LOCUS B488p6oAct-LH 10659 bp DNA circular SYN 22-JAN-2016 DEFINITION Ligation of A373p6ido-Act* into B473p6oM-LH* ACCESSION B488p6oAct-LH KEYWORDS . SOURCE Unknown. ORGANISM Unknown Unclassified. REFERENCE 1 (bases 1 to 10659) AUTHORS Self JOURNAL Unpublished. COMMENT SECID/File created by Clone Manager, Scientific & Educational Software COMMENT SECNOTES|Vector molecule: B473p6oM-LH* Fragment ends: XhoI and XbaI Fragment size: 9242 Insert molecule: A373p6ido-Act* Fragment ends: XbaI and XhoI Fragment size: 1417 GelR Sequencing primers Act1 CACTATCACCGTGAGTTGTC FEATURES Location/Qualifiers misc_feature 1..24 /gene="LB" /product="Left Border" /SECDrawAs="Region" /SECStyleId=1 misc_feature 313..1710 /gene="ACT" /product="Actin Promotor" /SECDrawAs="Region" /SECStyleId=1 CDS 1738..2307 /gene="hptII" /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGR GYVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQD LPETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVY HWQTVMDDTVSASVAQALDELMLWAEDCPE" /SECDrawAs="Gene" /SECStyleId=1 /SECName="hptII" misc_feature 2308..2496 /gene="int" /product="Intron StLS1" /SECDrawAs="Gene" /SECStyleId=1 /SECName="int" /SECDescr="Intron StLS1" CDS 2497..2952 /gene="hptII" /codon_start=3 /SECDrawAs="Gene" /SECStyleId=1 misc_feature 2970..3260 /gene="TE9" /product="Terminator Rbcs-E9" /SECDrawAs="Region" /SECStyleId=1 misc_feature 3436..3459 /gene="RB" /product="Right Border" /SECDrawAs="Region" /SECStyleId=1 misc_feature 3994..4691 /gene="ColE1" /product="ColE1 ORI" /SECDrawAs="Region" /SECStyleId=1 misc_feature 5240..8945 /gene="pVS1" /product="origin of replication for agrobacteria" /SECDrawAs="Region" /SECStyleId=1 CDS 9129..10139 /gene="Sm/Sp" /product="Strep/Spec resistence" /SECDrawAs="Gene" /SECStyleId=1 ORIGIN 1 ggcaggatat attcaattgt aaatggcttc atgtccggga aatctacatg gatcagcaat 61 gagtatgatg gtcaatatgg agaaaaagaa agagtaatta ccaatttttt ttcaattcaa 121 aaatgtagat gtccgcagcg ttattataaa atgaaagtac attttgataa aacgacaaat 181 tacgatccgt cgtatttata ggcgaaagca ataaacaaat tattctaatt cggaaatctt 241 tatttcgacg tgtctacatt cacgtccaaa tgggggctta gatgagaaac ttcacgatcg 301 gctctagaca ggtcgaggtc attcatatgc ttgagaagag agtcgggata gtccaaaata 361 aaacaaaggt aagattacct ggtcaaaagt gaaaacatca gttaaaaggt ggtataaagt 421 aaaatatcgg taataaaagg tggcccaaag tgaaatttac tcttttctac tattataaaa 481 attgaggatg tttttgtcgg tactttgata cgtcattttt gtatgaattg gtttttaagt 541 ttattcgctt ttggaaatgc atatctgtat ttgagtcggg ttttaagttc gtttgctttt 601 gtaaatacag agggatttgt ataagaaata tctttaaaaa aacccatatg ctaatttgac 661 ataatttttg agaaaaatat atattcaggc gaattctcac aatgaacaat aataagatta 721 aaatagcttt cccccgttgc agcgcatggg tattttttct agtaaaaata aaagataaac 781 ttagactcaa aacatttaca aaaacaaccc ctaaagttcc taaagcccaa agtgctatcc 841 acgatccata gcaagcccag cccaacccaa cccaacccaa cccaccccag tccagccaac 901 tggacaatag tctccacacc cccccactat caccgtgagt tgtccgcacg caccgcacgt 961 ctcgcagcca aaaaaaaaaa aagaaagaaa aaaaagaaaa agaaaaaaca gcaggtgggt 1021 ccgggtcgtg ggggccggaa acgcgaggag gatcgcgagc cagcgacgag gccggccctc 1081 cctccgcttc caaagaaacg ccccccatcg ccactatata catacccccc cctctcctcc 1141 catcccccca accctaccac caccaccacc accacctcca cctcctcccc cctcgctgcc 1201 ggacgacgag ctcctccccc ctccccctcc gccgccgccg cgccggtaac caccccgccc 1261 ctctcctctt tctttctccg tttttttttt ccgtctcggt ctcgatcttt ggccttggta 1321 gtttgggtgg gcgagaggcg gcttcgtgcg cgcccagatc ggtgcgcggg aggggcggga 1381 tctcgcggct ggggctctcg ccggcgtgga tccggcccgg atctcgcggg gaatggggct 1441 ctcggatgta gatctgcgat ccgccgttgt tgggggagat gatggggggt ttaaaatttc 1501 cgccatgcta aacaagatca ggaagagggg aaaagggcac tatggtttat atttttatat 1561 atttctgctg cttcgtcagg cttagatgtg ctagatcttt ctttcttctt tttgtgggta 1621 gaatttgaat ccctcagcat tgttcatcgg tagtttttct tttcatgatt tgtgacaaat 1681 gcagcctcgt gcggagcttt tttgtaggta ctaggctagc tcgagtcctc caccgaaatg 1741 aagaaaccgg agctgaccgc cacttccgtc gaaaagttcc tcatcgagaa gtttgatagc 1801 gtgtctgacc ttatgcagtt gtcggagggc gaagagtccc gcgcgttcag ctttgatgtt 1861 ggcgggcgcg ggtacgtcct ccgcgtgaac tcttgcgctg acggcttcta taaagatcgg 1921 tacgtttaca ggcatttcgc ctcggcggcc ctgcctattc ccgaagtcct cgacatcggc 1981 gagttttccg agagccttac gtattgtatt tcccgccggg ctcaaggggt gaccctccag 2041 gatctgccgg aaactgagtt gcctgcggtt ctccagcccg tcgccgaggc tatggacgcc 2101 atcgccgcgg cggatctttc tcaaacgtcg ggcttcgggc cgttcggccc tcaggggatt 2161 ggccagtaca ccacttggag ggactttatc tgcgctatcg ccgatccgca cgtgtatcat 2221 tggcaaaccg ttatggacga tacggtctcc gcgagcgtgg cccaggctct ggacgaactc 2281 atgttgtggg ccgaggattg ccccgaggta agtttctgct tctacctttg atatatatat 2341 aataattatc attaattagt agtaatataa tatttcaaat atttttttca aaataaaaga 2401 atgtagtata tagcaattgc ttttctgtag tttataagtg tgtatatttt aatttataac 2461 ttttctaata tatgaccaaa atttgttgat gtgcaggtcc gccacctggt tcatgcggac 2521 ttcggctcta ataacgtgct caccgataat gggcggatta ctgccgtcat cgactggtcc 2581 gaagctatgt ttggcgattc gcagtacgaa gtggcgaaca ttttcttctg gaggccttgg 2641 cttgcctgca tggaacagca aacgcgctac tttgagcgga ggcacccgga gctggcgggg 2701 tctccccgcc tcagggccta tatgttgcgg atcggcctcg accagcttta ccagtccctg 2761 gttgatggga atttcgacga tgctgcctgg gcgcaaggcc gctgtgacgc tattgtcagg 2821 agcggcgcgg ggaccgtggg ccgcactcag atcgcccggc ggtcggctgc cgtttggacg 2881 gatgggtgcg tcgaagtgct cgcggacagc ggcaacaggc ggccttccac ccgcccgcgg 2941 gccaaggagt gatccggaat cgcgttcgag tattatggca ttgggaaaac tgtttttctt 3001 gtaccatttg ttgtgcttgt aatttactgt gttttttatt cggttttcgc tatcgaactg 3061 tgaaatggaa atggatggag aagagttaat gaatgatatg gtccttttgt tcattctcaa 3121 attaatatta tttgtttttt ctcttatttg ttgtgtgttg aatttgaaat tataagagat 3181 atgcaaacat tttgttttga gtaaaaatgt gtcaaatcgt ggcctctaat gaccgaagtt 3241 aatatgagga gtaaaacact gaagcctgca ggcatgcaag ctgatccact agaggccatg 3301 gcggccgcac tagtggatcc cccgggatgc atgaattcaa gcttatcgat gtcgacaggc 3361 cttaagggcc agatcttggg cccggtaccc gatcagattg tcgtttcccg ccttcggttt 3421 aaactatcag tgtttgacag gatatattgg cgggtaaacc taagagaaaa gagcgtttat 3481 tagaataatc ggatatttaa aagggcgtga aaaggtttat ccgttcgtcc atttgtatgt 3541 gcatgccaac cacagggttc ccctcgggag tgcttggcat tccgtgcgat aatgacttct 3601 gttcaaccac ccaaacgtcg gaaagcctga cgacggagca gcattccaaa aagatccctt 3661 ggctcgtctg ggtcggctag aaggtcgagt gggctgctgt ggcttgatcc ctcaacgcgg 3721 tcgcggacgt agcgcagcgc cgaaaaatcc tcgatcgcaa atccgacgct gtcgaaaagc 3781 gtgatctgct tgtcgctctt tcggccgacg tcctggccag tcatcacgcg ccaaagttcc 3841 gtcacaggat gatctggcgc gagttgctgg atctcgcctt caatccgggt ctgtggcggg 3901 aactccacga aaatatccga acgcagcaag atatcgcggt gcatctcggt cttgcctggg 3961 cagtcgccgc cgacgccgtt gatgtggacg ccgaaaagga tctaggtgaa gatccttttt 4021 gataatctca tgaccaaaat cccttaacgt gagttttcgt tccactgagc gtcagacccc 4081 gtagaaaaga tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg 4141 caaacaaaaa aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact 4201 ctttttccga aggtaactgg cttcagcaga gcgcagatac caaatactgt tcttctagtg 4261 tagccgtagt taggccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg 4321 ctaatcctgt taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac 4381 tcaagacgat agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca 4441 cagcccagct tggagcgaac gacctacacc gaactgagat acctacagcg tgagctatga 4501 gaaagcgcca cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc 4561 ggaacaggag agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct 4621 gtcgggtttc gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg 4681 agcctatgga aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct 4741 tttgctcaca tgttctttcc tgcgttatcc cctgattctg tggataaccg attaccgcct 4801 ttgagtgagc tgataccgct cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg 4861 aggaagcgga agagcgcctg atgcggtatt ttctccttac gcatctgtgc ggtatttcac 4921 accgcatatg gtgcactctc agtacaatct gctctgatgc cgcatagtta agccagtata 4981 cactccgcta tcgctacgtg actgggtcat ggctgcgccc cgacacccgc caacacccgc 5041 tgacgcgccc tgacgggctt gtctgctccc ggcatccgct tacagacaag ctgtgaccgt 5101 ctccgggagc tgcatgtgtc agaggttttc accgtcatca ccgaaacgcg cgaggcaggg 5161 gtacgtcgag gtcgatccaa cccctccgct gctatagtgc agtcggcttc tgacgttcag 5221 tgcagccgtc ttctgaaaac gacatgtcgc acaagtccta agttacgcga caggctgccg 5281 ccctgccctt ttcctggcgt tttcttgtcg cgtgttttag tcgcataaag tagaatactt 5341 gcgactagaa ccggagacat tacgccatga acaagagcgc cgccgctggc ctgctgggct 5401 atgcccgcgt cagcaccgac gaccaggact tgaccaacca acgggccgaa ctgcacgcgg 5461 ccggctgcac caagctgttt tccgagaaga tcaccggcac caggcgcgac cgcccggagc 5521 tggccaggat gcttgaccac ctacgccctg gcgacgttgt gacagtgacc aggctagacc 5581 gcctggcccg cagcacccgc gacctactgg acattgccga gcgcatccag gaggccggcg 5641 cgggcctgcg tagcctggca gagccgtggg ccgacaccac cacgccggcc ggccgcatgg 5701 tgttgaccgt gttcgccggc attgccgagt tcgagcgttc cctaatcatc gaccgcaccc 5761 ggagcgggcg cgaggccgcc aaggcgcgag gcgtgaagtt tggcccccgc cctaccctca 5821 ccccggcaca gatcgcgcac gcccgcgagc tgatcgacca ggaaggccgc accgtgaaag 5881 aggcggctgc actgcttggc gtgcatcgct cgaccctgta ccgcgcactt gagcgcagcg 5941 aggaagtgac gcccaccgag gccaggcggc gcggtgcctt ccgtgaggac gcattgaccg 6001 aggccgacgc cctggcggcc gccgagaatg aacgccaaga ggaacaagca tgaaaccgca 6061 ccaggacggc caggacgaac cgtttttcat taccgaagag atcgaggcgg agatgatcgc 6121 ggccgggtac gtgttcgagc cgcccgcgca cgtctcaacc gtgcggctgc atgaaatcct 6181 ggccggtttg tctgatgcca agctcgcggc ctggccggcg agcttggccg ctgaagaaac 6241 cgagcgccgc cgtctaaaaa ggtgatgtgt atttgagtaa aacagcttgc gtcatgcggt 6301 cgctgcgtat atgatgcgat gagtaaataa acaaatacgc aaggggaacg catgaaggtt 6361 atcgctgtac ttaaccagaa aggcgggtca ggcaagacga ccatcgcaac ccatctagcc 6421 cgcgccctgc aactcgccgg ggccgatgtt ctgttagtcg attccgatcc ccagggcagt 6481 gcccgcgatt gggcggccgt gcgggaagat caaccgctaa ccgttgtcgg catcgaccgc 6541 ccgacgattg accgcgacgt gaaggccatc ggccggcgcg acttcgtagt gatcgacgga 6601 gcgccccagg cggcggactt ggctgtgtcc gcgatcaagg cagccgactt cgtgctgatt 6661 ccggtgcagc caagccctta cgacatatgg gccaccgccg acctggtgga gctggttaag 6721 cagcgcattg aggtcacgga tggaaggcta caagcggcct ttgtcgtgtc gcgggcgatc 6781 aaaggcacgc gcatcggcgg tgaggttgcc gaggcgctgg ccgggtacga gctgcccatt 6841 cttgagtccc gtatcacgca gcgcgtgagc tacccaggca ctgccgccgc cggcacaacc 6901 gttcttgaat cagaacccga gggcgacgct gcccgcgagg tccaggcgct ggccgctgaa 6961 attaaatcaa aactcatttg agttaatgag gtaaagagaa aatgagcaaa agcacaaaca 7021 cgctaagtgc cggccgtccg agcgcacgca gcagcaaggc tgcaacgttg gccagcctgg 7081 cagacacgcc agccatgaag cgggtcaact ttcagttgcc ggcggaggat cacaccaagc 7141 tgaagatgta cgcggtacgc caaggcaaga ccattaccga gctgctatct gaatacatcg 7201 cgcagctacc agagtaaatg agcaaatgaa taaatgagta gatgaatttt agcggctaaa 7261 ggaggcggca tggaaaatca agaacaacca ggcaccgacg ccgtggaatg ccccatgtgt 7321 ggaggaacgg gcggttggcc aggcgtaagc ggctgggttg tctgccggcc ctgcaatggc 7381 actggaaccc ccaagcccga ggaatcggcg tgagcggtcg caaaccatcc ggcccggtac 7441 aaatcggcgc ggcgctgggt gatgacctgg tggagaagtt gaaggcggcg caggccgccc 7501 agcggcaacg catcgaggca gaagcacgcc ccggtgaatc gtggcaagcg gccgctgatc 7561 gaatccgcaa agaatcccgg caaccgccgg cagccggtgc gccgtcgatt aggaagccgc 7621 ccaagggcga cgagcaacca gattttttcg ttccgatgct ctatgacgtg ggcacccgcg 7681 atagtcgcag catcatggac gtggccgttt tccgtctgtc gaagcgtgac cgacgagctg 7741 gcgaggtgat ccgctacgag cttccagacg ggcacgtaga ggtttccgca gggccggccg 7801 gcatggcgag tgtgtgggat tacgacctgg tactgatggc ggtttcccat ctaaccgaat 7861 ccatgaaccg ataccgggaa gggaagggag acaagcccgg ccgcgtgttc cgtccacacg 7921 ttgcggacgt actcaagttc tgccggcgag ccgatggcgg aaagcagaaa gacgacctgg 7981 tagaaacctg cattcggtta aacaccacgc acgttgccat gcagcgtacg aagaaggcca 8041 agaacggccg cctggtgacg gtatccgagg gtgaagcctt gattagccgc tacaagatcg 8101 taaagagcga aaccgggcgg ccggagtaca tcgagatcga gctagctgat tggatgtacc 8161 gcgagatcac agaaggcaag aacccggacg tgctgacggt tcaccccgat tactttttga 8221 tcgatcccgg catcggccgt tttctctacc gcctggcacg ccgcgccgca ggcaaggcag 8281 aagccagatg gttgttcaag acgatctacg aacgcagtgg cagcgccgga gagttcaaga 8341 agttctgttt caccgtgcgc aagctgatcg ggtcaaatga cctgccggag tacgatttga 8401 aggaggaggc ggggcaggct ggcccgatcc tagtcatgcg ctaccgcaac ctgatcgagg 8461 gcgaagcatc cgccggttcc taatgtacgg agcagatgct agggcaaatt gccctagcag 8521 gggaaaaagg tcgaaaaggt ctctttcctg tggatagcac gtacattggg aacccaaagc 8581 cgtacattgg gaaccggaac ccgtacattg ggaacccaaa gccgtacatt gggaaccggt 8641 cacacatgta agtgactgat ataaaagaga aaaaaggcga tttttccgcc taaaactctt 8701 taaaacttat taaaactctt aaaacccgcc tggcctgtgc ataactgtct ggccagcgca 8761 cagccgaaga gctgcaaaaa gcgcctaccc ttcggtcgct gcgctcccta cgccccgccg 8821 cttcgcgtcg gcctatcgcg gccgctggcc gctcaaaaat ggctggccta cggccaggca 8881 atctaccagg gcgcggacaa gccgcgccgt cgccactcga ccgccggcgc ccacatcaag 8941 gcaccggtgg gtatgcctga cgatgcgtgg agaccgaaac cttgcgctcg ttcgccagcc 9001 aggacagaaa tgcctcgact tcgctgctgc ccaaggttgc cgggtgacgc acaccgtgga 9061 aacggatgaa ggcacgaacc cagtggacat aagcctgttc ggttcgtaag ctgtaatgca 9121 agtagcgtat gcgctcacgc aactggtcca gaaccttgac cgaacgcagc ggtggtaacg 9181 gcgcagtggc ggttttcatg gcttgttatg actgtttttt tggggtacag tctatgcctc 9241 gggcatccaa gcagcaagcg cgttacgccg tgggtcgatg tttgatgtta tggagcagca 9301 acgatgttac gcagcagggc agtcgcccta aaacaaagtt aaacatcatg agggaagcgg 9361 tgatcgccga agtatcgact caactatcag aggtagttgg cgtcatcgag cgccatctcg 9421 aaccgacgtt gctggccgta catttgtacg gctccgcagt ggatggcggc ctgaagccac 9481 acagtgatat tgatttgctg gttacggtga ccgtaaggct tgatgaaaca acgcggcgag 9541 ctttgatcaa cgaccttttg gaaacttcgg cttcccctgg agagagcgag attctccgcg 9601 ctgtagaagt caccattgtt gtgcacgacg acatcattcc gtggcgttat ccagctaagc 9661 gcgaactgca atttggagaa tggcagcgca atgacattct tgcaggtatc ttcgagccag 9721 ccacgatcga cattgatctg gctatcttgc tgacaaaagc aagagaacat agcgttgcct 9781 tggtaggtcc agcggcggag gaactctttg atccggttcc tgaacaggat ctatttgagg 9841 cgctaaatga aaccttaacg ctatggaact cgccgcccga ctgggctggc gatgagcgaa 9901 atgtagtgct tacgttgtcc cgcatttggt acagcgcagt aaccggcaaa atcgcgccga 9961 aggatgtcgc tgccgactgg gcaatggagc gcctgccggc ccagtatcag cccgtcatac 10021 ttgaagctag acaggcttat cttggacaag aagaagatcg cttggcctcg cgcgcagatc 10081 agttggaaga atttgtccac tacgtgaaag gcgagatcac caaggtagtc ggcaaataat 10141 gtctaacaat tcgttcaagc cgacgccgct tcgcggcgcg gcttaactca agcgttagat 10201 gcactaagca cataattgct cacagccaaa ctatcaggtc aagtctgctt ttattatttt 10261 taagcgtgca taataagccc tacacaaatt gggagatata tcatgaaagg ctggcttttt 10321 cttgttatcg caatagttgg cgaagtaatc gcaacatagc ttgcttggtc gttccgcgtg 10381 aacgtcggct cgattgtacc tgcgttcaaa tactttgcga tcgtgttgcg cgcctgcccg 10441 gtgcgtcggc tgatctcacg gatcgactgc ttctctcgca acgccatccg acggatgatg 10501 tttaaaagtc ccatgtggat cactccgttg ccccgtcgct caccgtgttg gggggaaggt 10561 gcacatggct cagttctcaa tggaaattat ctgcctaacc ggctcagttc tgcgtagaaa 10621 ccaacatgca agctccaccg ggtgcaaagc ggcagcggc //