aaagaaataa
      961 agcacgaaga attctagaaa atacgaaata cgcttcaatg cagtgggacc cacggttcaa
     1021 ttattgccaa ttttcagctc caccgtatat ttaaaaaata aaacgataat gctaaaaaaa
     1081 tataaatcgt aacgatcgtt aaatctcaac ggctggatct tatgacgacc gttagaaatt
     1141 gtggttgtcg acgagtcagt aataaacggc gtcaaagtgg ttgcagccgg cacacacgag
     1201 tcgtgtttat caactcaaag cacaaatact tttcctcaac ctaaaaataa ggcaattagc
     1261 caaaaacaac tttgcgtgta aacaacgctc aatacacgtg tcattttatt attagctatt
     1321 gcttcaccgc cttagctttc tcgtgaccta gtcgtcctcg tcttttcttc ttcttcttct
     1381 ataaaacaat acccaaagag ctcttcttct tcacaattca gatttcaatt tctcaaaatc
     1441 ttaaaaactt tctctcaatt ctctctaccg tgatcaaggt aaatttctgt gttccttatt
     1501 ctctcaaaat cttcgatttt gttttcgttc gatcccaatt tcgtatatgt tctttggttt
     1561 agattctgtt aatcttagat cgaagacgat tttctgggtt tgatcgttag atatcatctt
     1621 aattctcgat tagggtttca tagatatcat ccgatttgtt caaataattt gagttttgtc
     1681 gaataattac tcttcgattt gtgatttcta tctagatctg gtgttagttt ctagtttgtg
     1741 cgatcgaatt tgtcgattaa tctgagtttt tctgattaac aggtactcga gtcctccata
     1801 actatgggcc ccgagcgcag gcccgccgac atccgccgtg ccaccgaggc cgacatgcca
     1861 gccgtctgca ccatcgtgaa ccactacatc gagacctcca cggtcaactt ccgcacagag
     1921 ccgcaggagc ctcaggagtg gaccgacgac ctcgtgaggc tgcgcgagcg ctacccctgg
     1981 ctcgtcgctg aggtggatgg cgaggtcgcc ggcatcgcct acgcgggccc ctggaaggta
     2041 agtttctgct tctacctttg atatatatat aataattatc attaattagt agtaatataa
     2101 tatttcaaat atttttttca aaataaaaga atgtagtata tagcaattgc ttttctgtag
     2161 tttataagtg tgtatatttt aatttataac ttttctaata tatgaccaaa atttgttgat
     2221 gtgcaggcac gcaacgccta cgactggacg gcggagagca ccgtctacgt gtccccacgc
     2281 caccagcgca ccggcctggg ctccacgctc tacacccacc tgctcaagag cctggaggcc
     2341 cagggcttca agtccgtggt cgctgtgatc ggcctcccca acgacccgag cgtccgtatg
     2401 cacgaggccc tcggctacgc gccccgcggg atgctgaggg ccgcgggctt caagcacggc
     2461 aactggcacg acgtgggctt ctggcagctc gacttctccc tgcctgtccc accccgcccg
     2521 gtgctccccg tcaccgaaat ctgaacgcgt tcgagtatta tggcattggg aaaactgttt
     2581 ttcttgtacc atttgttgtg cttgtaattt actgtgtttt ttattcggtt ttcgctatcg
     2641 aactgtgaaa tggaaatgga tggagaagag ttaatgaatg atatggtcct tttgttcatt
     2701 ctcaaattaa tattatttgt tttttctctt atttgttgtg tgttgaattt gaaattataa
     2761 gagatatgca aacattttgt tttgagtaaa aatgtgtcaa atcgtggcct ctaatgaccg
     2821 aagttaatat gaggagtaaa acactgaagc ctgcaggcat gcaagctgat ccactagagg
     2881 ccatggcggc cgcactagtg gatcccccgg gatgcatgaa ttcaagctta tcgatgtcga
     2941 caggccttaa gggccagatc ttgggcccgg tacccgatca gattgtcgtt tcccgccttc
     3001 ggtttaaact atcagtgttt gacaggatat attggcgggt aaacctaaga gaaaagagcg
     3061 tttattagaa taatcggata tttaaaaggg cgtgaaaagg tttatccgtt cgtccatttg
     3121 tatgtgcatg ccaaccacag ggttcccctc gggagtgctt ggcattccgt gcgataatga
     3181 cttctgttca accacccaaa cgtcggaaag cctgacgacg gagcagcatt ccaaaaagat
     3241 cccttggctc gtctgggtcg gctagaaggt cgagtgggct gctgtggctt gatccctcaa
     3301 cgcggtcgcg gacgtagcgc agcgccgaaa aatcctcgat cgcaaatccg acgctgtcga
     3361 aaagcgtgat ctgcttgtcg ctctttcggc cgacgtcctg gccagtcatc acgcgccaaa
     3421 gttccgtcac aggatgatct ggcgcgagtt gctggatctc gccttcaatc cgggtctgtg
     3481 gcgggaactc cacgaaaata tccgaacgca gcaagatatc gcggtgcatc tcggtcttgc
     3541 ctgggcagtc gccgccgacg ccgttgatgt ggacgccgaa aaggatctag gtgaagatcc
     3601 tttttgataa tctcatgacc aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag
     3661 accccgtaga aaagatcaaa ggatcttctt gagatccttt ttttctgcgc gtaatctgct
     3721 gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg tttgccggat caagagctac
     3781 caactctttt tccgaaggta actggcttca gcagagcgca gataccaaat actgttcttc
     3841 tagtgtagcc gtagttaggc caccacttca agaactctgt agcaccgcct acatacctcg
     3901 ctctgctaat cctgttacca gtggctgctg ccagtggcga taagtcgtgt cttaccgggt
     3961 tggactcaag acgatagtta ccggataagg cgcagcggtc gggctgaacg gggggttcgt
     4021 gcacacagcc cagcttggag cgaacgacct acaccgaact gagataccta cagcgtgagc
     4081 tatgagaaag cgccacgctt cccgaaggga gaaaggcgga caggtatccg gtaagcggca
     4141 gggtcggaac aggagagcgc acgagggagc ttccaggggg aaacgcctgg tatctttata
     4201 gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg
     4261 ggcggagcct atggaaaaac gccagcaacg cggccttttt acggttcctg gccttttgct
     4321 ggccttttgc tcacatgttc tttcctgcgt tatcccctga ttctgtggat aaccgattac
     4381 cgcctttgag tgagctgata ccgctcgccg cagccgaacg accgagcgca gcgagtcagt
     4441 gagcgaggaa gcggaagagc gcctgatgcg gtattttctc cttacgcatc tgtgcggtat
     4501 ttcacaccgc atatggtgca ctctcagtac aatctgctct gatgccgcat agttaagcca
     4561 gtatacactc cgctatcgct acgtgactgg gtcatggctg cgccccgaca cccgccaaca
     4621 cccgctgacg cgccctgacg ggcttgtctg ctcccggcat ccgcttacag acaagctgtg
     4681 accgtctccg ggagctgcat gtgtcagagg ttttcaccgt catcaccgaa acgcgcgagg
     4741 caggggtacg tcgaggtcga tccaacccct ccgctgctat agtgcagtcg gcttctgacg
     4801 ttcagtgcag ccgtcttctg aaaacgacat gtcgcacaag tcctaagtta cgcgacaggc
     4861 tgccgccctg cccttttcct ggcgttttct tgtcgcgtgt tttagtcgca taaagtagaa
     4921 tacttgcgac tagaaccgga gacattacgc catgaacaag agcgccgccg ctggcctgct
     4981 gggctatgcc cgcgtcagca ccgacgacca ggacttgacc aaccaacggg ccgaactgca
     5041 cgcggccggc tgcaccaagc tgttttccga gaagatcacc ggcaccaggc gcgaccgccc
     5101 ggagctggcc aggatgcttg accacctacg ccctggcgac gttgtgacag tgaccaggct
     5161 agaccgcctg gcccgcagca cccgcgacct actggacatt gccgagcgca tccaggaggc
     5221 cggcgcgggc ctgcgtagcc tggcagagcc gtgggccgac accaccacgc cggccggccg
     5281 catggtgttg accgtgttcg ccggcattgc cgagttcgag cgttccctaa tcatcgaccg
     5341 cacccggagc gggcgcgagg ccgccaaggc gcgaggcgtg aagtttggcc cccgccctac
     5401 cctcaccccg gcacagatcg cgcacgcccg cgagctgatc gaccaggaag gccgcaccgt
     5461 gaaagaggcg gctgcactgc ttggcgtgca tcgctcgacc ctgtaccgcg cacttgagcg
     5521 cagcgaggaa gtgacgccca ccgaggccag gcggcgcggt gccttccgtg aggacgcatt
     5581 gaccgaggcc gacgccctgg cggccgccga gaatgaacgc caagaggaac aagcatgaaa
     5641 ccgcaccagg acggccagga cgaaccgttt ttcattaccg aagagatcga ggcggagatg
     5701 atcgcggccg ggtacgtgtt cgagccgccc gcgcacgtct caaccgtgcg gctgcatgaa
     5761 atcctggccg gtttgtctga tgccaagctc gcggcctggc cggcgagctt ggccgctgaa
     5821 gaaaccgagc gccgccgtct aaaaaggtga tgtgtatttg agtaaaacag cttgcgtcat
     5881 gcggtcgctg cgtatatgat gcgatgagta aataaacaaa tacgcaaggg gaacgcatga
     5941 aggttatcgc tgtacttaac cagaaaggcg ggtcaggcaa gacgaccatc gcaacccatc
     6001 tagcccgcgc cctgcaactc gccggggccg atgttctgtt agtcgattcc gatccccagg
     6061 gcagtgcccg cgattgggcg gccgtgcggg aagatcaacc gctaaccgtt gtcggcatcg
     6121 accgcccgac gattgaccgc gacgtgaagg ccatcggccg gcgcgacttc gtagtgatcg
     6181 acggagcgcc ccaggcggcg gacttggctg tgtccgcgat caaggcagcc gacttcgtgc
     6241 tgattccggt gcagccaagc ccttacgaca tatgggccac cgccgacctg gtggagctgg
     6301 ttaagcagcg cattgaggtc acggatggaa ggctacaagc ggcctttgtc gtgtcgcggg
     6361 cgatcaaagg cacgcgcatc ggcggtgagg ttgccgaggc gctggccggg tacgagctgc
     6421 ccattcttga gtcccgtatc acgcagcgcg tgagctaccc aggcactgcc gccgccggca
     6481 caaccgttct tgaatcagaa cccgagggcg acgctgcccg cgaggtccag gcgctggccg
     6541 ctgaaattaa atcaaaactc atttgagtta atgaggtaaa gagaaaatga gcaaaagcac
     6601 aaacacgcta agtgccggcc gtccgagcgc acgcagcagc aaggctgcaa cgttggccag
     6661 cctggcagac acgccagcca tgaagcgggt caactttcag ttgccggcgg aggatcacac
     6721 caagctgaag atgtacgcgg tacgccaagg caagaccatt accgagctgc tatctgaata
     6781 catcgcgcag ctaccagagt aaatgagcaa atgaataaat gagtagatga attttagcgg
     6841 ctaaaggagg cggcatggaa aatcaagaac aaccaggcac cgacgccgtg gaatgcccca
     6901 tgtgtggagg aacgggcggt tggccaggcg taagcggctg ggttgtctgc cggccctgca
     6961 atggcactgg aacccccaag cccgaggaat cggcgtgagc ggtcgcaaac catccggccc
     7021 ggtacaaatc ggcgcggcgc tgggtgatga cctggtggag aagttgaagg cggcgcaggc
     7081 cgcccagcgg caacgcatcg aggcagaagc acgccccggt gaatcgtggc aagcggccgc
     7141 tgatcgaatc cgcaaagaat cccggcaacc gccggcagcc ggtgcgccgt cgattaggaa
     7201 gccgcccaag ggcgacgagc aaccagattt tttcgttccg atgctctatg acgtgggcac
     7261 ccgcgatagt cgcagcatca tggacgtggc cgttttccgt ctgtcgaagc gtgaccgacg
     7321 agctggcgag gtgatccgct acgagcttcc agacgggcac gtagaggttt ccgcagggcc
     7381 ggccggcatg gcgagtgtgt gggattacga cctggtactg atggcggttt cccatctaac
     7441 cgaatccatg aaccgatacc gggaagggaa gggagacaag cccggccgcg tgttccgtcc
     7501 acacgttgcg gacgtactca agttctgccg gcgagccgat ggcggaaagc agaaagacga
     7561 cctggtagaa acctgcattc ggttaaacac cacgcacgtt gccatgcagc gtacgaagaa
     7621 ggccaagaac ggccgcctgg tgacggtatc cgagggtgaa gccttgatta gccgctacaa
     7681 gatcgtaaag agcgaaaccg ggcggccgga gtacatcgag atcgagctag ctgattggat
     7741 gtaccgcgag atcacagaag gcaagaaccc ggacgtgctg acggttcacc ccgattactt
     7801 tttgatcgat cccggcatcg gccgttttct ctaccgcctg gcacgccgcg ccgcaggcaa
     7861 ggcagaagcc agatggttgt tcaagacgat ctacgaacgc agtggcagcg ccggagagtt
     7921 caagaagttc tgtttcaccg tgcgcaagct gatcgggtca aatgacctgc cggagtacga
     7981 tttgaaggag gaggcggggc aggctggccc gatcctagtc atgcgctacc gcaacctgat
     8041 cgagggcgaa gcatccgccg gttcctaatg tacggagcag atgctagggc aaattgccct
     8101 agcaggggaa aaaggtcgaa aaggtctctt tcctgtggat agcacgtaca ttgggaaccc
     8161 aaagccgtac attgggaacc ggaacccgta cattgggaac ccaaagccgt acattgggaa
     8221 ccggtcacac atgtaagtga ctgatataaa agagaaaaaa ggcgattttt ccgcctaaaa
     8281 ctctttaaaa cttattaaaa ctcttaaaac ccgcctggcc tgtgcataac tgtctggcca
     8341 gcgcacagcc gaagagctgc aaaaagcgcc tacccttcgg tcgctgcgct ccctacgccc
     8401 cgccgcttcg cgtcggccta tcgcggccgc tggccgctca aaaatggctg gcctacggcc
     8461 aggcaatcta ccagggcgcg gacaagccgc gccgtcgcca ctcgaccgcc ggcgcccaca
     8521 tcaaggcacc ggtgggtatg cctgacgatg cgtggagacc gaaaccttgc gctcgttcgc
     8581 cagccaggac agaaatgcct cgacttcgct gctgcccaag gttgccgggt gacgcacacc
     8641 gtggaaacgg atgaaggcac gaacccagtg gacataagcc tgttcggttc gtaagctgta
     8701 atgcaagtag cgtatgcgct cacgcaactg gtccagaacc ttgaccgaac gcagcggtgg
     8761 taacggcgca gtggcggttt tcatggcttg ttatgactgt ttttttgggg tacagtctat
     8821 gcctcgggca tccaagcagc aagcgcgtta cgccgtgggt cgatgtttga tgttatggag
     8881 cagcaacgat gttacgcagc agggcagtcg ccctaaaaca aagttaaaca tcatgaggga
     8941 agcggtgatc gccgaagtat cgactcaact atcagaggta gttggcgtca tcgagcgcca
     9001 tctcgaaccg acgttgctgg ccgtacattt gtacggctcc gcagtggatg gcggcctgaa
     9061 gccacacagt gatattgatt tgctggttac ggtgaccgta aggcttgatg aaacaacgcg
     9121 gcgagctttg atcaacgacc ttttggaaac ttcggcttcc cctggagaga gcgagattct
     9181 ccgcgctgta gaagtcacca ttgttgtgca cgacgacatc attccgtggc gttatccagc
     9241 taagcgcgaa ctgcaatttg gagaatggca gcgcaatgac attcttgcag gtatcttcga
     9301 gccagccacg atcgacattg atctggctat cttgctgaca aaagcaagag aacatagcgt
     9361 tgccttggta ggtccagcgg cggaggaact ctttgatccg gttcctgaac aggatctatt
     9421 tgaggcgcta aatgaaacct taacgctatg gaactcgccg cccgactggg ctggcgatga
     9481 gcgaaatgta gtgcttacgt tgtcccgcat ttggtacagc gcagtaaccg gcaaaatcgc
     9541 gccgaaggat gtcgctgccg actgggcaat ggagcgcctg ccggcccagt atcagcccgt
     9601 catacttgaa gctagacagg cttatcttgg acaagaagaa gatcgcttgg cctcgcgcgc
     9661 agatcagttg gaagaatttg tccactacgt gaaaggcgag atcaccaagg tagtcggcaa
     9721 ataatgtcta acaattcgtt caagccgacg ccgcttcgcg gcgcggctta actcaagcgt
     9781 tagatgcact aagcacataa ttgctcacag ccaaactatc aggtcaagtc tgcttttatt
     9841 atttttaagc gtgcataata agccctacac aaattgggag atatatcatg aaaggctggc
     9901 tttttcttgt tatcgcaata gttggcgaag taatcgcaac atagcttgct tggtcgttcc
     9961 gcgtgaacgt cggctcgatt gtacctgcgt tcaaatactt tgcgatcgtg ttgcgcgcct
    10021 gcccggtgcg tcggctgatc tcacggatcg actgcttctc tcgcaacgcc atccgacgga
    10081 tgatgtttaa aagtcccatg tggatcactc cgttgccccg tcgctcaccg tgttgggggg
    10141 aaggtgcaca tggctcagtt ctcaatggaa attatctgcc taaccggctc agttctgcgt
    10201 agaaaccaac atgcaagctc caccgggtgc aaagcggcag cggc
//
