LOCUS B489p7oAct-LH 10187 bp DNA circular SYN 22-JAN-2016 DEFINITION Ligation of A373p6ido-Act* into B474p7oM-LH* ACCESSION B489p7oAct-LH KEYWORDS . SOURCE Unknown. ORGANISM Unknown Unclassified. REFERENCE 1 (bases 1 to 10187) AUTHORS Self JOURNAL Unpublished. COMMENT SECID/File created by Clone Manager, Scientific & Educational Software COMMENT SECNOTES|Vector molecule: B474p7oM-LH* Fragment ends: XhoI and XbaI Fragment size: 8770 Insert molecule: A373p6ido-Act* Fragment ends: XbaI and XhoI Fragment size: 1417 GelR Sequencing primers Act1 CACTATCACCGTGAGTTGTC FEATURES Location/Qualifiers misc_feature 1..24 /gene="LB" /product="Left Border" /SECDrawAs="Region" /SECStyleId=1 misc_feature 313..1710 /gene="ACT" /product="Actin Promotor" /SECDrawAs="Region" /SECStyleId=1 CDS 1738..1971 /gene="bar" /SECDrawAs="Gene" /SECStyleId=1 misc_feature 1972..2160 /gene="int" /product="STLS1" /SECDrawAs="Gene" /SECStyleId=1 /SECName="int" /SECDescr="STLS1" CDS 2161..2478 /gene="bar" /SECDrawAs="Gene" /SECStyleId=1 misc_feature 2498..2788 /gene="TE9" /product="Terminator Rbcs-E9" /SECDrawAs="Region" /SECStyleId=1 misc_feature 2964..2987 /gene="RB" /product="Right Border" /SECDrawAs="Region" /SECStyleId=1 misc_feature 3522..4219 /gene="ColE1" /product="ColE1 ORI" /SECDrawAs="Region" /SECStyleId=1 misc_feature 4768..8473 /gene="pVS1" /product="origin of replication for agrobacteria" /SECDrawAs="Region" /SECStyleId=1 CDS 8657..9667 /gene="Sm/Sp" /product="Strep/Spec resistence" /SECDrawAs="Gene" /SECStyleId=1 ORIGIN 1 ggcaggatat attcaattgt aaatggcttc atgtccggga aatctacatg gatcagcaat 61 gagtatgatg gtcaatatgg agaaaaagaa agagtaatta ccaatttttt ttcaattcaa 121 aaatgtagat gtccgcagcg ttattataaa atgaaagtac attttgataa aacgacaaat 181 tacgatccgt cgtatttata ggcgaaagca ataaacaaat tattctaatt cggaaatctt 241 tatttcgacg tgtctacatt cacgtccaaa tgggggctta gatgagaaac ttcacgatcg 301 gctctagaca ggtcgaggtc attcatatgc ttgagaagag agtcgggata gtccaaaata 361 aaacaaaggt aagattacct ggtcaaaagt gaaaacatca gttaaaaggt ggtataaagt 421 aaaatatcgg taataaaagg tggcccaaag tgaaatttac tcttttctac tattataaaa 481 attgaggatg tttttgtcgg tactttgata cgtcattttt gtatgaattg gtttttaagt 541 ttattcgctt ttggaaatgc atatctgtat ttgagtcggg ttttaagttc gtttgctttt 601 gtaaatacag agggatttgt ataagaaata tctttaaaaa aacccatatg ctaatttgac 661 ataatttttg agaaaaatat atattcaggc gaattctcac aatgaacaat aataagatta 721 aaatagcttt cccccgttgc agcgcatggg tattttttct agtaaaaata aaagataaac 781 ttagactcaa aacatttaca aaaacaaccc ctaaagttcc taaagcccaa agtgctatcc 841 acgatccata gcaagcccag cccaacccaa cccaacccaa cccaccccag tccagccaac 901 tggacaatag tctccacacc cccccactat caccgtgagt tgtccgcacg caccgcacgt 961 ctcgcagcca aaaaaaaaaa aagaaagaaa aaaaagaaaa agaaaaaaca gcaggtgggt 1021 ccgggtcgtg ggggccggaa acgcgaggag gatcgcgagc cagcgacgag gccggccctc 1081 cctccgcttc caaagaaacg ccccccatcg ccactatata catacccccc cctctcctcc 1141 catcccccca accctaccac caccaccacc accacctcca cctcctcccc cctcgctgcc 1201 ggacgacgag ctcctccccc ctccccctcc gccgccgccg cgccggtaac caccccgccc 1261 ctctcctctt tctttctccg tttttttttt ccgtctcggt ctcgatcttt ggccttggta 1321 gtttgggtgg gcgagaggcg gcttcgtgcg cgcccagatc ggtgcgcggg aggggcggga 1381 tctcgcggct ggggctctcg ccggcgtgga tccggcccgg atctcgcggg gaatggggct 1441 ctcggatgta gatctgcgat ccgccgttgt tgggggagat gatggggggt ttaaaatttc 1501 cgccatgcta aacaagatca ggaagagggg aaaagggcac tatggtttat atttttatat 1561 atttctgctg cttcgtcagg cttagatgtg ctagatcttt ctttcttctt tttgtgggta 1621 gaatttgaat ccctcagcat tgttcatcgg tagtttttct tttcatgatt tgtgacaaat 1681 gcagcctcgt gcggagcttt tttgtaggta ctaggctagc tcgagtcctc caccgaaatg 1741 ggccccgagc gcaggcccgc cgacatccgc cgtgccaccg aggccgacat gccagccgtc 1801 tgcaccatcg tgaaccacta catcgagacc tccacggtca acttccgcac agagccgcag 1861 gagcctcagg agtggaccga cgacctcgtg aggctgcgcg agcgctaccc ctggctcgtc 1921 gctgaggtgg atggcgaggt cgccggcatc gcctacgcgg gcccctggaa ggtaagtttc 1981 tgcttctacc tttgatatat atataataat tatcattaat tagtagtaat ataatatttc 2041 aaatattttt ttcaaaataa aagaatgtag tatatagcaa ttgcttttct gtagtttata 2101 agtgtgtata ttttaattta taacttttct aatatatgac caaaatttgt tgatgtgcag 2161 gcacgcaacg cctacgactg gacggcggag agcaccgtct acgtgtcccc acgccaccag 2221 cgcaccggcc tgggctccac gctctacacc cacctgctca agagcctgga ggcccagggc 2281 ttcaagtccg tggtcgctgt gatcggcctc cccaacgacc cgagcgtccg tatgcacgag 2341 gccctcggct acgcgccccg cgggatgctg agggccgcgg gcttcaagca cggcaactgg 2401 cacgacgtgg gcttctggca gctcgacttc tccctgcctg tcccaccccg cccggtgctc 2461 cccgtcaccg aaatctgaac tccggaatcg cgttcgagta ttatggcatt gggaaaactg 2521 tttttcttgt accatttgtt gtgcttgtaa tttactgtgt tttttattcg gttttcgcta 2581 tcgaactgtg aaatggaaat ggatggagaa gagttaatga atgatatggt ccttttgttc 2641 attctcaaat taatattatt tgttttttct cttatttgtt gtgtgttgaa tttgaaatta 2701 taagagatat gcaaacattt tgttttgagt aaaaatgtgt caaatcgtgg cctctaatga 2761 ccgaagttaa tatgaggagt aaaacactga agcctgcagg catgcaagct gatccactag 2821 aggccatggc ggccgcacta gtggatcccc cgggatgcat gaattcaagc ttatcgatgt 2881 cgacaggcct taagggccag atcttgggcc cggtacccga tcagattgtc gtttcccgcc 2941 ttcggtttaa actatcagtg tttgacagga tatattggcg ggtaaaccta agagaaaaga 3001 gcgtttatta gaataatcgg atatttaaaa gggcgtgaaa aggtttatcc gttcgtccat 3061 ttgtatgtgc atgccaacca cagggttccc ctcgggagtg cttggcattc cgtgcgataa 3121 tgacttctgt tcaaccaccc aaacgtcgga aagcctgacg acggagcagc attccaaaaa 3181 gatcccttgg ctcgtctggg tcggctagaa ggtcgagtgg gctgctgtgg cttgatccct 3241 caacgcggtc gcggacgtag cgcagcgccg aaaaatcctc gatcgcaaat ccgacgctgt 3301 cgaaaagcgt gatctgcttg tcgctctttc ggccgacgtc ctggccagtc atcacgcgcc 3361 aaagttccgt cacaggatga tctggcgcga gttgctggat ctcgccttca atccgggtct 3421 gtggcgggaa ctccacgaaa atatccgaac gcagcaagat atcgcggtgc atctcggtct 3481 tgcctgggca gtcgccgccg acgccgttga tgtggacgcc gaaaaggatc taggtgaaga 3541 tcctttttga taatctcatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt 3601 cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct 3661 gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc 3721 taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgttc 3781 ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc 3841 tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg 3901 ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt 3961 cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg 4021 agctatgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg 4081 gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt 4141 atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag 4201 gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt 4261 gctggccttt tgctcacatg ttctttcctg cgttatcccc tgattctgtg gataaccgat 4321 taccgccttt gagtgagctg ataccgctcg ccgcagccga acgaccgagc gcagcgagtc 4381 agtgagcgag gaagcggaag agcgcctgat gcggtatttt ctccttacgc atctgtgcgg 4441 tatttcacac cgcatatggt gcactctcag tacaatctgc tctgatgccg catagttaag 4501 ccagtataca ctccgctatc gctacgtgac tgggtcatgg ctgcgccccg acacccgcca 4561 acacccgctg acgcgccctg acgggcttgt ctgctcccgg catccgctta cagacaagct 4621 gtgaccgtct ccgggagctg catgtgtcag aggttttcac cgtcatcacc gaaacgcgcg 4681 aggcaggggt acgtcgaggt cgatccaacc cctccgctgc tatagtgcag tcggcttctg 4741 acgttcagtg cagccgtctt ctgaaaacga catgtcgcac aagtcctaag ttacgcgaca 4801 ggctgccgcc ctgccctttt cctggcgttt tcttgtcgcg tgttttagtc gcataaagta 4861 gaatacttgc gactagaacc ggagacatta cgccatgaac aagagcgccg ccgctggcct 4921 gctgggctat gcccgcgtca gcaccgacga ccaggacttg accaaccaac gggccgaact 4981 gcacgcggcc ggctgcacca agctgttttc cgagaagatc accggcacca ggcgcgaccg 5041 cccggagctg gccaggatgc ttgaccacct acgccctggc gacgttgtga cagtgaccag 5101 gctagaccgc ctggcccgca gcacccgcga cctactggac attgccgagc gcatccagga 5161 ggccggcgcg ggcctgcgta gcctggcaga gccgtgggcc gacaccacca cgccggccgg 5221 ccgcatggtg ttgaccgtgt tcgccggcat tgccgagttc gagcgttccc taatcatcga 5281 ccgcacccgg agcgggcgcg aggccgccaa ggcgcgaggc gtgaagtttg gcccccgccc 5341 taccctcacc ccggcacaga tcgcgcacgc ccgcgagctg atcgaccagg aaggccgcac 5401 cgtgaaagag gcggctgcac tgcttggcgt gcatcgctcg accctgtacc gcgcacttga 5461 gcgcagcgag gaagtgacgc ccaccgaggc caggcggcgc ggtgccttcc gtgaggacgc 5521 attgaccgag gccgacgccc tggcggccgc cgagaatgaa cgccaagagg aacaagcatg 5581 aaaccgcacc aggacggcca ggacgaaccg tttttcatta ccgaagagat cgaggcggag 5641 atgatcgcgg ccgggtacgt gttcgagccg cccgcgcacg tctcaaccgt gcggctgcat 5701 gaaatcctgg ccggtttgtc tgatgccaag ctcgcggcct ggccggcgag cttggccgct 5761 gaagaaaccg agcgccgccg tctaaaaagg tgatgtgtat ttgagtaaaa cagcttgcgt 5821 catgcggtcg ctgcgtatat gatgcgatga gtaaataaac aaatacgcaa ggggaacgca 5881 tgaaggttat cgctgtactt aaccagaaag gcgggtcagg caagacgacc atcgcaaccc 5941 atctagcccg cgccctgcaa ctcgccgggg ccgatgttct gttagtcgat tccgatcccc 6001 agggcagtgc ccgcgattgg gcggccgtgc gggaagatca accgctaacc gttgtcggca 6061 tcgaccgccc gacgattgac cgcgacgtga aggccatcgg ccggcgcgac ttcgtagtga 6121 tcgacggagc gccccaggcg gcggacttgg ctgtgtccgc gatcaaggca gccgacttcg 6181 tgctgattcc ggtgcagcca agcccttacg acatatgggc caccgccgac ctggtggagc 6241 tggttaagca gcgcattgag gtcacggatg gaaggctaca agcggccttt gtcgtgtcgc 6301 gggcgatcaa aggcacgcgc atcggcggtg aggttgccga ggcgctggcc gggtacgagc 6361 tgcccattct tgagtcccgt atcacgcagc gcgtgagcta cccaggcact gccgccgccg 6421 gcacaaccgt tcttgaatca gaacccgagg gcgacgctgc ccgcgaggtc caggcgctgg 6481 ccgctgaaat taaatcaaaa ctcatttgag ttaatgaggt aaagagaaaa tgagcaaaag 6541 cacaaacacg ctaagtgccg gccgtccgag cgcacgcagc agcaaggctg caacgttggc 6601 cagcctggca gacacgccag ccatgaagcg ggtcaacttt cagttgccgg cggaggatca 6661 caccaagctg aagatgtacg cggtacgcca aggcaagacc attaccgagc tgctatctga 6721 atacatcgcg cagctaccag agtaaatgag caaatgaata aatgagtaga tgaattttag 6781 cggctaaagg aggcggcatg gaaaatcaag aacaaccagg caccgacgcc gtggaatgcc 6841 ccatgtgtgg aggaacgggc ggttggccag gcgtaagcgg ctgggttgtc tgccggccct 6901 gcaatggcac tggaaccccc aagcccgagg aatcggcgtg agcggtcgca aaccatccgg 6961 cccggtacaa atcggcgcgg cgctgggtga tgacctggtg gagaagttga aggcggcgca 7021 ggccgcccag cggcaacgca tcgaggcaga agcacgcccc ggtgaatcgt ggcaagcggc 7081 cgctgatcga atccgcaaag aatcccggca accgccggca gccggtgcgc cgtcgattag 7141 gaagccgccc aagggcgacg agcaaccaga ttttttcgtt ccgatgctct atgacgtggg 7201 cacccgcgat agtcgcagca tcatggacgt ggccgttttc cgtctgtcga agcgtgaccg 7261 acgagctggc gaggtgatcc gctacgagct tccagacggg cacgtagagg tttccgcagg 7321 gccggccggc atggcgagtg tgtgggatta cgacctggta ctgatggcgg tttcccatct 7381 aaccgaatcc atgaaccgat accgggaagg gaagggagac aagcccggcc gcgtgttccg 7441 tccacacgtt gcggacgtac tcaagttctg ccggcgagcc gatggcggaa agcagaaaga 7501 cgacctggta gaaacctgca ttcggttaaa caccacgcac gttgccatgc agcgtacgaa 7561 gaaggccaag aacggccgcc tggtgacggt atccgagggt gaagccttga ttagccgcta 7621 caagatcgta aagagcgaaa ccgggcggcc ggagtacatc gagatcgagc tagctgattg 7681 gatgtaccgc gagatcacag aaggcaagaa cccggacgtg ctgacggttc accccgatta 7741 ctttttgatc gatcccggca tcggccgttt tctctaccgc ctggcacgcc gcgccgcagg 7801 caaggcagaa gccagatggt tgttcaagac gatctacgaa cgcagtggca gcgccggaga 7861 gttcaagaag ttctgtttca ccgtgcgcaa gctgatcggg tcaaatgacc tgccggagta 7921 cgatttgaag gaggaggcgg ggcaggctgg cccgatccta gtcatgcgct accgcaacct 7981 gatcgagggc gaagcatccg ccggttccta atgtacggag cagatgctag ggcaaattgc 8041 cctagcaggg gaaaaaggtc gaaaaggtct ctttcctgtg gatagcacgt acattgggaa 8101 cccaaagccg tacattggga accggaaccc gtacattggg aacccaaagc cgtacattgg 8161 gaaccggtca cacatgtaag tgactgatat aaaagagaaa aaaggcgatt tttccgccta 8221 aaactcttta aaacttatta aaactcttaa aacccgcctg gcctgtgcat aactgtctgg 8281 ccagcgcaca gccgaagagc tgcaaaaagc gcctaccctt cggtcgctgc gctccctacg 8341 ccccgccgct tcgcgtcggc ctatcgcggc cgctggccgc tcaaaaatgg ctggcctacg 8401 gccaggcaat ctaccagggc gcggacaagc cgcgccgtcg ccactcgacc gccggcgccc 8461 acatcaaggc accggtgggt atgcctgacg atgcgtggag accgaaacct tgcgctcgtt 8521 cgccagccag gacagaaatg cctcgacttc gctgctgccc aaggttgccg ggtgacgcac 8581 accgtggaaa cggatgaagg cacgaaccca gtggacataa gcctgttcgg ttcgtaagct 8641 gtaatgcaag tagcgtatgc gctcacgcaa ctggtccaga accttgaccg aacgcagcgg 8701 tggtaacggc gcagtggcgg ttttcatggc ttgttatgac tgtttttttg gggtacagtc 8761 tatgcctcgg gcatccaagc agcaagcgcg ttacgccgtg ggtcgatgtt tgatgttatg 8821 gagcagcaac gatgttacgc agcagggcag tcgccctaaa acaaagttaa acatcatgag 8881 ggaagcggtg atcgccgaag tatcgactca actatcagag gtagttggcg tcatcgagcg 8941 ccatctcgaa ccgacgttgc tggccgtaca tttgtacggc tccgcagtgg atggcggcct 9001 gaagccacac agtgatattg atttgctggt tacggtgacc gtaaggcttg atgaaacaac 9061 gcggcgagct ttgatcaacg accttttgga aacttcggct tcccctggag agagcgagat 9121 tctccgcgct gtagaagtca ccattgttgt gcacgacgac atcattccgt ggcgttatcc 9181 agctaagcgc gaactgcaat ttggagaatg gcagcgcaat gacattcttg caggtatctt 9241 cgagccagcc acgatcgaca ttgatctggc tatcttgctg acaaaagcaa gagaacatag 9301 cgttgccttg gtaggtccag cggcggagga actctttgat ccggttcctg aacaggatct 9361 atttgaggcg ctaaatgaaa ccttaacgct atggaactcg ccgcccgact gggctggcga 9421 tgagcgaaat gtagtgctta cgttgtcccg catttggtac agcgcagtaa ccggcaaaat 9481 cgcgccgaag gatgtcgctg ccgactgggc aatggagcgc ctgccggccc agtatcagcc 9541 cgtcatactt gaagctagac aggcttatct tggacaagaa gaagatcgct tggcctcgcg 9601 cgcagatcag ttggaagaat ttgtccacta cgtgaaaggc gagatcacca aggtagtcgg 9661 caaataatgt ctaacaattc gttcaagccg acgccgcttc gcggcgcggc ttaactcaag 9721 cgttagatgc actaagcaca taattgctca cagccaaact atcaggtcaa gtctgctttt 9781 attattttta agcgtgcata ataagcccta cacaaattgg gagatatatc atgaaaggct 9841 ggctttttct tgttatcgca atagttggcg aagtaatcgc aacatagctt gcttggtcgt 9901 tccgcgtgaa cgtcggctcg attgtacctg cgttcaaata ctttgcgatc gtgttgcgcg 9961 cctgcccggt gcgtcggctg atctcacgga tcgactgctt ctctcgcaac gccatccgac 10021 ggatgatgtt taaaagtccc atgtggatca ctccgttgcc ccgtcgctca ccgtgttggg 10081 gggaaggtgc acatggctca gttctcaatg gaaattatct gcctaaccgg ctcagttctg 10141 cgtagaaacc aacatgcaag ctccaccggg tgcaaagcgg cagcggc //