LOCUS       B444pmGFPD-ocs          4078 bp    DNA     circular SYN 22-JAN-2016
DEFINITION  Ligation of GFPdi-Hind-Sal* into pOCS-BM*
ACCESSION   B444pmGFPD-ocs
KEYWORDS    .
SOURCE      Unknown.
  ORGANISM  Unknown
            Unclassified.
REFERENCE   1  (bases 1 to 4078)
  AUTHORS   Self
  JOURNAL   Unpublished.
COMMENT     SECID/File created by Clone Manager, Scientific & Educational Software
COMMENT     SECNOTES|Vector molecule:  pOCS-BM*
               Fragment ends: SalI and HindIII
               Fragment size: 3146
            Insert molecule:  GFPdi-Hind-Sal*
               Fragment ends: HindIII and SalI
               Fragment size: 932
            Primer for gene synthesis
            DHindGFP	cccgtcAAGCTTACGCGtcctccataactATGGGCAAGGGC
            SalGFP	CCCGTGgtcgaCGAGTCACTTGTAGAG
            Sequencing primers	
            OR2	GTGCACAACAGAATTGAAAGCAAA
FEATURES             Location/Qualifiers
     CDS             81..459
                     /gene="gfp"
                     /product="GFP"
                     /SECDrawAs="Gene"
                     /SECStyleId=1
     CDS             460..648
                     /gene="int"
                     /product="STLS1"
                     /SECDrawAs="Gene"
                     /SECStyleId=1
     CDS             649..986
                     /gene="gfp"
                     /product="GFP"
                     /SECDrawAs="Gene"
                     /SECStyleId=1
     misc_feature    995..1190
                     /gene="Tocs"
                     /product="Terminator"
                     /SECDrawAs="Region"
                     /SECStyleId=1
                     /SECName="Tocs"
                     /SECDescr="Terminator"
     misc_feature    2006..2866
                     /gene="Amp"
                     /product="ORF-1"
                     /SECDrawAs="Gene"
                     /SECStyleId=1
                     /SECName="Amp"
                     /SECDescr="ORF-1"
     CDS             2909..3625
                     /gene="ColE1"
                     /product="ColE1"
                     /SECDrawAs="Region"
                     /SECStyleId=1
                     /SECName="ColE1"
                     /SECDescr="ColE1"
ORIGIN      
        1 gagctctcta gaggccatgg cggccgcact agtggatccc ccgggctgca ggaattcaag
       61 cttacgcgtc ctccataact atgggcaagg gcgaggaact gttcactggc gtggtcccaa
      121 tcctggtgga actggatggt gatgtgaacg ggcacaagtt ctccgtcagc ggagagggtg
      181 aaggtgatgc cacctacgga aagctcaccc tgaagttcat ctgcactacc ggaaagctcc
      241 ctgttccgtg gccaaccctc gtcaccactt tcacctacgg tgttcagtgc ttctcccggt
      301 acccagatca catgaagcag catgacttct tcaagagcgc catgcccgaa ggctacgtgc
      361 aagaaaggac tatcttcttc aaggatgacg ggaactacaa gacacgtgcc gaagtcaagt
      421 tcgaaggtga taccctggtg aaccgcatcg agctgaaagg taagtttctg cttctacctt
      481 tgatatatat ataataatta tcattaatta gtagtaatat aatatttcaa atattttttt
      541 caaaataaaa gaatgtagta tatagcaatt gcttttctgt agtttataag tgtgtatatt
      601 ttaatttata acttttctaa tatatgacca aaatttgttg atgtgcaggt atcgatttca
      661 aggaagatgg aaacatcctc ggacacaagc tggagtacaa ctacaactcc cacaacgtat
      721 acatcatggc cgacaagcag aagaacggca tcaaggtgaa cttcaagatc aggcacaaca
      781 tcgaagatgg aagcgtgcaa ctggcggacc actaccagca gaacacgccc atcggcgatg
      841 gccctgtcct gctgccggac aaccattacc tgtccacgca atctgccctc tccaaggacc
      901 ccaacgagaa gagggaccac atggtcctgc tggagttcgt gacggctgct gggatcacgc
      961 atggcatgga tgaactctac aagtgactcg tcgaccctgc tttaatgaga tatgcgagac
     1021 gcctatgatc gcatgatatt tgctttcaat tctgttgtgc acgttgtaaa aaacctgagc
     1081 atgtgtagct cagatcctta ccgccggttt cggttcattc taatgaatat atcacccgtt
     1141 actatcgtat ttttatgaat aatattctcc gttcaattta ctgattgtcc tcgacaggcc
     1201 ttaagggcca gatcttgggc ccggtaccca attcgcccta tagtgagtcg tattacaatt
     1261 cactggccgt cgttttacaa cgtcgtgact gggaaaaccc tggcgttacc caacttaatc
     1321 gccttgcagc acatccccct ttcgccagct ggcgtaatag cgaagaggcc cgcaccgatc
     1381 gcccttccca acagttgcgc agcctgaatg gcgaatggaa attgtaagcg ttaatatttt
     1441 gttaaaattc gcgttaaatt tttgttaaat cagctcattt tttaaccaat aggccgaaat
     1501 cggcaaaatc ccttataaat caaaagaata gaccgagata gggttgagtg ttgttccagt
     1561 ttggaacaag agtccactat taaagaacgt ggactccaac gtcaaagggc gaaaaaccgt
     1621 ctatcagggc gatggcccac tacgtgaacc atcaccctaa tcaagttttt tggggtcgag
     1681 gtgccgtaaa gcactaaatc ggaaccctaa agggagcccc cgatttagag cttgacgggg
     1741 aaagccggcg aacgtggcga gaaaggaagg gaagaaagcg aaaggagcgg gcgctagggc
     1801 gctggcaagt gtagcggtca cgctgcgcgt aaccaccaca cccgccgcgc ttaatgcgcc
     1861 gctacagggc gcgtcaggtg gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt
     1921 atttttctaa atacattcaa atatgtatcc gctcatgaga caataaccct gataaatgct
     1981 tcaataatat tgaaaaagga agagtatgag tattcaacat ttccgtgtcg cccttattcc
     2041 cttttttgcg gcattttgcc ttcctgtttt tgctcaccca gaaacgctgg tgaaagtaaa
     2101 agatgctgaa gatcagttgg gtgcacgagt gggttacatc gaactggatc tcaacagcgg
     2161 taagatcctt gagagttttc gccccgaaga acgttttcca atgatgagca cttttaaagt
     2221 tctgctatgt ggcgcggtat tatcccgtat tgacgccggg caagagcaac tcggtcgccg
     2281 catacactat tctcagaatg acttggttga gtactcacca gtcacagaaa agcatcttac
     2341 ggatggcatg acagtaagag aattatgcag tgctgccata accatgagtg ataacactgc
     2401 ggccaactta cttctgacaa cgatcggagg accgaaggag ctaaccgctt ttttgcacaa
     2461 catgggggat catgtaactc gccttgatcg ttgggaaccg gagctgaatg aagccatacc
     2521 aaacgacgag cgtgacacca cgatgcctgt agcaatggca acaacgttgc gcaaactatt
     2581 aactggcgaa ctacttactc tagcttcccg gcaacaatta atagactgga tggaggcgga
     2641 taaagttgca ggaccacttc tgcgctcggc ccttccggct ggctggttta ttgctgataa
     2701 atctggagcc ggtgagcgtg ggtctcgcgg tatcattgca gcactggggc cagatggtaa
     2761 gccctcccgt atcgtagtta tctacacgac ggggagtcag gcaactatgg atgaacgaaa
     2821 tagacagatc gctgagatag gtgcctcact gattaagcat tggtaactgt cagaccaagt
     2881 ttactcatat atactttaga ttgatttaaa acttcatttt taatttaaaa ggatctaggt
     2941 gaagatcctt tttgataatc tcatgaccaa aatcccttaa cgtgagtttt cgttccactg
     3001 agcgtcagac cccgtagaaa agatcaaagg atcttcttga gatccttttt ttctgcgcgt
     3061 aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt tgccggatca
     3121 agagctacca actctttttc cgaaggtaac tggcttcagc agagcgcaga taccaaatac
     3181 tgtccttcta gtgtagccgt agttaggcca ccacttcaag aactctgtag caccgcctac
     3241 atacctcgct ctgctaatcc tgttaccagt ggctgctgcc agtggcgata agtcgtgtct
     3301 taccgggttg gactcaagac gatagttacc ggataaggcg cagcggtcgg gctgaacggg
     3361 gggttcgtgc acacagccca gcttggagcg aacgacctac accgaactga gatacctaca
     3421 gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca ggtatccggt
     3481 aagcggcagg gtcggaacag gagagcgcac gagggagctt ccagggggaa acgcctggta
     3541 tctttatagt cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc
     3601 gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc
     3661 cttttgctgg ccttttgctc acatgttctt tcctgcgtta tcccctgatt ctgtggataa
     3721 ccgtattacc gcctttgagt gagctgatac cgctcgccgc agccgaacga ccgagcgcag
     3781 cgagtcagtg agcgaggaag cggaagagcg cccaatacgc aaaccgcctc tccccgcgcg
     3841 ttggccgatt cattaatgca gctggcacga caggtttccc gactggaaag cgggcagtga
     3901 gcgcaacgca attaatgtga gttagctcac tcattaggca ccccaggctt tacactttat
     3961 gcttccggct cgtatgttgt gtggaattgt gagcggataa caatttcaca caggaaacag
     4021 ctatgaccat gattacgcca agctcgaaat taaccctcac taaagggaac aaaagctg
//
