LOCUS       B592pUbiAt-mGFPD        5544 bp    DNA     circular SYN 22-JAN-2016
DEFINITION  Ligation of B494pUbiAt-DoGusi-ocs* into B444pmGFPD-ocs*
ACCESSION   B592pUbiAt-mGFPD
KEYWORDS    .
SOURCE      Unknown.
  ORGANISM  Unknown
            Unclassified.
REFERENCE   1  (bases 1 to 5544)
  AUTHORS   Self
  JOURNAL   Unpublished.
COMMENT     SECID/File created by Clone Manager, Scientific & Educational Software
COMMENT     SECNOTES|Vector molecule:  B444pmGFPD-ocs*
               Fragment ends: HindIII and BamHI
               Fragment size: 4054
            Insert molecule:  B494pUbiAt-DoGusi-ocs*
               Fragment ends: BamHI and HindIII
               Fragment size: 1490
            GELR
            Sequencing primers	
            M13RY	CACACAGGAAACAGCTATGAC
            GFP-seq	CCAGTTCCACCAGGATTG
FEATURES             Location/Qualifiers
     misc_feature    46..1521
                     /gene="UBQ10"
                     /product="PUbq10At"
                     /SECDrawAs="Region"
                     /SECStyleId=1
     misc_feature    1150..1213
                     /product="Ubi cds 5"
                     /SECDrawAs="Region"
                     /SECStyleId=1
     misc_feature    1216..1519
                     /gene="int"
                     /SECDrawAs="Region"
                     /SECStyleId=1
     CDS             1547..1925
                     /gene="gfp"
                     /product="GFP"
                     /SECDrawAs="Gene"
                     /SECStyleId=1
     CDS             1926..2114
                     /gene="int"
                     /product="STLS1"
                     /SECDrawAs="Gene"
                     /SECStyleId=1
     CDS             2115..2452
                     /gene="gfp"
                     /product="GFP"
                     /SECDrawAs="Gene"
                     /SECStyleId=1
     misc_feature    2461..2656
                     /gene="Tocs"
                     /product="Terminator"
                     /SECDrawAs="Region"
                     /SECStyleId=1
                     /SECName="Tocs"
                     /SECDescr="Terminator"
     misc_feature    3472..4332
                     /gene="Amp"
                     /product="ORF-1"
                     /SECDrawAs="Gene"
                     /SECStyleId=1
                     /SECName="Amp"
                     /SECDescr="ORF-1"
     CDS             4375..5091
                     /gene="ColE1"
                     /product="ColE1"
                     /SECDrawAs="Region"
                     /SECStyleId=1
                     /SECName="ColE1"
                     /SECDescr="ColE1"
ORIGIN      
        1 gagctctcta gaggccatgg cggccgcact agtggatccc ccgggtttcc ttacattctg
       61 agcctctttc cttctaatcc actcatctgc atcttcttgt gtccttacta atacctcatt
      121 ggttccaaat tccctccctt taagcaccag ctcgtttctg ttcttccaca gcctcccaag
      181 tatccaaggg actaaagcct ccacattctt cagatcagga tattcttgtt taagatgttg
      241 aactctatgg aggtttgtat gaactgatga tctaggaccg gataagttcc cttcttcata
      301 gcgaacttat tcaaagaatg ttttgtgtat cattcttgtt acattgttat taatgaaaaa
      361 atattattgg tcattggact gaacacgagt gttaaatatg gaccaggccc caaataagat
      421 ccattgatat atgaattaaa taacaagaat aaatcgagtc accaaaccac ttgccttttt
      481 taacgagact tgttcaccaa cttgatacaa aagtcattat cctatgcaaa tcaataatca
      541 tacaaaaata tccaataaca ctaaaaaatt aaaagaaatg gataatttca caatatgtta
      601 tacgataaag aagttacttt tccaagaaat tcactgattt tataagccca cttgcattag
      661 ataaatggca aaaaaaaaca aaaaggaaaa gaaataaagc acgaagaatt ctagaaaata
      721 cgaaatacgc ttcaatgcag tgggacccac ggttcaatta ttgccaattt tcagctccac
      781 cgtatattta aaaaataaaa cgataatgct aaaaaaatat aaatcgtaac gatcgttaaa
      841 tctcaacggc tggatcttat gacgaccgtt agaaattgtg gttgtcgacg agtcagtaat
      901 aaacggcgtc aaagtggttg cagccggcac acacgagtcg tgtttatcaa ctcaaagcac
      961 aaatactttt cctcaaccta aaaataaggc aattagccaa aaacaacttt gcgtgtaaac
     1021 aacgctcaat acacgtgtca ttttattatt agctattgct tcaccgcctt agctttctcg
     1081 tgacctagtc gtcctcgtct tttcttcttc ttcttctata aaacaatacc caaagagctc
     1141 ttcttcttca caattcagat ttcaatttct caaaatctta aaaactttct ctcaattctc
     1201 tctaccgtga tcaaggtaaa tttctgtgtt ccttattctc tcaaaatctt cgattttgtt
     1261 ttcgttcgat cccaatttcg tatatgttct ttggtttaga ttctgttaat cttagatcga
     1321 agacgatttt ctgggtttga tcgttagata tcatcttaat tctcgattag ggtttcatag
     1381 atatcatccg atttgttcaa ataatttgag ttttgtcgaa taattactct tcgatttgtg
     1441 atttctatct agatctggtg ttagtttcta gtttgtgcga tcgaatttgt cgattaatct
     1501 gagtttttct gattaacagg tacaagctta cgcgtcctcc ataactatgg gcaagggcga
     1561 ggaactgttc actggcgtgg tcccaatcct ggtggaactg gatggtgatg tgaacgggca
     1621 caagttctcc gtcagcggag agggtgaagg tgatgccacc tacggaaagc tcaccctgaa
     1681 gttcatctgc actaccggaa agctccctgt tccgtggcca accctcgtca ccactttcac
     1741 ctacggtgtt cagtgcttct cccggtaccc agatcacatg aagcagcatg acttcttcaa
     1801 gagcgccatg cccgaaggct acgtgcaaga aaggactatc ttcttcaagg atgacgggaa
     1861 ctacaagaca cgtgccgaag tcaagttcga aggtgatacc ctggtgaacc gcatcgagct
     1921 gaaaggtaag tttctgcttc tacctttgat atatatataa taattatcat taattagtag
     1981 taatataata tttcaaatat ttttttcaaa ataaaagaat gtagtatata gcaattgctt
     2041 ttctgtagtt tataagtgtg tatattttaa tttataactt ttctaatata tgaccaaaat
     2101 ttgttgatgt gcaggtatcg atttcaagga agatggaaac atcctcggac acaagctgga
     2161 gtacaactac aactcccaca acgtatacat catggccgac aagcagaaga acggcatcaa
     2221 ggtgaacttc aagatcaggc acaacatcga agatggaagc gtgcaactgg cggaccacta
     2281 ccagcagaac acgcccatcg gcgatggccc tgtcctgctg ccggacaacc attacctgtc
     2341 cacgcaatct gccctctcca aggaccccaa cgagaagagg gaccacatgg tcctgctgga
     2401 gttcgtgacg gctgctggga tcacgcatgg catggatgaa ctctacaagt gactcgtcga
     2461 ccctgcttta atgagatatg cgagacgcct atgatcgcat gatatttgct ttcaattctg
     2521 ttgtgcacgt tgtaaaaaac ctgagcatgt gtagctcaga tccttaccgc cggtttcggt
     2581 tcattctaat gaatatatca cccgttacta tcgtattttt atgaataata ttctccgttc
     2641 aatttactga ttgtcctcga caggccttaa gggccagatc ttgggcccgg tacccaattc
     2701 gccctatagt gagtcgtatt acaattcact ggccgtcgtt ttacaacgtc gtgactggga
     2761 aaaccctggc gttacccaac ttaatcgcct tgcagcacat ccccctttcg ccagctggcg
     2821 taatagcgaa gaggcccgca ccgatcgccc ttcccaacag ttgcgcagcc tgaatggcga
     2881 atggaaattg taagcgttaa tattttgtta aaattcgcgt taaatttttg ttaaatcagc
     2941 tcatttttta accaataggc cgaaatcggc aaaatccctt ataaatcaaa agaatagacc
     3001 gagatagggt tgagtgttgt tccagtttgg aacaagagtc cactattaaa gaacgtggac
     3061 tccaacgtca aagggcgaaa aaccgtctat cagggcgatg gcccactacg tgaaccatca
     3121 ccctaatcaa gttttttggg gtcgaggtgc cgtaaagcac taaatcggaa ccctaaaggg
     3181 agcccccgat ttagagcttg acggggaaag ccggcgaacg tggcgagaaa ggaagggaag
     3241 aaagcgaaag gagcgggcgc tagggcgctg gcaagtgtag cggtcacgct gcgcgtaacc
     3301 accacacccg ccgcgcttaa tgcgccgcta cagggcgcgt caggtggcac ttttcgggga
     3361 aatgtgcgcg gaacccctat ttgtttattt ttctaaatac attcaaatat gtatccgctc
     3421 atgagacaat aaccctgata aatgcttcaa taatattgaa aaaggaagag tatgagtatt
     3481 caacatttcc gtgtcgccct tattcccttt tttgcggcat tttgccttcc tgtttttgct
     3541 cacccagaaa cgctggtgaa agtaaaagat gctgaagatc agttgggtgc acgagtgggt
     3601 tacatcgaac tggatctcaa cagcggtaag atccttgaga gttttcgccc cgaagaacgt
     3661 tttccaatga tgagcacttt taaagttctg ctatgtggcg cggtattatc ccgtattgac
     3721 gccgggcaag agcaactcgg tcgccgcata cactattctc agaatgactt ggttgagtac
     3781 tcaccagtca cagaaaagca tcttacggat ggcatgacag taagagaatt atgcagtgct
     3841 gccataacca tgagtgataa cactgcggcc aacttacttc tgacaacgat cggaggaccg
     3901 aaggagctaa ccgctttttt gcacaacatg ggggatcatg taactcgcct tgatcgttgg
     3961 gaaccggagc tgaatgaagc cataccaaac gacgagcgtg acaccacgat gcctgtagca
     4021 atggcaacaa cgttgcgcaa actattaact ggcgaactac ttactctagc ttcccggcaa
     4081 caattaatag actggatgga ggcggataaa gttgcaggac cacttctgcg ctcggccctt
     4141 ccggctggct ggtttattgc tgataaatct ggagccggtg agcgtgggtc tcgcggtatc
     4201 attgcagcac tggggccaga tggtaagccc tcccgtatcg tagttatcta cacgacgggg
     4261 agtcaggcaa ctatggatga acgaaataga cagatcgctg agataggtgc ctcactgatt
     4321 aagcattggt aactgtcaga ccaagtttac tcatatatac tttagattga tttaaaactt
     4381 catttttaat ttaaaaggat ctaggtgaag atcctttttg ataatctcat gaccaaaatc
     4441 ccttaacgtg agttttcgtt ccactgagcg tcagaccccg tagaaaagat caaaggatct
     4501 tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta
     4561 ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc tttttccgaa ggtaactggc
     4621 ttcagcagag cgcagatacc aaatactgtc cttctagtgt agccgtagtt aggccaccac
     4681 ttcaagaact ctgtagcacc gcctacatac ctcgctctgc taatcctgtt accagtggct
     4741 gctgccagtg gcgataagtc gtgtcttacc gggttggact caagacgata gttaccggat
     4801 aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac agcccagctt ggagcgaacg
     4861 acctacaccg aactgagata cctacagcgt gagctatgag aaagcgccac gcttcccgaa
     4921 gggagaaagg cggacaggta tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg
     4981 gagcttccag ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg ccacctctga
     5041 cttgagcgtc gatttttgtg atgctcgtca ggggggcgga gcctatggaa aaacgccagc
     5101 aacgcggcct ttttacggtt cctggccttt tgctggcctt ttgctcacat gttctttcct
     5161 gcgttatccc ctgattctgt ggataaccgt attaccgcct ttgagtgagc tgataccgct
     5221 cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg aggaagcgga agagcgccca
     5281 atacgcaaac cgcctctccc cgcgcgttgg ccgattcatt aatgcagctg gcacgacagg
     5341 tttcccgact ggaaagcggg cagtgagcgc aacgcaatta atgtgagtta gctcactcat
     5401 taggcacccc aggctttaca ctttatgctt ccggctcgta tgttgtgtgg aattgtgagc
     5461 ggataacaat ttcacacagg aaacagctat gaccatgatt acgccaagct cgaaattaac
     5521 cctcactaaa gggaacaaaa gctg
//
