LOCUS B590pNos-mGFPD-o 4368 bp DNA circular SYN 08-FEB-2016 DEFINITION Ligation of B444pmGFPD-ocs* into B492pNos-DoGusi-ocs* ACCESSION B590pNos-mGFPD-o KEYWORDS . SOURCE Unknown. ORGANISM Unknown Unclassified. REFERENCE 1 (bases 1 to 4368) AUTHORS Self JOURNAL Unpublished. COMMENT SECID/File created by Clone Manager, Scientific & Educational Software COMMENT SECNOTES|Vector molecule: B492pNos-DoGusi-ocs* Fragment ends: SalI and HindIII Fragment size: 3436 Insert molecule: B444pmGFPD-ocs* Fragment ends: HindIII and SalI Fragment size: 932 GELR Sequencing primers OR2 GTGCACAACAGAATTGAAAGCAAA FEATURES Location/Qualifiers misc_feature 145..442 /gene="Pnos" /product="Promotor" /SECDrawAs="Region" /SECStyleId=1 /SECName="Pnos" /SECDescr="Promotor" CDS 469..847 /gene="gfp" /product="GFP" /SECDrawAs="Gene" /SECStyleId=1 CDS 848..1036 /gene="int" /product="STLS1" /SECDrawAs="Gene" /SECStyleId=1 CDS 1037..1374 /gene="gfp" /product="GFP" /SECDrawAs="Gene" /SECStyleId=1 misc_feature 1383..1578 /gene="Tocs" /product="Terminator" /SECDrawAs="Region" /SECStyleId=1 /SECName="Tocs" /SECDescr="Terminator" misc_feature 2394..3254 /gene="Amp" /product="ORF-1" /SECDrawAs="Gene" /SECStyleId=1 /SECName="Amp" /SECDescr="ORF-1" CDS 3297..4013 /gene="ColE1" /product="ColE1" /SECDrawAs="Region" /SECStyleId=1 /SECName="ColE1" /SECDescr="ColE1" ORIGIN 1 gtggaattgt gagcggataa caatttcaca caggaaacag ctatgaccat gattacgcca 61 agctcgaaat taaccctcac taaagggaac aaaagctgga gctctctaga ggccatggcg 121 gccgcactag tggatccccc gggatcatga gcggagaatt aagggagtca cgttatgacc 181 cccgccgatg acgcgggaca agccgtttta cgtttggaac tgacagaacc gcaacgttga 241 aggagccact cagccgcggg tttctggagt ttaatgagct aagcacatac gtcagaaacc 301 attattgcgc gttcaaaagt cgcctaaggt cactatcagc tagcaaatat ttcttgtcaa 361 aaatgctcca ctgacgttcc ataaattccc ctcggtatcc aattagagtc tcatattcac 421 tctcaatcca aataatctgc acctcaagct tacgcgtcct ccataactat gggcaagggc 481 gaggaactgt tcactggcgt ggtcccaatc ctggtggaac tggatggtga tgtgaacggg 541 cacaagttct ccgtcagcgg agagggtgaa ggtgatgcca cctacggaaa gctcaccctg 601 aagttcatct gcactaccgg aaagctccct gttccgtggc caaccctcgt caccactttc 661 acctacggtg ttcagtgctt ctcccggtac ccagatcaca tgaagcagca tgacttcttc 721 aagagcgcca tgcccgaagg ctacgtgcaa gaaaggacta tcttcttcaa ggatgacggg 781 aactacaaga cacgtgccga agtcaagttc gaaggtgata ccctggtgaa ccgcatcgag 841 ctgaaaggta agtttctgct tctacctttg atatatatat aataattatc attaattagt 901 agtaatataa tatttcaaat atttttttca aaataaaaga atgtagtata tagcaattgc 961 ttttctgtag tttataagtg tgtatatttt aatttataac ttttctaata tatgaccaaa 1021 atttgttgat gtgcaggtat cgatttcaag gaagatggaa acatcctcgg acacaagctg 1081 gagtacaact acaactccca caacgtatac atcatggccg acaagcagaa gaacggcatc 1141 aaggtgaact tcaagatcag gcacaacatc gaagatggaa gcgtgcaact ggcggaccac 1201 taccagcaga acacgcccat cggcgatggc cctgtcctgc tgccggacaa ccattacctg 1261 tccacgcaat ctgccctctc caaggacccc aacgagaaga gggaccacat ggtcctgctg 1321 gagttcgtga cggctgctgg gatcacgcat ggcatggatg aactctacaa gtgactcgtc 1381 gaccctgctt taatgagata tgcgagacgc ctatgatcgc atgatatttg ctttcaattc 1441 tgttgtgcac gttgtaaaaa acctgagcat gtgtagctca gatccttacc gccggtttcg 1501 gttcattcta atgaatatat cacccgttac tatcgtattt ttatgaataa tattctccgt 1561 tcaatttact gattgtcctc gacaggcctt aagggccaga tcttgggccc ggtacccaat 1621 tcgccctata gtgagtcgta ttacaattca ctggccgtcg ttttacaacg tcgtgactgg 1681 gaaaaccctg gcgttaccca acttaatcgc cttgcagcac atcccccttt cgccagctgg 1741 cgtaatagcg aagaggcccg caccgatcgc ccttcccaac agttgcgcag cctgaatggc 1801 gaatggaaat tgtaagcgtt aatattttgt taaaattcgc gttaaatttt tgttaaatca 1861 gctcattttt taaccaatag gccgaaatcg gcaaaatccc ttataaatca aaagaataga 1921 ccgagatagg gttgagtgtt gttccagttt ggaacaagag tccactatta aagaacgtgg 1981 actccaacgt caaagggcga aaaaccgtct atcagggcga tggcccacta cgtgaaccat 2041 caccctaatc aagttttttg gggtcgaggt gccgtaaagc actaaatcgg aaccctaaag 2101 ggagcccccg atttagagct tgacggggaa agccggcgaa cgtggcgaga aaggaaggga 2161 agaaagcgaa aggagcgggc gctagggcgc tggcaagtgt agcggtcacg ctgcgcgtaa 2221 ccaccacacc cgccgcgctt aatgcgccgc tacagggcgc gtcaggtggc acttttcggg 2281 gaaatgtgcg cggaacccct atttgtttat ttttctaaat acattcaaat atgtatccgc 2341 tcatgagaca ataaccctga taaatgcttc aataatattg aaaaaggaag agtatgagta 2401 ttcaacattt ccgtgtcgcc cttattccct tttttgcggc attttgcctt cctgtttttg 2461 ctcacccaga aacgctggtg aaagtaaaag atgctgaaga tcagttgggt gcacgagtgg 2521 gttacatcga actggatctc aacagcggta agatccttga gagttttcgc cccgaagaac 2581 gttttccaat gatgagcact tttaaagttc tgctatgtgg cgcggtatta tcccgtattg 2641 acgccgggca agagcaactc ggtcgccgca tacactattc tcagaatgac ttggttgagt 2701 actcaccagt cacagaaaag catcttacgg atggcatgac agtaagagaa ttatgcagtg 2761 ctgccataac catgagtgat aacactgcgg ccaacttact tctgacaacg atcggaggac 2821 cgaaggagct aaccgctttt ttgcacaaca tgggggatca tgtaactcgc cttgatcgtt 2881 gggaaccgga gctgaatgaa gccataccaa acgacgagcg tgacaccacg atgcctgtag 2941 caatggcaac aacgttgcgc aaactattaa ctggcgaact acttactcta gcttcccggc 3001 aacaattaat agactggatg gaggcggata aagttgcagg accacttctg cgctcggccc 3061 ttccggctgg ctggtttatt gctgataaat ctggagccgg tgagcgtggg tctcgcggta 3121 tcattgcagc actggggcca gatggtaagc cctcccgtat cgtagttatc tacacgacgg 3181 ggagtcaggc aactatggat gaacgaaata gacagatcgc tgagataggt gcctcactga 3241 ttaagcattg gtaactgtca gaccaagttt actcatatat actttagatt gatttaaaac 3301 ttcattttta atttaaaagg atctaggtga agatcctttt tgataatctc atgaccaaaa 3361 tcccttaacg tgagttttcg ttccactgag cgtcagaccc cgtagaaaag atcaaaggat 3421 cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc 3481 taccagcggt ggtttgtttg ccggatcaag agctaccaac tctttttccg aaggtaactg 3541 gcttcagcag agcgcagata ccaaatactg tccttctagt gtagccgtag ttaggccacc 3601 acttcaagaa ctctgtagca ccgcctacat acctcgctct gctaatcctg ttaccagtgg 3661 ctgctgccag tggcgataag tcgtgtctta ccgggttgga ctcaagacga tagttaccgg 3721 ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac acagcccagc ttggagcgaa 3781 cgacctacac cgaactgaga tacctacagc gtgagctatg agaaagcgcc acgcttcccg 3841 aagggagaaa ggcggacagg tatccggtaa gcggcagggt cggaacagga gagcgcacga 3901 gggagcttcc agggggaaac gcctggtatc tttatagtcc tgtcgggttt cgccacctct 3961 gacttgagcg tcgatttttg tgatgctcgt caggggggcg gagcctatgg aaaaacgcca 4021 gcaacgcggc ctttttacgg ttcctggcct tttgctggcc ttttgctcac atgttctttc 4081 ctgcgttatc ccctgattct gtggataacc gtattaccgc ctttgagtga gctgataccg 4141 ctcgccgcag ccgaacgacc gagcgcagcg agtcagtgag cgaggaagcg gaagagcgcc 4201 caatacgcaa accgcctctc cccgcgcgtt ggccgattca ttaatgcagc tggcacgaca 4261 ggtttcccga ctggaaagcg ggcagtgagc gcaacgcaat taatgtgagt tagctcactc 4321 attaggcacc ccaggcttta cactttatgc ttccggctcg tatgttgt //