LOCUS       B635pActOs-LGFP         5526 bp    DNA     circular SYN 08-FEB-2016
DEFINITION  Ligation of B629p35s-LGFP* into B627pAct-ocs*
ACCESSION   B635pActOs-LGFP
KEYWORDS    .
SOURCE      Unknown.
  ORGANISM  Unknown
            Unclassified.
REFERENCE   1  (bases 1 to 5526)
  AUTHORS   Self
  JOURNAL   Unpublished.
COMMENT     SECID/File created by Clone Manager, Scientific & Educational Software
COMMENT     SECNOTES|Vector molecule:  B627pAct-ocs*
               Fragment ends: KpnI and XmaI
               Fragment size: 4292
            Insert molecule:  B629p35s-LGFP*
               Fragment ends: XmaI and KpnI
               Fragment size: 1234
            Sequencing primers	
            F3F	CAGCATTGTTCATCGGTAGT
            pUCF	GCAAGGCGATTAAGTTGGGTA
FEATURES             Location/Qualifiers
     misc_feature    103..1500
                     /gene="ACT"
                     /product="Actin Promotor"
                     /SECDrawAs="Region"
                     /SECStyleId=1
     CDS             946..1500
                     /gene="mRNA"
                     /SECDrawAs="Gene"
                     /SECStyleId=1
     misc_feature    1036..1497
                     /gene="int"
                     /SECDrawAs="Region"
                     /SECStyleId=1
     misc_feature    1549..1599
                     /gene="link"
                     /product="linker"
                     /SECDrawAs="Region"
                     /SECStyleId=1
     CDS             1600..1978
                     /gene="gfpm"
                     /product="GFPm"
                     /SECDrawAs="Gene"
                     /SECStyleId=1
     CDS             1979..2167
                     /gene="int"
                     /product="STLS1"
                     /SECDrawAs="Gene"
                     /SECStyleId=1
     CDS             2168..2505
                     /gene="gfpm"
                     /product="GFPm"
                     /SECDrawAs="Gene"
                     /SECStyleId=1
     misc_feature    2515..2710
                     /gene="OCS"
                     /product="Terminator"
                     /SECDrawAs="Region"
                     /SECStyleId=1
                     /SECName="OCS"
                     /SECDescr="Terminator"
     misc_feature    3526..4386
                     /gene="Amp"
                     /product="ORF-1"
                     /SECDrawAs="Gene"
                     /SECStyleId=1
                     /SECName="Amp"
                     /SECDescr="ORF-1"
     misc_feature    4429..5145
                     /gene="ColE1"
                     /product="ColE1"
                     /SECDrawAs="Region"
                     /SECStyleId=1
                     /SECName="ColE1"
                     /SECDescr="ColE1"
ORIGIN      
        1 cacacaggaa acagctatga ccatgattac gccaagctcg aaattaaccc tcactaaagg
       61 gaacaaaagc tggagctctc tagaggccat ggcggccgca ggtcgaggtc attcatatgc
      121 ttgagaagag agtcgggata gtccaaaata aaacaaaggt aagattacct ggtcaaaagt
      181 gaaaacatca gttaaaaggt ggtataaagt aaaatatcgg taataaaagg tggcccaaag
      241 tgaaatttac tcttttctac tattataaaa attgaggatg tttttgtcgg tactttgata
      301 cgtcattttt gtatgaattg gtttttaagt ttattcgctt ttggaaatgc atatctgtat
      361 ttgagtcggg ttttaagttc gtttgctttt gtaaatacag agggatttgt ataagaaata
      421 tctttaaaaa aacccatatg ctaatttgac ataatttttg agaaaaatat atattcaggc
      481 gaattctcac aatgaacaat aataagatta aaatagcttt cccccgttgc agcgcatggg
      541 tattttttct agtaaaaata aaagataaac ttagactcaa aacatttaca aaaacaaccc
      601 ctaaagttcc taaagcccaa agtgctatcc acgatccata gcaagcccag cccaacccaa
      661 cccaacccaa cccaccccag tccagccaac tggacaatag tctccacacc cccccactat
      721 caccgtgagt tgtccgcacg caccgcacgt ctcgcagcca aaaaaaaaaa aagaaagaaa
      781 aaaaagaaaa agaaaaaaca gcaggtgggt ccgggtcgtg ggggccggaa acgcgaggag
      841 gatcgcgagc cagcgacgag gccggccctc cctccgcttc caaagaaacg ccccccatcg
      901 ccactatata catacccccc cctctcctcc catcccccca accctaccac caccaccacc
      961 accacctcca cctcctcccc cctcgctgcc ggacgacgag ctcctccccc ctccccctcc
     1021 gccgccgccg cgccggtaac caccccgccc ctctcctctt tctttctccg tttttttttt
     1081 ccgtctcggt ctcgatcttt ggccttggta gtttgggtgg gcgagaggcg gcttcgtgcg
     1141 cgcccagatc ggtgcgcggg aggggcggga tctcgcggct ggggctctcg ccggcgtgga
     1201 tccggcccgg atctcgcggg gaatggggct ctcggatgta gatctgcgat ccgccgttgt
     1261 tgggggagat gatggggggt ttaaaatttc cgccatgcta aacaagatca ggaagagggg
     1321 aaaagggcac tatggtttat atttttatat atttctgctg cttcgtcagg cttagatgtg
     1381 ctagatcttt ctttcttctt tttgtgggta gaatttgaat ccctcagcat tgttcatcgg
     1441 tagtttttct tttcatgatt tgtgacaaat gcagcctcgt gcggagcttt tttgtaggta
     1501 ctagttggat cccccgggct gcaggaattc aagcttacgc gttgtacagg agtaccgccc
     1561 cgtccggtcc tgcccgtcac cgagatcagc ggagagttca tgggcaaggg cgaggaactg
     1621 ttcactggcg tggtcccaat cctggtggaa ctggatggtg atgtgaacgg gcacaagttc
     1681 tccgtcagcg gagagggtga aggtgatgcc acctacggaa agctcaccct gaagttcatc
     1741 tgcactaccg gaaagctccc tgttccgtgg ccaaccctcg tcaccacttt cacctacggt
     1801 gttcagtgct tctcccgtta cccagatcac atgaagcagc atgacttctt caagagcgcc
     1861 atgcccgaag gctacgtgca agaaaggact atcttcttca aggatgacgg gaactacaag
     1921 acacgtgccg aagtcaagtt cgaaggtgat accctggtga accgcatcga gctgaaaggt
     1981 aagtttctgc ttctaccttt gatatatata taataattat cattaattag tagtaatata
     2041 atatttcaaa tatttttttc aaaataaaag aatgtagtat atagcaattg cttttctgta
     2101 gtttataagt gtgtatattt taatttataa cttttctaat atatgaccaa aatttgttga
     2161 tgtgcaggta tcgatttcaa ggaagatgga aacatcctcg gacacaagct ggagtacaac
     2221 tacaactccc acaacgtata catcatggcc gacaagcaga agaacggcat caaggtgaac
     2281 ttcaagatca ggcacaacat cgaagatgga agcgtgcaac tggcggacca ctaccagcag
     2341 aacacgccca tcggcgatgg ccctgtcctg ctgccggaca accattacct gtccacgcaa
     2401 tctgccctct ccaaggaccc caacgagaag agggaccaca tggtcctgct ggagttcgtg
     2461 acggctgctg ggatcacgca tggcatggat gaactctaca agtgacccgg tcgaccctgc
     2521 tttaatgaga tatgcgagac gcctatgatc gcatgatatt tgctttcaat tctgttgtgc
     2581 acgttgtaaa aaacctgagc atgtgtagct cagatcctta ccgccggttt cggttcattc
     2641 taatgaatat atcacccgtt actatcgtat ttttatgaat aatattctcc gttcaattta
     2701 ctgattgtcc tcgacaggcc ttaagggcca gatcttgggc ccggtaccca attcgcccta
     2761 tagtgagtcg tattacaatt cactggccgt cgttttacaa cgtcgtgact gggaaaaccc
     2821 tggcgttacc caacttaatc gccttgcagc acatccccct ttcgccagct ggcgtaatag
     2881 cgaagaggcc cgcaccgatc gcccttccca acagttgcgc agcctgaatg gcgaatggaa
     2941 attgtaagcg ttaatatttt gttaaaattc gcgttaaatt tttgttaaat cagctcattt
     3001 tttaaccaat aggccgaaat cggcaaaatc ccttataaat caaaagaata gaccgagata
     3061 gggttgagtg ttgttccagt ttggaacaag agtccactat taaagaacgt ggactccaac
     3121 gtcaaagggc gaaaaaccgt ctatcagggc gatggcccac tacgtgaacc atcaccctaa
     3181 tcaagttttt tggggtcgag gtgccgtaaa gcactaaatc ggaaccctaa agggagcccc
     3241 cgatttagag cttgacgggg aaagccggcg aacgtggcga gaaaggaagg gaagaaagcg
     3301 aaaggagcgg gcgctagggc gctggcaagt gtagcggtca cgctgcgcgt aaccaccaca
     3361 cccgccgcgc ttaatgcgcc gctacagggc gcgtcaggtg gcacttttcg gggaaatgtg
     3421 cgcggaaccc ctatttgttt atttttctaa atacattcaa atatgtatcc gctcatgaga
     3481 caataaccct gataaatgct tcaataatat tgaaaaagga agagtatgag tattcaacat
     3541 ttccgtgtcg cccttattcc cttttttgcg gcattttgcc ttcctgtttt tgctcaccca
     3601 gaaacgctgg tgaaagtaaa agatgctgaa gatcagttgg gtgcacgagt gggttacatc
     3661 gaactggatc tcaacagcgg taagatcctt gagagttttc gccccgaaga acgttttcca
     3721 atgatgagca cttttaaagt tctgctatgt ggcgcggtat tatcccgtat tgacgccggg
     3781 caagagcaac tcggtcgccg catacactat tctcagaatg acttggttga gtactcacca
     3841 gtcacagaaa agcatcttac ggatggcatg acagtaagag aattatgcag tgctgccata
     3901 accatgagtg ataacactgc ggccaactta cttctgacaa cgatcggagg accgaaggag
     3961 ctaaccgctt ttttgcacaa catgggggat catgtaactc gccttgatcg ttgggaaccg
     4021 gagctgaatg aagccatacc aaacgacgag cgtgacacca cgatgcctgt agcaatggca
     4081 acaacgttgc gcaaactatt aactggcgaa ctacttactc tagcttcccg gcaacaatta
     4141 atagactgga tggaggcgga taaagttgca ggaccacttc tgcgctcggc ccttccggct
     4201 ggctggttta ttgctgataa atctggagcc ggtgagcgtg ggtctcgcgg tatcattgca
     4261 gcactggggc cagatggtaa gccctcccgt atcgtagtta tctacacgac ggggagtcag
     4321 gcaactatgg atgaacgaaa tagacagatc gctgagatag gtgcctcact gattaagcat
     4381 tggtaactgt cagaccaagt ttactcatat atactttaga ttgatttaaa acttcatttt
     4441 taatttaaaa ggatctaggt gaagatcctt tttgataatc tcatgaccaa aatcccttaa
     4501 cgtgagtttt cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga
     4561 gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg
     4621 gtggtttgtt tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc
     4681 agagcgcaga taccaaatac tgtccttcta gtgtagccgt agttaggcca ccacttcaag
     4741 aactctgtag caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc
     4801 agtggcgata agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg
     4861 cagcggtcgg gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac
     4921 accgaactga gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga
     4981 aaggcggaca ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt
     5041 ccagggggaa acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag
     5101 cgtcgatttt tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg
     5161 gcctttttac ggttcctggc cttttgctgg ccttttgctc acatgttctt tcctgcgtta
     5221 tcccctgatt ctgtggataa ccgtattacc gcctttgagt gagctgatac cgctcgccgc
     5281 agccgaacga ccgagcgcag cgagtcagtg agcgaggaag cggaagagcg cccaatacgc
     5341 aaaccgcctc tccccgcgcg ttggccgatt cattaatgca gctggcacga caggtttccc
     5401 gactggaaag cgggcagtga gcgcaacgca attaatgtga gttagctcac tcattaggca
     5461 ccccaggctt tacactttat gcttccggct cgtatgttgt gtggaattgt gagcggataa
     5521 caattt
//
