LOCUS       B472p5oM-LH            10216 bp    DNA     circular SYN 22-JAN-2016
DEFINITION  B472p5oM-LH rotated to position 9969
ACCESSION   B472p5oM-LH
KEYWORDS    .
SOURCE      Unknown.
  ORGANISM  Unknown
            Unclassified.
REFERENCE   1  (bases 1 to 10216)
  AUTHORS   Self
  JOURNAL   Unpublished.
COMMENT     SECID/File created by Clone Manager, Scientific & Educational Software
COMMENT     SECNOTES|Vector molecule:  269p6i-TE9*
               Fragment ends: MluI and XhoI
               Fragment size: 8426
            Insert molecule:  EPSPS-Xho-Mlu*
               Fragment ends: XhoI and MluI
               Fragment size: 1790
            Primer for gene synthesis
            XhoEP	cccgtcctcgagtcctccaccgaaATGGCCCAGATCAAC
            MluEP	CCCGacgcgTTAGGCAGCCTTGGT
            Sequencing primers	
            35S	GCACAATCCCACTATCCTT    Maxi
            RBCS-TR2	CACAGTAAATTACAAGCACAAC
FEATURES             Location/Qualifiers
     misc_feature    1..24
                     /gene="LB"
                     /product="Left Border"
                     /SECDrawAs="Region"
                     /SECStyleId=1
     misc_feature    309..724
                     /gene="P35S"
                     /product="35S-Promotor"
                     /SECDrawAs="Region"
                     /SECStyleId=1
     CDS             745..1485
                     /gene="EPSPS"
                     /translation="MAQINNMAQGIQTLNPNSNFHKPQVPKSSSFLVFGSKKLKNSAN
                     SMLVLKKDSIFMQKFCSFRISASVATACMLHGASSRPATARKSSGLSGTVRIPGDKSI
                     SHRSFMFGGLASGETRITGLLEGEDVINTGKAMQAMGARIRKEGDTWIIDGVGNGGLL
                     APEAPLDFGNAATGCRLTMGLVGVYDFDSTFIGDASLTKRPMGRVLNPLREMGVQVKS
                     EDGDRLPVTLRGPKTPTPITYRVPMASAQ"
                     /SECDrawAs="Gene"
                     /SECStyleId=1
                     /SECName="epsps"
     misc_feature    1486..1674
                     /gene="int"
                     /product="Intron STLS1"
                     /SECDrawAs="Gene"
                     /SECStyleId=1
                     /SECName="int"
                     /SECDescr="Intron STLS1"
     CDS             1675..2517
                     /gene="epsps"
                     /codon_start=3
                     /SECDrawAs="Gene"
                     /SECStyleId=1
     misc_feature    2527..2817
                     /gene="TE9"
                     /product="Terminator Rbcs-E9"
                     /SECDrawAs="Region"
                     /SECStyleId=1
     misc_feature    2993..3016
                     /gene="RB"
                     /product="Right Border"
                     /SECDrawAs="Region"
                     /SECStyleId=1
     misc_feature    3551..4248
                     /gene="ColE1"
                     /product="ColE1 ORI"
                     /SECDrawAs="Region"
                     /SECStyleId=1
     misc_feature    4797..8502
                     /gene="pVS1"
                     /product="origin of replication for agrobacteria"
                     /SECDrawAs="Region"
                     /SECStyleId=1
     CDS             8686..9696
                     /gene="Sm/Sp"
                     /product="Strep/Spec resistence"
                     /SECDrawAs="Gene"
                     /SECStyleId=1
ORIGIN      
        1 ggcaggatat attcaattgt aaatggcttc atgtccggga aatctacatg gatcagcaat
       61 gagtatgatg gtcaatatgg agaaaaagaa agagtaatta ccaatttttt ttcaattcaa
      121 aaatgtagat gtccgcagcg ttattataaa atgaaagtac attttgataa aacgacaaat
      181 tacgatccgt cgtatttata ggcgaaagca ataaacaaat tattctaatt cggaaatctt
      241 tatttcgacg tgtctacatt cacgtccaaa tgggggctta gatgagaaac ttcacgatcg
      301 gctctagaac atggtggagc acgacactct cgtctactcc aagaatatca aagatacagt
      361 ctcagaagac cagagggcta ttgagacttt tcaacaaagg gtaatatcgg gaaacctcct
      421 cggattccat tgcccagcta tctgtcactt catcgaaagg acagtagaaa aggaagatgg
      481 cttctacaaa tgccatcatt gcgataaagg aaaggctatc gttcaagatg cctctaccga
      541 cagtggtccc aaagatggac ccccacccac gaggaacatc gtggaaaaag aagacgttcc
      601 aaccacgtct tcaaagcaag tggattgatg tgatatctcc actgacgtaa gggatgacgc
      661 acaatcccac tatccttcgc aagacccttc ctctatataa ggaagttcat ttcatttgga
      721 gaggacctcg agtcctccac cgaaatggcc cagatcaaca acatggctca gggcattcag
      781 accctcaacc ccaattccaa cttccacaag ccacaagtgc ctaagtctag ctccttcctc
      841 gtcttcggct cgaagaagtt gaagaacagc gccaactcca tgcttgttct caagaaagac
      901 tcaatcttca tgcagaagtt ttgcagcttc cgcatctccg caagtgtggc cactgcttgc
      961 atgttgcacg gtgcctccag ccgtccggcg accgccagga agtcttccgg cctctcgggg
     1021 accgtcagaa tccccggaga caagagcatt tcccaccgct cattcatgtt cggcggcctg
     1081 gccagcggag agacaaggat caccggcctt ctggagggtg aggatgtcat caacaccggg
     1141 aaggctatgc aggctatggg agcccgcatc cggaaggaag gcgacacgtg gattatcgac
     1201 ggcgtgggaa acggcggtct cctcgcccca gaggcaccct tggacttcgg caacgccgcc
     1261 accggatgcc gtctgactat ggggcttgtt ggcgtgtacg atttcgactc caccttcatc
     1321 ggagacgctt ccctcaccaa gcgccctatg ggcagggtcc taaatccgct ccgtgagatg
     1381 ggcgtgcaag tcaagagcga ggacggagac cgcctgccag ttaccttgcg aggccccaag
     1441 actccaacac ccatcaccta cagggtgcct atggcctctg cgcaggtaag tttctgcttc
     1501 tacctttgat atatatataa taattatcat taattagtag taatataata tttcaaatat
     1561 ttttttcaaa ataaaagaat gtagtatata gcaattgctt ttctgtagtt tataagtgtg
     1621 tatattttaa tttataactt ttctaatata tgaccaaaat ttgttgatgt gcaggtcaag
     1681 tccgccgtgc tccttgccgg gctgaacact ccgggtatta ccaccgtcat cgagcccatt
     1741 atgaccagag atcataccga gaagatgctc cagggattcg gcgctaactt gacggttgaa
     1801 accgacgccg acggcgtgcg caccatccgt ctggagggaa ggggcaaact caccggccaa
     1861 gtcatcgacg tgccaggaga tcccagctcg actgcttttc cacttgtagc cgccctcttg
     1921 gtccctggtt ccgacgtgac catcctgaac gttcttatga acccgacccg caccgggctc
     1981 atcttgactc tccaggagat gggcgctgac atcgaagtca ttaaccccag gctggccgga
     2041 ggcgaggacg tggcagattt gcgcgtccgt agttccaccc tgaagggtgt gacagttcca
     2101 gaggaccggg ctcccagcat gatcgacgag taccctatcc ttgccgtcgc cgcggccttc
     2161 gccgagggcg ctaccgtgat gaacggattg gaggagctgc gcgtcaagga gtcagacaga
     2221 ctctccgccg tggccaatgg gctcaagctc aacggcgttg actgcgatga gggagaaacc
     2281 agccttgtcg tgcgtggcag gccggacggc aagggactcg gtaacgcttc tggggccgca
     2341 gtcgccacgc acctggacca ccgcatcgcg atgtccttcc tcgtgatggg ccttgtttcg
     2401 gagaacccag tcaccgtgga cgatgccacc atgattgcta ctagcttccc cgagttcatg
     2461 gacttgatgg ccggactggg agccaagatc gagctgtccg acaccaaggc tgcctaacgc
     2521 gttcgagtat tatggcattg ggaaaactgt ttttcttgta ccatttgttg tgcttgtaat
     2581 ttactgtgtt ttttattcgg ttttcgctat cgaactgtga aatggaaatg gatggagaag
     2641 agttaatgaa tgatatggtc cttttgttca ttctcaaatt aatattattt gttttttctc
     2701 ttatttgttg tgtgttgaat ttgaaattat aagagatatg caaacatttt gttttgagta
     2761 aaaatgtgtc aaatcgtggc ctctaatgac cgaagttaat atgaggagta aaacactgaa
     2821 gcctgcaggc atgcaagctg atccactaga ggccatggcg gccgcactag tggatccccc
     2881 gggatgcatg aattcaagct tatcgatgtc gacaggcctt aagggccaga tcttgggccc
     2941 ggtacccgat cagattgtcg tttcccgcct tcggtttaaa ctatcagtgt ttgacaggat
     3001 atattggcgg gtaaacctaa gagaaaagag cgtttattag aataatcgga tatttaaaag
     3061 ggcgtgaaaa ggtttatccg ttcgtccatt tgtatgtgca tgccaaccac agggttcccc
     3121 tcgggagtgc ttggcattcc gtgcgataat gacttctgtt caaccaccca aacgtcggaa
     3181 agcctgacga cggagcagca ttccaaaaag atcccttggc tcgtctgggt cggctagaag
     3241 gtcgagtggg ctgctgtggc ttgatccctc aacgcggtcg cggacgtagc gcagcgccga
     3301 aaaatcctcg atcgcaaatc cgacgctgtc gaaaagcgtg atctgcttgt cgctctttcg
     3361 gccgacgtcc tggccagtca tcacgcgcca aagttccgtc acaggatgat ctggcgcgag
     3421 ttgctggatc tcgccttcaa tccgggtctg tggcgggaac tccacgaaaa tatccgaacg
     3481 cagcaagata tcgcggtgca tctcggtctt gcctgggcag tcgccgccga cgccgttgat
     3541 gtggacgccg aaaaggatct aggtgaagat cctttttgat aatctcatga ccaaaatccc
     3601 ttaacgtgag ttttcgttcc actgagcgtc agaccccgta gaaaagatca aaggatcttc
     3661 ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc
     3721 agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt
     3781 cagcagagcg cagataccaa atactgttct tctagtgtag ccgtagttag gccaccactt
     3841 caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc
     3901 tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa
     3961 ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac
     4021 ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg
     4081 gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga
     4141 gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact
     4201 tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa
     4261 cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc
     4321 gttatcccct gattctgtgg ataaccgatt accgcctttg agtgagctga taccgctcgc
     4381 cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg
     4441 cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatggtg cactctcagt
     4501 acaatctgct ctgatgccgc atagttaagc cagtatacac tccgctatcg ctacgtgact
     4561 gggtcatggc tgcgccccga cacccgccaa cacccgctga cgcgccctga cgggcttgtc
     4621 tgctcccggc atccgcttac agacaagctg tgaccgtctc cgggagctgc atgtgtcaga
     4681 ggttttcacc gtcatcaccg aaacgcgcga ggcaggggta cgtcgaggtc gatccaaccc
     4741 ctccgctgct atagtgcagt cggcttctga cgttcagtgc agccgtcttc tgaaaacgac
     4801 atgtcgcaca agtcctaagt tacgcgacag gctgccgccc tgcccttttc ctggcgtttt
     4861 cttgtcgcgt gttttagtcg cataaagtag aatacttgcg actagaaccg gagacattac
     4921 gccatgaaca agagcgccgc cgctggcctg ctgggctatg cccgcgtcag caccgacgac
     4981 caggacttga ccaaccaacg ggccgaactg cacgcggccg gctgcaccaa gctgttttcc
     5041 gagaagatca ccggcaccag gcgcgaccgc ccggagctgg ccaggatgct tgaccaccta
     5101 cgccctggcg acgttgtgac agtgaccagg ctagaccgcc tggcccgcag cacccgcgac
     5161 ctactggaca ttgccgagcg catccaggag gccggcgcgg gcctgcgtag cctggcagag
     5221 ccgtgggccg acaccaccac gccggccggc cgcatggtgt tgaccgtgtt cgccggcatt
     5281 gccgagttcg agcgttccct aatcatcgac cgcacccgga gcgggcgcga ggccgccaag
     5341 gcgcgaggcg tgaagtttgg cccccgccct accctcaccc cggcacagat cgcgcacgcc
     5401 cgcgagctga tcgaccagga aggccgcacc gtgaaagagg cggctgcact gcttggcgtg
     5461 catcgctcga ccctgtaccg cgcacttgag cgcagcgagg aagtgacgcc caccgaggcc
     5521 aggcggcgcg gtgccttccg tgaggacgca ttgaccgagg ccgacgccct ggcggccgcc
     5581 gagaatgaac gccaagagga acaagcatga aaccgcacca ggacggccag gacgaaccgt
     5641 ttttcattac cgaagagatc gaggcggaga tgatcgcggc cgggtacgtg ttcgagccgc
     5701 ccgcgcacgt ctcaaccgtg cggctgcatg aaatcctggc cggtttgtct gatgccaagc
     5761 tcgcggcctg gccggcgagc ttggccgctg aagaaaccga gcgccgccgt ctaaaaaggt
     5821 gatgtgtatt tgagtaaaac agcttgcgtc atgcggtcgc tgcgtatatg atgcgatgag
     5881 taaataaaca aatacgcaag gggaacgcat gaaggttatc gctgtactta accagaaagg
     5941 cgggtcaggc aagacgacca tcgcaaccca tctagcccgc gccctgcaac tcgccggggc
     6001 cgatgttctg ttagtcgatt ccgatcccca gggcagtgcc cgcgattggg cggccgtgcg
     6061 ggaagatcaa ccgctaaccg ttgtcggcat cgaccgcccg acgattgacc gcgacgtgaa
     6121 ggccatcggc cggcgcgact tcgtagtgat cgacggagcg ccccaggcgg cggacttggc
     6181 tgtgtccgcg atcaaggcag ccgacttcgt gctgattccg gtgcagccaa gcccttacga
     6241 catatgggcc accgccgacc tggtggagct ggttaagcag cgcattgagg tcacggatgg
     6301 aaggctacaa gcggcctttg tcgtgtcgcg ggcgatcaaa ggcacgcgca tcggcggtga
     6361 ggttgccgag gcgctggccg ggtacgagct gcccattctt gagtcccgta tcacgcagcg
     6421 cgtgagctac ccaggcactg ccgccgccgg cacaaccgtt cttgaatcag aacccgaggg
     6481 cgacgctgcc cgcgaggtcc aggcgctggc cgctgaaatt aaatcaaaac tcatttgagt
     6541 taatgaggta aagagaaaat gagcaaaagc acaaacacgc taagtgccgg ccgtccgagc
     6601 gcacgcagca gcaaggctgc aacgttggcc agcctggcag acacgccagc catgaagcgg
     6661 gtcaactttc agttgccggc ggaggatcac accaagctga agatgtacgc ggtacgccaa
     6721 ggcaagacca ttaccgagct gctatctgaa tacatcgcgc agctaccaga gtaaatgagc
     6781 aaatgaataa atgagtagat gaattttagc ggctaaagga ggcggcatgg aaaatcaaga
     6841 acaaccaggc accgacgccg tggaatgccc catgtgtgga ggaacgggcg gttggccagg
     6901 cgtaagcggc tgggttgtct gccggccctg caatggcact ggaaccccca agcccgagga
     6961 atcggcgtga gcggtcgcaa accatccggc ccggtacaaa tcggcgcggc gctgggtgat
     7021 gacctggtgg agaagttgaa ggcggcgcag gccgcccagc ggcaacgcat cgaggcagaa
     7081 gcacgccccg gtgaatcgtg gcaagcggcc gctgatcgaa tccgcaaaga atcccggcaa
     7141 ccgccggcag ccggtgcgcc gtcgattagg aagccgccca agggcgacga gcaaccagat
     7201 tttttcgttc cgatgctcta tgacgtgggc acccgcgata gtcgcagcat catggacgtg
     7261 gccgttttcc gtctgtcgaa gcgtgaccga cgagctggcg aggtgatccg ctacgagctt
     7321 ccagacgggc acgtagaggt ttccgcaggg ccggccggca tggcgagtgt gtgggattac
     7381 gacctggtac tgatggcggt ttcccatcta accgaatcca tgaaccgata ccgggaaggg
     7441 aagggagaca agcccggccg cgtgttccgt ccacacgttg cggacgtact caagttctgc
     7501 cggcgagccg atggcggaaa gcagaaagac gacctggtag aaacctgcat tcggttaaac
     7561 accacgcacg ttgccatgca gcgtacgaag aaggccaaga acggccgcct ggtgacggta
     7621 tccgagggtg aagccttgat tagccgctac aagatcgtaa agagcgaaac cgggcggccg
     7681 gagtacatcg agatcgagct agctgattgg atgtaccgcg agatcacaga aggcaagaac
     7741 ccggacgtgc tgacggttca ccccgattac tttttgatcg atcccggcat cggccgtttt
     7801 ctctaccgcc tggcacgccg cgccgcaggc aaggcagaag ccagatggtt gttcaagacg
     7861 atctacgaac gcagtggcag cgccggagag ttcaagaagt tctgtttcac cgtgcgcaag
     7921 ctgatcgggt caaatgacct gccggagtac gatttgaagg aggaggcggg gcaggctggc
     7981 ccgatcctag tcatgcgcta ccgcaacctg atcgagggcg aagcatccgc cggttcctaa
     8041 tgtacggagc agatgctagg gcaaattgcc ctagcagggg aaaaaggtcg aaaaggtctc
     8101 tttcctgtgg atagcacgta cattgggaac ccaaagccgt acattgggaa ccggaacccg
     8161 tacattggga acccaaagcc gtacattggg aaccggtcac acatgtaagt gactgatata
     8221 aaagagaaaa aaggcgattt ttccgcctaa aactctttaa aacttattaa aactcttaaa
     8281 acccgcctgg cctgtgcata actgtctggc cagcgcacag ccgaagagct gcaaaaagcg
     8341 cctacccttc ggtcgctgcg ctccctacgc cccgccgctt cgcgtcggcc tatcgcggcc
     8401 gctggccgct caaaaatggc tggcctacgg ccaggcaatc taccagggcg cggacaagcc
     8461 gcgccgtcgc cactcgaccg ccggcgccca catcaaggca ccggtgggta tgcctgacga
     8521 tgcgtggaga ccgaaacctt gcgctcgttc gccagccagg acagaaatgc ctcgacttcg
     8581 ctgctgccca aggttgccgg gtgacgcaca ccgtggaaac ggatgaaggc acgaacccag
     8641 tggacataag cctgttcggt tcgtaagctg taatgcaagt agcgtatgcg ctcacgcaac
     8701 tggtccagaa ccttgaccga acgcagcggt ggtaacggcg cagtggcggt tttcatggct
     8761 tgttatgact gtttttttgg ggtacagtct atgcctcggg catccaagca gcaagcgcgt
     8821 tacgccgtgg gtcgatgttt gatgttatgg agcagcaacg atgttacgca gcagggcagt
     8881 cgccctaaaa caaagttaaa catcatgagg gaagcggtga tcgccgaagt atcgactcaa
     8941 ctatcagagg tagttggcgt catcgagcgc catctcgaac cgacgttgct ggccgtacat
     9001 ttgtacggct ccgcagtgga tggcggcctg aagccacaca gtgatattga tttgctggtt
     9061 acggtgaccg taaggcttga tgaaacaacg cggcgagctt tgatcaacga ccttttggaa
     9121 acttcggctt cccctggaga gagcgagatt ctccgcgctg tagaagtcac cattgttgtg
     9181 cacgacgaca tcattccgtg gcgttatcca gctaagcgcg aactgcaatt tggagaatgg
     9241 cagcgcaatg acattcttgc aggtatcttc gagccagcca cgatcgacat tgatctggct
     9301 atcttgctga caaaagcaag agaacatagc gttgccttgg taggtccagc ggcggaggaa
     9361 ctctttgatc cggttcctga acaggatcta tttgaggcgc taaatgaaac cttaacgcta
     9421 tggaactcgc cgcccgactg ggctggcgat gagcgaaatg tagtgcttac gttgtcccgc
     9481 atttggtaca gcgcagtaac cggcaaaatc gcgccgaagg atgtcgctgc cgactgggca
     9541 atggagcgcc tgccggccca gtatcagccc gtcatacttg aagctagaca ggcttatctt
     9601 ggacaagaag aagatcgctt ggcctcgcgc gcagatcagt tggaagaatt tgtccactac
     9661 gtgaaaggcg agatcaccaa ggtagtcggc aaataatgtc taacaattcg ttcaagccga
     9721 cgccgcttcg cggcgcggct taactcaagc gttagatgca ctaagcacat aattgctcac
     9781 agccaaacta tcaggtcaag tctgctttta ttatttttaa gcgtgcataa taagccctac
     9841 acaaattggg agatatatca tgaaaggctg gctttttctt gttatcgcaa tagttggcga
     9901 agtaatcgca acatagcttg cttggtcgtt ccgcgtgaac gtcggctcga ttgtacctgc
     9961 gttcaaatac tttgcgatcg tgttgcgcgc ctgcccggtg cgtcggctga tctcacggat
    10021 cgactgcttc tctcgcaacg ccatccgacg gatgatgttt aaaagtccca tgtggatcac
    10081 tccgttgccc cgtcgctcac cgtgttgggg ggaaggtgca catggctcag ttctcaatgg
    10141 aaattatctg cctaaccggc tcagttctgc gtagaaacca acatgcaagc tccaccgggt
    10201 gcaaagcggc agcggc
//
