LOCUS       B631pUbiAt-LGFP         5601 bp    DNA     circular SYN 08-FEB-2016
DEFINITION  Ligation of B629p35s-LGFP* into 204pUbiAtmu-ocs*
ACCESSION   B631pUbiAt-LGFP
KEYWORDS    .
SOURCE      Unknown.
  ORGANISM  Unknown
            Unclassified.
REFERENCE   1  (bases 1 to 5601)
  AUTHORS   Self
  JOURNAL   Unpublished.
COMMENT     SECID/File created by Clone Manager, Scientific & Educational Software
COMMENT     SECNOTES|Vector molecule:  204pUbiAtmu-ocs*
               Fragment ends: KpnI and BamHI
               Fragment size: 4361
            Insert molecule:  B629p35s-LGFP*
               Fragment ends: BamHI and KpnI
               Fragment size: 1240
            Sequencing primers	
            UbiAT-F3	GCTATTGCTTCACCGCCTTA
            pUCF	GCAAGGCGATTAAGTTGGGTA
            
FEATURES             Location/Qualifiers
     misc_feature    126..1601
                     /gene="UBQ10"
                     /SECDrawAs="Region"
                     /SECStyleId=1
     misc_feature    1230..1293
                     /product="Ubi cds 5"
                     /SECDrawAs="Region"
                     /SECStyleId=1
     misc_feature    1296..1599
                     /gene="int"
                     /SECDrawAs="Region"
                     /SECStyleId=1
     misc_feature    1650..1700
                     /gene="link"
                     /product="linker"
                     /SECDrawAs="Region"
                     /SECStyleId=1
     CDS             1701..2079
                     /gene="gfpm"
                     /product="GFPm"
                     /SECDrawAs="Gene"
                     /SECStyleId=1
     CDS             2080..2268
                     /gene="int"
                     /product="STLS1"
                     /SECDrawAs="Gene"
                     /SECStyleId=1
     CDS             2269..2606
                     /gene="gfpm"
                     /product="GFPm"
                     /SECDrawAs="Gene"
                     /SECStyleId=1
     misc_feature    2616..2811
                     /gene="Tocs"
                     /product="Terminator"
                     /SECDrawAs="Region"
                     /SECStyleId=1
                     /SECName="Tocs"
                     /SECDescr="Terminator"
     misc_feature    3627..4487
                     /gene="Amp"
                     /product="ORF-1"
                     /SECDrawAs="Gene"
                     /SECStyleId=1
                     /SECName="Amp"
                     /SECDescr="ORF-1"
     misc_feature    4530..5246
                     /gene="ColE1"
                     /product="ColE1"
                     /SECDrawAs="Region"
                     /SECStyleId=1
                     /SECName="ColE1"
                     /SECDescr="ColE1"
ORIGIN      
        1 gtggaattgt gagcggataa caatttcaca caggaaacag ctatgaccat gattacgcca
       61 agctcgaaat taaccctcac taaagggaac aaaagctgga gctctctaga ggccatggcg
      121 gccgctttcc ttacattctg agcctctttc cttctaatcc actcatctgc atcttcttgt
      181 gtccttacta atacctcatt ggttccaaat tccctccctt taagcaccag ctcgtttctg
      241 ttcttccaca gcctcccaag tatccaaggg actaaagcct ccacattctt cagatcagga
      301 tattcttgtt taagatgttg aactctatgg aggtttgtat gaactgatga tctaggaccg
      361 gataagttcc cttcttcata gcgaacttat tcaaagaatg ttttgtgtat cattcttgtt
      421 acattgttat taatgaaaaa atattattgg tcattggact gaacacgagt gttaaatatg
      481 gaccaggccc caaataagat ccattgatat atgaattaaa taacaagaat aaatcgagtc
      541 accaaaccac ttgccttttt taacgagact tgttcaccaa cttgatacaa aagtcattat
      601 cctatgcaaa tcaataatca tacaaaaata tccaataaca ctaaaaaatt aaaagaaatg
      661 gataatttca caatatgtta tacgataaag aagttacttt tccaagaaat tcactgattt
      721 tataagccca cttgcattag ataaatggca aaaaaaaaca aaaaggaaaa gaaataaagc
      781 acgaagaatt ctagaaaata cgaaatacgc ttcaatgcag tgggacccac ggttcaatta
      841 ttgccaattt tcagctccac cgtatattta aaaaataaaa cgataatgct aaaaaaatat
      901 aaatcgtaac gatcgttaaa tctcaacggc tggatcttat gacgaccgtt agaaattgtg
      961 gttgtcgacg agtcagtaat aaacggcgtc aaagtggttg cagccggcac acacgagtcg
     1021 tgtttatcaa ctcaaagcac aaatactttt cctcaaccta aaaataaggc aattagccaa
     1081 aaacaacttt gcgtgtaaac aacgctcaat acacgtgtca ttttattatt agctattgct
     1141 tcaccgcctt agctttctcg tgacctagtc gtcctcgtct tttcttcttc ttcttctata
     1201 aaacaatacc caaagagctc ttcttcttca caattcagat ttcaatttct caaaatctta
     1261 aaaactttct ctcaattctc tctaccgtga tcaaggtaaa tttctgtgtt ccttattctc
     1321 tcaaaatctt cgattttgtt ttcgttcgat cccaatttcg tatatgttct ttggtttaga
     1381 ttctgttaat cttagatcga agacgatttt ctgggtttga tcgttagata tcatcttaat
     1441 tctcgattag ggtttcatag atatcatccg atttgttcaa ataatttgag ttttgtcgaa
     1501 taattactct tcgatttgtg atttctatct agatctggtg ttagtttcta gtttgtgcga
     1561 tcgaatttgt cgattaatct gagtttttct gattaacagg tactagtgga tcccccgggc
     1621 tgcaggaatt caagcttacg cgttgtacag gagtaccgcc ccgtccggtc ctgcccgtca
     1681 ccgagatcag cggagagttc atgggcaagg gcgaggaact gttcactggc gtggtcccaa
     1741 tcctggtgga actggatggt gatgtgaacg ggcacaagtt ctccgtcagc ggagagggtg
     1801 aaggtgatgc cacctacgga aagctcaccc tgaagttcat ctgcactacc ggaaagctcc
     1861 ctgttccgtg gccaaccctc gtcaccactt tcacctacgg tgttcagtgc ttctcccgtt
     1921 acccagatca catgaagcag catgacttct tcaagagcgc catgcccgaa ggctacgtgc
     1981 aagaaaggac tatcttcttc aaggatgacg ggaactacaa gacacgtgcc gaagtcaagt
     2041 tcgaaggtga taccctggtg aaccgcatcg agctgaaagg taagtttctg cttctacctt
     2101 tgatatatat ataataatta tcattaatta gtagtaatat aatatttcaa atattttttt
     2161 caaaataaaa gaatgtagta tatagcaatt gcttttctgt agtttataag tgtgtatatt
     2221 ttaatttata acttttctaa tatatgacca aaatttgttg atgtgcaggt atcgatttca
     2281 aggaagatgg aaacatcctc ggacacaagc tggagtacaa ctacaactcc cacaacgtat
     2341 acatcatggc cgacaagcag aagaacggca tcaaggtgaa cttcaagatc aggcacaaca
     2401 tcgaagatgg aagcgtgcaa ctggcggacc actaccagca gaacacgccc atcggcgatg
     2461 gccctgtcct gctgccggac aaccattacc tgtccacgca atctgccctc tccaaggacc
     2521 ccaacgagaa gagggaccac atggtcctgc tggagttcgt gacggctgct gggatcacgc
     2581 atggcatgga tgaactctac aagtgacccg gtcgaccctg ctttaatgag atatgcgaga
     2641 cgcctatgat cgcatgatat ttgctttcaa ttctgttgtg cacgttgtaa aaaacctgag
     2701 catgtgtagc tcagatcctt accgccggtt tcggttcatt ctaatgaata tatcacccgt
     2761 tactatcgta tttttatgaa taatattctc cgttcaattt actgattgtc ctcgacaggc
     2821 cttaagggcc agatcttggg cccggtaccc aattcgccct atagtgagtc gtattacaat
     2881 tcactggccg tcgttttaca acgtcgtgac tgggaaaacc ctggcgttac ccaacttaat
     2941 cgccttgcag cacatccccc tttcgccagc tggcgtaata gcgaagaggc ccgcaccgat
     3001 cgcccttccc aacagttgcg cagcctgaat ggcgaatgga aattgtaagc gttaatattt
     3061 tgttaaaatt cgcgttaaat ttttgttaaa tcagctcatt ttttaaccaa taggccgaaa
     3121 tcggcaaaat cccttataaa tcaaaagaat agaccgagat agggttgagt gttgttccag
     3181 tttggaacaa gagtccacta ttaaagaacg tggactccaa cgtcaaaggg cgaaaaaccg
     3241 tctatcaggg cgatggccca ctacgtgaac catcacccta atcaagtttt ttggggtcga
     3301 ggtgccgtaa agcactaaat cggaacccta aagggagccc ccgatttaga gcttgacggg
     3361 gaaagccggc gaacgtggcg agaaaggaag ggaagaaagc gaaaggagcg ggcgctaggg
     3421 cgctggcaag tgtagcggtc acgctgcgcg taaccaccac acccgccgcg cttaatgcgc
     3481 cgctacaggg cgcgtcaggt ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt
     3541 tatttttcta aatacattca aatatgtatc cgctcatgag acaataaccc tgataaatgc
     3601 ttcaataata ttgaaaaagg aagagtatga gtattcaaca tttccgtgtc gcccttattc
     3661 ccttttttgc ggcattttgc cttcctgttt ttgctcaccc agaaacgctg gtgaaagtaa
     3721 aagatgctga agatcagttg ggtgcacgag tgggttacat cgaactggat ctcaacagcg
     3781 gtaagatcct tgagagtttt cgccccgaag aacgttttcc aatgatgagc acttttaaag
     3841 ttctgctatg tggcgcggta ttatcccgta ttgacgccgg gcaagagcaa ctcggtcgcc
     3901 gcatacacta ttctcagaat gacttggttg agtactcacc agtcacagaa aagcatctta
     3961 cggatggcat gacagtaaga gaattatgca gtgctgccat aaccatgagt gataacactg
     4021 cggccaactt acttctgaca acgatcggag gaccgaagga gctaaccgct tttttgcaca
     4081 acatggggga tcatgtaact cgccttgatc gttgggaacc ggagctgaat gaagccatac
     4141 caaacgacga gcgtgacacc acgatgcctg tagcaatggc aacaacgttg cgcaaactat
     4201 taactggcga actacttact ctagcttccc ggcaacaatt aatagactgg atggaggcgg
     4261 ataaagttgc aggaccactt ctgcgctcgg cccttccggc tggctggttt attgctgata
     4321 aatctggagc cggtgagcgt gggtctcgcg gtatcattgc agcactgggg ccagatggta
     4381 agccctcccg tatcgtagtt atctacacga cggggagtca ggcaactatg gatgaacgaa
     4441 atagacagat cgctgagata ggtgcctcac tgattaagca ttggtaactg tcagaccaag
     4501 tttactcata tatactttag attgatttaa aacttcattt ttaatttaaa aggatctagg
     4561 tgaagatcct ttttgataat ctcatgacca aaatccctta acgtgagttt tcgttccact
     4621 gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg agatcctttt tttctgcgcg
     4681 taatctgctg cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc
     4741 aagagctacc aactcttttt ccgaaggtaa ctggcttcag cagagcgcag ataccaaata
     4801 ctgtccttct agtgtagccg tagttaggcc accacttcaa gaactctgta gcaccgccta
     4861 catacctcgc tctgctaatc ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc
     4921 ttaccgggtt ggactcaaga cgatagttac cggataaggc gcagcggtcg ggctgaacgg
     4981 ggggttcgtg cacacagccc agcttggagc gaacgaccta caccgaactg agatacctac
     5041 agcgtgagct atgagaaagc gccacgcttc ccgaagggag aaaggcggac aggtatccgg
     5101 taagcggcag ggtcggaaca ggagagcgca cgagggagct tccaggggga aacgcctggt
     5161 atctttatag tcctgtcggg tttcgccacc tctgacttga gcgtcgattt ttgtgatgct
     5221 cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc ggccttttta cggttcctgg
     5281 ccttttgctg gccttttgct cacatgttct ttcctgcgtt atcccctgat tctgtggata
     5341 accgtattac cgcctttgag tgagctgata ccgctcgccg cagccgaacg accgagcgca
     5401 gcgagtcagt gagcgaggaa gcggaagagc gcccaatacg caaaccgcct ctccccgcgc
     5461 gttggccgat tcattaatgc agctggcacg acaggtttcc cgactggaaa gcgggcagtg
     5521 agcgcaacgc aattaatgtg agttagctca ctcattaggc accccaggct ttacacttta
     5581 tgcttccggc tcgtatgttg t
//
