LOCUS       B589p35s-mGFPD-o        4489 bp    DNA     circular SYN 22-JAN-2016
DEFINITION  Ligation of B444pmGFPD-ocs* into B491p35s-DoGusi-ocs*
ACCESSION   B589p35s-mGFPD-o
KEYWORDS    .
SOURCE      Unknown.
  ORGANISM  Unknown
            Unclassified.
REFERENCE   1  (bases 1 to 4489)
  AUTHORS   Self
  JOURNAL   Unpublished.
COMMENT     SECID/File created by Clone Manager, Scientific & Educational Software
COMMENT     SECNOTES|Vector molecule:  B491p35s-DoGusi-ocs*
               Fragment ends: SalI and HindIII
               Fragment size: 3557
            Insert molecule:  B444pmGFPD-ocs*
               Fragment ends: HindIII and SalI
               Fragment size: 932
            GELR
            Sequencing primers	
            OR2	GTGCACAACAGAATTGAAAGCAAA
FEATURES             Location/Qualifiers
     misc_feature    144..562
                     /gene="P35S"
                     /product="Promotor-Region"
                     /SECDrawAs="Region"
                     /SECStyleId=1
                     /SECName="P35S"
                     /SECDescr="Promotor-Region"
     misc_signal     473..562
                     /label=Minimal promotor
                     /SECDrawAs="Label"
     misc_feature    563..564
                     /product="T-start"
                     /SECDrawAs="Region"
                     /SECStyleId=1
     CDS             590..968
                     /gene="gfp"
                     /product="GFP"
                     /SECDrawAs="Gene"
                     /SECStyleId=1
     TATA_signal     969..1157
                     /gene="int"
                     /product="STLS1"
                     /SECDrawAs="Gene"
                     /SECStyleId=1
                     /SECName="int"
                     /SECDescr="STLS1"
     CDS             1158..1495
                     /gene="gfp"
                     /product="GFP"
                     /SECDrawAs="Gene"
                     /SECStyleId=1
     misc_feature    1504..1699
                     /gene="Tocs"
                     /product="Terminator"
                     /SECDrawAs="Region"
                     /SECStyleId=1
                     /SECName="Tocs"
                     /SECDescr="Terminator"
     misc_feature    2515..3375
                     /gene="Amp"
                     /product="ORF-1"
                     /SECDrawAs="Gene"
                     /SECStyleId=1
                     /SECName="Amp"
                     /SECDescr="ORF-1"
     CDS             3418..4134
                     /gene="ColE1"
                     /product="ColE1"
                     /SECDrawAs="Region"
                     /SECStyleId=1
                     /SECName="ColE1"
                     /SECDescr="ColE1"
ORIGIN      
        1 gtggaattgt gagcggataa caatttcaca caggaaacag ctatgaccat gattacgcca
       61 agctcgaaat taaccctcac taaagggaac aaaagctgga gctctctaga ggccatggcg
      121 gccgcactag tggatccccc gggtcaacat ggtggagcac gacactctcg tctactccaa
      181 gaatatcaaa gatacagtct cagaagacca gagggctatt gagacttttc aacaaagggt
      241 aatatcggga aacctcctcg gattccattg cccagctatc tgtcacttca tcgaaaggac
      301 agtagaaaag gaagatggct tctacaaatg ccatcattgc gataaaggaa aggctatcgt
      361 tcaagatgcc tctaccgaca gtggtcccaa agatggaccc ccacccacga ggaacatcgt
      421 ggaaaaagaa gacgttccaa ccacgtcttc aaagcaagtg gattgatgtg atatctccac
      481 tgacgtaagg gatgacgcac aatcccacta tccttcgcaa gacccttcct ctatataagg
      541 aagttcattt catttggaga ggacacaagc ttacgcgtcc tccataacta tgggcaaggg
      601 cgaggaactg ttcactggcg tggtcccaat cctggtggaa ctggatggtg atgtgaacgg
      661 gcacaagttc tccgtcagcg gagagggtga aggtgatgcc acctacggaa agctcaccct
      721 gaagttcatc tgcactaccg gaaagctccc tgttccgtgg ccaaccctcg tcaccacttt
      781 cacctacggt gttcagtgct tctcccggta cccagatcac atgaagcagc atgacttctt
      841 caagagcgcc atgcccgaag gctacgtgca agaaaggact atcttcttca aggatgacgg
      901 gaactacaag acacgtgccg aagtcaagtt cgaaggtgat accctggtga accgcatcga
      961 gctgaaaggt aagtttctgc ttctaccttt gatatatata taataattat cattaattag
     1021 tagtaatata atatttcaaa tatttttttc aaaataaaag aatgtagtat atagcaattg
     1081 cttttctgta gtttataagt gtgtatattt taatttataa cttttctaat atatgaccaa
     1141 aatttgttga tgtgcaggta tcgatttcaa ggaagatgga aacatcctcg gacacaagct
     1201 ggagtacaac tacaactccc acaacgtata catcatggcc gacaagcaga agaacggcat
     1261 caaggtgaac ttcaagatca ggcacaacat cgaagatgga agcgtgcaac tggcggacca
     1321 ctaccagcag aacacgccca tcggcgatgg ccctgtcctg ctgccggaca accattacct
     1381 gtccacgcaa tctgccctct ccaaggaccc caacgagaag agggaccaca tggtcctgct
     1441 ggagttcgtg acggctgctg ggatcacgca tggcatggat gaactctaca agtgactcgt
     1501 cgaccctgct ttaatgagat atgcgagacg cctatgatcg catgatattt gctttcaatt
     1561 ctgttgtgca cgttgtaaaa aacctgagca tgtgtagctc agatccttac cgccggtttc
     1621 ggttcattct aatgaatata tcacccgtta ctatcgtatt tttatgaata atattctccg
     1681 ttcaatttac tgattgtcct cgacaggcct taagggccag atcttgggcc cggtacccaa
     1741 ttcgccctat agtgagtcgt attacaattc actggccgtc gttttacaac gtcgtgactg
     1801 ggaaaaccct ggcgttaccc aacttaatcg ccttgcagca catccccctt tcgccagctg
     1861 gcgtaatagc gaagaggccc gcaccgatcg cccttcccaa cagttgcgca gcctgaatgg
     1921 cgaatggaaa ttgtaagcgt taatattttg ttaaaattcg cgttaaattt ttgttaaatc
     1981 agctcatttt ttaaccaata ggccgaaatc ggcaaaatcc cttataaatc aaaagaatag
     2041 accgagatag ggttgagtgt tgttccagtt tggaacaaga gtccactatt aaagaacgtg
     2101 gactccaacg tcaaagggcg aaaaaccgtc tatcagggcg atggcccact acgtgaacca
     2161 tcaccctaat caagtttttt ggggtcgagg tgccgtaaag cactaaatcg gaaccctaaa
     2221 gggagccccc gatttagagc ttgacgggga aagccggcga acgtggcgag aaaggaaggg
     2281 aagaaagcga aaggagcggg cgctagggcg ctggcaagtg tagcggtcac gctgcgcgta
     2341 accaccacac ccgccgcgct taatgcgccg ctacagggcg cgtcaggtgg cacttttcgg
     2401 ggaaatgtgc gcggaacccc tatttgttta tttttctaaa tacattcaaa tatgtatccg
     2461 ctcatgagac aataaccctg ataaatgctt caataatatt gaaaaaggaa gagtatgagt
     2521 attcaacatt tccgtgtcgc ccttattccc ttttttgcgg cattttgcct tcctgttttt
     2581 gctcacccag aaacgctggt gaaagtaaaa gatgctgaag atcagttggg tgcacgagtg
     2641 ggttacatcg aactggatct caacagcggt aagatccttg agagttttcg ccccgaagaa
     2701 cgttttccaa tgatgagcac ttttaaagtt ctgctatgtg gcgcggtatt atcccgtatt
     2761 gacgccgggc aagagcaact cggtcgccgc atacactatt ctcagaatga cttggttgag
     2821 tactcaccag tcacagaaaa gcatcttacg gatggcatga cagtaagaga attatgcagt
     2881 gctgccataa ccatgagtga taacactgcg gccaacttac ttctgacaac gatcggagga
     2941 ccgaaggagc taaccgcttt tttgcacaac atgggggatc atgtaactcg ccttgatcgt
     3001 tgggaaccgg agctgaatga agccatacca aacgacgagc gtgacaccac gatgcctgta
     3061 gcaatggcaa caacgttgcg caaactatta actggcgaac tacttactct agcttcccgg
     3121 caacaattaa tagactggat ggaggcggat aaagttgcag gaccacttct gcgctcggcc
     3181 cttccggctg gctggtttat tgctgataaa tctggagccg gtgagcgtgg gtctcgcggt
     3241 atcattgcag cactggggcc agatggtaag ccctcccgta tcgtagttat ctacacgacg
     3301 gggagtcagg caactatgga tgaacgaaat agacagatcg ctgagatagg tgcctcactg
     3361 attaagcatt ggtaactgtc agaccaagtt tactcatata tactttagat tgatttaaaa
     3421 cttcattttt aatttaaaag gatctaggtg aagatccttt ttgataatct catgaccaaa
     3481 atcccttaac gtgagttttc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga
     3541 tcttcttgag atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg
     3601 ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact
     3661 ggcttcagca gagcgcagat accaaatact gtccttctag tgtagccgta gttaggccac
     3721 cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg
     3781 gctgctgcca gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg
     3841 gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga
     3901 acgacctaca ccgaactgag atacctacag cgtgagctat gagaaagcgc cacgcttccc
     3961 gaagggagaa aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg
     4021 agggagcttc cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc
     4081 tgacttgagc gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc
     4141 agcaacgcgg cctttttacg gttcctggcc ttttgctggc cttttgctca catgttcttt
     4201 cctgcgttat cccctgattc tgtggataac cgtattaccg cctttgagtg agctgatacc
     4261 gctcgccgca gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc ggaagagcgc
     4321 ccaatacgca aaccgcctct ccccgcgcgt tggccgattc attaatgcag ctggcacgac
     4381 aggtttcccg actggaaagc gggcagtgag cgcaacgcaa ttaatgtgag ttagctcact
     4441 cattaggcac cccaggcttt acactttatg cttccggctc gtatgttgt
//
