LOCUS B475p9oM-LH 9444 bp DNA circular SYN 22-JAN-2016 DEFINITION B475p9oM-LH rotated to position 9197 ACCESSION B475p9oM-LH KEYWORDS . SOURCE Unknown. ORGANISM Unknown Unclassified. REFERENCE 1 (bases 1 to 9444) AUTHORS Self JOURNAL Unpublished. COMMENT SECID/File created by Clone Manager, Scientific & Educational Software COMMENT SECNOTES|Vector molecule: B474p7oM-LH* Fragment ends: BspEI and XhoI Fragment size: 8433 Insert molecule: Npt-Xho-Bspe* Fragment ends: XhoI and BspEI Fragment size: 1011 Primer for gene synthesis XhoNpt cccgtcctcgagtcctccaccgaaATGGGAGCATGGATTG BspeNpt CCCGTCtccggaTCAGAAGAACTCGTCAAG Sequencing primers LHX1 CTTTATTTCGACGTGTCTAC Maxi only 35S GCACAATCCCACTATCCTT RBCS-TR2 CACAGTAAATTACAAGCACAAC FEATURES Location/Qualifiers misc_feature 1..24 /gene="LB" /product="Left Border" /SECDrawAs="Region" /SECStyleId=1 misc_feature 309..724 /gene="P35S" /product="35S-Promotor" /SECDrawAs="Region" /SECStyleId=1 CDS 745..1043 /gene="nptIIo" /product="NptII optimiert" /SECDrawAs="Gene" /SECStyleId=1 CDS 1044..1230 /gene="int" /SECDrawAs="Gene" /SECStyleId=1 CDS 1231..1737 /gene="nptIIo" /product="NptII optimiert" /SECDrawAs="Gene" /SECStyleId=1 misc_feature 1755..2045 /gene="TE9" /product="Terminator Rbcs-E9" /SECDrawAs="Region" /SECStyleId=1 misc_feature 2221..2244 /gene="RB" /product="Right Border" /SECDrawAs="Region" /SECStyleId=1 misc_feature 2779..3476 /gene="ColE1" /product="ColE1 ORI" /SECDrawAs="Region" /SECStyleId=1 misc_feature 4025..7730 /gene="pVS1" /product="origin of replication for agrobacteria" /SECDrawAs="Region" /SECStyleId=1 CDS 7914..8924 /gene="Sm/Sp" /product="Strep/Spec resistence" /SECDrawAs="Gene" /SECStyleId=1 ORIGIN 1 ggcaggatat attcaattgt aaatggcttc atgtccggga aatctacatg gatcagcaat 61 gagtatgatg gtcaatatgg agaaaaagaa agagtaatta ccaatttttt ttcaattcaa 121 aaatgtagat gtccgcagcg ttattataaa atgaaagtac attttgataa aacgacaaat 181 tacgatccgt cgtatttata ggcgaaagca ataaacaaat tattctaatt cggaaatctt 241 tatttcgacg tgtctacatt cacgtccaaa tgggggctta gatgagaaac ttcacgatcg 301 gctctagaac atggtggagc acgacactct cgtctactcc aagaatatca aagatacagt 361 ctcagaagac cagagggcta ttgagacttt tcaacaaagg gtaatatcgg gaaacctcct 421 cggattccat tgcccagcta tctgtcactt catcgaaagg acagtagaaa aggaagatgg 481 cttctacaaa tgccatcatt gcgataaagg aaaggctatc gttcaagatg cctctaccga 541 cagtggtccc aaagatggac ccccacccac gaggaacatc gtggaaaaag aagacgttcc 601 aaccacgtct tcaaagcaag tggattgatg tgatatctcc actgacgtaa gggatgacgc 661 acaatcccac tatccttcgc aagacccttc ctctatataa ggaagttcat ttcatttgga 721 gaggacctcg agtcctccac cgaaatggga gcatggattg agcaggatgg cctccatgct 781 ggtagccccg ctgcttgggt ggagcgcctc tttggctacg actgggccca acagaccatt 841 gggtgcagtg atgccgctgt cttccgtttg agcgcccagg gcaggccagt tctcttcgtg 901 aagactgacc tctccggtgc cctcaacgag ttgcaggacg aagctgcccg cctctcatgg 961 cttgccacca ccggagtccc ttgcgctgcc gtgttggacg ttgtcacaga ggccggaaga 1021 gactggctgc tcttgggcga ggtaagtttc tgcttctacc tttgatatat atataataat 1081 tatcattaat tagtagtaat ataatatttc aaatattttt ttcaaaataa aagaatgtag 1141 tatatagcaa ttgcttttct gtagtttata agtgtgtata ttttaattta taacttttct 1201 aatatatgac caaaatttgt tgatgtgcag gtgccaggcc aagacctcct gagctcccac 1261 ctggccccag ccgagaaggt ctccatcatg gcagacgcca tgcgtcgcct ccacaccctg 1321 gacccagcca cctgcccctt cgaccaccag gccaagcaca ggatcgaacg tgcacgcact 1381 aggatggagg ccggccttgt ggaccaggat gaccttgacg aggagcacca gggccttgct 1441 cccgccgagc tgttcgcccg cctcaaggct agaatgcctg acggagagga cttggtggtc 1501 acccacgggg atgcctgcct ccccaacatc atggttgaga acggacgctt ctctggcttc 1561 atcgactgcg gtcgtctggg cgtggccgac cgctaccaag acatcgccct ggccaccagg 1621 gacatcgctg aggaacttgg aggggagtgg gccgatcgtt tcttggtcct ctacggcatt 1681 gctgcccccg actcccagag gatcgccttc taccgcctgc ttgacgagtt cttctgatcc 1741 ggaatcgcgt tcgagtatta tggcattggg aaaactgttt ttcttgtacc atttgttgtg 1801 cttgtaattt actgtgtttt ttattcggtt ttcgctatcg aactgtgaaa tggaaatgga 1861 tggagaagag ttaatgaatg atatggtcct tttgttcatt ctcaaattaa tattatttgt 1921 tttttctctt atttgttgtg tgttgaattt gaaattataa gagatatgca aacattttgt 1981 tttgagtaaa aatgtgtcaa atcgtggcct ctaatgaccg aagttaatat gaggagtaaa 2041 acactgaagc ctgcaggcat gcaagctgat ccactagagg ccatggcggc cgcactagtg 2101 gatcccccgg gatgcatgaa ttcaagctta tcgatgtcga caggccttaa gggccagatc 2161 ttgggcccgg tacccgatca gattgtcgtt tcccgccttc ggtttaaact atcagtgttt 2221 gacaggatat attggcgggt aaacctaaga gaaaagagcg tttattagaa taatcggata 2281 tttaaaaggg cgtgaaaagg tttatccgtt cgtccatttg tatgtgcatg ccaaccacag 2341 ggttcccctc gggagtgctt ggcattccgt gcgataatga cttctgttca accacccaaa 2401 cgtcggaaag cctgacgacg gagcagcatt ccaaaaagat cccttggctc gtctgggtcg 2461 gctagaaggt cgagtgggct gctgtggctt gatccctcaa cgcggtcgcg gacgtagcgc 2521 agcgccgaaa aatcctcgat cgcaaatccg acgctgtcga aaagcgtgat ctgcttgtcg 2581 ctctttcggc cgacgtcctg gccagtcatc acgcgccaaa gttccgtcac aggatgatct 2641 ggcgcgagtt gctggatctc gccttcaatc cgggtctgtg gcgggaactc cacgaaaata 2701 tccgaacgca gcaagatatc gcggtgcatc tcggtcttgc ctgggcagtc gccgccgacg 2761 ccgttgatgt ggacgccgaa aaggatctag gtgaagatcc tttttgataa tctcatgacc 2821 aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag accccgtaga aaagatcaaa 2881 ggatcttctt gagatccttt ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca 2941 ccgctaccag cggtggtttg tttgccggat caagagctac caactctttt tccgaaggta 3001 actggcttca gcagagcgca gataccaaat actgttcttc tagtgtagcc gtagttaggc 3061 caccacttca agaactctgt agcaccgcct acatacctcg ctctgctaat cctgttacca 3121 gtggctgctg ccagtggcga taagtcgtgt cttaccgggt tggactcaag acgatagtta 3181 ccggataagg cgcagcggtc gggctgaacg gggggttcgt gcacacagcc cagcttggag 3241 cgaacgacct acaccgaact gagataccta cagcgtgagc tatgagaaag cgccacgctt 3301 cccgaaggga gaaaggcgga caggtatccg gtaagcggca gggtcggaac aggagagcgc 3361 acgagggagc ttccaggggg aaacgcctgg tatctttata gtcctgtcgg gtttcgccac 3421 ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac 3481 gccagcaacg cggccttttt acggttcctg gccttttgct ggccttttgc tcacatgttc 3541 tttcctgcgt tatcccctga ttctgtggat aaccgattac cgcctttgag tgagctgata 3601 ccgctcgccg cagccgaacg accgagcgca gcgagtcagt gagcgaggaa gcggaagagc 3661 gcctgatgcg gtattttctc cttacgcatc tgtgcggtat ttcacaccgc atatggtgca 3721 ctctcagtac aatctgctct gatgccgcat agttaagcca gtatacactc cgctatcgct 3781 acgtgactgg gtcatggctg cgccccgaca cccgccaaca cccgctgacg cgccctgacg 3841 ggcttgtctg ctcccggcat ccgcttacag acaagctgtg accgtctccg ggagctgcat 3901 gtgtcagagg ttttcaccgt catcaccgaa acgcgcgagg caggggtacg tcgaggtcga 3961 tccaacccct ccgctgctat agtgcagtcg gcttctgacg ttcagtgcag ccgtcttctg 4021 aaaacgacat gtcgcacaag tcctaagtta cgcgacaggc tgccgccctg cccttttcct 4081 ggcgttttct tgtcgcgtgt tttagtcgca taaagtagaa tacttgcgac tagaaccgga 4141 gacattacgc catgaacaag agcgccgccg ctggcctgct gggctatgcc cgcgtcagca 4201 ccgacgacca ggacttgacc aaccaacggg ccgaactgca cgcggccggc tgcaccaagc 4261 tgttttccga gaagatcacc ggcaccaggc gcgaccgccc ggagctggcc aggatgcttg 4321 accacctacg ccctggcgac gttgtgacag tgaccaggct agaccgcctg gcccgcagca 4381 cccgcgacct actggacatt gccgagcgca tccaggaggc cggcgcgggc ctgcgtagcc 4441 tggcagagcc gtgggccgac accaccacgc cggccggccg catggtgttg accgtgttcg 4501 ccggcattgc cgagttcgag cgttccctaa tcatcgaccg cacccggagc gggcgcgagg 4561 ccgccaaggc gcgaggcgtg aagtttggcc cccgccctac cctcaccccg gcacagatcg 4621 cgcacgcccg cgagctgatc gaccaggaag gccgcaccgt gaaagaggcg gctgcactgc 4681 ttggcgtgca tcgctcgacc ctgtaccgcg cacttgagcg cagcgaggaa gtgacgccca 4741 ccgaggccag gcggcgcggt gccttccgtg aggacgcatt gaccgaggcc gacgccctgg 4801 cggccgccga gaatgaacgc caagaggaac aagcatgaaa ccgcaccagg acggccagga 4861 cgaaccgttt ttcattaccg aagagatcga ggcggagatg atcgcggccg ggtacgtgtt 4921 cgagccgccc gcgcacgtct caaccgtgcg gctgcatgaa atcctggccg gtttgtctga 4981 tgccaagctc gcggcctggc cggcgagctt ggccgctgaa gaaaccgagc gccgccgtct 5041 aaaaaggtga tgtgtatttg agtaaaacag cttgcgtcat gcggtcgctg cgtatatgat 5101 gcgatgagta aataaacaaa tacgcaaggg gaacgcatga aggttatcgc tgtacttaac 5161 cagaaaggcg ggtcaggcaa gacgaccatc gcaacccatc tagcccgcgc cctgcaactc 5221 gccggggccg atgttctgtt agtcgattcc gatccccagg gcagtgcccg cgattgggcg 5281 gccgtgcggg aagatcaacc gctaaccgtt gtcggcatcg accgcccgac gattgaccgc 5341 gacgtgaagg ccatcggccg gcgcgacttc gtagtgatcg acggagcgcc ccaggcggcg 5401 gacttggctg tgtccgcgat caaggcagcc gacttcgtgc tgattccggt gcagccaagc 5461 ccttacgaca tatgggccac cgccgacctg gtggagctgg ttaagcagcg cattgaggtc 5521 acggatggaa ggctacaagc ggcctttgtc gtgtcgcggg cgatcaaagg cacgcgcatc 5581 ggcggtgagg ttgccgaggc gctggccggg tacgagctgc ccattcttga gtcccgtatc 5641 acgcagcgcg tgagctaccc aggcactgcc gccgccggca caaccgttct tgaatcagaa 5701 cccgagggcg acgctgcccg cgaggtccag gcgctggccg ctgaaattaa atcaaaactc 5761 atttgagtta atgaggtaaa gagaaaatga gcaaaagcac aaacacgcta agtgccggcc 5821 gtccgagcgc acgcagcagc aaggctgcaa cgttggccag cctggcagac acgccagcca 5881 tgaagcgggt caactttcag ttgccggcgg aggatcacac caagctgaag atgtacgcgg 5941 tacgccaagg caagaccatt accgagctgc tatctgaata catcgcgcag ctaccagagt 6001 aaatgagcaa atgaataaat gagtagatga attttagcgg ctaaaggagg cggcatggaa 6061 aatcaagaac aaccaggcac cgacgccgtg gaatgcccca tgtgtggagg aacgggcggt 6121 tggccaggcg taagcggctg ggttgtctgc cggccctgca atggcactgg aacccccaag 6181 cccgaggaat cggcgtgagc ggtcgcaaac catccggccc ggtacaaatc ggcgcggcgc 6241 tgggtgatga cctggtggag aagttgaagg cggcgcaggc cgcccagcgg caacgcatcg 6301 aggcagaagc acgccccggt gaatcgtggc aagcggccgc tgatcgaatc cgcaaagaat 6361 cccggcaacc gccggcagcc ggtgcgccgt cgattaggaa gccgcccaag ggcgacgagc 6421 aaccagattt tttcgttccg atgctctatg acgtgggcac ccgcgatagt cgcagcatca 6481 tggacgtggc cgttttccgt ctgtcgaagc gtgaccgacg agctggcgag gtgatccgct 6541 acgagcttcc agacgggcac gtagaggttt ccgcagggcc ggccggcatg gcgagtgtgt 6601 gggattacga cctggtactg atggcggttt cccatctaac cgaatccatg aaccgatacc 6661 gggaagggaa gggagacaag cccggccgcg tgttccgtcc acacgttgcg gacgtactca 6721 agttctgccg gcgagccgat ggcggaaagc agaaagacga cctggtagaa acctgcattc 6781 ggttaaacac cacgcacgtt gccatgcagc gtacgaagaa ggccaagaac ggccgcctgg 6841 tgacggtatc cgagggtgaa gccttgatta gccgctacaa gatcgtaaag agcgaaaccg 6901 ggcggccgga gtacatcgag atcgagctag ctgattggat gtaccgcgag atcacagaag 6961 gcaagaaccc ggacgtgctg acggttcacc ccgattactt tttgatcgat cccggcatcg 7021 gccgttttct ctaccgcctg gcacgccgcg ccgcaggcaa ggcagaagcc agatggttgt 7081 tcaagacgat ctacgaacgc agtggcagcg ccggagagtt caagaagttc tgtttcaccg 7141 tgcgcaagct gatcgggtca aatgacctgc cggagtacga tttgaaggag gaggcggggc 7201 aggctggccc gatcctagtc atgcgctacc gcaacctgat cgagggcgaa gcatccgccg 7261 gttcctaatg tacggagcag atgctagggc aaattgccct agcaggggaa aaaggtcgaa 7321 aaggtctctt tcctgtggat agcacgtaca ttgggaaccc aaagccgtac attgggaacc 7381 ggaacccgta cattgggaac ccaaagccgt acattgggaa ccggtcacac atgtaagtga 7441 ctgatataaa agagaaaaaa ggcgattttt ccgcctaaaa ctctttaaaa cttattaaaa 7501 ctcttaaaac ccgcctggcc tgtgcataac tgtctggcca gcgcacagcc gaagagctgc 7561 aaaaagcgcc tacccttcgg tcgctgcgct ccctacgccc cgccgcttcg cgtcggccta 7621 tcgcggccgc tggccgctca aaaatggctg gcctacggcc aggcaatcta ccagggcgcg 7681 gacaagccgc gccgtcgcca ctcgaccgcc ggcgcccaca tcaaggcacc ggtgggtatg 7741 cctgacgatg cgtggagacc gaaaccttgc gctcgttcgc cagccaggac agaaatgcct 7801 cgacttcgct gctgcccaag gttgccgggt gacgcacacc gtggaaacgg atgaaggcac 7861 gaacccagtg gacataagcc tgttcggttc gtaagctgta atgcaagtag cgtatgcgct 7921 cacgcaactg gtccagaacc ttgaccgaac gcagcggtgg taacggcgca gtggcggttt 7981 tcatggcttg ttatgactgt ttttttgggg tacagtctat gcctcgggca tccaagcagc 8041 aagcgcgtta cgccgtgggt cgatgtttga tgttatggag cagcaacgat gttacgcagc 8101 agggcagtcg ccctaaaaca aagttaaaca tcatgaggga agcggtgatc gccgaagtat 8161 cgactcaact atcagaggta gttggcgtca tcgagcgcca tctcgaaccg acgttgctgg 8221 ccgtacattt gtacggctcc gcagtggatg gcggcctgaa gccacacagt gatattgatt 8281 tgctggttac ggtgaccgta aggcttgatg aaacaacgcg gcgagctttg atcaacgacc 8341 ttttggaaac ttcggcttcc cctggagaga gcgagattct ccgcgctgta gaagtcacca 8401 ttgttgtgca cgacgacatc attccgtggc gttatccagc taagcgcgaa ctgcaatttg 8461 gagaatggca gcgcaatgac attcttgcag gtatcttcga gccagccacg atcgacattg 8521 atctggctat cttgctgaca aaagcaagag aacatagcgt tgccttggta ggtccagcgg 8581 cggaggaact ctttgatccg gttcctgaac aggatctatt tgaggcgcta aatgaaacct 8641 taacgctatg gaactcgccg cccgactggg ctggcgatga gcgaaatgta gtgcttacgt 8701 tgtcccgcat ttggtacagc gcagtaaccg gcaaaatcgc gccgaaggat gtcgctgccg 8761 actgggcaat ggagcgcctg ccggcccagt atcagcccgt catacttgaa gctagacagg 8821 cttatcttgg acaagaagaa gatcgcttgg cctcgcgcgc agatcagttg gaagaatttg 8881 tccactacgt gaaaggcgag atcaccaagg tagtcggcaa ataatgtcta acaattcgtt 8941 caagccgacg ccgcttcgcg gcgcggctta actcaagcgt tagatgcact aagcacataa 9001 ttgctcacag ccaaactatc aggtcaagtc tgcttttatt atttttaagc gtgcataata 9061 agccctacac aaattgggag atatatcatg aaaggctggc tttttcttgt tatcgcaata 9121 gttggcgaag taatcgcaac atagcttgct tggtcgttcc gcgtgaacgt cggctcgatt 9181 gtacctgcgt tcaaatactt tgcgatcgtg ttgcgcgcct gcccggtgcg tcggctgatc 9241 tcacggatcg actgcttctc tcgcaacgcc atccgacgga tgatgtttaa aagtcccatg 9301 tggatcactc cgttgccccg tcgctcaccg tgttgggggg aaggtgcaca tggctcagtt 9361 ctcaatggaa attatctgcc taaccggctc agttctgcgt agaaaccaac atgcaagctc 9421 caccgggtgc aaagcggcag cggc //