LOCUS       B630pUbiHa-LGFP         4464 bp    DNA     circular SYN 08-FEB-2016
DEFINITION  Ligation of B629p35s-LGFP* into pUbiH-Nos*
ACCESSION   B630pUbiHa-LGFP
KEYWORDS    .
SOURCE      Unknown.
  ORGANISM  Unknown
            Unclassified.
REFERENCE   1  (bases 1 to 4464)
  AUTHORS   Self
  JOURNAL   Unpublished.
COMMENT     SECID/File created by Clone Manager, Scientific & Educational Software
COMMENT     SECNOTES|Vector molecule:  pUbiH-Nos*
               Fragment ends: KpnI and BamHI
               Fragment size: 3224
            Insert molecule:  B629p35s-LGFP*
               Fragment ends: BamHI and KpnI
               Fragment size: 1240
            Sequencing primers	
            M13RY	CACACAGGAAACAGCTATGAC
            pUCF	GCAAGGCGATTAAGTTGGGTA
FEATURES             Location/Qualifiers
     misc_feature    129..464
                     /gene="UbiHa"
                     /product="Promotor Helianthus anuus"
                     /SECDrawAs="Region"
                     /SECStyleId=1
     misc_feature    513..563
                     /gene="link"
                     /product="linker"
                     /SECDrawAs="Region"
                     /SECStyleId=1
     CDS             564..942
                     /gene="gfpm"
                     /product="GFPm"
                     /SECDrawAs="Gene"
                     /SECStyleId=1
     CDS             943..1131
                     /gene="int"
                     /product="STLS1"
                     /SECDrawAs="Gene"
                     /SECStyleId=1
     CDS             1132..1469
                     /gene="gfpm"
                     /product="GFPm"
                     /SECDrawAs="Gene"
                     /SECStyleId=1
     misc_feature    1479..1674
                     /gene="Tocs"
                     /product="Terminator"
                     /SECDrawAs="Region"
                     /SECStyleId=1
                     /SECName="Tocs"
                     /SECDescr="Terminator"
     CDS             2490..3350
                     /gene="Amp"
                     /product="ORF-1"
                     /SECDrawAs="Gene"
                     /SECStyleId=1
     misc_feature    3393..4109
                     /gene="ColE1"
                     /product="ColE1"
                     /SECDrawAs="Region"
                     /SECStyleId=1
ORIGIN      
        1 gtggaattgt gagcggataa caatttcaca caggaaacag ctatgaccat gattacgcca
       61 agctcgaaat taaccctcac taaagggaac aaaagctgga gctctctaga ggccatggcg
      121 gccgcgacaa aacttaagaa agtcaccaaa aagaaagtca aagacggcaa cggcaaccca
      181 gaagctacgt tctctttcta gattctatgc cctacctacc gcgtggtgca caacaacttg
      241 acccccatca ttgactctcc accgtacacg tgtcacgata ccatttgttg gtctcctacc
      301 acttacgtaa cattaatatt ccatccgtga ccgcctcttc ttcatcatcc tataaattca
      361 ccttcttatc tcttccgctt tcattcgttc atcatcatca caattaacaa caatccaaag
      421 caatcttctc tcatattctt atctcttcct gtttcgcttc gtctactagt ggatcccccg
      481 ggctgcagga attcaagctt acgcgttgta caggagtacc gccccgtccg gtcctgcccg
      541 tcaccgagat cagcggagag ttcatgggca agggcgagga actgttcact ggcgtggtcc
      601 caatcctggt ggaactggat ggtgatgtga acgggcacaa gttctccgtc agcggagagg
      661 gtgaaggtga tgccacctac ggaaagctca ccctgaagtt catctgcact accggaaagc
      721 tccctgttcc gtggccaacc ctcgtcacca ctttcaccta cggtgttcag tgcttctccc
      781 gttacccaga tcacatgaag cagcatgact tcttcaagag cgccatgccc gaaggctacg
      841 tgcaagaaag gactatcttc ttcaaggatg acgggaacta caagacacgt gccgaagtca
      901 agttcgaagg tgataccctg gtgaaccgca tcgagctgaa aggtaagttt ctgcttctac
      961 ctttgatata tatataataa ttatcattaa ttagtagtaa tataatattt caaatatttt
     1021 tttcaaaata aaagaatgta gtatatagca attgcttttc tgtagtttat aagtgtgtat
     1081 attttaattt ataacttttc taatatatga ccaaaatttg ttgatgtgca ggtatcgatt
     1141 tcaaggaaga tggaaacatc ctcggacaca agctggagta caactacaac tcccacaacg
     1201 tatacatcat ggccgacaag cagaagaacg gcatcaaggt gaacttcaag atcaggcaca
     1261 acatcgaaga tggaagcgtg caactggcgg accactacca gcagaacacg cccatcggcg
     1321 atggccctgt cctgctgccg gacaaccatt acctgtccac gcaatctgcc ctctccaagg
     1381 accccaacga gaagagggac cacatggtcc tgctggagtt cgtgacggct gctgggatca
     1441 cgcatggcat ggatgaactc tacaagtgac ccggtcgacc ctgctttaat gagatatgcg
     1501 agacgcctat gatcgcatga tatttgcttt caattctgtt gtgcacgttg taaaaaacct
     1561 gagcatgtgt agctcagatc cttaccgccg gtttcggttc attctaatga atatatcacc
     1621 cgttactatc gtatttttat gaataatatt ctccgttcaa tttactgatt gtcctcgaca
     1681 ggccttaagg gccagatctt gggcccggta cccaattcgc cctatagtga gtcgtattac
     1741 aattcactgg ccgtcgtttt acaacgtcgt gactgggaaa accctggcgt tacccaactt
     1801 aatcgccttg cagcacatcc ccctttcgcc agctggcgta atagcgaaga ggcccgcacc
     1861 gatcgccctt cccaacagtt gcgcagcctg aatggcgaat ggaaattgta agcgttaata
     1921 ttttgttaaa attcgcgtta aatttttgtt aaatcagctc attttttaac caataggccg
     1981 aaatcggcaa aatcccttat aaatcaaaag aatagaccga gatagggttg agtgttgttc
     2041 cagtttggaa caagagtcca ctattaaaga acgtggactc caacgtcaaa gggcgaaaaa
     2101 ccgtctatca gggcgatggc ccactacgtg aaccatcacc ctaatcaagt tttttggggt
     2161 cgaggtgccg taaagcacta aatcggaacc ctaaagggag cccccgattt agagcttgac
     2221 ggggaaagcc ggcgaacgtg gcgagaaagg aagggaagaa agcgaaagga gcgggcgcta
     2281 gggcgctggc aagtgtagcg gtcacgctgc gcgtaaccac cacacccgcc gcgcttaatg
     2341 cgccgctaca gggcgcgtca ggtggcactt ttcggggaaa tgtgcgcgga acccctattt
     2401 gtttattttt ctaaatacat tcaaatatgt atccgctcat gagacaataa ccctgataaa
     2461 tgcttcaata atattgaaaa aggaagagta tgagtattca acatttccgt gtcgccctta
     2521 ttcccttttt tgcggcattt tgccttcctg tttttgctca cccagaaacg ctggtgaaag
     2581 taaaagatgc tgaagatcag ttgggtgcac gagtgggtta catcgaactg gatctcaaca
     2641 gcggtaagat ccttgagagt tttcgccccg aagaacgttt tccaatgatg agcactttta
     2701 aagttctgct atgtggcgcg gtattatccc gtattgacgc cgggcaagag caactcggtc
     2761 gccgcataca ctattctcag aatgacttgg ttgagtactc accagtcaca gaaaagcatc
     2821 ttacggatgg catgacagta agagaattat gcagtgctgc cataaccatg agtgataaca
     2881 ctgcggccaa cttacttctg acaacgatcg gaggaccgaa ggagctaacc gcttttttgc
     2941 acaacatggg ggatcatgta actcgccttg atcgttggga accggagctg aatgaagcca
     3001 taccaaacga cgagcgtgac accacgatgc ctgtagcaat ggcaacaacg ttgcgcaaac
     3061 tattaactgg cgaactactt actctagctt cccggcaaca attaatagac tggatggagg
     3121 cggataaagt tgcaggacca cttctgcgct cggcccttcc ggctggctgg tttattgctg
     3181 ataaatctgg agccggtgag cgtgggtctc gcggtatcat tgcagcactg gggccagatg
     3241 gtaagccctc ccgtatcgta gttatctaca cgacggggag tcaggcaact atggatgaac
     3301 gaaatagaca gatcgctgag ataggtgcct cactgattaa gcattggtaa ctgtcagacc
     3361 aagtttactc atatatactt tagattgatt taaaacttca tttttaattt aaaaggatct
     3421 aggtgaagat cctttttgat aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc
     3481 actgagcgtc agaccccgta gaaaagatca aaggatcttc ttgagatcct ttttttctgc
     3541 gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt tgtttgccgg
     3601 atcaagagct accaactctt tttccgaagg taactggctt cagcagagcg cagataccaa
     3661 atactgtcct tctagtgtag ccgtagttag gccaccactt caagaactct gtagcaccgc
     3721 ctacatacct cgctctgcta atcctgttac cagtggctgc tgccagtggc gataagtcgt
     3781 gtcttaccgg gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa
     3841 cggggggttc gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc
     3901 tacagcgtga gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc
     3961 cggtaagcgg cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct
     4021 ggtatcttta tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat
     4081 gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc
     4141 tggccttttg ctggcctttt gctcacatgt tctttcctgc gttatcccct gattctgtgg
     4201 ataaccgtat taccgccttt gagtgagctg ataccgctcg ccgcagccga acgaccgagc
     4261 gcagcgagtc agtgagcgag gaagcggaag agcgcccaat acgcaaaccg cctctccccg
     4321 cgcgttggcc gattcattaa tgcagctggc acgacaggtt tcccgactgg aaagcgggca
     4381 gtgagcgcaa cgcaattaat gtgagttagc tcactcatta ggcaccccag gctttacact
     4441 ttatgcttcc ggctcgtatg ttgt
//
