LOCUS       B435p9oU10-LH          10496 bp    DNA     circular SYN 22-JAN-2016
DEFINITION  Ligation of U10-Bsa-Xho* into B415p9o-LH*
ACCESSION   B435p9oU10-LH
KEYWORDS    .
SOURCE      Unknown.
  ORGANISM  Unknown
            Unclassified.
REFERENCE   1  (bases 1 to 10496)
  AUTHORS   Self
  JOURNAL   Unpublished.
COMMENT     SECID/File created by Clone Manager, Scientific & Educational Software
COMMENT     SECNOTES|Vector molecule:  B415p9o-LH*
               Fragment ends: XhoI and XbaI
               Fragment size: 9013
            Insert molecule:  U10-Bsa-Xho*
               Fragment ends: BsaI and XhoI
               Fragment size: 1483
            Primer for gene synthesis
            BsaU10	ccGGTCTCtctagaTTTCCTTACATTCTGAGCC
            XhoU10	CCCGTCctcgaGTACCTGTTAATCAGAAAAACTC
            Sequencing primers	
            LHX1	CTTTATTTCGACGTGTCTAC
            nptoR	CACTGCACCCAATGGTCTGTT
            
FEATURES             Location/Qualifiers
     misc_feature    1..24
                     /gene="LB"
                     /product="Left Border"
                     /SECDrawAs="Region"
                     /SECStyleId=1
     misc_feature    309..1784
                     /gene="UBQ10"
                     /product="PUbq10At"
                     /SECDrawAs="Region"
                     /SECStyleId=1
     misc_feature    1413..1476
                     /product="Ubi cds 5"
                     /SECDrawAs="Region"
                     /SECStyleId=1
     misc_feature    1479..1782
                     /gene="int"
                     /SECDrawAs="Region"
                     /SECStyleId=1
     CDS             1804..2102
                     /gene="nptIIo"
                     /product="NptII optimiert"
                     /SECDrawAs="Gene"
                     /SECStyleId=1
     misc_signal     2099..2102
                     /label=splice
                     /SECDrawAs="Label"
     CDS             2103..2289
                     /gene="int"
                     /SECDrawAs="Gene"
                     /SECStyleId=1
     misc_signal     2288..2291
                     /label=splice
                     /SECDrawAs="Label"
     CDS             2290..2793
                     /gene="nptIIo"
                     /product="NptII optimiert"
                     /SECDrawAs="Gene"
                     /SECStyleId=1
     misc_feature    2807..3097
                     /gene="TE9"
                     /product="Terminator Rbcs-E9"
                     /SECDrawAs="Region"
                     /SECStyleId=1
     misc_feature    3273..3296
                     /gene="RB"
                     /product="Right Border"
                     /SECDrawAs="Region"
                     /SECStyleId=1
     misc_feature    3831..4528
                     /gene="ColE1"
                     /product="ColE1 ORI"
                     /SECDrawAs="Region"
                     /SECStyleId=1
     misc_feature    4565..4824
                     /gene="bom"
                     /SECDrawAs="Region"
                     /SECStyleId=1
     misc_feature    5077..8782
                     /gene="pVS1"
                     /product="ori for agrobacteria"
                     /SECDrawAs="Region"
                     /SECStyleId=1
     CDS             8966..9976
                     /gene="Sm/Sp"
                     /product="Strep/Spec resistence"
                     /SECDrawAs="Gene"
                     /SECStyleId=1
ORIGIN      
        1 ggcaggatat attcaattgt aaatggcttc atgtccggga aatctacatg gatcagcaat
       61 gagtatgatg gtcaatatgg agaaaaagaa agagtaatta ccaatttttt ttcaattcaa
      121 aaatgtagat gtccgcagcg ttattataaa atgaaagtac attttgataa aacgacaaat
      181 tacgatccgt cgtatttata ggcgaaagca ataaacaaat tattctaatt cggaaatctt
      241 tatttcgacg tgtctacatt cacgtccaaa tgggggctta gatgagaaac ttcacgatcg
      301 gctctagatt tccttacatt ctgagcctct ttccttctaa tccactcatc tgcatcttct
      361 tgtgtcctta ctaatacctc attggttcca aattccctcc ctttaagcac cagctcgttt
      421 ctgttcttcc acagcctccc aagtatccaa gggactaaag cctccacatt cttcagatca
      481 ggatattctt gtttaagatg ttgaactcta tggaggtttg tatgaactga tgatctagga
      541 ccggataagt tcccttcttc atagcgaact tattcaaaga atgttttgtg tatcattctt
      601 gttacattgt tattaatgaa aaaatattat tggtcattgg actgaacacg agtgttaaat
      661 atggaccagg ccccaaataa gatccattga tatatgaatt aaataacaag aataaatcga
      721 gtcaccaaac cacttgcctt ttttaacgag acttgttcac caacttgata caaaagtcat
      781 tatcctatgc aaatcaataa tcatacaaaa atatccaata acactaaaaa attaaaagaa
      841 atggataatt tcacaatatg ttatacgata aagaagttac ttttccaaga aattcactga
      901 ttttataagc ccacttgcat tagataaatg gcaaaaaaaa acaaaaagga aaagaaataa
      961 agcacgaaga attctagaaa atacgaaata cgcttcaatg cagtgggacc cacggttcaa
     1021 ttattgccaa ttttcagctc caccgtatat ttaaaaaata aaacgataat gctaaaaaaa
     1081 tataaatcgt aacgatcgtt aaatctcaac ggctggatct tatgacgacc gttagaaatt
     1141 gtggttgtcg acgagtcagt aataaacggc gtcaaagtgg ttgcagccgg cacacacgag
     1201 tcgtgtttat caactcaaag cacaaatact tttcctcaac ctaaaaataa ggcaattagc
     1261 caaaaacaac tttgcgtgta aacaacgctc aatacacgtg tcattttatt attagctatt
     1321 gcttcaccgc cttagctttc tcgtgaccta gtcgtcctcg tcttttcttc ttcttcttct
     1381 ataaaacaat acccaaagag ctcttcttct tcacaattca gatttcaatt tctcaaaatc
     1441 ttaaaaactt tctctcaatt ctctctaccg tgatcaaggt aaatttctgt gttccttatt
     1501 ctctcaaaat cttcgatttt gttttcgttc gatcccaatt tcgtatatgt tctttggttt
     1561 agattctgtt aatcttagat cgaagacgat tttctgggtt tgatcgttag atatcatctt
     1621 aattctcgat tagggtttca tagatatcat ccgatttgtt caaataattt gagttttgtc
     1681 gaataattac tcttcgattt gtgatttcta tctagatctg gtgttagttt ctagtttgtg
     1741 cgatcgaatt tgtcgattaa tctgagtttt tctgattaac aggtactcga gtcctccata
     1801 actatgggag catggattga gcaggatggc ctccatgctg gtagccccgc tgcttgggtg
     1861 gagcgcctct ttggctacga ctgggcccaa cagaccattg ggtgcagtga tgccgctgtc
     1921 ttccgtttga gcgcccaggg caggccagtt ctcttcgtga agactgacct ctccggtgcc
     1981 ctcaacgagt tgcaggacga agctgcccgc ctctcatggc ttgccaccac cggagtccct
     2041 tgcgctgccg tgttggacgt tgtcacagag gccggaagag actggctgct cttgggcgag
     2101 gtaagtttct gcttctacct ttgatatata tataataatt atcattaatt agtagtaata
     2161 taatatttca aatatttttt tcaaaataaa agaatgtagt atatagcaat tgcttttctg
     2221 tagtttataa gtgtgtatat tttaatttat aacttttcta atatatgacc aaaatttgtt
     2281 gatgtgcagg tgccaggcca agacctcctg agctcccacc tggccccagc cgagaaggtc
     2341 tccatcatgg cagacgccat gcgtcgcctc cacaccctgg acccagccac ctgccccttc
     2401 gaccaccagg ccaagcacag gatcgaacgt gcacgcacta ggatggaggc cggccttgtg
     2461 gaccaggatg accttgacga ggagcaccag ggccttgctc ccgccgagct gttcgcccgc
     2521 ctcaaggcta gaatgcctga cggagaggac ttggtggtca cccacgggga tgcctgcctc
     2581 cccaacatca tggttgagaa cggacgcttc tctggcttca tcgactgcgg tcgtctgggc
     2641 gtggccgacc gctaccaaga catcgccctg gccaccaggg acatcgctga ggaacttgga
     2701 ggggagtggg ccgatcgttt cttggtcctc tacggcattg ctgcccccga ctcccagagg
     2761 atcgccttct accgcctgct tgacgagttc ttctgaacgc gttcgagtat tatggcattg
     2821 ggaaaactgt ttttcttgta ccatttgttg tgcttgtaat ttactgtgtt ttttattcgg
     2881 ttttcgctat cgaactgtga aatggaaatg gatggagaag agttaatgaa tgatatggtc
     2941 cttttgttca ttctcaaatt aatattattt gttttttctc ttatttgttg tgtgttgaat
     3001 ttgaaattat aagagatatg caaacatttt gttttgagta aaaatgtgtc aaatcgtggc
     3061 ctctaatgac cgaagttaat atgaggagta aaacactgaa gcctgcaggc atgcaagctg
     3121 atccactaga ggccatggcg gccgcactag tggatccccc gggatgcatg aattcaagct
     3181 tatcgatgtc gacaggcctt aagggccaga tcttgggccc ggtacccgat cagattgtcg
     3241 tttcccgcct tcggtttaaa ctatcagtgt ttgacaggat atattggcgg gtaaacctaa
     3301 gagaaaagag cgtttattag aataatcgga tatttaaaag ggcgtgaaaa ggtttatccg
     3361 ttcgtccatt tgtatgtgca tgccaaccac agggttcccc tcgggagtgc ttggcattcc
     3421 gtgcgataat gacttctgtt caaccaccca aacgtcggaa agcctgacga cggagcagca
     3481 ttccaaaaag atcccttggc tcgtctgggt cggctagaag gtcgagtggg ctgctgtggc
     3541 ttgatccctc aacgcggtcg cggacgtagc gcagcgccga aaaatcctcg atcgcaaatc
     3601 cgacgctgtc gaaaagcgtg atctgcttgt cgctctttcg gccgacgtcc tggccagtca
     3661 tcacgcgcca aagttccgtc acaggatgat ctggcgcgag ttgctggatc tcgccttcaa
     3721 tccgggtctg tggcgggaac tccacgaaaa tatccgaacg cagcaagata tcgcggtgca
     3781 tctcggtctt gcctgggcag tcgccgccga cgccgttgat gtggacgccg aaaaggatct
     3841 aggtgaagat cctttttgat aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc
     3901 actgagcgtc agaccccgta gaaaagatca aaggatcttc ttgagatcct ttttttctgc
     3961 gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt tgtttgccgg
     4021 atcaagagct accaactctt tttccgaagg taactggctt cagcagagcg cagataccaa
     4081 atactgttct tctagtgtag ccgtagttag gccaccactt caagaactct gtagcaccgc
     4141 ctacatacct cgctctgcta atcctgttac cagtggctgc tgccagtggc gataagtcgt
     4201 gtcttaccgg gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa
     4261 cggggggttc gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc
     4321 tacagcgtga gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc
     4381 cggtaagcgg cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct
     4441 ggtatcttta tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat
     4501 gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc
     4561 tggccttttg ctggcctttt gctcacatgt tctttcctgc gttatcccct gattctgtgg
     4621 ataaccgatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg
     4681 cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca
     4741 tctgtgcggt atttcacacc gcatatggtg cactctcagt acaatctgct ctgatgccgc
     4801 atagttaagc cagtatacac tccgctatcg ctacgtgact gggtcatggc tgcgccccga
     4861 cacccgccaa cacccgctga cgcgccctga cgggcttgtc tgctcccggc atccgcttac
     4921 agacaagctg tgaccgtctc cgggagctgc atgtgtcaga ggttttcacc gtcatcaccg
     4981 aaacgcgcga ggcaggggta cgtcgaggtc gatccaaccc ctccgctgct atagtgcagt
     5041 cggcttctga cgttcagtgc agccgtcttc tgaaaacgac atgtcgcaca agtcctaagt
     5101 tacgcgacag gctgccgccc tgcccttttc ctggcgtttt cttgtcgcgt gttttagtcg
     5161 cataaagtag aatacttgcg actagaaccg gagacattac gccatgaaca agagcgccgc
     5221 cgctggcctg ctgggctatg cccgcgtcag caccgacgac caggacttga ccaaccaacg
     5281 ggccgaactg cacgcggccg gctgcaccaa gctgttttcc gagaagatca ccggcaccag
     5341 gcgcgaccgc ccggagctgg ccaggatgct tgaccaccta cgccctggcg acgttgtgac
     5401 agtgaccagg ctagaccgcc tggcccgcag cacccgcgac ctactggaca ttgccgagcg
     5461 catccaggag gccggcgcgg gcctgcgtag cctggcagag ccgtgggccg acaccaccac
     5521 gccggccggc cgcatggtgt tgaccgtgtt cgccggcatt gccgagttcg agcgttccct
     5581 aatcatcgac cgcacccgga gcgggcgcga ggccgccaag gcgcgaggcg tgaagtttgg
     5641 cccccgccct accctcaccc cggcacagat cgcgcacgcc cgcgagctga tcgaccagga
     5701 aggccgcacc gtgaaagagg cggctgcact gcttggcgtg catcgctcga ccctgtaccg
     5761 cgcacttgag cgcagcgagg aagtgacgcc caccgaggcc aggcggcgcg gtgccttccg
     5821 tgaggacgca ttgaccgagg ccgacgccct ggcggccgcc gagaatgaac gccaagagga
     5881 acaagcatga aaccgcacca ggacggccag gacgaaccgt ttttcattac cgaagagatc
     5941 gaggcggaga tgatcgcggc cgggtacgtg ttcgagccgc ccgcgcacgt ctcaaccgtg
     6001 cggctgcatg aaatcctggc cggtttgtct gatgccaagc tcgcggcctg gccggcgagc
     6061 ttggccgctg aagaaaccga gcgccgccgt ctaaaaaggt gatgtgtatt tgagtaaaac
     6121 agcttgcgtc atgcggtcgc tgcgtatatg atgcgatgag taaataaaca aatacgcaag
     6181 gggaacgcat gaaggttatc gctgtactta accagaaagg cgggtcaggc aagacgacca
     6241 tcgcaaccca tctagcccgc gccctgcaac tcgccggggc cgatgttctg ttagtcgatt
     6301 ccgatcccca gggcagtgcc cgcgattggg cggccgtgcg ggaagatcaa ccgctaaccg
     6361 ttgtcggcat cgaccgcccg acgattgacc gcgacgtgaa ggccatcggc cggcgcgact
     6421 tcgtagtgat cgacggagcg ccccaggcgg cggacttggc tgtgtccgcg atcaaggcag
     6481 ccgacttcgt gctgattccg gtgcagccaa gcccttacga catatgggcc accgccgacc
     6541 tggtggagct ggttaagcag cgcattgagg tcacggatgg aaggctacaa gcggcctttg
     6601 tcgtgtcgcg ggcgatcaaa ggcacgcgca tcggcggtga ggttgccgag gcgctggccg
     6661 ggtacgagct gcccattctt gagtcccgta tcacgcagcg cgtgagctac ccaggcactg
     6721 ccgccgccgg cacaaccgtt cttgaatcag aacccgaggg cgacgctgcc cgcgaggtcc
     6781 aggcgctggc cgctgaaatt aaatcaaaac tcatttgagt taatgaggta aagagaaaat
     6841 gagcaaaagc acaaacacgc taagtgccgg ccgtccgagc gcacgcagca gcaaggctgc
     6901 aacgttggcc agcctggcag acacgccagc catgaagcgg gtcaactttc agttgccggc
     6961 ggaggatcac accaagctga agatgtacgc ggtacgccaa ggcaagacca ttaccgagct
     7021 gctatctgaa tacatcgcgc agctaccaga gtaaatgagc aaatgaataa atgagtagat
     7081 gaattttagc ggctaaagga ggcggcatgg aaaatcaaga acaaccaggc accgacgccg
     7141 tggaatgccc catgtgtgga ggaacgggcg gttggccagg cgtaagcggc tgggttgtct
     7201 gccggccctg caatggcact ggaaccccca agcccgagga atcggcgtga gcggtcgcaa
     7261 accatccggc ccggtacaaa tcggcgcggc gctgggtgat gacctggtgg agaagttgaa
     7321 ggcggcgcag gccgcccagc ggcaacgcat cgaggcagaa gcacgccccg gtgaatcgtg
     7381 gcaagcggcc gctgatcgaa tccgcaaaga atcccggcaa ccgccggcag ccggtgcgcc
     7441 gtcgattagg aagccgccca agggcgacga gcaaccagat tttttcgttc cgatgctcta
     7501 tgacgtgggc acccgcgata gtcgcagcat catggacgtg gccgttttcc gtctgtcgaa
     7561 gcgtgaccga cgagctggcg aggtgatccg ctacgagctt ccagacgggc acgtagaggt
     7621 ttccgcaggg ccggccggca tggcgagtgt gtgggattac gacctggtac tgatggcggt
     7681 ttcccatcta accgaatcca tgaaccgata ccgggaaggg aagggagaca agcccggccg
     7741 cgtgttccgt ccacacgttg cggacgtact caagttctgc cggcgagccg atggcggaaa
     7801 gcagaaagac gacctggtag aaacctgcat tcggttaaac accacgcacg ttgccatgca
     7861 gcgtacgaag aaggccaaga acggccgcct ggtgacggta tccgagggtg aagccttgat
     7921 tagccgctac aagatcgtaa agagcgaaac cgggcggccg gagtacatcg agatcgagct
     7981 agctgattgg atgtaccgcg agatcacaga aggcaagaac ccggacgtgc tgacggttca
     8041 ccccgattac tttttgatcg atcccggcat cggccgtttt ctctaccgcc tggcacgccg
     8101 cgccgcaggc aaggcagaag ccagatggtt gttcaagacg atctacgaac gcagtggcag
     8161 cgccggagag ttcaagaagt tctgtttcac cgtgcgcaag ctgatcgggt caaatgacct
     8221 gccggagtac gatttgaagg aggaggcggg gcaggctggc ccgatcctag tcatgcgcta
     8281 ccgcaacctg atcgagggcg aagcatccgc cggttcctaa tgtacggagc agatgctagg
     8341 gcaaattgcc ctagcagggg aaaaaggtcg aaaaggtctc tttcctgtgg atagcacgta
     8401 cattgggaac ccaaagccgt acattgggaa ccggaacccg tacattggga acccaaagcc
     8461 gtacattggg aaccggtcac acatgtaagt gactgatata aaagagaaaa aaggcgattt
     8521 ttccgcctaa aactctttaa aacttattaa aactcttaaa acccgcctgg cctgtgcata
     8581 actgtctggc cagcgcacag ccgaagagct gcaaaaagcg cctacccttc ggtcgctgcg
     8641 ctccctacgc cccgccgctt cgcgtcggcc tatcgcggcc gctggccgct caaaaatggc
     8701 tggcctacgg ccaggcaatc taccagggcg cggacaagcc gcgccgtcgc cactcgaccg
     8761 ccggcgccca catcaaggca ccggtgggta tgcctgacga tgcgtggaga ccgaaacctt
     8821 gcgctcgttc gccagccagg acagaaatgc ctcgacttcg ctgctgccca aggttgccgg
     8881 gtgacgcaca ccgtggaaac ggatgaaggc acgaacccag tggacataag cctgttcggt
     8941 tcgtaagctg taatgcaagt agcgtatgcg ctcacgcaac tggtccagaa ccttgaccga
     9001 acgcagcggt ggtaacggcg cagtggcggt tttcatggct tgttatgact gtttttttgg
     9061 ggtacagtct atgcctcggg catccaagca gcaagcgcgt tacgccgtgg gtcgatgttt
     9121 gatgttatgg agcagcaacg atgttacgca gcagggcagt cgccctaaaa caaagttaaa
     9181 catcatgagg gaagcggtga tcgccgaagt atcgactcaa ctatcagagg tagttggcgt
     9241 catcgagcgc catctcgaac cgacgttgct ggccgtacat ttgtacggct ccgcagtgga
     9301 tggcggcctg aagccacaca gtgatattga tttgctggtt acggtgaccg taaggcttga
     9361 tgaaacaacg cggcgagctt tgatcaacga ccttttggaa acttcggctt cccctggaga
     9421 gagcgagatt ctccgcgctg tagaagtcac cattgttgtg cacgacgaca tcattccgtg
     9481 gcgttatcca gctaagcgcg aactgcaatt tggagaatgg cagcgcaatg acattcttgc
     9541 aggtatcttc gagccagcca cgatcgacat tgatctggct atcttgctga caaaagcaag
     9601 agaacatagc gttgccttgg taggtccagc ggcggaggaa ctctttgatc cggttcctga
     9661 acaggatcta tttgaggcgc taaatgaaac cttaacgcta tggaactcgc cgcccgactg
     9721 ggctggcgat gagcgaaatg tagtgcttac gttgtcccgc atttggtaca gcgcagtaac
     9781 cggcaaaatc gcgccgaagg atgtcgctgc cgactgggca atggagcgcc tgccggccca
     9841 gtatcagccc gtcatacttg aagctagaca ggcttatctt ggacaagaag aagatcgctt
     9901 ggcctcgcgc gcagatcagt tggaagaatt tgtccactac gtgaaaggcg agatcaccaa
     9961 ggtagtcggc aaataatgtc taacaattcg ttcaagccga cgccgcttcg cggcgcggct
    10021 taactcaagc gttagatgca ctaagcacat aattgctcac agccaaacta tcaggtcaag
    10081 tctgctttta ttatttttaa gcgtgcataa taagccctac acaaattggg agatatatca
    10141 tgaaaggctg gctttttctt gttatcgcaa tagttggcga agtaatcgca acatagcttg
    10201 cttggtcgtt ccgcgtgaac gtcggctcga ttgtacctgc gttcaaatac tttgcgatcg
    10261 tgttgcgcgc ctgcccggtg cgtcggctga tctcacggat cgactgcttc tctcgcaacg
    10321 ccatccgacg gatgatgttt aaaagtccca tgtggatcac tccgttgccc cgtcgctcac
    10381 cgtgttgggg ggaaggtgca catggctcag ttctcaatgg aaattatctg cctaaccggc
    10441 tcagttctgc gtagaaacca acatgcaagc tccaccgggt gcaaagcggc agcggc
//
