LOCUS       B595pUbiZm-mGFPM        5580 bp    DNA     circular SYN 22-JAN-2016
DEFINITION  Ligation of B442pmGFPM-ocs* into B497pUbiZm-MoGusi-ocs*
ACCESSION   B595pUbiZm-mGFPM
KEYWORDS    .
SOURCE      Unknown.
  ORGANISM  Unknown
            Unclassified.
REFERENCE   1  (bases 1 to 5580)
  AUTHORS   Self
  JOURNAL   Unpublished.
COMMENT     SECID/File created by Clone Manager, Scientific & Educational Software
COMMENT     SECNOTES|Vector molecule:  B497pUbiZm-MoGusi-ocs*
               Fragment ends: SalI and HindIII
               Fragment size: 4648
            Insert molecule:  B442pmGFPM-ocs*
               Fragment ends: HindIII and SalI
               Fragment size: 932
            GELR
            Sequencing primers	
            OR2	GTGCACAACAGAATTGAAAGCAAA     weglassen
            UF2
FEATURES             Location/Qualifiers
     misc_feature    122..1629
                     /gene="UbiZm"
                     /product="Promotor"
                     /SECDrawAs="Region"
                     /SECStyleId=1
     misc_feature    1108..1627
                     /gene="int"
                     /product="Intron"
                     /SECDrawAs="Region"
                     /SECStyleId=1
     CDS             1655..2033
                     /gene="gfp"
                     /product="GFP"
                     /SECDrawAs="Gene"
                     /SECStyleId=1
     CDS             2034..2222
                     /gene="int"
                     /product="STLS1"
                     /SECDrawAs="Gene"
                     /SECStyleId=1
     CDS             2223..2560
                     /gene="gfp"
                     /product="GFP"
                     /SECDrawAs="Gene"
                     /SECStyleId=1
     misc_feature    2569..2764
                     /gene="Tocs"
                     /product="Terminator"
                     /SECDrawAs="Region"
                     /SECStyleId=1
                     /SECName="Tocs"
                     /SECDescr="Terminator"
     misc_feature    3580..4440
                     /gene="Amp"
                     /product="ORF-1"
                     /SECDrawAs="Gene"
                     /SECStyleId=1
                     /SECName="Amp"
                     /SECDescr="ORF-1"
     CDS             4483..5199
                     /gene="ColE1"
                     /product="ColE1"
                     /SECDrawAs="Region"
                     /SECStyleId=1
                     /SECName="ColE1"
                     /SECDescr="ColE1"
ORIGIN      
        1 cacacaggaa acagctatga ccatgattac gccaagctcg aaattaaccc tcactaaagg
       61 gaacaaaagc tggagctctc tagaggccat ggcggccgca ctagtggatc ccccgggtac
      121 gtgcagtgca gcgtgacccg gtcgtgcccc tctctagaga taatgagcat tgcatgtcta
      181 agttataaaa aattaccaca tatttttttt gtcacacttg tttgaagtgc agtttatcta
      241 tctttataca tatatttaaa ctttactcta cgaataatat aatctatagt actacaataa
      301 tatcagtgtt ttagagaatc atataaatga acagttagac atggtctaaa ggacaattga
      361 gtattttgac aacaggactc tacagtttta tctttttagt gtgcatgtgt tctccttttt
      421 ttttgcaaat agcttcacct atataatact tcatccattt tattagtaca tccatttagg
      481 gtttagggtt aatggttttt atagactaat ttttttagta catctatttt attctatttt
      541 agcctctaaa ttaagaaaac taaaactcta ttttagtttt tttatttaat aatttagata
      601 taaaatagaa taaaataaag tgactaaaaa ttaaacaaat accctttaag aaattaaaaa
      661 aactaaggaa acatttttct tgtttcgagt agataatgcc agcctgttaa acgccgtcga
      721 tcgacgagtc taacggacac caaccagcga accagcagcg tcgcgtcggg ccaagcgaag
      781 cagacggcac ggcatctctg tcgctgcctc tggacccctc tcgagagttc cgctccaccg
      841 ttggacttgc tccgctgtcg gcatccagaa attgcgtggc ggagcggcag acgtgagccg
      901 gcacggcagg cggcctcctc ctcctctcac ggcaccggca gctacggggg attcctttcc
      961 caccgctcct tcgctttccc ttcctcgccc gccgtaataa atagacaccc cctccacacc
     1021 ctctttcccc aacctcgtgt tgttcggagc gcacacacac acaaccagat ctcccccaaa
     1081 tccacccgtc ggcacctccg cttcaaggta cgccgctcgt cctccccccc cccccctctc
     1141 taccttctct agatcggcgt tccggtccat ggttagggcc cggtagttct acttctgttc
     1201 atgtttgtgt tagatccgtg tttgtgttag atccgtgctg ctagcgttcg tacacggatg
     1261 cgacctgtac gtcagacacg ttctgattgc taacttgcca gtgtttctct ttggggaatc
     1321 ctgggatggc tctagccgtt ccgcagacgg gatcgatcta ggataggtat acatgttgat
     1381 gtgggtttta ctgatgcata tacatgatgg catatgcagc atctattcat atgctctaac
     1441 cttgagtacc tatctattat aataaacaag tatgttttat aattattttg atcttgatat
     1501 acttggatga tggcatatgc agcagctata tgtggatttt tttagccctg ccttcatacg
     1561 ctatttattt gcttggtact gtttcttttg tcgatgctca ccctgttgtt tggtgttact
     1621 tctgcaggta caagcttacg cgtcctccac cgaaatgggc aagggcgagg aactgttcac
     1681 tggcgtggtc ccaatcctgg tggaactgga tggtgatgtg aacgggcaca agttctccgt
     1741 cagcggagag ggtgaaggtg atgccaccta cggaaagctc accctgaagt tcatctgcac
     1801 taccggaaag ctccctgttc cgtggccaac cctcgtcacc actttcacct acggtgttca
     1861 gtgcttctcc cggtacccag atcacatgaa gcagcatgac ttcttcaaga gcgccatgcc
     1921 cgaaggctac gtgcaagaaa ggactatctt cttcaaggat gacgggaact acaagacacg
     1981 tgccgaagtc aagttcgaag gtgataccct ggtgaaccgc atcgagctga aaggtaagtt
     2041 tctgcttcta cctttgatat atatataata attatcatta attagtagta atataatatt
     2101 tcaaatattt ttttcaaaat aaaagaatgt agtatatagc aattgctttt ctgtagttta
     2161 taagtgtgta tattttaatt tataactttt ctaatatatg accaaaattt gttgatgtgc
     2221 aggtatcgat ttcaaggaag atggaaacat cctcggacac aagctggagt acaactacaa
     2281 ctcccacaac gtatacatca tggccgacaa gcagaagaac ggcatcaagg tgaacttcaa
     2341 gatcaggcac aacatcgaag atggaagcgt gcaactggcg gaccactacc agcagaacac
     2401 gcccatcggc gatggccctg tcctgctgcc ggacaaccat tacctgtcca cgcaatctgc
     2461 cctctccaag gaccccaacg agaagaggga ccacatggtc ctgctggagt tcgtgacggc
     2521 tgctgggatc acgcatggca tggatgaact ctacaagtga ctcgtcgacc ctgctttaat
     2581 gagatatgcg agacgcctat gatcgcatga tatttgcttt caattctgtt gtgcacgttg
     2641 taaaaaacct gagcatgtgt agctcagatc cttaccgccg gtttcggttc attctaatga
     2701 atatatcacc cgttactatc gtatttttat gaataatatt ctccgttcaa tttactgatt
     2761 gtcctcgaca ggccttaagg gccagatctt gggcccggta cccaattcgc cctatagtga
     2821 gtcgtattac aattcactgg ccgtcgtttt acaacgtcgt gactgggaaa accctggcgt
     2881 tacccaactt aatcgccttg cagcacatcc ccctttcgcc agctggcgta atagcgaaga
     2941 ggcccgcacc gatcgccctt cccaacagtt gcgcagcctg aatggcgaat ggaaattgta
     3001 agcgttaata ttttgttaaa attcgcgtta aatttttgtt aaatcagctc attttttaac
     3061 caataggccg aaatcggcaa aatcccttat aaatcaaaag aatagaccga gatagggttg
     3121 agtgttgttc cagtttggaa caagagtcca ctattaaaga acgtggactc caacgtcaaa
     3181 gggcgaaaaa ccgtctatca gggcgatggc ccactacgtg aaccatcacc ctaatcaagt
     3241 tttttggggt cgaggtgccg taaagcacta aatcggaacc ctaaagggag cccccgattt
     3301 agagcttgac ggggaaagcc ggcgaacgtg gcgagaaagg aagggaagaa agcgaaagga
     3361 gcgggcgcta gggcgctggc aagtgtagcg gtcacgctgc gcgtaaccac cacacccgcc
     3421 gcgcttaatg cgccgctaca gggcgcgtca ggtggcactt ttcggggaaa tgtgcgcgga
     3481 acccctattt gtttattttt ctaaatacat tcaaatatgt atccgctcat gagacaataa
     3541 ccctgataaa tgcttcaata atattgaaaa aggaagagta tgagtattca acatttccgt
     3601 gtcgccctta ttcccttttt tgcggcattt tgccttcctg tttttgctca cccagaaacg
     3661 ctggtgaaag taaaagatgc tgaagatcag ttgggtgcac gagtgggtta catcgaactg
     3721 gatctcaaca gcggtaagat ccttgagagt tttcgccccg aagaacgttt tccaatgatg
     3781 agcactttta aagttctgct atgtggcgcg gtattatccc gtattgacgc cgggcaagag
     3841 caactcggtc gccgcataca ctattctcag aatgacttgg ttgagtactc accagtcaca
     3901 gaaaagcatc ttacggatgg catgacagta agagaattat gcagtgctgc cataaccatg
     3961 agtgataaca ctgcggccaa cttacttctg acaacgatcg gaggaccgaa ggagctaacc
     4021 gcttttttgc acaacatggg ggatcatgta actcgccttg atcgttggga accggagctg
     4081 aatgaagcca taccaaacga cgagcgtgac accacgatgc ctgtagcaat ggcaacaacg
     4141 ttgcgcaaac tattaactgg cgaactactt actctagctt cccggcaaca attaatagac
     4201 tggatggagg cggataaagt tgcaggacca cttctgcgct cggcccttcc ggctggctgg
     4261 tttattgctg ataaatctgg agccggtgag cgtgggtctc gcggtatcat tgcagcactg
     4321 gggccagatg gtaagccctc ccgtatcgta gttatctaca cgacggggag tcaggcaact
     4381 atggatgaac gaaatagaca gatcgctgag ataggtgcct cactgattaa gcattggtaa
     4441 ctgtcagacc aagtttactc atatatactt tagattgatt taaaacttca tttttaattt
     4501 aaaaggatct aggtgaagat cctttttgat aatctcatga ccaaaatccc ttaacgtgag
     4561 ttttcgttcc actgagcgtc agaccccgta gaaaagatca aaggatcttc ttgagatcct
     4621 ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt
     4681 tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt cagcagagcg
     4741 cagataccaa atactgtcct tctagtgtag ccgtagttag gccaccactt caagaactct
     4801 gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc tgccagtggc
     4861 gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa ggcgcagcgg
     4921 tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac ctacaccgaa
     4981 ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg
     5041 gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga gcttccaggg
     5101 ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact tgagcgtcga
     5161 tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa cgcggccttt
     5221 ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc gttatcccct
     5281 gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg ccgcagccga
     5341 acgaccgagc gcagcgagtc agtgagcgag gaagcggaag agcgcccaat acgcaaaccg
     5401 cctctccccg cgcgttggcc gattcattaa tgcagctggc acgacaggtt tcccgactgg
     5461 aaagcgggca gtgagcgcaa cgcaattaat gtgagttagc tcactcatta ggcaccccag
     5521 gctttacact ttatgcttcc ggctcgtatg ttgtgtggaa ttgtgagcgg ataacaattt
//
