LOCUS B596pActOs-mGFPM 5465 bp DNA circular SYN 22-JAN-2016 DEFINITION Ligation of B442pmGFPM-ocs* into B498pActOs-MoGusi-ocs* ACCESSION B596pActOs-mGFPM KEYWORDS . SOURCE Unknown. ORGANISM Unknown Unclassified. REFERENCE 1 (bases 1 to 5465) AUTHORS Self JOURNAL Unpublished. COMMENT SECID/File created by Clone Manager, Scientific & Educational Software COMMENT SECNOTES|Vector molecule: B498pActOs-MoGusi-ocs* Fragment ends: SalI and HindIII Fragment size: 4533 Insert molecule: B442pmGFPM-ocs* Fragment ends: HindIII and SalI Fragment size: 932 GELR Sequencing primers OR2 GTGCACAACAGAATTGAAAGCAAA M13RY FEATURES Location/Qualifiers misc_feature 118..1514 /gene="ACT" /product="Actin Promotor" /SECDrawAs="Region" /SECStyleId=1 CDS 1540..1918 /gene="gfp" /product="GFP" /SECDrawAs="Gene" /SECStyleId=1 CDS 1919..2107 /gene="int" /product="STLS1" /SECDrawAs="Gene" /SECStyleId=1 CDS 2108..2445 /gene="gfp" /product="GFP" /SECDrawAs="Gene" /SECStyleId=1 misc_feature 2454..2649 /gene="Tocs" /product="Terminator" /SECDrawAs="Region" /SECStyleId=1 /SECName="Tocs" /SECDescr="Terminator" misc_feature 3465..4325 /gene="Amp" /product="ORF-1" /SECDrawAs="Gene" /SECStyleId=1 /SECName="Amp" /SECDescr="ORF-1" CDS 4368..5084 /gene="ColE1" /product="ColE1" /SECDrawAs="Region" /SECStyleId=1 /SECName="ColE1" /SECDescr="ColE1" ORIGIN 1 cacacaggaa acagctatga ccatgattac gccaagctcg aaattaaccc tcactaaagg 61 gaacaaaagc tggagctctc tagaggccat ggcggccgca ctagtggatc ccccgggtcg 121 aggtcattca tatgcttgag aagagagtcg ggatagtcca aaataaaaca aaggtaagat 181 tacctggtca aaagtgaaaa catcagttaa aaggtggtat aaagtaaaat atcggtaata 241 aaaggtggcc caaagtgaaa tttactcttt tctactatta taaaaattga ggatgttttt 301 gtcggtactt tgatacgtca tttttgtatg aattggtttt taagtttatt cgcttttgga 361 aatgcatatc tgtatttgag tcgggtttta agttcgtttg cttttgtaaa tacagaggga 421 tttgtataag aaatatcttt aaaaaaaccc atatgctaat ttgacataat ttttgagaaa 481 aatatatatt caggcgaatt ctcacaatga acaataataa gattaaaata gctttccccc 541 gttgcagcgc atgggtattt tttctagtaa aaataaaaga taaacttaga ctcaaaacat 601 ttacaaaaac aacccctaaa gttcctaaag cccaaagtgc tatccacgat ccatagcaag 661 cccagcccaa cccaacccaa cccaacccac cccagtccag ccaactggac aatagtctcc 721 acaccccccc actatcaccg tgagttgtcc gcacgcaccg cacgtctcgc agccaaaaaa 781 aaaaaaagaa agaaaaaaaa gaaaaagaaa aaacagcagg tgggtccggg tcgtgggggc 841 cggaaacgcg aggaggatcg cgagccagcg acgaggccgg ccctccctcc gcttccaaag 901 aaacgccccc catcgccact atatacatac ccccccctct cctcccatcc ccccaaccct 961 accaccacca ccaccaccac ctccacctcc tcccccctcg ctgccggacg acgagctcct 1021 cccccctccc cctccgccgc cgccgcgccg gtaaccaccc cgcccctctc ctctttcttt 1081 ctccgttttt tttttccgtc tcggtctcga tctttggcct tggtagtttg ggtgggcgag 1141 aggcggcttc gtgcgcgccc agatcggtgc gcgggagggg cgggatctcg cggctggggc 1201 tctcgccggc gtggatccgg cccggatctc gcggggaatg gggctctcgg atgtagatct 1261 gcgatccgcc gttgttgggg gagatgatgg ggggtttaaa atttccgcca tgctaaacaa 1321 gatcaggaag aggggaaaag ggcactatgg tttatatttt tatatatttc tgctgcttcg 1381 tcaggcttag atgtgctaga tctttctttc ttctttttgt gggtagaatt tgaatccctc 1441 agcattgttc atcggtagtt tttcttttca tgatttgtga caaatgcagc ctcgtgcgga 1501 gcttttttgt aggtacaagc ttacgcgtcc tccaccgaaa tgggcaaggg cgaggaactg 1561 ttcactggcg tggtcccaat cctggtggaa ctggatggtg atgtgaacgg gcacaagttc 1621 tccgtcagcg gagagggtga aggtgatgcc acctacggaa agctcaccct gaagttcatc 1681 tgcactaccg gaaagctccc tgttccgtgg ccaaccctcg tcaccacttt cacctacggt 1741 gttcagtgct tctcccggta cccagatcac atgaagcagc atgacttctt caagagcgcc 1801 atgcccgaag gctacgtgca agaaaggact atcttcttca aggatgacgg gaactacaag 1861 acacgtgccg aagtcaagtt cgaaggtgat accctggtga accgcatcga gctgaaaggt 1921 aagtttctgc ttctaccttt gatatatata taataattat cattaattag tagtaatata 1981 atatttcaaa tatttttttc aaaataaaag aatgtagtat atagcaattg cttttctgta 2041 gtttataagt gtgtatattt taatttataa cttttctaat atatgaccaa aatttgttga 2101 tgtgcaggta tcgatttcaa ggaagatgga aacatcctcg gacacaagct ggagtacaac 2161 tacaactccc acaacgtata catcatggcc gacaagcaga agaacggcat caaggtgaac 2221 ttcaagatca ggcacaacat cgaagatgga agcgtgcaac tggcggacca ctaccagcag 2281 aacacgccca tcggcgatgg ccctgtcctg ctgccggaca accattacct gtccacgcaa 2341 tctgccctct ccaaggaccc caacgagaag agggaccaca tggtcctgct ggagttcgtg 2401 acggctgctg ggatcacgca tggcatggat gaactctaca agtgactcgt cgaccctgct 2461 ttaatgagat atgcgagacg cctatgatcg catgatattt gctttcaatt ctgttgtgca 2521 cgttgtaaaa aacctgagca tgtgtagctc agatccttac cgccggtttc ggttcattct 2581 aatgaatata tcacccgtta ctatcgtatt tttatgaata atattctccg ttcaatttac 2641 tgattgtcct cgacaggcct taagggccag atcttgggcc cggtacccaa ttcgccctat 2701 agtgagtcgt attacaattc actggccgtc gttttacaac gtcgtgactg ggaaaaccct 2761 ggcgttaccc aacttaatcg ccttgcagca catccccctt tcgccagctg gcgtaatagc 2821 gaagaggccc gcaccgatcg cccttcccaa cagttgcgca gcctgaatgg cgaatggaaa 2881 ttgtaagcgt taatattttg ttaaaattcg cgttaaattt ttgttaaatc agctcatttt 2941 ttaaccaata ggccgaaatc ggcaaaatcc cttataaatc aaaagaatag accgagatag 3001 ggttgagtgt tgttccagtt tggaacaaga gtccactatt aaagaacgtg gactccaacg 3061 tcaaagggcg aaaaaccgtc tatcagggcg atggcccact acgtgaacca tcaccctaat 3121 caagtttttt ggggtcgagg tgccgtaaag cactaaatcg gaaccctaaa gggagccccc 3181 gatttagagc ttgacgggga aagccggcga acgtggcgag aaaggaaggg aagaaagcga 3241 aaggagcggg cgctagggcg ctggcaagtg tagcggtcac gctgcgcgta accaccacac 3301 ccgccgcgct taatgcgccg ctacagggcg cgtcaggtgg cacttttcgg ggaaatgtgc 3361 gcggaacccc tatttgttta tttttctaaa tacattcaaa tatgtatccg ctcatgagac 3421 aataaccctg ataaatgctt caataatatt gaaaaaggaa gagtatgagt attcaacatt 3481 tccgtgtcgc ccttattccc ttttttgcgg cattttgcct tcctgttttt gctcacccag 3541 aaacgctggt gaaagtaaaa gatgctgaag atcagttggg tgcacgagtg ggttacatcg 3601 aactggatct caacagcggt aagatccttg agagttttcg ccccgaagaa cgttttccaa 3661 tgatgagcac ttttaaagtt ctgctatgtg gcgcggtatt atcccgtatt gacgccgggc 3721 aagagcaact cggtcgccgc atacactatt ctcagaatga cttggttgag tactcaccag 3781 tcacagaaaa gcatcttacg gatggcatga cagtaagaga attatgcagt gctgccataa 3841 ccatgagtga taacactgcg gccaacttac ttctgacaac gatcggagga ccgaaggagc 3901 taaccgcttt tttgcacaac atgggggatc atgtaactcg ccttgatcgt tgggaaccgg 3961 agctgaatga agccatacca aacgacgagc gtgacaccac gatgcctgta gcaatggcaa 4021 caacgttgcg caaactatta actggcgaac tacttactct agcttcccgg caacaattaa 4081 tagactggat ggaggcggat aaagttgcag gaccacttct gcgctcggcc cttccggctg 4141 gctggtttat tgctgataaa tctggagccg gtgagcgtgg gtctcgcggt atcattgcag 4201 cactggggcc agatggtaag ccctcccgta tcgtagttat ctacacgacg gggagtcagg 4261 caactatgga tgaacgaaat agacagatcg ctgagatagg tgcctcactg attaagcatt 4321 ggtaactgtc agaccaagtt tactcatata tactttagat tgatttaaaa cttcattttt 4381 aatttaaaag gatctaggtg aagatccttt ttgataatct catgaccaaa atcccttaac 4441 gtgagttttc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag 4501 atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg 4561 tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact ggcttcagca 4621 gagcgcagat accaaatact gtccttctag tgtagccgta gttaggccac cacttcaaga 4681 actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg gctgctgcca 4741 gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg gataaggcgc 4801 agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga acgacctaca 4861 ccgaactgag atacctacag cgtgagctat gagaaagcgc cacgcttccc gaagggagaa 4921 aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg agggagcttc 4981 cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc 5041 gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg 5101 cctttttacg gttcctggcc ttttgctggc cttttgctca catgttcttt cctgcgttat 5161 cccctgattc tgtggataac cgtattaccg cctttgagtg agctgatacc gctcgccgca 5221 gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc ggaagagcgc ccaatacgca 5281 aaccgcctct ccccgcgcgt tggccgattc attaatgcag ctggcacgac aggtttcccg 5341 actggaaagc gggcagtgag cgcaacgcaa ttaatgtgag ttagctcact cattaggcac 5401 cccaggcttt acactttatg cttccggctc gtatgttgtg tggaattgtg agcggataac 5461 aattt //