LOCUS       B633pUbiZm-LGFP         5641 bp    DNA     circular SYN 08-FEB-2016
DEFINITION  Ligation of pLNU* into B636pLGFP-ocs*
ACCESSION   B633pUbiZm-LGFP
KEYWORDS    .
SOURCE      Unknown.
  ORGANISM  Unknown
            Unclassified.
REFERENCE   1  (bases 1 to 5641)
  AUTHORS   Self
  JOURNAL   Unpublished.
COMMENT     SECID/File created by Clone Manager, Scientific & Educational Software
COMMENT     SECNOTES|Vector molecule:  B636pLGFP-ocs*
               Fragment ends: BamHI and NotI
               Fragment size: 4112
            Insert molecule:  pLNU*
               Fragment ends: NotI and BamHI
               Fragment size: 1529
            Sequencing primers	
            M13RY	CACACAGGAAACAGCTATGAC
            GFP-seq	CCAGTTCCACCAGGATTG
FEATURES             Location/Qualifiers
     misc_feature    106..1614
                     /gene="UbiZm"
                     /product="Promotor"
                     /SECDrawAs="Region"
                     /SECStyleId=1
     misc_feature    1093..1612
                     /gene="int"
                     /product="Intron"
                     /SECDrawAs="Region"
                     /SECStyleId=1
     misc_feature    1664..1714
                     /gene="link"
                     /product="linker"
                     /SECDrawAs="Region"
                     /SECStyleId=1
     CDS             1715..2093
                     /gene="gfpm"
                     /product="GFPm"
                     /SECDrawAs="Gene"
                     /SECStyleId=1
     CDS             2094..2282
                     /gene="int"
                     /product="STLS1"
                     /SECDrawAs="Gene"
                     /SECStyleId=1
     CDS             2283..2620
                     /gene="gfpm"
                     /product="GFPm"
                     /SECDrawAs="Gene"
                     /SECStyleId=1
     misc_feature    2630..2825
                     /gene="Tocs"
                     /product="Terminator"
                     /SECDrawAs="Region"
                     /SECStyleId=1
                     /SECName="Tocs"
                     /SECDescr="Terminator"
     misc_feature    3641..4501
                     /gene="Amp"
                     /product="ORF-1"
                     /SECDrawAs="Gene"
                     /SECStyleId=1
                     /SECName="Amp"
                     /SECDescr="ORF-1"
     CDS             4544..5260
                     /gene="ColE1"
                     /product="ColE1"
                     /SECDrawAs="Region"
                     /SECStyleId=1
                     /SECName="ColE1"
                     /SECDescr="ColE1"
ORIGIN      
        1 cacacaggaa acagctatga ccatgattac gccaagctcg aaattaaccc tcactaaagg
       61 gaacaaaagc tggagctctc tagaggccat ggcggccgca ctaggctgca gtgcagcgtg
      121 acccggtcgt gcccctctct agagataatg agcattgcat gtctaagtta taaaaaatta
      181 ccacatattt tttttgtcac acttgtttga agtgcagttt atctatcttt atacatatat
      241 ttaaacttta ctctacgaat aatataatct atagtactac aataatatca gtgttttaga
      301 gaatcatata aatgaacagt tagacatggt ctaaaggaca attgagtatt ttgacaacag
      361 gactctacag ttttatcttt ttagtgtgca tgtgttctcc tttttttttg caaatagctt
      421 cacctatata atacttcatc cattttatta gtacatccat ttagggttta gggttaatgg
      481 tttttataga ctaatttttt tagtacatct attttattct attttagcct ctaaattaag
      541 aaaactaaaa ctctatttta gtttttttat ttaataattt agatataaaa tagaataaaa
      601 taaagtgact aaaaattaaa caaataccct ttaagaaatt aaaaaaacta aggaaacatt
      661 tttcttgttt cgagtagata atgccagcct gttaaacgcc gtcgatcgac gagtctaacg
      721 gacaccaacc agcgaaccag cagcgtcgcg tcgggccaag cgaagcagac ggcacggcat
      781 ctctgtcgct gcctctggac ccctctcgag agttccgctc caccgttgga cttgctccgc
      841 tgtcggcatc cagaaattgc gtggcggagc ggcagacgtg agccggcacg gcaggcggcc
      901 tcctcctcct ctcacggcac cggcagctac gggggattcc tttcccaccg ctccttcgct
      961 ttcccttcct cgcccgccgt aataaataga caccccctcc acaccctctt tccccaacct
     1021 cgtgttgttc ggagcgcaca cacacacaac cagatctccc ccaaatccac ccgtcggcac
     1081 ctccgcttca aggtacgccg ctcgtcctcc cccccccccc ctctctacct tctctagatc
     1141 ggcgttccgg tccatggtta gggcccggta gttctacttc tgttcatgtt tgtgttagat
     1201 ccgtgtttgt gttagatccg tgctgctagc gttcgtacac ggatgcgacc tgtacgtcag
     1261 acacgttctg attgctaact tgccagtgtt tctctttggg gaatcctggg atggctctag
     1321 ccgttccgca gacgggatcg atctaggata ggtatacatg ttgatgtggg ttttactgat
     1381 gcatatacat gatggcatat gcagcatcta ttcatatgct ctaaccttga gtacctatct
     1441 attataataa acaagtatgt tttataatta ttttgatctt gatatacttg gatgatggca
     1501 tatgcagcag ctatatgtgg atttttttag ccctgccttc atacgctatt tatttgcttg
     1561 gtactgtttc ttttgtcgat gctcaccctg ttgtttggtg ttacttctgc aggtactagt
     1621 tggatccccc gggctgcagg aattcaagct tacgcgttgt acaggagtac cgccccgtcc
     1681 ggtcctgccc gtcaccgaga tcagcggaga gttcatgggc aagggcgagg aactgttcac
     1741 tggcgtggtc ccaatcctgg tggaactgga tggtgatgtg aacgggcaca agttctccgt
     1801 cagcggagag ggtgaaggtg atgccaccta cggaaagctc accctgaagt tcatctgcac
     1861 taccggaaag ctccctgttc cgtggccaac cctcgtcacc actttcacct acggtgttca
     1921 gtgcttctcc cgttacccag atcacatgaa gcagcatgac ttcttcaaga gcgccatgcc
     1981 cgaaggctac gtgcaagaaa ggactatctt cttcaaggat gacgggaact acaagacacg
     2041 tgccgaagtc aagttcgaag gtgataccct ggtgaaccgc atcgagctga aaggtaagtt
     2101 tctgcttcta cctttgatat atatataata attatcatta attagtagta atataatatt
     2161 tcaaatattt ttttcaaaat aaaagaatgt agtatatagc aattgctttt ctgtagttta
     2221 taagtgtgta tattttaatt tataactttt ctaatatatg accaaaattt gttgatgtgc
     2281 aggtatcgat ttcaaggaag atggaaacat cctcggacac aagctggagt acaactacaa
     2341 ctcccacaac gtatacatca tggccgacaa gcagaagaac ggcatcaagg tgaacttcaa
     2401 gatcaggcac aacatcgaag atggaagcgt gcaactggcg gaccactacc agcagaacac
     2461 gcccatcggc gatggccctg tcctgctgcc ggacaaccat tacctgtcca cgcaatctgc
     2521 cctctccaag gaccccaacg agaagaggga ccacatggtc ctgctggagt tcgtgacggc
     2581 tgctgggatc acgcatggca tggatgaact ctacaagtga cccggtcgac cctgctttaa
     2641 tgagatatgc gagacgccta tgatcgcatg atatttgctt tcaattctgt tgtgcacgtt
     2701 gtaaaaaacc tgagcatgtg tagctcagat ccttaccgcc ggtttcggtt cattctaatg
     2761 aatatatcac ccgttactat cgtattttta tgaataatat tctccgttca atttactgat
     2821 tgtcctcgac aggccttaag ggccagatct tgggcccggt acccaattcg ccctatagtg
     2881 agtcgtatta caattcactg gccgtcgttt tacaacgtcg tgactgggaa aaccctggcg
     2941 ttacccaact taatcgcctt gcagcacatc cccctttcgc cagctggcgt aatagcgaag
     3001 aggcccgcac cgatcgccct tcccaacagt tgcgcagcct gaatggcgaa tggaaattgt
     3061 aagcgttaat attttgttaa aattcgcgtt aaatttttgt taaatcagct cattttttaa
     3121 ccaataggcc gaaatcggca aaatccctta taaatcaaaa gaatagaccg agatagggtt
     3181 gagtgttgtt ccagtttgga acaagagtcc actattaaag aacgtggact ccaacgtcaa
     3241 agggcgaaaa accgtctatc agggcgatgg cccactacgt gaaccatcac cctaatcaag
     3301 ttttttgggg tcgaggtgcc gtaaagcact aaatcggaac cctaaaggga gcccccgatt
     3361 tagagcttga cggggaaagc cggcgaacgt ggcgagaaag gaagggaaga aagcgaaagg
     3421 agcgggcgct agggcgctgg caagtgtagc ggtcacgctg cgcgtaacca ccacacccgc
     3481 cgcgcttaat gcgccgctac agggcgcgtc aggtggcact tttcggggaa atgtgcgcgg
     3541 aacccctatt tgtttatttt tctaaataca ttcaaatatg tatccgctca tgagacaata
     3601 accctgataa atgcttcaat aatattgaaa aaggaagagt atgagtattc aacatttccg
     3661 tgtcgccctt attccctttt ttgcggcatt ttgccttcct gtttttgctc acccagaaac
     3721 gctggtgaaa gtaaaagatg ctgaagatca gttgggtgca cgagtgggtt acatcgaact
     3781 ggatctcaac agcggtaaga tccttgagag ttttcgcccc gaagaacgtt ttccaatgat
     3841 gagcactttt aaagttctgc tatgtggcgc ggtattatcc cgtattgacg ccgggcaaga
     3901 gcaactcggt cgccgcatac actattctca gaatgacttg gttgagtact caccagtcac
     3961 agaaaagcat cttacggatg gcatgacagt aagagaatta tgcagtgctg ccataaccat
     4021 gagtgataac actgcggcca acttacttct gacaacgatc ggaggaccga aggagctaac
     4081 cgcttttttg cacaacatgg gggatcatgt aactcgcctt gatcgttggg aaccggagct
     4141 gaatgaagcc ataccaaacg acgagcgtga caccacgatg cctgtagcaa tggcaacaac
     4201 gttgcgcaaa ctattaactg gcgaactact tactctagct tcccggcaac aattaataga
     4261 ctggatggag gcggataaag ttgcaggacc acttctgcgc tcggcccttc cggctggctg
     4321 gtttattgct gataaatctg gagccggtga gcgtgggtct cgcggtatca ttgcagcact
     4381 ggggccagat ggtaagccct cccgtatcgt agttatctac acgacgggga gtcaggcaac
     4441 tatggatgaa cgaaatagac agatcgctga gataggtgcc tcactgatta agcattggta
     4501 actgtcagac caagtttact catatatact ttagattgat ttaaaacttc atttttaatt
     4561 taaaaggatc taggtgaaga tcctttttga taatctcatg accaaaatcc cttaacgtga
     4621 gttttcgttc cactgagcgt cagaccccgt agaaaagatc aaaggatctt cttgagatcc
     4681 tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt
     4741 ttgtttgccg gatcaagagc taccaactct ttttccgaag gtaactggct tcagcagagc
     4801 gcagatacca aatactgtcc ttctagtgta gccgtagtta ggccaccact tcaagaactc
     4861 tgtagcaccg cctacatacc tcgctctgct aatcctgtta ccagtggctg ctgccagtgg
     4921 cgataagtcg tgtcttaccg ggttggactc aagacgatag ttaccggata aggcgcagcg
     4981 gtcgggctga acggggggtt cgtgcacaca gcccagcttg gagcgaacga cctacaccga
     5041 actgagatac ctacagcgtg agctatgaga aagcgccacg cttcccgaag ggagaaaggc
     5101 ggacaggtat ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg agcttccagg
     5161 gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg
     5221 atttttgtga tgctcgtcag gggggcggag cctatggaaa aacgccagca acgcggcctt
     5281 tttacggttc ctggcctttt gctggccttt tgctcacatg ttctttcctg cgttatcccc
     5341 tgattctgtg gataaccgta ttaccgcctt tgagtgagct gataccgctc gccgcagccg
     5401 aacgaccgag cgcagcgagt cagtgagcga ggaagcggaa gagcgcccaa tacgcaaacc
     5461 gcctctcccc gcgcgttggc cgattcatta atgcagctgg cacgacaggt ttcccgactg
     5521 gaaagcgggc agtgagcgca acgcaattaa tgtgagttag ctcactcatt aggcacccca
     5581 ggctttacac tttatgcttc cggctcgtat gttgtgtgga attgtgagcg gataacaatt
     5641 t
//
