LOCUS       B594p2x35s-mGFPM        4867 bp    DNA     circular SYN 22-JAN-2016
DEFINITION  Ligation of B442pmGFPM-ocs* into B496p2x35s-MoGusi-ocs*
ACCESSION   B594p2x35s-mGFPM
KEYWORDS    .
SOURCE      Unknown.
  ORGANISM  Unknown
            Unclassified.
REFERENCE   1  (bases 1 to 4867)
  AUTHORS   Self
  JOURNAL   Unpublished.
COMMENT     SECID/File created by Clone Manager, Scientific & Educational Software
COMMENT     SECNOTES|Vector molecule:  B496p2x35s-MoGusi-ocs*
               Fragment ends: SalI and HindIII
               Fragment size: 3935
            Insert molecule:  B442pmGFPM-ocs*
               Fragment ends: HindIII and SalI
               Fragment size: 932
            GELR
            Sequencing primers:
            M13RY
            OR2	GTGCACAACAGAATTGAAAGCAAA
FEATURES             Location/Qualifiers
     misc_feature    129..913
                     /gene="P2x35s"
                     /SECDrawAs="Region"
                     /SECStyleId=1
     misc_signal     743
                     /label=mut 
                     /SECDrawAs="Label"
     misc_signal     841..847
                     /label=TATA
                     /SECDrawAs="Label"
     CDS             942..1320
                     /gene="gfp"
                     /product="GFP"
                     /SECDrawAs="Gene"
                     /SECStyleId=1
     CDS             1321..1509
                     /gene="int"
                     /product="STLS1"
                     /SECDrawAs="Gene"
                     /SECStyleId=1
     CDS             1510..1847
                     /gene="gfp"
                     /product="GFP"
                     /SECDrawAs="Gene"
                     /SECStyleId=1
     misc_feature    1856..2051
                     /gene="Tocs"
                     /product="Terminator"
                     /SECDrawAs="Region"
                     /SECStyleId=1
                     /SECName="Tocs"
                     /SECDescr="Terminator"
     misc_feature    2867..3727
                     /gene="Amp"
                     /product="ORF-1"
                     /SECDrawAs="Gene"
                     /SECStyleId=1
                     /SECName="Amp"
                     /SECDescr="ORF-1"
     CDS             3770..4486
                     /gene="ColE1"
                     /product="ColE1"
                     /SECDrawAs="Region"
                     /SECStyleId=1
                     /SECName="ColE1"
                     /SECDescr="ColE1"
ORIGIN      
        1 cacacaggaa acagctatga ccatgattac gccaagctcg aaattaaccc tcactaaagg
       61 gaacaaaagc tggagctctc tagaggccat ggcggccgca ctagtggatc ccccgggcta
      121 gatcgggcca acatggtgga gcacgacact ctcgtctact ccaagaatat caaagataca
      181 gtctcagaag accaaagggc tattgagact tttcaacaaa gggtaatatc gggaaacctc
      241 ctcggattcc attgcccagc tatctgtcac ttcatcaaaa ggacagtaga aaaggaaggt
      301 ggcacctaca aatgccatca ttgcgataaa ggaaaggcta tcgttcaaga tgcctctgcc
      361 gacagtggtc ccaaagatgg acccccaccc acgaggagca tcgtggaaaa agaagacgtt
      421 ccaaccacgt cttcaaagca agtggattga tgtgaacatg gtggagcacg acactctcgt
      481 ctactccaag aatatcaaag atacagtctc agaagaccaa agggctattg agacttttca
      541 acaaagggta atatcgggaa acctcctcgg attccattgc ccagctatct gtcacttcat
      601 caaaaggaca gtagaaaagg aaggtggcac ctacaaatgc catcattgcg ataaaggaaa
      661 ggctatcgtt caagatgcct ctgccgacag tggtcccaaa gatggacccc cacccacgag
      721 gagcatcgtg gaaaaagaag accttccaac cacgtcttca aagcaagtgg attgatgtga
      781 tatctccact gacgtaaggg atgacgcaca atcccactat ccttcgcaag acccttcctc
      841 tatataagga agttcatttc atttggagag gacacgctga aatcaccagt ctctctctac
      901 aaatctatct ctctcgacaa gcttacgcgt cctccaccga aatgggcaag ggcgaggaac
      961 tgttcactgg cgtggtccca atcctggtgg aactggatgg tgatgtgaac gggcacaagt
     1021 tctccgtcag cggagagggt gaaggtgatg ccacctacgg aaagctcacc ctgaagttca
     1081 tctgcactac cggaaagctc cctgttccgt ggccaaccct cgtcaccact ttcacctacg
     1141 gtgttcagtg cttctcccgg tacccagatc acatgaagca gcatgacttc ttcaagagcg
     1201 ccatgcccga aggctacgtg caagaaagga ctatcttctt caaggatgac gggaactaca
     1261 agacacgtgc cgaagtcaag ttcgaaggtg ataccctggt gaaccgcatc gagctgaaag
     1321 gtaagtttct gcttctacct ttgatatata tataataatt atcattaatt agtagtaata
     1381 taatatttca aatatttttt tcaaaataaa agaatgtagt atatagcaat tgcttttctg
     1441 tagtttataa gtgtgtatat tttaatttat aacttttcta atatatgacc aaaatttgtt
     1501 gatgtgcagg tatcgatttc aaggaagatg gaaacatcct cggacacaag ctggagtaca
     1561 actacaactc ccacaacgta tacatcatgg ccgacaagca gaagaacggc atcaaggtga
     1621 acttcaagat caggcacaac atcgaagatg gaagcgtgca actggcggac cactaccagc
     1681 agaacacgcc catcggcgat ggccctgtcc tgctgccgga caaccattac ctgtccacgc
     1741 aatctgccct ctccaaggac cccaacgaga agagggacca catggtcctg ctggagttcg
     1801 tgacggctgc tgggatcacg catggcatgg atgaactcta caagtgactc gtcgaccctg
     1861 ctttaatgag atatgcgaga cgcctatgat cgcatgatat ttgctttcaa ttctgttgtg
     1921 cacgttgtaa aaaacctgag catgtgtagc tcagatcctt accgccggtt tcggttcatt
     1981 ctaatgaata tatcacccgt tactatcgta tttttatgaa taatattctc cgttcaattt
     2041 actgattgtc ctcgacaggc cttaagggcc agatcttggg cccggtaccc aattcgccct
     2101 atagtgagtc gtattacaat tcactggccg tcgttttaca acgtcgtgac tgggaaaacc
     2161 ctggcgttac ccaacttaat cgccttgcag cacatccccc tttcgccagc tggcgtaata
     2221 gcgaagaggc ccgcaccgat cgcccttccc aacagttgcg cagcctgaat ggcgaatgga
     2281 aattgtaagc gttaatattt tgttaaaatt cgcgttaaat ttttgttaaa tcagctcatt
     2341 ttttaaccaa taggccgaaa tcggcaaaat cccttataaa tcaaaagaat agaccgagat
     2401 agggttgagt gttgttccag tttggaacaa gagtccacta ttaaagaacg tggactccaa
     2461 cgtcaaaggg cgaaaaaccg tctatcaggg cgatggccca ctacgtgaac catcacccta
     2521 atcaagtttt ttggggtcga ggtgccgtaa agcactaaat cggaacccta aagggagccc
     2581 ccgatttaga gcttgacggg gaaagccggc gaacgtggcg agaaaggaag ggaagaaagc
     2641 gaaaggagcg ggcgctaggg cgctggcaag tgtagcggtc acgctgcgcg taaccaccac
     2701 acccgccgcg cttaatgcgc cgctacaggg cgcgtcaggt ggcacttttc ggggaaatgt
     2761 gcgcggaacc cctatttgtt tatttttcta aatacattca aatatgtatc cgctcatgag
     2821 acaataaccc tgataaatgc ttcaataata ttgaaaaagg aagagtatga gtattcaaca
     2881 tttccgtgtc gcccttattc ccttttttgc ggcattttgc cttcctgttt ttgctcaccc
     2941 agaaacgctg gtgaaagtaa aagatgctga agatcagttg ggtgcacgag tgggttacat
     3001 cgaactggat ctcaacagcg gtaagatcct tgagagtttt cgccccgaag aacgttttcc
     3061 aatgatgagc acttttaaag ttctgctatg tggcgcggta ttatcccgta ttgacgccgg
     3121 gcaagagcaa ctcggtcgcc gcatacacta ttctcagaat gacttggttg agtactcacc
     3181 agtcacagaa aagcatctta cggatggcat gacagtaaga gaattatgca gtgctgccat
     3241 aaccatgagt gataacactg cggccaactt acttctgaca acgatcggag gaccgaagga
     3301 gctaaccgct tttttgcaca acatggggga tcatgtaact cgccttgatc gttgggaacc
     3361 ggagctgaat gaagccatac caaacgacga gcgtgacacc acgatgcctg tagcaatggc
     3421 aacaacgttg cgcaaactat taactggcga actacttact ctagcttccc ggcaacaatt
     3481 aatagactgg atggaggcgg ataaagttgc aggaccactt ctgcgctcgg cccttccggc
     3541 tggctggttt attgctgata aatctggagc cggtgagcgt gggtctcgcg gtatcattgc
     3601 agcactgggg ccagatggta agccctcccg tatcgtagtt atctacacga cggggagtca
     3661 ggcaactatg gatgaacgaa atagacagat cgctgagata ggtgcctcac tgattaagca
     3721 ttggtaactg tcagaccaag tttactcata tatactttag attgatttaa aacttcattt
     3781 ttaatttaaa aggatctagg tgaagatcct ttttgataat ctcatgacca aaatccctta
     3841 acgtgagttt tcgttccact gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg
     3901 agatcctttt tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac cgctaccagc
     3961 ggtggtttgt ttgccggatc aagagctacc aactcttttt ccgaaggtaa ctggcttcag
     4021 cagagcgcag ataccaaata ctgtccttct agtgtagccg tagttaggcc accacttcaa
     4081 gaactctgta gcaccgccta catacctcgc tctgctaatc ctgttaccag tggctgctgc
     4141 cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac cggataaggc
     4201 gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc agcttggagc gaacgaccta
     4261 caccgaactg agatacctac agcgtgagct atgagaaagc gccacgcttc ccgaagggag
     4321 aaaggcggac aggtatccgg taagcggcag ggtcggaaca ggagagcgca cgagggagct
     4381 tccaggggga aacgcctggt atctttatag tcctgtcggg tttcgccacc tctgacttga
     4441 gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc
     4501 ggccttttta cggttcctgg ccttttgctg gccttttgct cacatgttct ttcctgcgtt
     4561 atcccctgat tctgtggata accgtattac cgcctttgag tgagctgata ccgctcgccg
     4621 cagccgaacg accgagcgca gcgagtcagt gagcgaggaa gcggaagagc gcccaatacg
     4681 caaaccgcct ctccccgcgc gttggccgat tcattaatgc agctggcacg acaggtttcc
     4741 cgactggaaa gcgggcagtg agcgcaacgc aattaatgtg agttagctca ctcattaggc
     4801 accccaggct ttacacttta tgcttccggc tcgtatgttg tgtggaattg tgagcggata
     4861 acaattt
//
