LOCUS B443pmGFPM-nos 4150 bp DNA circular SYN 22-JAN-2016 DEFINITION Ligation of GFPmon-Hind-Sal* into pNos-BM* ACCESSION B443pmGFPM-nos KEYWORDS . SOURCE Unknown. ORGANISM Unknown Unclassified. REFERENCE 1 (bases 1 to 4150) AUTHORS Self JOURNAL Unpublished. COMMENT SECID/File created by Clone Manager, Scientific & Educational Software COMMENT SECNOTES|Vector molecule: pNos-BM* Fragment ends: SalI and HindIII Fragment size: 3218 Insert molecule: GFPmon-Hind-Sal* Fragment ends: HindIII and SalI Fragment size: 932 Primer for gene synthesis HindGFP cccgtcAAGCTTACGCGtcctccaccgaaATGGGCAAGGGC SalGFP CCCGTGgtcgaCGAGTCACTTGTAGAG Sequencing primers NosU CAAGACCGGCAACAGGATTC FEATURES Location/Qualifiers CDS 179..557 /gene="gfp" /product="GFP" /SECDrawAs="Gene" /SECStyleId=1 CDS 558..746 /gene="int" /product="STLS1" /SECDrawAs="Gene" /SECStyleId=1 CDS 747..1084 /gene="gfp" /product="GFP" /SECDrawAs="Gene" /SECStyleId=1 misc_feature 1106..1359 /gene="Tnos" /product="NOS-T" /SECDrawAs="Region" /SECStyleId=1 /SECName="Tnos" /SECDescr="NOS-T" misc_feature 2176..3036 /gene="Amp" /product="ORF-1" /SECDrawAs="Gene" /SECStyleId=1 /SECName="Amp" /SECDescr="ORF-1" CDS 3079..3795 /gene="ColE1" /product="ColE1" /SECDrawAs="Region" /SECStyleId=1 /SECName="ColE1" /SECDescr="ColE1" ORIGIN 1 gtggaattgt gagcggataa caatttcaca caggaaacag ctatgaccat gattacgcca 61 agctcgaaat taaccctcac taaagggaac aaaagctgga gctctctaga ggccatggcg 121 gccgcactag tggatccccc gggctgcagg aattcaagct tacgcgtcct ccaccgaaat 181 gggcaagggc gaggaactgt tcactggcgt ggtcccaatc ctggtggaac tggatggtga 241 tgtgaacggg cacaagttct ccgtcagcgg agagggtgaa ggtgatgcca cctacggaaa 301 gctcaccctg aagttcatct gcactaccgg aaagctccct gttccgtggc caaccctcgt 361 caccactttc acctacggtg ttcagtgctt ctcccggtac ccagatcaca tgaagcagca 421 tgacttcttc aagagcgcca tgcccgaagg ctacgtgcaa gaaaggacta tcttcttcaa 481 ggatgacggg aactacaaga cacgtgccga agtcaagttc gaaggtgata ccctggtgaa 541 ccgcatcgag ctgaaaggta agtttctgct tctacctttg atatatatat aataattatc 601 attaattagt agtaatataa tatttcaaat atttttttca aaataaaaga atgtagtata 661 tagcaattgc ttttctgtag tttataagtg tgtatatttt aatttataac ttttctaata 721 tatgaccaaa atttgttgat gtgcaggtat cgatttcaag gaagatggaa acatcctcgg 781 acacaagctg gagtacaact acaactccca caacgtatac atcatggccg acaagcagaa 841 gaacggcatc aaggtgaact tcaagatcag gcacaacatc gaagatggaa gcgtgcaact 901 ggcggaccac taccagcaga acacgcccat cggcgatggc cctgtcctgc tgccggacaa 961 ccattacctg tccacgcaat ctgccctctc caaggacccc aacgagaaga gggaccacat 1021 ggtcctgctg gagttcgtga cggctgctgg gatcacgcat ggcatggatg aactctacaa 1081 gtgactcgtc gactcgaatt tccccgatcg ttcaaacatt tggcaataaa gtttcttaag 1141 attgaatcct gttgccggtc ttgcgatgat tatcatataa tttctgttga attacgttaa 1201 gcatgtaata attaacatgt aatgcatgac gttatttatg agatgggttt ttatgattag 1261 agtcccgcaa ttatacattt aatacgcgat agaaaacaaa atatagcgcg caaactagga 1321 taaattatcg cgcgcggtgt catctatgtt actagatcgc tcgacaggcc ttaagggcca 1381 gatcttgggc ccggtaccca attcgcccta tagtgagtcg tattacaatt cactggccgt 1441 cgttttacaa cgtcgtgact gggaaaaccc tggcgttacc caacttaatc gccttgcagc 1501 acatccccct ttcgccagct ggcgtaatag cgaagaggcc cgcaccgatc gcccttccca 1561 acagttgcgc agcctgaatg gcgaatggaa attgtaagcg ttaatatttt gttaaaattc 1621 gcgttaaatt tttgttaaat cagctcattt tttaaccaat aggccgaaat cggcaaaatc 1681 ccttataaat caaaagaata gaccgagata gggttgagtg ttgttccagt ttggaacaag 1741 agtccactat taaagaacgt ggactccaac gtcaaagggc gaaaaaccgt ctatcagggc 1801 gatggcccac tacgtgaacc atcaccctaa tcaagttttt tggggtcgag gtgccgtaaa 1861 gcactaaatc ggaaccctaa agggagcccc cgatttagag cttgacgggg aaagccggcg 1921 aacgtggcga gaaaggaagg gaagaaagcg aaaggagcgg gcgctagggc gctggcaagt 1981 gtagcggtca cgctgcgcgt aaccaccaca cccgccgcgc ttaatgcgcc gctacagggc 2041 gcgtcaggtg gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt atttttctaa 2101 atacattcaa atatgtatcc gctcatgaga caataaccct gataaatgct tcaataatat 2161 tgaaaaagga agagtatgag tattcaacat ttccgtgtcg cccttattcc cttttttgcg 2221 gcattttgcc ttcctgtttt tgctcaccca gaaacgctgg tgaaagtaaa agatgctgaa 2281 gatcagttgg gtgcacgagt gggttacatc gaactggatc tcaacagcgg taagatcctt 2341 gagagttttc gccccgaaga acgttttcca atgatgagca cttttaaagt tctgctatgt 2401 ggcgcggtat tatcccgtat tgacgccggg caagagcaac tcggtcgccg catacactat 2461 tctcagaatg acttggttga gtactcacca gtcacagaaa agcatcttac ggatggcatg 2521 acagtaagag aattatgcag tgctgccata accatgagtg ataacactgc ggccaactta 2581 cttctgacaa cgatcggagg accgaaggag ctaaccgctt ttttgcacaa catgggggat 2641 catgtaactc gccttgatcg ttgggaaccg gagctgaatg aagccatacc aaacgacgag 2701 cgtgacacca cgatgcctgt agcaatggca acaacgttgc gcaaactatt aactggcgaa 2761 ctacttactc tagcttcccg gcaacaatta atagactgga tggaggcgga taaagttgca 2821 ggaccacttc tgcgctcggc ccttccggct ggctggttta ttgctgataa atctggagcc 2881 ggtgagcgtg ggtctcgcgg tatcattgca gcactggggc cagatggtaa gccctcccgt 2941 atcgtagtta tctacacgac ggggagtcag gcaactatgg atgaacgaaa tagacagatc 3001 gctgagatag gtgcctcact gattaagcat tggtaactgt cagaccaagt ttactcatat 3061 atactttaga ttgatttaaa acttcatttt taatttaaaa ggatctaggt gaagatcctt 3121 tttgataatc tcatgaccaa aatcccttaa cgtgagtttt cgttccactg agcgtcagac 3181 cccgtagaaa agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc 3241 ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca 3301 actctttttc cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgtccttcta 3361 gtgtagccgt agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct 3421 ctgctaatcc tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg 3481 gactcaagac gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc 3541 acacagccca gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta 3601 tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg 3661 gtcggaacag gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt 3721 cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg 3781 cggagcctat ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg 3841 ccttttgctc acatgttctt tcctgcgtta tcccctgatt ctgtggataa ccgtattacc 3901 gcctttgagt gagctgatac cgctcgccgc agccgaacga ccgagcgcag cgagtcagtg 3961 agcgaggaag cggaagagcg cccaatacgc aaaccgcctc tccccgcgcg ttggccgatt 4021 cattaatgca gctggcacga caggtttccc gactggaaag cgggcagtga gcgcaacgca 4081 attaatgtga gttagctcac tcattaggca ccccaggctt tacactttat gcttccggct 4141 cgtatgttgt //