LOCUS       B473p6oM-LH             9666 bp    DNA     circular SYN 22-JAN-2016
DEFINITION  B473p6oM-LH rotated to position 9419
ACCESSION   B473p6oM-LH
KEYWORDS    .
SOURCE      Unknown.
  ORGANISM  Unknown
            Unclassified.
REFERENCE   1  (bases 1 to 9666)
  AUTHORS   Self
  JOURNAL   Unpublished.
COMMENT     SECID/File created by Clone Manager, Scientific & Educational Software
COMMENT     SECNOTES|Vector molecule:  B474p7oM-LH*
               Fragment ends: BspEI and XhoI
               Fragment size: 8433
            Insert molecule:  hpt-xho-Bspe*
               Fragment ends: XhoI and BspEI
               Fragment size: 1233
            Primer for gene synthesis
            XhoHpt	cccgtcctcgagtcctccaccgaaATGAAGAAACCGGAGC
            BspeHpt	CCCGTCtccggaTCACTCCTTGGCC
            Sequencing primers	
            LHX1	CTTTATTTCGACGTGTCTAC		Maxi only
            35S	GCACAATCCCACTATCCTT
            RBCS-TR2	CACAGTAAATTACAAGCACAAC
FEATURES             Location/Qualifiers
     misc_feature    1..24
                     /gene="LB"
                     /product="Left Border"
                     /SECDrawAs="Region"
                     /SECStyleId=1
     misc_feature    309..724
                     /gene="P35S"
                     /product="35S-Promotor"
                     /SECDrawAs="Region"
                     /SECStyleId=1
     CDS             745..1314
                     /gene="hptII"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGR
                     GYVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQD
                     LPETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVY
                     HWQTVMDDTVSASVAQALDELMLWAEDCPE"
                     /SECDrawAs="Gene"
                     /SECStyleId=1
                     /SECName="hptII"
     misc_feature    1315..1503
                     /gene="int"
                     /product="Intron StLS1"
                     /SECDrawAs="Gene"
                     /SECStyleId=1
                     /SECName="int"
                     /SECDescr="Intron StLS1"
     CDS             1504..1959
                     /gene="hptII"
                     /codon_start=3
                     /SECDrawAs="Gene"
                     /SECStyleId=1
     misc_feature    1977..2267
                     /gene="TE9"
                     /product="Terminator Rbcs-E9"
                     /SECDrawAs="Region"
                     /SECStyleId=1
     misc_feature    2443..2466
                     /gene="RB"
                     /product="Right Border"
                     /SECDrawAs="Region"
                     /SECStyleId=1
     misc_feature    3001..3698
                     /gene="ColE1"
                     /product="ColE1 ORI"
                     /SECDrawAs="Region"
                     /SECStyleId=1
     misc_feature    4247..7952
                     /gene="pVS1"
                     /product="origin of replication for agrobacteria"
                     /SECDrawAs="Region"
                     /SECStyleId=1
     CDS             8136..9146
                     /gene="Sm/Sp"
                     /product="Strep/Spec resistence"
                     /SECDrawAs="Gene"
                     /SECStyleId=1
ORIGIN      
        1 ggcaggatat attcaattgt aaatggcttc atgtccggga aatctacatg gatcagcaat
       61 gagtatgatg gtcaatatgg agaaaaagaa agagtaatta ccaatttttt ttcaattcaa
      121 aaatgtagat gtccgcagcg ttattataaa atgaaagtac attttgataa aacgacaaat
      181 tacgatccgt cgtatttata ggcgaaagca ataaacaaat tattctaatt cggaaatctt
      241 tatttcgacg tgtctacatt cacgtccaaa tgggggctta gatgagaaac ttcacgatcg
      301 gctctagaac atggtggagc acgacactct cgtctactcc aagaatatca aagatacagt
      361 ctcagaagac cagagggcta ttgagacttt tcaacaaagg gtaatatcgg gaaacctcct
      421 cggattccat tgcccagcta tctgtcactt catcgaaagg acagtagaaa aggaagatgg
      481 cttctacaaa tgccatcatt gcgataaagg aaaggctatc gttcaagatg cctctaccga
      541 cagtggtccc aaagatggac ccccacccac gaggaacatc gtggaaaaag aagacgttcc
      601 aaccacgtct tcaaagcaag tggattgatg tgatatctcc actgacgtaa gggatgacgc
      661 acaatcccac tatccttcgc aagacccttc ctctatataa ggaagttcat ttcatttgga
      721 gaggacctcg agtcctccac cgaaatgaag aaaccggagc tgaccgccac ttccgtcgaa
      781 aagttcctca tcgagaagtt tgatagcgtg tctgacctta tgcagttgtc ggagggcgaa
      841 gagtcccgcg cgttcagctt tgatgttggc gggcgcgggt acgtcctccg cgtgaactct
      901 tgcgctgacg gcttctataa agatcggtac gtttacaggc atttcgcctc ggcggccctg
      961 cctattcccg aagtcctcga catcggcgag ttttccgaga gccttacgta ttgtatttcc
     1021 cgccgggctc aaggggtgac cctccaggat ctgccggaaa ctgagttgcc tgcggttctc
     1081 cagcccgtcg ccgaggctat ggacgccatc gccgcggcgg atctttctca aacgtcgggc
     1141 ttcgggccgt tcggccctca ggggattggc cagtacacca cttggaggga ctttatctgc
     1201 gctatcgccg atccgcacgt gtatcattgg caaaccgtta tggacgatac ggtctccgcg
     1261 agcgtggccc aggctctgga cgaactcatg ttgtgggccg aggattgccc cgaggtaagt
     1321 ttctgcttct acctttgata tatatataat aattatcatt aattagtagt aatataatat
     1381 ttcaaatatt tttttcaaaa taaaagaatg tagtatatag caattgcttt tctgtagttt
     1441 ataagtgtgt atattttaat ttataacttt tctaatatat gaccaaaatt tgttgatgtg
     1501 caggtccgcc acctggttca tgcggacttc ggctctaata acgtgctcac cgataatggg
     1561 cggattactg ccgtcatcga ctggtccgaa gctatgtttg gcgattcgca gtacgaagtg
     1621 gcgaacattt tcttctggag gccttggctt gcctgcatgg aacagcaaac gcgctacttt
     1681 gagcggaggc acccggagct ggcggggtct ccccgcctca gggcctatat gttgcggatc
     1741 ggcctcgacc agctttacca gtccctggtt gatgggaatt tcgacgatgc tgcctgggcg
     1801 caaggccgct gtgacgctat tgtcaggagc ggcgcgggga ccgtgggccg cactcagatc
     1861 gcccggcggt cggctgccgt ttggacggat gggtgcgtcg aagtgctcgc ggacagcggc
     1921 aacaggcggc cttccacccg cccgcgggcc aaggagtgat ccggaatcgc gttcgagtat
     1981 tatggcattg ggaaaactgt ttttcttgta ccatttgttg tgcttgtaat ttactgtgtt
     2041 ttttattcgg ttttcgctat cgaactgtga aatggaaatg gatggagaag agttaatgaa
     2101 tgatatggtc cttttgttca ttctcaaatt aatattattt gttttttctc ttatttgttg
     2161 tgtgttgaat ttgaaattat aagagatatg caaacatttt gttttgagta aaaatgtgtc
     2221 aaatcgtggc ctctaatgac cgaagttaat atgaggagta aaacactgaa gcctgcaggc
     2281 atgcaagctg atccactaga ggccatggcg gccgcactag tggatccccc gggatgcatg
     2341 aattcaagct tatcgatgtc gacaggcctt aagggccaga tcttgggccc ggtacccgat
     2401 cagattgtcg tttcccgcct tcggtttaaa ctatcagtgt ttgacaggat atattggcgg
     2461 gtaaacctaa gagaaaagag cgtttattag aataatcgga tatttaaaag ggcgtgaaaa
     2521 ggtttatccg ttcgtccatt tgtatgtgca tgccaaccac agggttcccc tcgggagtgc
     2581 ttggcattcc gtgcgataat gacttctgtt caaccaccca aacgtcggaa agcctgacga
     2641 cggagcagca ttccaaaaag atcccttggc tcgtctgggt cggctagaag gtcgagtggg
     2701 ctgctgtggc ttgatccctc aacgcggtcg cggacgtagc gcagcgccga aaaatcctcg
     2761 atcgcaaatc cgacgctgtc gaaaagcgtg atctgcttgt cgctctttcg gccgacgtcc
     2821 tggccagtca tcacgcgcca aagttccgtc acaggatgat ctggcgcgag ttgctggatc
     2881 tcgccttcaa tccgggtctg tggcgggaac tccacgaaaa tatccgaacg cagcaagata
     2941 tcgcggtgca tctcggtctt gcctgggcag tcgccgccga cgccgttgat gtggacgccg
     3001 aaaaggatct aggtgaagat cctttttgat aatctcatga ccaaaatccc ttaacgtgag
     3061 ttttcgttcc actgagcgtc agaccccgta gaaaagatca aaggatcttc ttgagatcct
     3121 ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt
     3181 tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt cagcagagcg
     3241 cagataccaa atactgttct tctagtgtag ccgtagttag gccaccactt caagaactct
     3301 gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc tgccagtggc
     3361 gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa ggcgcagcgg
     3421 tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac ctacaccgaa
     3481 ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg
     3541 gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga gcttccaggg
     3601 ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact tgagcgtcga
     3661 tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa cgcggccttt
     3721 ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc gttatcccct
     3781 gattctgtgg ataaccgatt accgcctttg agtgagctga taccgctcgc cgcagccgaa
     3841 cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc
     3901 tccttacgca tctgtgcggt atttcacacc gcatatggtg cactctcagt acaatctgct
     3961 ctgatgccgc atagttaagc cagtatacac tccgctatcg ctacgtgact gggtcatggc
     4021 tgcgccccga cacccgccaa cacccgctga cgcgccctga cgggcttgtc tgctcccggc
     4081 atccgcttac agacaagctg tgaccgtctc cgggagctgc atgtgtcaga ggttttcacc
     4141 gtcatcaccg aaacgcgcga ggcaggggta cgtcgaggtc gatccaaccc ctccgctgct
     4201 atagtgcagt cggcttctga cgttcagtgc agccgtcttc tgaaaacgac atgtcgcaca
     4261 agtcctaagt tacgcgacag gctgccgccc tgcccttttc ctggcgtttt cttgtcgcgt
     4321 gttttagtcg cataaagtag aatacttgcg actagaaccg gagacattac gccatgaaca
     4381 agagcgccgc cgctggcctg ctgggctatg cccgcgtcag caccgacgac caggacttga
     4441 ccaaccaacg ggccgaactg cacgcggccg gctgcaccaa gctgttttcc gagaagatca
     4501 ccggcaccag gcgcgaccgc ccggagctgg ccaggatgct tgaccaccta cgccctggcg
     4561 acgttgtgac agtgaccagg ctagaccgcc tggcccgcag cacccgcgac ctactggaca
     4621 ttgccgagcg catccaggag gccggcgcgg gcctgcgtag cctggcagag ccgtgggccg
     4681 acaccaccac gccggccggc cgcatggtgt tgaccgtgtt cgccggcatt gccgagttcg
     4741 agcgttccct aatcatcgac cgcacccgga gcgggcgcga ggccgccaag gcgcgaggcg
     4801 tgaagtttgg cccccgccct accctcaccc cggcacagat cgcgcacgcc cgcgagctga
     4861 tcgaccagga aggccgcacc gtgaaagagg cggctgcact gcttggcgtg catcgctcga
     4921 ccctgtaccg cgcacttgag cgcagcgagg aagtgacgcc caccgaggcc aggcggcgcg
     4981 gtgccttccg tgaggacgca ttgaccgagg ccgacgccct ggcggccgcc gagaatgaac
     5041 gccaagagga acaagcatga aaccgcacca ggacggccag gacgaaccgt ttttcattac
     5101 cgaagagatc gaggcggaga tgatcgcggc cgggtacgtg ttcgagccgc ccgcgcacgt
     5161 ctcaaccgtg cggctgcatg aaatcctggc cggtttgtct gatgccaagc tcgcggcctg
     5221 gccggcgagc ttggccgctg aagaaaccga gcgccgccgt ctaaaaaggt gatgtgtatt
     5281 tgagtaaaac agcttgcgtc atgcggtcgc tgcgtatatg atgcgatgag taaataaaca
     5341 aatacgcaag gggaacgcat gaaggttatc gctgtactta accagaaagg cgggtcaggc
     5401 aagacgacca tcgcaaccca tctagcccgc gccctgcaac tcgccggggc cgatgttctg
     5461 ttagtcgatt ccgatcccca gggcagtgcc cgcgattggg cggccgtgcg ggaagatcaa
     5521 ccgctaaccg ttgtcggcat cgaccgcccg acgattgacc gcgacgtgaa ggccatcggc
     5581 cggcgcgact tcgtagtgat cgacggagcg ccccaggcgg cggacttggc tgtgtccgcg
     5641 atcaaggcag ccgacttcgt gctgattccg gtgcagccaa gcccttacga catatgggcc
     5701 accgccgacc tggtggagct ggttaagcag cgcattgagg tcacggatgg aaggctacaa
     5761 gcggcctttg tcgtgtcgcg ggcgatcaaa ggcacgcgca tcggcggtga ggttgccgag
     5821 gcgctggccg ggtacgagct gcccattctt gagtcccgta tcacgcagcg cgtgagctac
     5881 ccaggcactg ccgccgccgg cacaaccgtt cttgaatcag aacccgaggg cgacgctgcc
     5941 cgcgaggtcc aggcgctggc cgctgaaatt aaatcaaaac tcatttgagt taatgaggta
     6001 aagagaaaat gagcaaaagc acaaacacgc taagtgccgg ccgtccgagc gcacgcagca
     6061 gcaaggctgc aacgttggcc agcctggcag acacgccagc catgaagcgg gtcaactttc
     6121 agttgccggc ggaggatcac accaagctga agatgtacgc ggtacgccaa ggcaagacca
     6181 ttaccgagct gctatctgaa tacatcgcgc agctaccaga gtaaatgagc aaatgaataa
     6241 atgagtagat gaattttagc ggctaaagga ggcggcatgg aaaatcaaga acaaccaggc
     6301 accgacgccg tggaatgccc catgtgtgga ggaacgggcg gttggccagg cgtaagcggc
     6361 tgggttgtct gccggccctg caatggcact ggaaccccca agcccgagga atcggcgtga
     6421 gcggtcgcaa accatccggc ccggtacaaa tcggcgcggc gctgggtgat gacctggtgg
     6481 agaagttgaa ggcggcgcag gccgcccagc ggcaacgcat cgaggcagaa gcacgccccg
     6541 gtgaatcgtg gcaagcggcc gctgatcgaa tccgcaaaga atcccggcaa ccgccggcag
     6601 ccggtgcgcc gtcgattagg aagccgccca agggcgacga gcaaccagat tttttcgttc
     6661 cgatgctcta tgacgtgggc acccgcgata gtcgcagcat catggacgtg gccgttttcc
     6721 gtctgtcgaa gcgtgaccga cgagctggcg aggtgatccg ctacgagctt ccagacgggc
     6781 acgtagaggt ttccgcaggg ccggccggca tggcgagtgt gtgggattac gacctggtac
     6841 tgatggcggt ttcccatcta accgaatcca tgaaccgata ccgggaaggg aagggagaca
     6901 agcccggccg cgtgttccgt ccacacgttg cggacgtact caagttctgc cggcgagccg
     6961 atggcggaaa gcagaaagac gacctggtag aaacctgcat tcggttaaac accacgcacg
     7021 ttgccatgca gcgtacgaag aaggccaaga acggccgcct ggtgacggta tccgagggtg
     7081 aagccttgat tagccgctac aagatcgtaa agagcgaaac cgggcggccg gagtacatcg
     7141 agatcgagct agctgattgg atgtaccgcg agatcacaga aggcaagaac ccggacgtgc
     7201 tgacggttca ccccgattac tttttgatcg atcccggcat cggccgtttt ctctaccgcc
     7261 tggcacgccg cgccgcaggc aaggcagaag ccagatggtt gttcaagacg atctacgaac
     7321 gcagtggcag cgccggagag ttcaagaagt tctgtttcac cgtgcgcaag ctgatcgggt
     7381 caaatgacct gccggagtac gatttgaagg aggaggcggg gcaggctggc ccgatcctag
     7441 tcatgcgcta ccgcaacctg atcgagggcg aagcatccgc cggttcctaa tgtacggagc
     7501 agatgctagg gcaaattgcc ctagcagggg aaaaaggtcg aaaaggtctc tttcctgtgg
     7561 atagcacgta cattgggaac ccaaagccgt acattgggaa ccggaacccg tacattggga
     7621 acccaaagcc gtacattggg aaccggtcac acatgtaagt gactgatata aaagagaaaa
     7681 aaggcgattt ttccgcctaa aactctttaa aacttattaa aactcttaaa acccgcctgg
     7741 cctgtgcata actgtctggc cagcgcacag ccgaagagct gcaaaaagcg cctacccttc
     7801 ggtcgctgcg ctccctacgc cccgccgctt cgcgtcggcc tatcgcggcc gctggccgct
     7861 caaaaatggc tggcctacgg ccaggcaatc taccagggcg cggacaagcc gcgccgtcgc
     7921 cactcgaccg ccggcgccca catcaaggca ccggtgggta tgcctgacga tgcgtggaga
     7981 ccgaaacctt gcgctcgttc gccagccagg acagaaatgc ctcgacttcg ctgctgccca
     8041 aggttgccgg gtgacgcaca ccgtggaaac ggatgaaggc acgaacccag tggacataag
     8101 cctgttcggt tcgtaagctg taatgcaagt agcgtatgcg ctcacgcaac tggtccagaa
     8161 ccttgaccga acgcagcggt ggtaacggcg cagtggcggt tttcatggct tgttatgact
     8221 gtttttttgg ggtacagtct atgcctcggg catccaagca gcaagcgcgt tacgccgtgg
     8281 gtcgatgttt gatgttatgg agcagcaacg atgttacgca gcagggcagt cgccctaaaa
     8341 caaagttaaa catcatgagg gaagcggtga tcgccgaagt atcgactcaa ctatcagagg
     8401 tagttggcgt catcgagcgc catctcgaac cgacgttgct ggccgtacat ttgtacggct
     8461 ccgcagtgga tggcggcctg aagccacaca gtgatattga tttgctggtt acggtgaccg
     8521 taaggcttga tgaaacaacg cggcgagctt tgatcaacga ccttttggaa acttcggctt
     8581 cccctggaga gagcgagatt ctccgcgctg tagaagtcac cattgttgtg cacgacgaca
     8641 tcattccgtg gcgttatcca gctaagcgcg aactgcaatt tggagaatgg cagcgcaatg
     8701 acattcttgc aggtatcttc gagccagcca cgatcgacat tgatctggct atcttgctga
     8761 caaaagcaag agaacatagc gttgccttgg taggtccagc ggcggaggaa ctctttgatc
     8821 cggttcctga acaggatcta tttgaggcgc taaatgaaac cttaacgcta tggaactcgc
     8881 cgcccgactg ggctggcgat gagcgaaatg tagtgcttac gttgtcccgc atttggtaca
     8941 gcgcagtaac cggcaaaatc gcgccgaagg atgtcgctgc cgactgggca atggagcgcc
     9001 tgccggccca gtatcagccc gtcatacttg aagctagaca ggcttatctt ggacaagaag
     9061 aagatcgctt ggcctcgcgc gcagatcagt tggaagaatt tgtccactac gtgaaaggcg
     9121 agatcaccaa ggtagtcggc aaataatgtc taacaattcg ttcaagccga cgccgcttcg
     9181 cggcgcggct taactcaagc gttagatgca ctaagcacat aattgctcac agccaaacta
     9241 tcaggtcaag tctgctttta ttatttttaa gcgtgcataa taagccctac acaaattggg
     9301 agatatatca tgaaaggctg gctttttctt gttatcgcaa tagttggcga agtaatcgca
     9361 acatagcttg cttggtcgtt ccgcgtgaac gtcggctcga ttgtacctgc gttcaaatac
     9421 tttgcgatcg tgttgcgcgc ctgcccggtg cgtcggctga tctcacggat cgactgcttc
     9481 tctcgcaacg ccatccgacg gatgatgttt aaaagtccca tgtggatcac tccgttgccc
     9541 cgtcgctcac cgtgttgggg ggaaggtgca catggctcag ttctcaatgg aaattatctg
     9601 cctaaccggc tcagttctgc gtagaaacca acatgcaagc tccaccgggt gcaaagcggc
     9661 agcggc
//
