LOCUS B485p9o2x35s-LH 9815 bp DNA circular SYN 22-JAN-2016 DEFINITION Ligation of 35s-Xba-xho* into B475p9oM-LH* ACCESSION B485p9o2x35s-LH KEYWORDS . SOURCE Unknown. ORGANISM Unknown Unclassified. REFERENCE 1 (bases 1 to 9815) AUTHORS Self JOURNAL Unpublished. COMMENT SECID/File created by Clone Manager, Scientific & Educational Software COMMENT SECNOTES|Vector molecule: B475p9oM-LH* Fragment ends: XhoI and XbaI Fragment size: 9020 Insert molecule: 35s-Xba-xho* Fragment ends: XbaI and XhoI Fragment size: 795 Primer for gene synthesis 35sXba CCCGTCtctagaCGGGCCAACATGGT 35sXho GGGGCCCTCGAGAGAGATAGA Sequencing primers LHX1 CTTTATTTCGACGTGTCTAC FEATURES Location/Qualifiers misc_feature 1..24 /gene="LB" /product="Left Border" /SECDrawAs="Region" /SECStyleId=1 misc_feature 314..1098 /gene="P2x35s" /SECDrawAs="Region" /SECStyleId=1 CDS 1116..1414 /gene="nptIIo" /product="NptII optimiert" /SECDrawAs="Gene" /SECStyleId=1 CDS 1415..1601 /gene="int" /SECDrawAs="Gene" /SECStyleId=1 CDS 1602..2108 /gene="nptIIo" /product="NptII optimiert" /SECDrawAs="Gene" /SECStyleId=1 misc_feature 2126..2416 /gene="TE9" /product="Terminator Rbcs-E9" /SECDrawAs="Region" /SECStyleId=1 misc_feature 2592..2615 /gene="RB" /product="Right Border" /SECDrawAs="Region" /SECStyleId=1 misc_feature 3150..3847 /gene="ColE1" /product="ColE1 ORI" /SECDrawAs="Region" /SECStyleId=1 misc_feature 4396..8101 /gene="pVS1" /product="origin of replication for agrobacteria" /SECDrawAs="Region" /SECStyleId=1 CDS 8285..9295 /gene="Sm/Sp" /product="Strep/Spec resistence" /SECDrawAs="Gene" /SECStyleId=1 ORIGIN 1 ggcaggatat attcaattgt aaatggcttc atgtccggga aatctacatg gatcagcaat 61 gagtatgatg gtcaatatgg agaaaaagaa agagtaatta ccaatttttt ttcaattcaa 121 aaatgtagat gtccgcagcg ttattataaa atgaaagtac attttgataa aacgacaaat 181 tacgatccgt cgtatttata ggcgaaagca ataaacaaat tattctaatt cggaaatctt 241 tatttcgacg tgtctacatt cacgtccaaa tgggggctta gatgagaaac ttcacgatcg 301 gctctagacg ggccaacatg gtggagcacg acactctcgt ctactccaag aatatcaaag 361 atacagtctc agaagaccaa agggctattg agacttttca acaaagggta atatcgggaa 421 acctcctcgg attccattgc ccagctatct gtcacttcat caaaaggaca gtagaaaagg 481 aaggtggcac ctacaaatgc catcattgcg ataaaggaaa ggctatcgtt caagatgcct 541 ctgccgacag tggtcccaaa gatggacccc cacccacgag gagcatcgtg gaaaaagaag 601 acgttccaac cacgtcttca aagcaagtgg attgatgtga acatggtgga gcacgacact 661 ctcgtctact ccaagaatat caaagataca gtctcagaag accaaagggc tattgagact 721 tttcaacaaa gggtaatatc gggaaacctc ctcggattcc attgcccagc tatctgtcac 781 ttcatcaaaa ggacagtaga aaaggaaggt ggcacctaca aatgccatca ttgcgataaa 841 ggaaaggcta tcgttcaaga tgcctctgcc gacagtggtc ccaaagatgg acccccaccc 901 acgaggagca tcgtggaaaa agaagacctt ccaaccacgt cttcaaagca agtggattga 961 tgtgatatct ccactgacgt aagggatgac gcacaatccc actatccttc gcaagaccct 1021 tcctctatat aaggaagttc atttcatttg gagaggacac gctgaaatca ccagtctctc 1081 tctacaaatc tatctctctc gagtcctcca ccgaaatggg agcatggatt gagcaggatg 1141 gcctccatgc tggtagcccc gctgcttggg tggagcgcct ctttggctac gactgggccc 1201 aacagaccat tgggtgcagt gatgccgctg tcttccgttt gagcgcccag ggcaggccag 1261 ttctcttcgt gaagactgac ctctccggtg ccctcaacga gttgcaggac gaagctgccc 1321 gcctctcatg gcttgccacc accggagtcc cttgcgctgc cgtgttggac gttgtcacag 1381 aggccggaag agactggctg ctcttgggcg aggtaagttt ctgcttctac ctttgatata 1441 tatataataa ttatcattaa ttagtagtaa tataatattt caaatatttt tttcaaaata 1501 aaagaatgta gtatatagca attgcttttc tgtagtttat aagtgtgtat attttaattt 1561 ataacttttc taatatatga ccaaaatttg ttgatgtgca ggtgccaggc caagacctcc 1621 tgagctccca cctggcccca gccgagaagg tctccatcat ggcagacgcc atgcgtcgcc 1681 tccacaccct ggacccagcc acctgcccct tcgaccacca ggccaagcac aggatcgaac 1741 gtgcacgcac taggatggag gccggccttg tggaccagga tgaccttgac gaggagcacc 1801 agggccttgc tcccgccgag ctgttcgccc gcctcaaggc tagaatgcct gacggagagg 1861 acttggtggt cacccacggg gatgcctgcc tccccaacat catggttgag aacggacgct 1921 tctctggctt catcgactgc ggtcgtctgg gcgtggccga ccgctaccaa gacatcgccc 1981 tggccaccag ggacatcgct gaggaacttg gaggggagtg ggccgatcgt ttcttggtcc 2041 tctacggcat tgctgccccc gactcccaga ggatcgcctt ctaccgcctg cttgacgagt 2101 tcttctgatc cggaatcgcg ttcgagtatt atggcattgg gaaaactgtt tttcttgtac 2161 catttgttgt gcttgtaatt tactgtgttt tttattcggt tttcgctatc gaactgtgaa 2221 atggaaatgg atggagaaga gttaatgaat gatatggtcc ttttgttcat tctcaaatta 2281 atattatttg ttttttctct tatttgttgt gtgttgaatt tgaaattata agagatatgc 2341 aaacattttg ttttgagtaa aaatgtgtca aatcgtggcc tctaatgacc gaagttaata 2401 tgaggagtaa aacactgaag cctgcaggca tgcaagctga tccactagag gccatggcgg 2461 ccgcactagt ggatcccccg ggatgcatga attcaagctt atcgatgtcg acaggcctta 2521 agggccagat cttgggcccg gtacccgatc agattgtcgt ttcccgcctt cggtttaaac 2581 tatcagtgtt tgacaggata tattggcggg taaacctaag agaaaagagc gtttattaga 2641 ataatcggat atttaaaagg gcgtgaaaag gtttatccgt tcgtccattt gtatgtgcat 2701 gccaaccaca gggttcccct cgggagtgct tggcattccg tgcgataatg acttctgttc 2761 aaccacccaa acgtcggaaa gcctgacgac ggagcagcat tccaaaaaga tcccttggct 2821 cgtctgggtc ggctagaagg tcgagtgggc tgctgtggct tgatccctca acgcggtcgc 2881 ggacgtagcg cagcgccgaa aaatcctcga tcgcaaatcc gacgctgtcg aaaagcgtga 2941 tctgcttgtc gctctttcgg ccgacgtcct ggccagtcat cacgcgccaa agttccgtca 3001 caggatgatc tggcgcgagt tgctggatct cgccttcaat ccgggtctgt ggcgggaact 3061 ccacgaaaat atccgaacgc agcaagatat cgcggtgcat ctcggtcttg cctgggcagt 3121 cgccgccgac gccgttgatg tggacgccga aaaggatcta ggtgaagatc ctttttgata 3181 atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag 3241 aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa 3301 caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt 3361 ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgttctt ctagtgtagc 3421 cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa 3481 tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa 3541 gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc 3601 ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa 3661 gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa 3721 caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg 3781 ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc 3841 tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg 3901 ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgatta ccgcctttga 3961 gtgagctgat accgctcgcc gcagccgaac gaccgagcgc agcgagtcag tgagcgagga 4021 agcggaagag cgcctgatgc ggtattttct ccttacgcat ctgtgcggta tttcacaccg 4081 catatggtgc actctcagta caatctgctc tgatgccgca tagttaagcc agtatacact 4141 ccgctatcgc tacgtgactg ggtcatggct gcgccccgac acccgccaac acccgctgac 4201 gcgccctgac gggcttgtct gctcccggca tccgcttaca gacaagctgt gaccgtctcc 4261 gggagctgca tgtgtcagag gttttcaccg tcatcaccga aacgcgcgag gcaggggtac 4321 gtcgaggtcg atccaacccc tccgctgcta tagtgcagtc ggcttctgac gttcagtgca 4381 gccgtcttct gaaaacgaca tgtcgcacaa gtcctaagtt acgcgacagg ctgccgccct 4441 gcccttttcc tggcgttttc ttgtcgcgtg ttttagtcgc ataaagtaga atacttgcga 4501 ctagaaccgg agacattacg ccatgaacaa gagcgccgcc gctggcctgc tgggctatgc 4561 ccgcgtcagc accgacgacc aggacttgac caaccaacgg gccgaactgc acgcggccgg 4621 ctgcaccaag ctgttttccg agaagatcac cggcaccagg cgcgaccgcc cggagctggc 4681 caggatgctt gaccacctac gccctggcga cgttgtgaca gtgaccaggc tagaccgcct 4741 ggcccgcagc acccgcgacc tactggacat tgccgagcgc atccaggagg ccggcgcggg 4801 cctgcgtagc ctggcagagc cgtgggccga caccaccacg ccggccggcc gcatggtgtt 4861 gaccgtgttc gccggcattg ccgagttcga gcgttcccta atcatcgacc gcacccggag 4921 cgggcgcgag gccgccaagg cgcgaggcgt gaagtttggc ccccgcccta ccctcacccc 4981 ggcacagatc gcgcacgccc gcgagctgat cgaccaggaa ggccgcaccg tgaaagaggc 5041 ggctgcactg cttggcgtgc atcgctcgac cctgtaccgc gcacttgagc gcagcgagga 5101 agtgacgccc accgaggcca ggcggcgcgg tgccttccgt gaggacgcat tgaccgaggc 5161 cgacgccctg gcggccgccg agaatgaacg ccaagaggaa caagcatgaa accgcaccag 5221 gacggccagg acgaaccgtt tttcattacc gaagagatcg aggcggagat gatcgcggcc 5281 gggtacgtgt tcgagccgcc cgcgcacgtc tcaaccgtgc ggctgcatga aatcctggcc 5341 ggtttgtctg atgccaagct cgcggcctgg ccggcgagct tggccgctga agaaaccgag 5401 cgccgccgtc taaaaaggtg atgtgtattt gagtaaaaca gcttgcgtca tgcggtcgct 5461 gcgtatatga tgcgatgagt aaataaacaa atacgcaagg ggaacgcatg aaggttatcg 5521 ctgtacttaa ccagaaaggc gggtcaggca agacgaccat cgcaacccat ctagcccgcg 5581 ccctgcaact cgccggggcc gatgttctgt tagtcgattc cgatccccag ggcagtgccc 5641 gcgattgggc ggccgtgcgg gaagatcaac cgctaaccgt tgtcggcatc gaccgcccga 5701 cgattgaccg cgacgtgaag gccatcggcc ggcgcgactt cgtagtgatc gacggagcgc 5761 cccaggcggc ggacttggct gtgtccgcga tcaaggcagc cgacttcgtg ctgattccgg 5821 tgcagccaag cccttacgac atatgggcca ccgccgacct ggtggagctg gttaagcagc 5881 gcattgaggt cacggatgga aggctacaag cggcctttgt cgtgtcgcgg gcgatcaaag 5941 gcacgcgcat cggcggtgag gttgccgagg cgctggccgg gtacgagctg cccattcttg 6001 agtcccgtat cacgcagcgc gtgagctacc caggcactgc cgccgccggc acaaccgttc 6061 ttgaatcaga acccgagggc gacgctgccc gcgaggtcca ggcgctggcc gctgaaatta 6121 aatcaaaact catttgagtt aatgaggtaa agagaaaatg agcaaaagca caaacacgct 6181 aagtgccggc cgtccgagcg cacgcagcag caaggctgca acgttggcca gcctggcaga 6241 cacgccagcc atgaagcggg tcaactttca gttgccggcg gaggatcaca ccaagctgaa 6301 gatgtacgcg gtacgccaag gcaagaccat taccgagctg ctatctgaat acatcgcgca 6361 gctaccagag taaatgagca aatgaataaa tgagtagatg aattttagcg gctaaaggag 6421 gcggcatgga aaatcaagaa caaccaggca ccgacgccgt ggaatgcccc atgtgtggag 6481 gaacgggcgg ttggccaggc gtaagcggct gggttgtctg ccggccctgc aatggcactg 6541 gaacccccaa gcccgaggaa tcggcgtgag cggtcgcaaa ccatccggcc cggtacaaat 6601 cggcgcggcg ctgggtgatg acctggtgga gaagttgaag gcggcgcagg ccgcccagcg 6661 gcaacgcatc gaggcagaag cacgccccgg tgaatcgtgg caagcggccg ctgatcgaat 6721 ccgcaaagaa tcccggcaac cgccggcagc cggtgcgccg tcgattagga agccgcccaa 6781 gggcgacgag caaccagatt ttttcgttcc gatgctctat gacgtgggca cccgcgatag 6841 tcgcagcatc atggacgtgg ccgttttccg tctgtcgaag cgtgaccgac gagctggcga 6901 ggtgatccgc tacgagcttc cagacgggca cgtagaggtt tccgcagggc cggccggcat 6961 ggcgagtgtg tgggattacg acctggtact gatggcggtt tcccatctaa ccgaatccat 7021 gaaccgatac cgggaaggga agggagacaa gcccggccgc gtgttccgtc cacacgttgc 7081 ggacgtactc aagttctgcc ggcgagccga tggcggaaag cagaaagacg acctggtaga 7141 aacctgcatt cggttaaaca ccacgcacgt tgccatgcag cgtacgaaga aggccaagaa 7201 cggccgcctg gtgacggtat ccgagggtga agccttgatt agccgctaca agatcgtaaa 7261 gagcgaaacc gggcggccgg agtacatcga gatcgagcta gctgattgga tgtaccgcga 7321 gatcacagaa ggcaagaacc cggacgtgct gacggttcac cccgattact ttttgatcga 7381 tcccggcatc ggccgttttc tctaccgcct ggcacgccgc gccgcaggca aggcagaagc 7441 cagatggttg ttcaagacga tctacgaacg cagtggcagc gccggagagt tcaagaagtt 7501 ctgtttcacc gtgcgcaagc tgatcgggtc aaatgacctg ccggagtacg atttgaagga 7561 ggaggcgggg caggctggcc cgatcctagt catgcgctac cgcaacctga tcgagggcga 7621 agcatccgcc ggttcctaat gtacggagca gatgctaggg caaattgccc tagcagggga 7681 aaaaggtcga aaaggtctct ttcctgtgga tagcacgtac attgggaacc caaagccgta 7741 cattgggaac cggaacccgt acattgggaa cccaaagccg tacattggga accggtcaca 7801 catgtaagtg actgatataa aagagaaaaa aggcgatttt tccgcctaaa actctttaaa 7861 acttattaaa actcttaaaa cccgcctggc ctgtgcataa ctgtctggcc agcgcacagc 7921 cgaagagctg caaaaagcgc ctacccttcg gtcgctgcgc tccctacgcc ccgccgcttc 7981 gcgtcggcct atcgcggccg ctggccgctc aaaaatggct ggcctacggc caggcaatct 8041 accagggcgc ggacaagccg cgccgtcgcc actcgaccgc cggcgcccac atcaaggcac 8101 cggtgggtat gcctgacgat gcgtggagac cgaaaccttg cgctcgttcg ccagccagga 8161 cagaaatgcc tcgacttcgc tgctgcccaa ggttgccggg tgacgcacac cgtggaaacg 8221 gatgaaggca cgaacccagt ggacataagc ctgttcggtt cgtaagctgt aatgcaagta 8281 gcgtatgcgc tcacgcaact ggtccagaac cttgaccgaa cgcagcggtg gtaacggcgc 8341 agtggcggtt ttcatggctt gttatgactg tttttttggg gtacagtcta tgcctcgggc 8401 atccaagcag caagcgcgtt acgccgtggg tcgatgtttg atgttatgga gcagcaacga 8461 tgttacgcag cagggcagtc gccctaaaac aaagttaaac atcatgaggg aagcggtgat 8521 cgccgaagta tcgactcaac tatcagaggt agttggcgtc atcgagcgcc atctcgaacc 8581 gacgttgctg gccgtacatt tgtacggctc cgcagtggat ggcggcctga agccacacag 8641 tgatattgat ttgctggtta cggtgaccgt aaggcttgat gaaacaacgc ggcgagcttt 8701 gatcaacgac cttttggaaa cttcggcttc ccctggagag agcgagattc tccgcgctgt 8761 agaagtcacc attgttgtgc acgacgacat cattccgtgg cgttatccag ctaagcgcga 8821 actgcaattt ggagaatggc agcgcaatga cattcttgca ggtatcttcg agccagccac 8881 gatcgacatt gatctggcta tcttgctgac aaaagcaaga gaacatagcg ttgccttggt 8941 aggtccagcg gcggaggaac tctttgatcc ggttcctgaa caggatctat ttgaggcgct 9001 aaatgaaacc ttaacgctat ggaactcgcc gcccgactgg gctggcgatg agcgaaatgt 9061 agtgcttacg ttgtcccgca tttggtacag cgcagtaacc ggcaaaatcg cgccgaagga 9121 tgtcgctgcc gactgggcaa tggagcgcct gccggcccag tatcagcccg tcatacttga 9181 agctagacag gcttatcttg gacaagaaga agatcgcttg gcctcgcgcg cagatcagtt 9241 ggaagaattt gtccactacg tgaaaggcga gatcaccaag gtagtcggca aataatgtct 9301 aacaattcgt tcaagccgac gccgcttcgc ggcgcggctt aactcaagcg ttagatgcac 9361 taagcacata attgctcaca gccaaactat caggtcaagt ctgcttttat tatttttaag 9421 cgtgcataat aagccctaca caaattggga gatatatcat gaaaggctgg ctttttcttg 9481 ttatcgcaat agttggcgaa gtaatcgcaa catagcttgc ttggtcgttc cgcgtgaacg 9541 tcggctcgat tgtacctgcg ttcaaatact ttgcgatcgt gttgcgcgcc tgcccggtgc 9601 gtcggctgat ctcacggatc gactgcttct ctcgcaacgc catccgacgg atgatgttta 9661 aaagtcccat gtggatcact ccgttgcccc gtcgctcacc gtgttggggg gaaggtgcac 9721 atggctcagt tctcaatgga aattatctgc ctaaccggct cagttctgcg tagaaaccaa 9781 catgcaagct ccaccgggtg caaagcggca gcggc //