LOCUS B471p4oM-LH 10653 bp DNA circular SYN 22-JAN-2016 DEFINITION Ligation of AHAS-Xho-Bspe* into B474p7oM-LH* ACCESSION B471p4oM-LH KEYWORDS . SOURCE Unknown. ORGANISM Unknown Unclassified. REFERENCE 1 (bases 1 to 10653) AUTHORS Self JOURNAL Unpublished. COMMENT SECID/File created by Clone Manager, Scientific & Educational Software COMMENT SECNOTES|Vector molecule: B474p7oM-LH* Fragment ends: BspEI and XhoI Fragment size: 8433 Insert molecule: AHAS-Xho-Bspe* Fragment ends: XhoI and BspEI Fragment size: 2220 Primer for gene synthesis XhoAhas cccgtcctcgagtcctccaccgaaATGGCCGCTGCCA BspeAHAS CCCGTCtccggATTAGTACTTGATGCGTCCGT Sequencing primers RBCS-TR2 CACAGTAAATTACAAGCACAAC STLS GGTAAGTTTCTGCTTCTACCT 35S GCACAATCCCACTATCCTT FEATURES Location/Qualifiers misc_feature 1..24 /gene="LB" /product="Left Border" /SECDrawAs="Region" /SECStyleId=1 misc_feature 309..724 /gene="P35S" /product="35S-Promotor" /SECDrawAs="Region" /SECStyleId=1 CDS 745..1383 /gene="ahas" /translation="MAAATTTTTTSSSISFSTKPSPSSSKSPLPISRFSLPFSLNPNK SSSSSRRRGIKSSSPSSISAVLNTTTNVTTTPSPTKPTKPETFISRFAPDQPRKGADI LVEALERQGVETVFAYPGGASMEIHQALTRSSSIRNVLPRHEQGGVFAAEGYARSSGK PGICIATSGPGATNLVSGLADALLDSVPLVAITGQVPRRMIGTDAFQETPIVE" /SECDrawAs="Gene" /SECStyleId=1 /SECName="ahas" CDS 1384..1572 /gene="int" /product="intron StLS1" /SECDrawAs="Gene" /SECStyleId=1 CDS 1573..2946 /gene="ahas" /translation="VTRSITKHNYLVMDVEDIPRIIEEAFFLATSGRPGPVLVDVPKD IQQQLAIPNWEQAMRLPGYMSRMPKPPEDSHLEQIVRLISESKKPVLYVGGGCLNSSD ELGRFVELTGIPVASTLMGLGSYPCDDELSLHMLGMHGTVYANYAVEHSDLLLAFGVR FDDRVTGKLEAFASRAKIVHIDIDSAEIGKNKTPHVSVCGDVKLALQGMNKVLENRAE ELKLDFGVWRNELNVQKQKFPLSFKTFGEAIPPQYAIKVLDELTDGKAIISTGVGQHQ MWAAQFYNYKKPRQWLSSGGLGAMGFGLPAAIGASVANPDAIVVDIDGDGSFIMNVQE LATIRVENLPVKVLLLNNQHLGMVMQWEDRFYKANRAHTFLGDPAQEDEIFPNMLLFA AACGIPAARVTKKADLREAIQTMLDTPGPYLLDVICPHQEHVLPMIPSGGTFNDVITE GDGRIKY" /SECDrawAs="Gene" /SECStyleId=1 misc_feature 2964..3254 /gene="TE9" /product="Terminator Rbcs-E9" /SECDrawAs="Region" /SECStyleId=1 misc_feature 3430..3453 /gene="RB" /product="Right Border" /SECDrawAs="Region" /SECStyleId=1 misc_feature 3988..4685 /gene="ColE1" /product="ColE1 ORI" /SECDrawAs="Region" /SECStyleId=1 misc_feature 5234..8939 /gene="pVS1" /product="origin of replication for agrobacteria" /SECDrawAs="Region" /SECStyleId=1 CDS 9123..10133 /gene="Sm/Sp" /product="Strep/Spec resistence" /SECDrawAs="Gene" /SECStyleId=1 ORIGIN 1 ggcaggatat attcaattgt aaatggcttc atgtccggga aatctacatg gatcagcaat 61 gagtatgatg gtcaatatgg agaaaaagaa agagtaatta ccaatttttt ttcaattcaa 121 aaatgtagat gtccgcagcg ttattataaa atgaaagtac attttgataa aacgacaaat 181 tacgatccgt cgtatttata ggcgaaagca ataaacaaat tattctaatt cggaaatctt 241 tatttcgacg tgtctacatt cacgtccaaa tgggggctta gatgagaaac ttcacgatcg 301 gctctagaac atggtggagc acgacactct cgtctactcc aagaatatca aagatacagt 361 ctcagaagac cagagggcta ttgagacttt tcaacaaagg gtaatatcgg gaaacctcct 421 cggattccat tgcccagcta tctgtcactt catcgaaagg acagtagaaa aggaagatgg 481 cttctacaaa tgccatcatt gcgataaagg aaaggctatc gttcaagatg cctctaccga 541 cagtggtccc aaagatggac ccccacccac gaggaacatc gtggaaaaag aagacgttcc 601 aaccacgtct tcaaagcaag tggattgatg tgatatctcc actgacgtaa gggatgacgc 661 acaatcccac tatccttcgc aagacccttc ctctatataa ggaagttcat ttcatttgga 721 gaggacctcg agtcctccac cgaaatggcc gctgccacca ccactaccac cacctccagc 781 tctatctcct tctcgaccaa gcccagccca tcctcaagca agtcccctct cccgatttcc 841 cgcttcagcc tgcccttctc tttgaaccca aacaagtcca gctcgtccag caggcgtcgc 901 ggcatcaagt cctctagccc ctcctcaatc tccgcagtgc ttaacactac caccaatgtc 961 accaccactc cttcgccgac caagccaacc aagcccgaga ccttcatcag cagatttgct 1021 cccgaccagc caaggaaggg cgccgacatt ctcgttgagg ccctggagcg ccaaggcgtg 1081 gaaaccgtct tcgcctaccc tggtggggcg tctatggaga tccaccaggc cctcactcgt 1141 tcctccagca tccgcaacgt gttgccgagg cacgagcagg gcggagtctt cgctgccgag 1201 ggctacgccc gctccagcgg aaaacccggc atctgcattg ctacctcggg tccaggcgcc 1261 accaaccttg tttcaggact cgcagatgcc ctgctcgact ccgtgccctt ggtcgctatc 1321 accggccaag ttcctcgtag aatgatcggg accgacgcct tccaagagac cccgatcgtc 1381 gaggtaagtt tctgcttcta cctttgatat atatataata attatcatta attagtagta 1441 atataatatt tcaaatattt ttttcaaaat aaaagaatgt agtatatagc aattgctttt 1501 ctgtagttta taagtgtgta tattttaatt tataactttt ctaatatatg accaaaattt 1561 gttgatgtgc aggttactcg cagcattacc aagcacaact accttgtgat ggacgtggaa 1621 gacatcccaa ggatcatcga ggaggccttc ttcctggcga cctctggccg tcccggacct 1681 gtcctcgtcg atgtgcccaa ggacattcag cagcaactcg ccatcccaaa ctgggagcag 1741 gctatgcgcc tgccgggcta catgtccagg atgcccaagc cacctgagga ctcccatttg 1801 gagcagatcg tgcgccttat cagcgaatcc aagaagcccg tcctctacgt gggaggtggc 1861 tgcctgaaca gctcggacga gcttgggaga ttcgtcgagt tgaccggcat tccggtggcc 1921 tccaccctca tgggactcgg ctcttaccca tgcgacgatg agctgagcct ccacatgttg 1981 ggtatgcacg gcactgttta cgccaattat gctgtcgagc actccgacct gcttctcgcc 2041 ttcggagtgc gttttgacga ccgcgtcacc ggcaagctgg aggcattcgc ctcaagggct 2101 aagatcgtgc acatcgacat tgattccgcc gaaatcggaa agaacaagac ccctcacgtc 2161 agcgtttgcg gcgacgtgaa gctcgccttg caggggatga acaaagttct tgagaaccgc 2221 gcggaggagc tgaagctcga cttcggcgtg tggcgtaacg agcttaacgt ccaaaagcag 2281 aagttcccct tgtccttcaa gaccttcgga gaggccatcc cgccccagta cgctatcaaa 2341 gtgctcgacg aactgaccga tggtaaggcc attatcagca ctggcgtcgg acagcaccaa 2401 atgtgggccg ctcagttcta caattacaag aagccacgcc agtggctctc gtccggcggg 2461 ctcggcgcaa tgggattcgg cctgccagcc gccatcggtg cttctgtcgc caacccagac 2521 gccatcgtgg tggacattga cggcgacggc agcttcatta tgaacgtcca agagttggct 2581 accatcaggg ttgagaacct tcctgtcaaa gttctcctgc tcaacaacca gcatttgggc 2641 atggtcatgc agtgggagga tagattttac aaggccaatc gcgcgcacac cttccttggg 2701 gacccggccc aggaggacga gattttcccc aacatgctcc tgttcgccgc tgcctgcggc 2761 atcccagcag cccgtgtgac caagaaggct gacctcaggg aagccatcca aaccatgttg 2821 gacactcccg gaccttacct tctggatgtg atctgcccgc accaggagca cgtcctccca 2881 atgatcccct ccggtggcac cttcaacgac gtgattaccg agggagacgg acgcatcaag 2941 tactaatccg gaatcgcgtt cgagtattat ggcattggga aaactgtttt tcttgtacca 3001 tttgttgtgc ttgtaattta ctgtgttttt tattcggttt tcgctatcga actgtgaaat 3061 ggaaatggat ggagaagagt taatgaatga tatggtcctt ttgttcattc tcaaattaat 3121 attatttgtt ttttctctta tttgttgtgt gttgaatttg aaattataag agatatgcaa 3181 acattttgtt ttgagtaaaa atgtgtcaaa tcgtggcctc taatgaccga agttaatatg 3241 aggagtaaaa cactgaagcc tgcaggcatg caagctgatc cactagaggc catggcggcc 3301 gcactagtgg atcccccggg atgcatgaat tcaagcttat cgatgtcgac aggccttaag 3361 ggccagatct tgggcccggt acccgatcag attgtcgttt cccgccttcg gtttaaacta 3421 tcagtgtttg acaggatata ttggcgggta aacctaagag aaaagagcgt ttattagaat 3481 aatcggatat ttaaaagggc gtgaaaaggt ttatccgttc gtccatttgt atgtgcatgc 3541 caaccacagg gttcccctcg ggagtgcttg gcattccgtg cgataatgac ttctgttcaa 3601 ccacccaaac gtcggaaagc ctgacgacgg agcagcattc caaaaagatc ccttggctcg 3661 tctgggtcgg ctagaaggtc gagtgggctg ctgtggcttg atccctcaac gcggtcgcgg 3721 acgtagcgca gcgccgaaaa atcctcgatc gcaaatccga cgctgtcgaa aagcgtgatc 3781 tgcttgtcgc tctttcggcc gacgtcctgg ccagtcatca cgcgccaaag ttccgtcaca 3841 ggatgatctg gcgcgagttg ctggatctcg ccttcaatcc gggtctgtgg cgggaactcc 3901 acgaaaatat ccgaacgcag caagatatcg cggtgcatct cggtcttgcc tgggcagtcg 3961 ccgccgacgc cgttgatgtg gacgccgaaa aggatctagg tgaagatcct ttttgataat 4021 ctcatgacca aaatccctta acgtgagttt tcgttccact gagcgtcaga ccccgtagaa 4081 aagatcaaag gatcttcttg agatcctttt tttctgcgcg taatctgctg cttgcaaaca 4141 aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc aagagctacc aactcttttt 4201 ccgaaggtaa ctggcttcag cagagcgcag ataccaaata ctgttcttct agtgtagccg 4261 tagttaggcc accacttcaa gaactctgta gcaccgccta catacctcgc tctgctaatc 4321 ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga 4381 cgatagttac cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc 4441 agcttggagc gaacgaccta caccgaactg agatacctac agcgtgagct atgagaaagc 4501 gccacgcttc ccgaagggag aaaggcggac aggtatccgg taagcggcag ggtcggaaca 4561 ggagagcgca cgagggagct tccaggggga aacgcctggt atctttatag tcctgtcggg 4621 tttcgccacc tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta 4681 tggaaaaacg ccagcaacgc ggccttttta cggttcctgg ccttttgctg gccttttgct 4741 cacatgttct ttcctgcgtt atcccctgat tctgtggata accgattacc gcctttgagt 4801 gagctgatac cgctcgccgc agccgaacga ccgagcgcag cgagtcagtg agcgaggaag 4861 cggaagagcg cctgatgcgg tattttctcc ttacgcatct gtgcggtatt tcacaccgca 4921 tatggtgcac tctcagtaca atctgctctg atgccgcata gttaagccag tatacactcc 4981 gctatcgcta cgtgactggg tcatggctgc gccccgacac ccgccaacac ccgctgacgc 5041 gccctgacgg gcttgtctgc tcccggcatc cgcttacaga caagctgtga ccgtctccgg 5101 gagctgcatg tgtcagaggt tttcaccgtc atcaccgaaa cgcgcgaggc aggggtacgt 5161 cgaggtcgat ccaacccctc cgctgctata gtgcagtcgg cttctgacgt tcagtgcagc 5221 cgtcttctga aaacgacatg tcgcacaagt cctaagttac gcgacaggct gccgccctgc 5281 ccttttcctg gcgttttctt gtcgcgtgtt ttagtcgcat aaagtagaat acttgcgact 5341 agaaccggag acattacgcc atgaacaaga gcgccgccgc tggcctgctg ggctatgccc 5401 gcgtcagcac cgacgaccag gacttgacca accaacgggc cgaactgcac gcggccggct 5461 gcaccaagct gttttccgag aagatcaccg gcaccaggcg cgaccgcccg gagctggcca 5521 ggatgcttga ccacctacgc cctggcgacg ttgtgacagt gaccaggcta gaccgcctgg 5581 cccgcagcac ccgcgaccta ctggacattg ccgagcgcat ccaggaggcc ggcgcgggcc 5641 tgcgtagcct ggcagagccg tgggccgaca ccaccacgcc ggccggccgc atggtgttga 5701 ccgtgttcgc cggcattgcc gagttcgagc gttccctaat catcgaccgc acccggagcg 5761 ggcgcgaggc cgccaaggcg cgaggcgtga agtttggccc ccgccctacc ctcaccccgg 5821 cacagatcgc gcacgcccgc gagctgatcg accaggaagg ccgcaccgtg aaagaggcgg 5881 ctgcactgct tggcgtgcat cgctcgaccc tgtaccgcgc acttgagcgc agcgaggaag 5941 tgacgcccac cgaggccagg cggcgcggtg ccttccgtga ggacgcattg accgaggccg 6001 acgccctggc ggccgccgag aatgaacgcc aagaggaaca agcatgaaac cgcaccagga 6061 cggccaggac gaaccgtttt tcattaccga agagatcgag gcggagatga tcgcggccgg 6121 gtacgtgttc gagccgcccg cgcacgtctc aaccgtgcgg ctgcatgaaa tcctggccgg 6181 tttgtctgat gccaagctcg cggcctggcc ggcgagcttg gccgctgaag aaaccgagcg 6241 ccgccgtcta aaaaggtgat gtgtatttga gtaaaacagc ttgcgtcatg cggtcgctgc 6301 gtatatgatg cgatgagtaa ataaacaaat acgcaagggg aacgcatgaa ggttatcgct 6361 gtacttaacc agaaaggcgg gtcaggcaag acgaccatcg caacccatct agcccgcgcc 6421 ctgcaactcg ccggggccga tgttctgtta gtcgattccg atccccaggg cagtgcccgc 6481 gattgggcgg ccgtgcggga agatcaaccg ctaaccgttg tcggcatcga ccgcccgacg 6541 attgaccgcg acgtgaaggc catcggccgg cgcgacttcg tagtgatcga cggagcgccc 6601 caggcggcgg acttggctgt gtccgcgatc aaggcagccg acttcgtgct gattccggtg 6661 cagccaagcc cttacgacat atgggccacc gccgacctgg tggagctggt taagcagcgc 6721 attgaggtca cggatggaag gctacaagcg gcctttgtcg tgtcgcgggc gatcaaaggc 6781 acgcgcatcg gcggtgaggt tgccgaggcg ctggccgggt acgagctgcc cattcttgag 6841 tcccgtatca cgcagcgcgt gagctaccca ggcactgccg ccgccggcac aaccgttctt 6901 gaatcagaac ccgagggcga cgctgcccgc gaggtccagg cgctggccgc tgaaattaaa 6961 tcaaaactca tttgagttaa tgaggtaaag agaaaatgag caaaagcaca aacacgctaa 7021 gtgccggccg tccgagcgca cgcagcagca aggctgcaac gttggccagc ctggcagaca 7081 cgccagccat gaagcgggtc aactttcagt tgccggcgga ggatcacacc aagctgaaga 7141 tgtacgcggt acgccaaggc aagaccatta ccgagctgct atctgaatac atcgcgcagc 7201 taccagagta aatgagcaaa tgaataaatg agtagatgaa ttttagcggc taaaggaggc 7261 ggcatggaaa atcaagaaca accaggcacc gacgccgtgg aatgccccat gtgtggagga 7321 acgggcggtt ggccaggcgt aagcggctgg gttgtctgcc ggccctgcaa tggcactgga 7381 acccccaagc ccgaggaatc ggcgtgagcg gtcgcaaacc atccggcccg gtacaaatcg 7441 gcgcggcgct gggtgatgac ctggtggaga agttgaaggc ggcgcaggcc gcccagcggc 7501 aacgcatcga ggcagaagca cgccccggtg aatcgtggca agcggccgct gatcgaatcc 7561 gcaaagaatc ccggcaaccg ccggcagccg gtgcgccgtc gattaggaag ccgcccaagg 7621 gcgacgagca accagatttt ttcgttccga tgctctatga cgtgggcacc cgcgatagtc 7681 gcagcatcat ggacgtggcc gttttccgtc tgtcgaagcg tgaccgacga gctggcgagg 7741 tgatccgcta cgagcttcca gacgggcacg tagaggtttc cgcagggccg gccggcatgg 7801 cgagtgtgtg ggattacgac ctggtactga tggcggtttc ccatctaacc gaatccatga 7861 accgataccg ggaagggaag ggagacaagc ccggccgcgt gttccgtcca cacgttgcgg 7921 acgtactcaa gttctgccgg cgagccgatg gcggaaagca gaaagacgac ctggtagaaa 7981 cctgcattcg gttaaacacc acgcacgttg ccatgcagcg tacgaagaag gccaagaacg 8041 gccgcctggt gacggtatcc gagggtgaag ccttgattag ccgctacaag atcgtaaaga 8101 gcgaaaccgg gcggccggag tacatcgaga tcgagctagc tgattggatg taccgcgaga 8161 tcacagaagg caagaacccg gacgtgctga cggttcaccc cgattacttt ttgatcgatc 8221 ccggcatcgg ccgttttctc taccgcctgg cacgccgcgc cgcaggcaag gcagaagcca 8281 gatggttgtt caagacgatc tacgaacgca gtggcagcgc cggagagttc aagaagttct 8341 gtttcaccgt gcgcaagctg atcgggtcaa atgacctgcc ggagtacgat ttgaaggagg 8401 aggcggggca ggctggcccg atcctagtca tgcgctaccg caacctgatc gagggcgaag 8461 catccgccgg ttcctaatgt acggagcaga tgctagggca aattgcccta gcaggggaaa 8521 aaggtcgaaa aggtctcttt cctgtggata gcacgtacat tgggaaccca aagccgtaca 8581 ttgggaaccg gaacccgtac attgggaacc caaagccgta cattgggaac cggtcacaca 8641 tgtaagtgac tgatataaaa gagaaaaaag gcgatttttc cgcctaaaac tctttaaaac 8701 ttattaaaac tcttaaaacc cgcctggcct gtgcataact gtctggccag cgcacagccg 8761 aagagctgca aaaagcgcct acccttcggt cgctgcgctc cctacgcccc gccgcttcgc 8821 gtcggcctat cgcggccgct ggccgctcaa aaatggctgg cctacggcca ggcaatctac 8881 cagggcgcgg acaagccgcg ccgtcgccac tcgaccgccg gcgcccacat caaggcaccg 8941 gtgggtatgc ctgacgatgc gtggagaccg aaaccttgcg ctcgttcgcc agccaggaca 9001 gaaatgcctc gacttcgctg ctgcccaagg ttgccgggtg acgcacaccg tggaaacgga 9061 tgaaggcacg aacccagtgg acataagcct gttcggttcg taagctgtaa tgcaagtagc 9121 gtatgcgctc acgcaactgg tccagaacct tgaccgaacg cagcggtggt aacggcgcag 9181 tggcggtttt catggcttgt tatgactgtt tttttggggt acagtctatg cctcgggcat 9241 ccaagcagca agcgcgttac gccgtgggtc gatgtttgat gttatggagc agcaacgatg 9301 ttacgcagca gggcagtcgc cctaaaacaa agttaaacat catgagggaa gcggtgatcg 9361 ccgaagtatc gactcaacta tcagaggtag ttggcgtcat cgagcgccat ctcgaaccga 9421 cgttgctggc cgtacatttg tacggctccg cagtggatgg cggcctgaag ccacacagtg 9481 atattgattt gctggttacg gtgaccgtaa ggcttgatga aacaacgcgg cgagctttga 9541 tcaacgacct tttggaaact tcggcttccc ctggagagag cgagattctc cgcgctgtag 9601 aagtcaccat tgttgtgcac gacgacatca ttccgtggcg ttatccagct aagcgcgaac 9661 tgcaatttgg agaatggcag cgcaatgaca ttcttgcagg tatcttcgag ccagccacga 9721 tcgacattga tctggctatc ttgctgacaa aagcaagaga acatagcgtt gccttggtag 9781 gtccagcggc ggaggaactc tttgatccgg ttcctgaaca ggatctattt gaggcgctaa 9841 atgaaacctt aacgctatgg aactcgccgc ccgactgggc tggcgatgag cgaaatgtag 9901 tgcttacgtt gtcccgcatt tggtacagcg cagtaaccgg caaaatcgcg ccgaaggatg 9961 tcgctgccga ctgggcaatg gagcgcctgc cggcccagta tcagcccgtc atacttgaag 10021 ctagacaggc ttatcttgga caagaagaag atcgcttggc ctcgcgcgca gatcagttgg 10081 aagaatttgt ccactacgtg aaaggcgaga tcaccaaggt agtcggcaaa taatgtctaa 10141 caattcgttc aagccgacgc cgcttcgcgg cgcggcttaa ctcaagcgtt agatgcacta 10201 agcacataat tgctcacagc caaactatca ggtcaagtct gcttttatta tttttaagcg 10261 tgcataataa gccctacaca aattgggaga tatatcatga aaggctggct ttttcttgtt 10321 atcgcaatag ttggcgaagt aatcgcaaca tagcttgctt ggtcgttccg cgtgaacgtc 10381 ggctcgattg tacctgcgtt caaatacttt gcgatcgtgt tgcgcgcctg cccggtgcgt 10441 cggctgatct cacggatcga ctgcttctct cgcaacgcca tccgacggat gatgtttaaa 10501 agtcccatgt ggatcactcc gttgccccgt cgctcaccgt gttgggggga aggtgcacat 10561 ggctcagttc tcaatggaaa ttatctgcct aaccggctca gttctgcgta gaaaccaaca 10621 tgcaagctcc accgggtgca aagcggcagc ggc //