LOCUS       B591pUbiHa-mGFPD        4406 bp    DNA     circular SYN 08-FEB-2016
DEFINITION  Ligation of B444pmGFPD-ocs* into B493pUbiHa-DoGusi-ocs*
ACCESSION   B591pUbiHa-mGFPD
KEYWORDS    .
SOURCE      Unknown.
  ORGANISM  Unknown
            Unclassified.
REFERENCE   1  (bases 1 to 4406)
  AUTHORS   Self
  JOURNAL   Unpublished.
COMMENT     SECID/File created by Clone Manager, Scientific & Educational Software
COMMENT     SECNOTES|Vector molecule:  B493pUbiHa-DoGusi-ocs*
               Fragment ends: SalI and HindIII
               Fragment size: 3474
            Insert molecule:  B444pmGFPD-ocs*
               Fragment ends: HindIII and SalI
               Fragment size: 932
            GELR
            Sequencing primers:
            Xba     nur Mini
            T7QL     nur Maxi
FEATURES             Location/Qualifiers
     misc_feature    146..481
                     /gene="UbiHa"
                     /product="Promotor"
                     /SECDrawAs="Region"
                     /SECStyleId=1
     misc_signal     263..265
                     /label=cat
                     /SECDrawAs="Label"
     misc_signal     266..268
                     /label=cat
                     /SECDrawAs="Label"
     misc_signal     299..301
                     /label=cat
                     /SECDrawAs="Label"
     misc_signal     328..330
                     /label=cat
                     /SECDrawAs="Label"
     misc_signal     339..341
                     /label=cat
                     /SECDrawAs="Label"
     misc_signal     360..362
                     /label=cat
                     /SECDrawAs="Label"
     misc_signal     363..365
                     /label=cat
                     /SECDrawAs="Label"
     misc_signal     368..371
                     /label=tata
                     /SECDrawAs="Label"
     CDS             399..481
                     /gene="mRNA"
                     /SECDrawAs="Gene"
                     /SECStyleId=1
     CDS             507..885
                     /gene="gfp"
                     /product="GFP"
                     /SECDrawAs="Gene"
                     /SECStyleId=1
     CDS             886..1074
                     /gene="int"
                     /product="STLS1"
                     /SECDrawAs="Gene"
                     /SECStyleId=1
     CDS             1075..1412
                     /gene="gfp"
                     /product="GFP"
                     /SECDrawAs="Gene"
                     /SECStyleId=1
     misc_feature    1421..1616
                     /gene="Tocs"
                     /product="Terminator"
                     /SECDrawAs="Region"
                     /SECStyleId=1
                     /SECName="Tocs"
                     /SECDescr="Terminator"
     misc_feature    2432..3292
                     /gene="Amp"
                     /product="ORF-1"
                     /SECDrawAs="Gene"
                     /SECStyleId=1
                     /SECName="Amp"
                     /SECDescr="ORF-1"
     CDS             3335..4051
                     /gene="ColE1"
                     /product="ColE1"
                     /SECDrawAs="Region"
                     /SECStyleId=1
                     /SECName="ColE1"
                     /SECDescr="ColE1"
ORIGIN      
        1 gtggaattgt gagcggataa caatttcaca caggaaacag ctatgaccat gattacgcca
       61 agctcgaaat taaccctcac taaagggaac aaaagctgga gctctctaga ggccatggcg
      121 gccgcactag tggatccccc gggacaaaac ttaagaaagt caccaaaaag aaagtcaaag
      181 acggcaacgg caacccagaa gctacgttct ctttctagat tctatgccct acctaccgcg
      241 tggtgcacaa caacttgacc cccatcattg actctccacc gtacacgtgt cacgatacca
      301 tttgttggtc tcctaccact tacgtaacat taatattcca tccgtgaccg cctcttcttc
      361 atcatcctat aaattcacct tcttatctct tccgctttca ttcgttcatc atcatcacaa
      421 ttaacaacaa tccaaagcaa tcttctctca tattcttatc tcttcctgtt tcgcttcgtc
      481 tacaagctta cgcgtcctcc ataactatgg gcaagggcga ggaactgttc actggcgtgg
      541 tcccaatcct ggtggaactg gatggtgatg tgaacgggca caagttctcc gtcagcggag
      601 agggtgaagg tgatgccacc tacggaaagc tcaccctgaa gttcatctgc actaccggaa
      661 agctccctgt tccgtggcca accctcgtca ccactttcac ctacggtgtt cagtgcttct
      721 cccggtaccc agatcacatg aagcagcatg acttcttcaa gagcgccatg cccgaaggct
      781 acgtgcaaga aaggactatc ttcttcaagg atgacgggaa ctacaagaca cgtgccgaag
      841 tcaagttcga aggtgatacc ctggtgaacc gcatcgagct gaaaggtaag tttctgcttc
      901 tacctttgat atatatataa taattatcat taattagtag taatataata tttcaaatat
      961 ttttttcaaa ataaaagaat gtagtatata gcaattgctt ttctgtagtt tataagtgtg
     1021 tatattttaa tttataactt ttctaatata tgaccaaaat ttgttgatgt gcaggtatcg
     1081 atttcaagga agatggaaac atcctcggac acaagctgga gtacaactac aactcccaca
     1141 acgtatacat catggccgac aagcagaaga acggcatcaa ggtgaacttc aagatcaggc
     1201 acaacatcga agatggaagc gtgcaactgg cggaccacta ccagcagaac acgcccatcg
     1261 gcgatggccc tgtcctgctg ccggacaacc attacctgtc cacgcaatct gccctctcca
     1321 aggaccccaa cgagaagagg gaccacatgg tcctgctgga gttcgtgacg gctgctggga
     1381 tcacgcatgg catggatgaa ctctacaagt gactcgtcga ccctgcttta atgagatatg
     1441 cgagacgcct atgatcgcat gatatttgct ttcaattctg ttgtgcacgt tgtaaaaaac
     1501 ctgagcatgt gtagctcaga tccttaccgc cggtttcggt tcattctaat gaatatatca
     1561 cccgttacta tcgtattttt atgaataata ttctccgttc aatttactga ttgtcctcga
     1621 caggccttaa gggccagatc ttgggcccgg tacccaattc gccctatagt gagtcgtatt
     1681 acaattcact ggccgtcgtt ttacaacgtc gtgactggga aaaccctggc gttacccaac
     1741 ttaatcgcct tgcagcacat ccccctttcg ccagctggcg taatagcgaa gaggcccgca
     1801 ccgatcgccc ttcccaacag ttgcgcagcc tgaatggcga atggaaattg taagcgttaa
     1861 tattttgtta aaattcgcgt taaatttttg ttaaatcagc tcatttttta accaataggc
     1921 cgaaatcggc aaaatccctt ataaatcaaa agaatagacc gagatagggt tgagtgttgt
     1981 tccagtttgg aacaagagtc cactattaaa gaacgtggac tccaacgtca aagggcgaaa
     2041 aaccgtctat cagggcgatg gcccactacg tgaaccatca ccctaatcaa gttttttggg
     2101 gtcgaggtgc cgtaaagcac taaatcggaa ccctaaaggg agcccccgat ttagagcttg
     2161 acggggaaag ccggcgaacg tggcgagaaa ggaagggaag aaagcgaaag gagcgggcgc
     2221 tagggcgctg gcaagtgtag cggtcacgct gcgcgtaacc accacacccg ccgcgcttaa
     2281 tgcgccgcta cagggcgcgt caggtggcac ttttcgggga aatgtgcgcg gaacccctat
     2341 ttgtttattt ttctaaatac attcaaatat gtatccgctc atgagacaat aaccctgata
     2401 aatgcttcaa taatattgaa aaaggaagag tatgagtatt caacatttcc gtgtcgccct
     2461 tattcccttt tttgcggcat tttgccttcc tgtttttgct cacccagaaa cgctggtgaa
     2521 agtaaaagat gctgaagatc agttgggtgc acgagtgggt tacatcgaac tggatctcaa
     2581 cagcggtaag atccttgaga gttttcgccc cgaagaacgt tttccaatga tgagcacttt
     2641 taaagttctg ctatgtggcg cggtattatc ccgtattgac gccgggcaag agcaactcgg
     2701 tcgccgcata cactattctc agaatgactt ggttgagtac tcaccagtca cagaaaagca
     2761 tcttacggat ggcatgacag taagagaatt atgcagtgct gccataacca tgagtgataa
     2821 cactgcggcc aacttacttc tgacaacgat cggaggaccg aaggagctaa ccgctttttt
     2881 gcacaacatg ggggatcatg taactcgcct tgatcgttgg gaaccggagc tgaatgaagc
     2941 cataccaaac gacgagcgtg acaccacgat gcctgtagca atggcaacaa cgttgcgcaa
     3001 actattaact ggcgaactac ttactctagc ttcccggcaa caattaatag actggatgga
     3061 ggcggataaa gttgcaggac cacttctgcg ctcggccctt ccggctggct ggtttattgc
     3121 tgataaatct ggagccggtg agcgtgggtc tcgcggtatc attgcagcac tggggccaga
     3181 tggtaagccc tcccgtatcg tagttatcta cacgacgggg agtcaggcaa ctatggatga
     3241 acgaaataga cagatcgctg agataggtgc ctcactgatt aagcattggt aactgtcaga
     3301 ccaagtttac tcatatatac tttagattga tttaaaactt catttttaat ttaaaaggat
     3361 ctaggtgaag atcctttttg ataatctcat gaccaaaatc ccttaacgtg agttttcgtt
     3421 ccactgagcg tcagaccccg tagaaaagat caaaggatct tcttgagatc ctttttttct
     3481 gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc
     3541 ggatcaagag ctaccaactc tttttccgaa ggtaactggc ttcagcagag cgcagatacc
     3601 aaatactgtc cttctagtgt agccgtagtt aggccaccac ttcaagaact ctgtagcacc
     3661 gcctacatac ctcgctctgc taatcctgtt accagtggct gctgccagtg gcgataagtc
     3721 gtgtcttacc gggttggact caagacgata gttaccggat aaggcgcagc ggtcgggctg
     3781 aacggggggt tcgtgcacac agcccagctt ggagcgaacg acctacaccg aactgagata
     3841 cctacagcgt gagctatgag aaagcgccac gcttcccgaa gggagaaagg cggacaggta
     3901 tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg gagcttccag ggggaaacgc
     3961 ctggtatctt tatagtcctg tcgggtttcg ccacctctga cttgagcgtc gatttttgtg
     4021 atgctcgtca ggggggcgga gcctatggaa aaacgccagc aacgcggcct ttttacggtt
     4081 cctggccttt tgctggcctt ttgctcacat gttctttcct gcgttatccc ctgattctgt
     4141 ggataaccgt attaccgcct ttgagtgagc tgataccgct cgccgcagcc gaacgaccga
     4201 gcgcagcgag tcagtgagcg aggaagcgga agagcgccca atacgcaaac cgcctctccc
     4261 cgcgcgttgg ccgattcatt aatgcagctg gcacgacagg tttcccgact ggaaagcggg
     4321 cagtgagcgc aacgcaatta atgtgagtta gctcactcat taggcacccc aggctttaca
     4381 ctttatgctt ccggctcgta tgttgt
//
