LOCUS       B482p5o2x35s-LH        10587 bp    DNA     circular SYN 22-JAN-2016
DEFINITION  Ligation of 35s-Xba-xho* into B472p5oM-LH*
ACCESSION   B482p5o2x35s-LH
KEYWORDS    .
SOURCE      Unknown.
  ORGANISM  Unknown
            Unclassified.
REFERENCE   1  (bases 1 to 10587)
  AUTHORS   Self
  JOURNAL   Unpublished.
COMMENT     SECID/File created by Clone Manager, Scientific & Educational Software
COMMENT     SECNOTES|Vector molecule:  B472p5oM-LH*
               Fragment ends: XhoI and XbaI
               Fragment size: 9792
            Insert molecule:  35s-Xba-xho*
               Fragment ends: XbaI and XhoI
               Fragment size: 795
            Primer for gene synthesis
            35sXba	CCCGTCtctagaCGGGCCAACATGGT
            35sXho	GGGGCCCTCGAGAGAGATAGA
            Sequencing primers	
            LHX1	CTTTATTTCGACGTGTCTAC
FEATURES             Location/Qualifiers
     misc_feature    1..24
                     /gene="LB"
                     /product="Left Border"
                     /SECDrawAs="Region"
                     /SECStyleId=1
     misc_feature    314..1098
                     /gene="P2x35s"
                     /SECDrawAs="Region"
                     /SECStyleId=1
     CDS             1116..1856
                     /gene="EPSPS"
                     /translation="MAQINNMAQGIQTLNPNSNFHKPQVPKSSSFLVFGSKKLKNSAN
                     SMLVLKKDSIFMQKFCSFRISASVATACMLHGASSRPATARKSSGLSGTVRIPGDKSI
                     SHRSFMFGGLASGETRITGLLEGEDVINTGKAMQAMGARIRKEGDTWIIDGVGNGGLL
                     APEAPLDFGNAATGCRLTMGLVGVYDFDSTFIGDASLTKRPMGRVLNPLREMGVQVKS
                     EDGDRLPVTLRGPKTPTPITYRVPMASAQ"
                     /SECDrawAs="Gene"
                     /SECStyleId=1
                     /SECName="epsps"
     misc_feature    1857..2045
                     /gene="int"
                     /product="Intron STLS1"
                     /SECDrawAs="Gene"
                     /SECStyleId=1
                     /SECName="int"
                     /SECDescr="Intron STLS1"
     CDS             2046..2888
                     /gene="epsps"
                     /codon_start=3
                     /SECDrawAs="Gene"
                     /SECStyleId=1
     misc_feature    2898..3188
                     /gene="TE9"
                     /product="Terminator Rbcs-E9"
                     /SECDrawAs="Region"
                     /SECStyleId=1
     misc_feature    3364..3387
                     /gene="RB"
                     /product="Right Border"
                     /SECDrawAs="Region"
                     /SECStyleId=1
     misc_feature    3922..4619
                     /gene="ColE1"
                     /product="ColE1 ORI"
                     /SECDrawAs="Region"
                     /SECStyleId=1
     misc_feature    5168..8873
                     /gene="pVS1"
                     /product="origin of replication for agrobacteria"
                     /SECDrawAs="Region"
                     /SECStyleId=1
     CDS             9057..10067
                     /gene="Sm/Sp"
                     /product="Strep/Spec resistence"
                     /SECDrawAs="Gene"
                     /SECStyleId=1
ORIGIN      
        1 ggcaggatat attcaattgt aaatggcttc atgtccggga aatctacatg gatcagcaat
       61 gagtatgatg gtcaatatgg agaaaaagaa agagtaatta ccaatttttt ttcaattcaa
      121 aaatgtagat gtccgcagcg ttattataaa atgaaagtac attttgataa aacgacaaat
      181 tacgatccgt cgtatttata ggcgaaagca ataaacaaat tattctaatt cggaaatctt
      241 tatttcgacg tgtctacatt cacgtccaaa tgggggctta gatgagaaac ttcacgatcg
      301 gctctagacg ggccaacatg gtggagcacg acactctcgt ctactccaag aatatcaaag
      361 atacagtctc agaagaccaa agggctattg agacttttca acaaagggta atatcgggaa
      421 acctcctcgg attccattgc ccagctatct gtcacttcat caaaaggaca gtagaaaagg
      481 aaggtggcac ctacaaatgc catcattgcg ataaaggaaa ggctatcgtt caagatgcct
      541 ctgccgacag tggtcccaaa gatggacccc cacccacgag gagcatcgtg gaaaaagaag
      601 acgttccaac cacgtcttca aagcaagtgg attgatgtga acatggtgga gcacgacact
      661 ctcgtctact ccaagaatat caaagataca gtctcagaag accaaagggc tattgagact
      721 tttcaacaaa gggtaatatc gggaaacctc ctcggattcc attgcccagc tatctgtcac
      781 ttcatcaaaa ggacagtaga aaaggaaggt ggcacctaca aatgccatca ttgcgataaa
      841 ggaaaggcta tcgttcaaga tgcctctgcc gacagtggtc ccaaagatgg acccccaccc
      901 acgaggagca tcgtggaaaa agaagacctt ccaaccacgt cttcaaagca agtggattga
      961 tgtgatatct ccactgacgt aagggatgac gcacaatccc actatccttc gcaagaccct
     1021 tcctctatat aaggaagttc atttcatttg gagaggacac gctgaaatca ccagtctctc
     1081 tctacaaatc tatctctctc gagtcctcca ccgaaatggc ccagatcaac aacatggctc
     1141 agggcattca gaccctcaac cccaattcca acttccacaa gccacaagtg cctaagtcta
     1201 gctccttcct cgtcttcggc tcgaagaagt tgaagaacag cgccaactcc atgcttgttc
     1261 tcaagaaaga ctcaatcttc atgcagaagt tttgcagctt ccgcatctcc gcaagtgtgg
     1321 ccactgcttg catgttgcac ggtgcctcca gccgtccggc gaccgccagg aagtcttccg
     1381 gcctctcggg gaccgtcaga atccccggag acaagagcat ttcccaccgc tcattcatgt
     1441 tcggcggcct ggccagcgga gagacaagga tcaccggcct tctggagggt gaggatgtca
     1501 tcaacaccgg gaaggctatg caggctatgg gagcccgcat ccggaaggaa ggcgacacgt
     1561 ggattatcga cggcgtggga aacggcggtc tcctcgcccc agaggcaccc ttggacttcg
     1621 gcaacgccgc caccggatgc cgtctgacta tggggcttgt tggcgtgtac gatttcgact
     1681 ccaccttcat cggagacgct tccctcacca agcgccctat gggcagggtc ctaaatccgc
     1741 tccgtgagat gggcgtgcaa gtcaagagcg aggacggaga ccgcctgcca gttaccttgc
     1801 gaggccccaa gactccaaca cccatcacct acagggtgcc tatggcctct gcgcaggtaa
     1861 gtttctgctt ctacctttga tatatatata ataattatca ttaattagta gtaatataat
     1921 atttcaaata tttttttcaa aataaaagaa tgtagtatat agcaattgct tttctgtagt
     1981 ttataagtgt gtatatttta atttataact tttctaatat atgaccaaaa tttgttgatg
     2041 tgcaggtcaa gtccgccgtg ctccttgccg ggctgaacac tccgggtatt accaccgtca
     2101 tcgagcccat tatgaccaga gatcataccg agaagatgct ccagggattc ggcgctaact
     2161 tgacggttga aaccgacgcc gacggcgtgc gcaccatccg tctggaggga aggggcaaac
     2221 tcaccggcca agtcatcgac gtgccaggag atcccagctc gactgctttt ccacttgtag
     2281 ccgccctctt ggtccctggt tccgacgtga ccatcctgaa cgttcttatg aacccgaccc
     2341 gcaccgggct catcttgact ctccaggaga tgggcgctga catcgaagtc attaacccca
     2401 ggctggccgg aggcgaggac gtggcagatt tgcgcgtccg tagttccacc ctgaagggtg
     2461 tgacagttcc agaggaccgg gctcccagca tgatcgacga gtaccctatc cttgccgtcg
     2521 ccgcggcctt cgccgagggc gctaccgtga tgaacggatt ggaggagctg cgcgtcaagg
     2581 agtcagacag actctccgcc gtggccaatg ggctcaagct caacggcgtt gactgcgatg
     2641 agggagaaac cagccttgtc gtgcgtggca ggccggacgg caagggactc ggtaacgctt
     2701 ctggggccgc agtcgccacg cacctggacc accgcatcgc gatgtccttc ctcgtgatgg
     2761 gccttgtttc ggagaaccca gtcaccgtgg acgatgccac catgattgct actagcttcc
     2821 ccgagttcat ggacttgatg gccggactgg gagccaagat cgagctgtcc gacaccaagg
     2881 ctgcctaacg cgttcgagta ttatggcatt gggaaaactg tttttcttgt accatttgtt
     2941 gtgcttgtaa tttactgtgt tttttattcg gttttcgcta tcgaactgtg aaatggaaat
     3001 ggatggagaa gagttaatga atgatatggt ccttttgttc attctcaaat taatattatt
     3061 tgttttttct cttatttgtt gtgtgttgaa tttgaaatta taagagatat gcaaacattt
     3121 tgttttgagt aaaaatgtgt caaatcgtgg cctctaatga ccgaagttaa tatgaggagt
     3181 aaaacactga agcctgcagg catgcaagct gatccactag aggccatggc ggccgcacta
     3241 gtggatcccc cgggatgcat gaattcaagc ttatcgatgt cgacaggcct taagggccag
     3301 atcttgggcc cggtacccga tcagattgtc gtttcccgcc ttcggtttaa actatcagtg
     3361 tttgacagga tatattggcg ggtaaaccta agagaaaaga gcgtttatta gaataatcgg
     3421 atatttaaaa gggcgtgaaa aggtttatcc gttcgtccat ttgtatgtgc atgccaacca
     3481 cagggttccc ctcgggagtg cttggcattc cgtgcgataa tgacttctgt tcaaccaccc
     3541 aaacgtcgga aagcctgacg acggagcagc attccaaaaa gatcccttgg ctcgtctggg
     3601 tcggctagaa ggtcgagtgg gctgctgtgg cttgatccct caacgcggtc gcggacgtag
     3661 cgcagcgccg aaaaatcctc gatcgcaaat ccgacgctgt cgaaaagcgt gatctgcttg
     3721 tcgctctttc ggccgacgtc ctggccagtc atcacgcgcc aaagttccgt cacaggatga
     3781 tctggcgcga gttgctggat ctcgccttca atccgggtct gtggcgggaa ctccacgaaa
     3841 atatccgaac gcagcaagat atcgcggtgc atctcggtct tgcctgggca gtcgccgccg
     3901 acgccgttga tgtggacgcc gaaaaggatc taggtgaaga tcctttttga taatctcatg
     3961 accaaaatcc cttaacgtga gttttcgttc cactgagcgt cagaccccgt agaaaagatc
     4021 aaaggatctt cttgagatcc tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa
     4081 ccaccgctac cagcggtggt ttgtttgccg gatcaagagc taccaactct ttttccgaag
     4141 gtaactggct tcagcagagc gcagatacca aatactgttc ttctagtgta gccgtagtta
     4201 ggccaccact tcaagaactc tgtagcaccg cctacatacc tcgctctgct aatcctgtta
     4261 ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg ggttggactc aagacgatag
     4321 ttaccggata aggcgcagcg gtcgggctga acggggggtt cgtgcacaca gcccagcttg
     4381 gagcgaacga cctacaccga actgagatac ctacagcgtg agctatgaga aagcgccacg
     4441 cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg aacaggagag
     4501 cgcacgaggg agcttccagg gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc
     4561 cacctctgac ttgagcgtcg atttttgtga tgctcgtcag gggggcggag cctatggaaa
     4621 aacgccagca acgcggcctt tttacggttc ctggcctttt gctggccttt tgctcacatg
     4681 ttctttcctg cgttatcccc tgattctgtg gataaccgat taccgccttt gagtgagctg
     4741 ataccgctcg ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaag
     4801 agcgcctgat gcggtatttt ctccttacgc atctgtgcgg tatttcacac cgcatatggt
     4861 gcactctcag tacaatctgc tctgatgccg catagttaag ccagtataca ctccgctatc
     4921 gctacgtgac tgggtcatgg ctgcgccccg acacccgcca acacccgctg acgcgccctg
     4981 acgggcttgt ctgctcccgg catccgctta cagacaagct gtgaccgtct ccgggagctg
     5041 catgtgtcag aggttttcac cgtcatcacc gaaacgcgcg aggcaggggt acgtcgaggt
     5101 cgatccaacc cctccgctgc tatagtgcag tcggcttctg acgttcagtg cagccgtctt
     5161 ctgaaaacga catgtcgcac aagtcctaag ttacgcgaca ggctgccgcc ctgccctttt
     5221 cctggcgttt tcttgtcgcg tgttttagtc gcataaagta gaatacttgc gactagaacc
     5281 ggagacatta cgccatgaac aagagcgccg ccgctggcct gctgggctat gcccgcgtca
     5341 gcaccgacga ccaggacttg accaaccaac gggccgaact gcacgcggcc ggctgcacca
     5401 agctgttttc cgagaagatc accggcacca ggcgcgaccg cccggagctg gccaggatgc
     5461 ttgaccacct acgccctggc gacgttgtga cagtgaccag gctagaccgc ctggcccgca
     5521 gcacccgcga cctactggac attgccgagc gcatccagga ggccggcgcg ggcctgcgta
     5581 gcctggcaga gccgtgggcc gacaccacca cgccggccgg ccgcatggtg ttgaccgtgt
     5641 tcgccggcat tgccgagttc gagcgttccc taatcatcga ccgcacccgg agcgggcgcg
     5701 aggccgccaa ggcgcgaggc gtgaagtttg gcccccgccc taccctcacc ccggcacaga
     5761 tcgcgcacgc ccgcgagctg atcgaccagg aaggccgcac cgtgaaagag gcggctgcac
     5821 tgcttggcgt gcatcgctcg accctgtacc gcgcacttga gcgcagcgag gaagtgacgc
     5881 ccaccgaggc caggcggcgc ggtgccttcc gtgaggacgc attgaccgag gccgacgccc
     5941 tggcggccgc cgagaatgaa cgccaagagg aacaagcatg aaaccgcacc aggacggcca
     6001 ggacgaaccg tttttcatta ccgaagagat cgaggcggag atgatcgcgg ccgggtacgt
     6061 gttcgagccg cccgcgcacg tctcaaccgt gcggctgcat gaaatcctgg ccggtttgtc
     6121 tgatgccaag ctcgcggcct ggccggcgag cttggccgct gaagaaaccg agcgccgccg
     6181 tctaaaaagg tgatgtgtat ttgagtaaaa cagcttgcgt catgcggtcg ctgcgtatat
     6241 gatgcgatga gtaaataaac aaatacgcaa ggggaacgca tgaaggttat cgctgtactt
     6301 aaccagaaag gcgggtcagg caagacgacc atcgcaaccc atctagcccg cgccctgcaa
     6361 ctcgccgggg ccgatgttct gttagtcgat tccgatcccc agggcagtgc ccgcgattgg
     6421 gcggccgtgc gggaagatca accgctaacc gttgtcggca tcgaccgccc gacgattgac
     6481 cgcgacgtga aggccatcgg ccggcgcgac ttcgtagtga tcgacggagc gccccaggcg
     6541 gcggacttgg ctgtgtccgc gatcaaggca gccgacttcg tgctgattcc ggtgcagcca
     6601 agcccttacg acatatgggc caccgccgac ctggtggagc tggttaagca gcgcattgag
     6661 gtcacggatg gaaggctaca agcggccttt gtcgtgtcgc gggcgatcaa aggcacgcgc
     6721 atcggcggtg aggttgccga ggcgctggcc gggtacgagc tgcccattct tgagtcccgt
     6781 atcacgcagc gcgtgagcta cccaggcact gccgccgccg gcacaaccgt tcttgaatca
     6841 gaacccgagg gcgacgctgc ccgcgaggtc caggcgctgg ccgctgaaat taaatcaaaa
     6901 ctcatttgag ttaatgaggt aaagagaaaa tgagcaaaag cacaaacacg ctaagtgccg
     6961 gccgtccgag cgcacgcagc agcaaggctg caacgttggc cagcctggca gacacgccag
     7021 ccatgaagcg ggtcaacttt cagttgccgg cggaggatca caccaagctg aagatgtacg
     7081 cggtacgcca aggcaagacc attaccgagc tgctatctga atacatcgcg cagctaccag
     7141 agtaaatgag caaatgaata aatgagtaga tgaattttag cggctaaagg aggcggcatg
     7201 gaaaatcaag aacaaccagg caccgacgcc gtggaatgcc ccatgtgtgg aggaacgggc
     7261 ggttggccag gcgtaagcgg ctgggttgtc tgccggccct gcaatggcac tggaaccccc
     7321 aagcccgagg aatcggcgtg agcggtcgca aaccatccgg cccggtacaa atcggcgcgg
     7381 cgctgggtga tgacctggtg gagaagttga aggcggcgca ggccgcccag cggcaacgca
     7441 tcgaggcaga agcacgcccc ggtgaatcgt ggcaagcggc cgctgatcga atccgcaaag
     7501 aatcccggca accgccggca gccggtgcgc cgtcgattag gaagccgccc aagggcgacg
     7561 agcaaccaga ttttttcgtt ccgatgctct atgacgtggg cacccgcgat agtcgcagca
     7621 tcatggacgt ggccgttttc cgtctgtcga agcgtgaccg acgagctggc gaggtgatcc
     7681 gctacgagct tccagacggg cacgtagagg tttccgcagg gccggccggc atggcgagtg
     7741 tgtgggatta cgacctggta ctgatggcgg tttcccatct aaccgaatcc atgaaccgat
     7801 accgggaagg gaagggagac aagcccggcc gcgtgttccg tccacacgtt gcggacgtac
     7861 tcaagttctg ccggcgagcc gatggcggaa agcagaaaga cgacctggta gaaacctgca
     7921 ttcggttaaa caccacgcac gttgccatgc agcgtacgaa gaaggccaag aacggccgcc
     7981 tggtgacggt atccgagggt gaagccttga ttagccgcta caagatcgta aagagcgaaa
     8041 ccgggcggcc ggagtacatc gagatcgagc tagctgattg gatgtaccgc gagatcacag
     8101 aaggcaagaa cccggacgtg ctgacggttc accccgatta ctttttgatc gatcccggca
     8161 tcggccgttt tctctaccgc ctggcacgcc gcgccgcagg caaggcagaa gccagatggt
     8221 tgttcaagac gatctacgaa cgcagtggca gcgccggaga gttcaagaag ttctgtttca
     8281 ccgtgcgcaa gctgatcggg tcaaatgacc tgccggagta cgatttgaag gaggaggcgg
     8341 ggcaggctgg cccgatccta gtcatgcgct accgcaacct gatcgagggc gaagcatccg
     8401 ccggttccta atgtacggag cagatgctag ggcaaattgc cctagcaggg gaaaaaggtc
     8461 gaaaaggtct ctttcctgtg gatagcacgt acattgggaa cccaaagccg tacattggga
     8521 accggaaccc gtacattggg aacccaaagc cgtacattgg gaaccggtca cacatgtaag
     8581 tgactgatat aaaagagaaa aaaggcgatt tttccgccta aaactcttta aaacttatta
     8641 aaactcttaa aacccgcctg gcctgtgcat aactgtctgg ccagcgcaca gccgaagagc
     8701 tgcaaaaagc gcctaccctt cggtcgctgc gctccctacg ccccgccgct tcgcgtcggc
     8761 ctatcgcggc cgctggccgc tcaaaaatgg ctggcctacg gccaggcaat ctaccagggc
     8821 gcggacaagc cgcgccgtcg ccactcgacc gccggcgccc acatcaaggc accggtgggt
     8881 atgcctgacg atgcgtggag accgaaacct tgcgctcgtt cgccagccag gacagaaatg
     8941 cctcgacttc gctgctgccc aaggttgccg ggtgacgcac accgtggaaa cggatgaagg
     9001 cacgaaccca gtggacataa gcctgttcgg ttcgtaagct gtaatgcaag tagcgtatgc
     9061 gctcacgcaa ctggtccaga accttgaccg aacgcagcgg tggtaacggc gcagtggcgg
     9121 ttttcatggc ttgttatgac tgtttttttg gggtacagtc tatgcctcgg gcatccaagc
     9181 agcaagcgcg ttacgccgtg ggtcgatgtt tgatgttatg gagcagcaac gatgttacgc
     9241 agcagggcag tcgccctaaa acaaagttaa acatcatgag ggaagcggtg atcgccgaag
     9301 tatcgactca actatcagag gtagttggcg tcatcgagcg ccatctcgaa ccgacgttgc
     9361 tggccgtaca tttgtacggc tccgcagtgg atggcggcct gaagccacac agtgatattg
     9421 atttgctggt tacggtgacc gtaaggcttg atgaaacaac gcggcgagct ttgatcaacg
     9481 accttttgga aacttcggct tcccctggag agagcgagat tctccgcgct gtagaagtca
     9541 ccattgttgt gcacgacgac atcattccgt ggcgttatcc agctaagcgc gaactgcaat
     9601 ttggagaatg gcagcgcaat gacattcttg caggtatctt cgagccagcc acgatcgaca
     9661 ttgatctggc tatcttgctg acaaaagcaa gagaacatag cgttgccttg gtaggtccag
     9721 cggcggagga actctttgat ccggttcctg aacaggatct atttgaggcg ctaaatgaaa
     9781 ccttaacgct atggaactcg ccgcccgact gggctggcga tgagcgaaat gtagtgctta
     9841 cgttgtcccg catttggtac agcgcagtaa ccggcaaaat cgcgccgaag gatgtcgctg
     9901 ccgactgggc aatggagcgc ctgccggccc agtatcagcc cgtcatactt gaagctagac
     9961 aggcttatct tggacaagaa gaagatcgct tggcctcgcg cgcagatcag ttggaagaat
    10021 ttgtccacta cgtgaaaggc gagatcacca aggtagtcgg caaataatgt ctaacaattc
    10081 gttcaagccg acgccgcttc gcggcgcggc ttaactcaag cgttagatgc actaagcaca
    10141 taattgctca cagccaaact atcaggtcaa gtctgctttt attattttta agcgtgcata
    10201 ataagcccta cacaaattgg gagatatatc atgaaaggct ggctttttct tgttatcgca
    10261 atagttggcg aagtaatcgc aacatagctt gcttggtcgt tccgcgtgaa cgtcggctcg
    10321 attgtacctg cgttcaaata ctttgcgatc gtgttgcgcg cctgcccggt gcgtcggctg
    10381 atctcacgga tcgactgctt ctctcgcaac gccatccgac ggatgatgtt taaaagtccc
    10441 atgtggatca ctccgttgcc ccgtcgctca ccgtgttggg gggaaggtgc acatggctca
    10501 gttctcaatg gaaattatct gcctaaccgg ctcagttctg cgtagaaacc aacatgcaag
    10561 ctccaccggg tgcaaagcgg cagcggc
//
