LOCUS       B621p5oAct-LH2         11203 bp    DNA     circular SYN 22-JAN-2016
DEFINITION  Ligation of OsCPTepsps* into B487p5oAct-LH*
ACCESSION   B621p5oAct-LH2
KEYWORDS    .
SOURCE      Unknown.
  ORGANISM  Unknown
            Unclassified.
REFERENCE   1  (bases 1 to 11203)
  AUTHORS   Self
  JOURNAL   Unpublished.
COMMENT     SECID/File created by Clone Manager, Scientific & Educational Software
COMMENT     SECNOTES|Vector molecule:  B487p5oAct-LH*
               Fragment ends: BspEI and XhoI
               Fragment size: 10767
            Insert molecule:  OsCPTepsps*
               Fragment ends: XhoI and BspEI
               Fragment size: 436
            Genesynthesis
            Sequencing primers	
            F3F	CAGCATTGTTCATCGGTAGT
FEATURES             Location/Qualifiers
     misc_feature    1..24
                     /gene="LB"
                     /product="Left Border"
                     /SECDrawAs="Region"
                     /SECStyleId=1
     misc_feature    313..1710
                     /gene="ACT"
                     /product="Actin Promotor"
                     /SECDrawAs="Region"
                     /SECStyleId=1
     CDS             1738..1950
                     /gene="cpt"
                     /product="CPT epsp Oryza sativa"
                     /SECDrawAs="Gene"
                     /SECStyleId=1
     CDS             1951..2472
                     /gene="EPSPS"
                     /codon_start=2
                     /translation="CTVPPAVRRPPGSLPASRGPSESPETRAFPTAHSCSAAWPAERQ
                     GSPAFWRVRMSSTPGRLCRLWEPASGRKATRGLSTAWETAVSSPQRHPWTSATPPPDA
                     V"
                     /SECDrawAs="Gene"
                     /SECStyleId=1
                     /SECName="epsps"
     misc_feature    2473..2661
                     /gene="int"
                     /product="Intron STLS1"
                     /SECDrawAs="Gene"
                     /SECStyleId=1
                     /SECName="int"
                     /SECDescr="Intron STLS1"
     CDS             2662..3504
                     /gene="epsps"
                     /codon_start=3
                     /SECDrawAs="Gene"
                     /SECStyleId=1
     misc_feature    3514..3804
                     /gene="TE9"
                     /product="Terminator Rbcs-E9"
                     /SECDrawAs="Region"
                     /SECStyleId=1
     misc_feature    3980..4003
                     /gene="RB"
                     /product="Right Border"
                     /SECDrawAs="Region"
                     /SECStyleId=1
     misc_feature    4538..5235
                     /gene="ColE1"
                     /product="ColE1 ORI"
                     /SECDrawAs="Region"
                     /SECStyleId=1
     misc_feature    5784..9489
                     /gene="pVS1"
                     /product="origin of replication for agrobacteria"
                     /SECDrawAs="Region"
                     /SECStyleId=1
     CDS             9673..10683
                     /gene="Sm/Sp"
                     /product="Strep/Spec resistence"
                     /SECDrawAs="Gene"
                     /SECStyleId=1
ORIGIN      
        1 ggcaggatat attcaattgt aaatggcttc atgtccggga aatctacatg gatcagcaat
       61 gagtatgatg gtcaatatgg agaaaaagaa agagtaatta ccaatttttt ttcaattcaa
      121 aaatgtagat gtccgcagcg ttattataaa atgaaagtac attttgataa aacgacaaat
      181 tacgatccgt cgtatttata ggcgaaagca ataaacaaat tattctaatt cggaaatctt
      241 tatttcgacg tgtctacatt cacgtccaaa tgggggctta gatgagaaac ttcacgatcg
      301 gctctagaca ggtcgaggtc attcatatgc ttgagaagag agtcgggata gtccaaaata
      361 aaacaaaggt aagattacct ggtcaaaagt gaaaacatca gttaaaaggt ggtataaagt
      421 aaaatatcgg taataaaagg tggcccaaag tgaaatttac tcttttctac tattataaaa
      481 attgaggatg tttttgtcgg tactttgata cgtcattttt gtatgaattg gtttttaagt
      541 ttattcgctt ttggaaatgc atatctgtat ttgagtcggg ttttaagttc gtttgctttt
      601 gtaaatacag agggatttgt ataagaaata tctttaaaaa aacccatatg ctaatttgac
      661 ataatttttg agaaaaatat atattcaggc gaattctcac aatgaacaat aataagatta
      721 aaatagcttt cccccgttgc agcgcatggg tattttttct agtaaaaata aaagataaac
      781 ttagactcaa aacatttaca aaaacaaccc ctaaagttcc taaagcccaa agtgctatcc
      841 acgatccata gcaagcccag cccaacccaa cccaacccaa cccaccccag tccagccaac
      901 tggacaatag tctccacacc cccccactat caccgtgagt tgtccgcacg caccgcacgt
      961 ctcgcagcca aaaaaaaaaa aagaaagaaa aaaaagaaaa agaaaaaaca gcaggtgggt
     1021 ccgggtcgtg ggggccggaa acgcgaggag gatcgcgagc cagcgacgag gccggccctc
     1081 cctccgcttc caaagaaacg ccccccatcg ccactatata catacccccc cctctcctcc
     1141 catcccccca accctaccac caccaccacc accacctcca cctcctcccc cctcgctgcc
     1201 ggacgacgag ctcctccccc ctccccctcc gccgccgccg cgccggtaac caccccgccc
     1261 ctctcctctt tctttctccg tttttttttt ccgtctcggt ctcgatcttt ggccttggta
     1321 gtttgggtgg gcgagaggcg gcttcgtgcg cgcccagatc ggtgcgcggg aggggcggga
     1381 tctcgcggct ggggctctcg ccggcgtgga tccggcccgg atctcgcggg gaatggggct
     1441 ctcggatgta gatctgcgat ccgccgttgt tgggggagat gatggggggt ttaaaatttc
     1501 cgccatgcta aacaagatca ggaagagggg aaaagggcac tatggtttat atttttatat
     1561 atttctgctg cttcgtcagg cttagatgtg ctagatcttt ctttcttctt tttgtgggta
     1621 gaatttgaat ccctcagcat tgttcatcgg tagtttttct tttcatgatt tgtgacaaat
     1681 gcagcctcgt gcggagcttt tttgtaggta ctaggctagc tcgagtcctc caccgaaatg
     1741 gcctccaacg ctgccgccgc agccgctgtc agcttggacc aagccgtggc ggcctctgct
     1801 gccttctcct cacgcaagca gctccgtctg cccgccgctg ccaggggtgg catgagagtt
     1861 cgcgtccgcg cccgtggaag gcgcgaggca gtggttgtcg ccagcgcttc cagctcgtcc
     1921 gtggccgcgc cagccgctaa ggccgaagag ttgcacggtg cctccagccg tccggcgacc
     1981 gccaggaagt cttccggcct ctcggggacc gtcagaatcc ccggagacaa gagcatttcc
     2041 caccgctcat tcatgttcgg cggcctggcc agcggagaga caaggatcac cggccttctg
     2101 gagggtgagg atgtcatcaa caccgggaag gctatgcagg ctatgggagc ccgcatccgg
     2161 aaggaaggcg acacgtggat tatcgacggc gtgggaaacg gcggtctcct cgccccagag
     2221 gcacccttgg acttcggcaa cgccgccacc ggatgccgtc tgactatggg gcttgttggc
     2281 gtgtacgatt tcgactccac cttcatcgga gacgcttccc tcaccaagcg ccctatgggc
     2341 agggtcctaa atccgctccg tgagatgggc gtgcaagtca agagcgagga cggagaccgc
     2401 ctgccagtta ccttgcgagg ccccaagact ccaacaccca tcacctacag ggtgcctatg
     2461 gcctctgcgc aggtaagttt ctgcttctac ctttgatata tatataataa ttatcattaa
     2521 ttagtagtaa tataatattt caaatatttt tttcaaaata aaagaatgta gtatatagca
     2581 attgcttttc tgtagtttat aagtgtgtat attttaattt ataacttttc taatatatga
     2641 ccaaaatttg ttgatgtgca ggtcaagtcc gccgtgctcc ttgccgggct gaacactccg
     2701 ggtattacca ccgtcatcga gcccattatg accagagatc ataccgagaa gatgctccag
     2761 ggattcggcg ctaacttgac ggttgaaacc gacgccgacg gcgtgcgcac catccgtctg
     2821 gagggaaggg gcaaactcac cggccaagtc atcgacgtgc caggagatcc cagctcgact
     2881 gcttttccac ttgtagccgc cctcttggtc cctggttccg acgtgaccat cctgaacgtt
     2941 cttatgaacc cgacccgcac cgggctcatc ttgactctcc aggagatggg cgctgacatc
     3001 gaagtcatta accccaggct ggccggaggc gaggacgtgg cagatttgcg cgtccgtagt
     3061 tccaccctga agggtgtgac agttccagag gaccgggctc ccagcatgat cgacgagtac
     3121 cctatccttg ccgtcgccgc ggccttcgcc gagggcgcta ccgtgatgaa cggattggag
     3181 gagctgcgcg tcaaggagtc agacagactc tccgccgtgg ccaatgggct caagctcaac
     3241 ggcgttgact gcgatgaggg agaaaccagc cttgtcgtgc gtggcaggcc ggacggcaag
     3301 ggactcggta acgcttctgg ggccgcagtc gccacgcacc tggaccaccg catcgcgatg
     3361 tccttcctcg tgatgggcct tgtttcggag aacccagtca ccgtggacga tgccaccatg
     3421 attgctacta gcttccccga gttcatggac ttgatggccg gactgggagc caagatcgag
     3481 ctgtccgaca ccaaggctgc ctaacgcgtt cgagtattat ggcattggga aaactgtttt
     3541 tcttgtacca tttgttgtgc ttgtaattta ctgtgttttt tattcggttt tcgctatcga
     3601 actgtgaaat ggaaatggat ggagaagagt taatgaatga tatggtcctt ttgttcattc
     3661 tcaaattaat attatttgtt ttttctctta tttgttgtgt gttgaatttg aaattataag
     3721 agatatgcaa acattttgtt ttgagtaaaa atgtgtcaaa tcgtggcctc taatgaccga
     3781 agttaatatg aggagtaaaa cactgaagcc tgcaggcatg caagctgatc cactagaggc
     3841 catggcggcc gcactagtgg atcccccggg atgcatgaat tcaagcttat cgatgtcgac
     3901 aggccttaag ggccagatct tgggcccggt acccgatcag attgtcgttt cccgccttcg
     3961 gtttaaacta tcagtgtttg acaggatata ttggcgggta aacctaagag aaaagagcgt
     4021 ttattagaat aatcggatat ttaaaagggc gtgaaaaggt ttatccgttc gtccatttgt
     4081 atgtgcatgc caaccacagg gttcccctcg ggagtgcttg gcattccgtg cgataatgac
     4141 ttctgttcaa ccacccaaac gtcggaaagc ctgacgacgg agcagcattc caaaaagatc
     4201 ccttggctcg tctgggtcgg ctagaaggtc gagtgggctg ctgtggcttg atccctcaac
     4261 gcggtcgcgg acgtagcgca gcgccgaaaa atcctcgatc gcaaatccga cgctgtcgaa
     4321 aagcgtgatc tgcttgtcgc tctttcggcc gacgtcctgg ccagtcatca cgcgccaaag
     4381 ttccgtcaca ggatgatctg gcgcgagttg ctggatctcg ccttcaatcc gggtctgtgg
     4441 cgggaactcc acgaaaatat ccgaacgcag caagatatcg cggtgcatct cggtcttgcc
     4501 tgggcagtcg ccgccgacgc cgttgatgtg gacgccgaaa aggatctagg tgaagatcct
     4561 ttttgataat ctcatgacca aaatccctta acgtgagttt tcgttccact gagcgtcaga
     4621 ccccgtagaa aagatcaaag gatcttcttg agatcctttt tttctgcgcg taatctgctg
     4681 cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc aagagctacc
     4741 aactcttttt ccgaaggtaa ctggcttcag cagagcgcag ataccaaata ctgttcttct
     4801 agtgtagccg tagttaggcc accacttcaa gaactctgta gcaccgccta catacctcgc
     4861 tctgctaatc ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt
     4921 ggactcaaga cgatagttac cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg
     4981 cacacagccc agcttggagc gaacgaccta caccgaactg agatacctac agcgtgagct
     5041 atgagaaagc gccacgcttc ccgaagggag aaaggcggac aggtatccgg taagcggcag
     5101 ggtcggaaca ggagagcgca cgagggagct tccaggggga aacgcctggt atctttatag
     5161 tcctgtcggg tttcgccacc tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg
     5221 gcggagccta tggaaaaacg ccagcaacgc ggccttttta cggttcctgg ccttttgctg
     5281 gccttttgct cacatgttct ttcctgcgtt atcccctgat tctgtggata accgattacc
     5341 gcctttgagt gagctgatac cgctcgccgc agccgaacga ccgagcgcag cgagtcagtg
     5401 agcgaggaag cggaagagcg cctgatgcgg tattttctcc ttacgcatct gtgcggtatt
     5461 tcacaccgca tatggtgcac tctcagtaca atctgctctg atgccgcata gttaagccag
     5521 tatacactcc gctatcgcta cgtgactggg tcatggctgc gccccgacac ccgccaacac
     5581 ccgctgacgc gccctgacgg gcttgtctgc tcccggcatc cgcttacaga caagctgtga
     5641 ccgtctccgg gagctgcatg tgtcagaggt tttcaccgtc atcaccgaaa cgcgcgaggc
     5701 aggggtacgt cgaggtcgat ccaacccctc cgctgctata gtgcagtcgg cttctgacgt
     5761 tcagtgcagc cgtcttctga aaacgacatg tcgcacaagt cctaagttac gcgacaggct
     5821 gccgccctgc ccttttcctg gcgttttctt gtcgcgtgtt ttagtcgcat aaagtagaat
     5881 acttgcgact agaaccggag acattacgcc atgaacaaga gcgccgccgc tggcctgctg
     5941 ggctatgccc gcgtcagcac cgacgaccag gacttgacca accaacgggc cgaactgcac
     6001 gcggccggct gcaccaagct gttttccgag aagatcaccg gcaccaggcg cgaccgcccg
     6061 gagctggcca ggatgcttga ccacctacgc cctggcgacg ttgtgacagt gaccaggcta
     6121 gaccgcctgg cccgcagcac ccgcgaccta ctggacattg ccgagcgcat ccaggaggcc
     6181 ggcgcgggcc tgcgtagcct ggcagagccg tgggccgaca ccaccacgcc ggccggccgc
     6241 atggtgttga ccgtgttcgc cggcattgcc gagttcgagc gttccctaat catcgaccgc
     6301 acccggagcg ggcgcgaggc cgccaaggcg cgaggcgtga agtttggccc ccgccctacc
     6361 ctcaccccgg cacagatcgc gcacgcccgc gagctgatcg accaggaagg ccgcaccgtg
     6421 aaagaggcgg ctgcactgct tggcgtgcat cgctcgaccc tgtaccgcgc acttgagcgc
     6481 agcgaggaag tgacgcccac cgaggccagg cggcgcggtg ccttccgtga ggacgcattg
     6541 accgaggccg acgccctggc ggccgccgag aatgaacgcc aagaggaaca agcatgaaac
     6601 cgcaccagga cggccaggac gaaccgtttt tcattaccga agagatcgag gcggagatga
     6661 tcgcggccgg gtacgtgttc gagccgcccg cgcacgtctc aaccgtgcgg ctgcatgaaa
     6721 tcctggccgg tttgtctgat gccaagctcg cggcctggcc ggcgagcttg gccgctgaag
     6781 aaaccgagcg ccgccgtcta aaaaggtgat gtgtatttga gtaaaacagc ttgcgtcatg
     6841 cggtcgctgc gtatatgatg cgatgagtaa ataaacaaat acgcaagggg aacgcatgaa
     6901 ggttatcgct gtacttaacc agaaaggcgg gtcaggcaag acgaccatcg caacccatct
     6961 agcccgcgcc ctgcaactcg ccggggccga tgttctgtta gtcgattccg atccccaggg
     7021 cagtgcccgc gattgggcgg ccgtgcggga agatcaaccg ctaaccgttg tcggcatcga
     7081 ccgcccgacg attgaccgcg acgtgaaggc catcggccgg cgcgacttcg tagtgatcga
     7141 cggagcgccc caggcggcgg acttggctgt gtccgcgatc aaggcagccg acttcgtgct
     7201 gattccggtg cagccaagcc cttacgacat atgggccacc gccgacctgg tggagctggt
     7261 taagcagcgc attgaggtca cggatggaag gctacaagcg gcctttgtcg tgtcgcgggc
     7321 gatcaaaggc acgcgcatcg gcggtgaggt tgccgaggcg ctggccgggt acgagctgcc
     7381 cattcttgag tcccgtatca cgcagcgcgt gagctaccca ggcactgccg ccgccggcac
     7441 aaccgttctt gaatcagaac ccgagggcga cgctgcccgc gaggtccagg cgctggccgc
     7501 tgaaattaaa tcaaaactca tttgagttaa tgaggtaaag agaaaatgag caaaagcaca
     7561 aacacgctaa gtgccggccg tccgagcgca cgcagcagca aggctgcaac gttggccagc
     7621 ctggcagaca cgccagccat gaagcgggtc aactttcagt tgccggcgga ggatcacacc
     7681 aagctgaaga tgtacgcggt acgccaaggc aagaccatta ccgagctgct atctgaatac
     7741 atcgcgcagc taccagagta aatgagcaaa tgaataaatg agtagatgaa ttttagcggc
     7801 taaaggaggc ggcatggaaa atcaagaaca accaggcacc gacgccgtgg aatgccccat
     7861 gtgtggagga acgggcggtt ggccaggcgt aagcggctgg gttgtctgcc ggccctgcaa
     7921 tggcactgga acccccaagc ccgaggaatc ggcgtgagcg gtcgcaaacc atccggcccg
     7981 gtacaaatcg gcgcggcgct gggtgatgac ctggtggaga agttgaaggc ggcgcaggcc
     8041 gcccagcggc aacgcatcga ggcagaagca cgccccggtg aatcgtggca agcggccgct
     8101 gatcgaatcc gcaaagaatc ccggcaaccg ccggcagccg gtgcgccgtc gattaggaag
     8161 ccgcccaagg gcgacgagca accagatttt ttcgttccga tgctctatga cgtgggcacc
     8221 cgcgatagtc gcagcatcat ggacgtggcc gttttccgtc tgtcgaagcg tgaccgacga
     8281 gctggcgagg tgatccgcta cgagcttcca gacgggcacg tagaggtttc cgcagggccg
     8341 gccggcatgg cgagtgtgtg ggattacgac ctggtactga tggcggtttc ccatctaacc
     8401 gaatccatga accgataccg ggaagggaag ggagacaagc ccggccgcgt gttccgtcca
     8461 cacgttgcgg acgtactcaa gttctgccgg cgagccgatg gcggaaagca gaaagacgac
     8521 ctggtagaaa cctgcattcg gttaaacacc acgcacgttg ccatgcagcg tacgaagaag
     8581 gccaagaacg gccgcctggt gacggtatcc gagggtgaag ccttgattag ccgctacaag
     8641 atcgtaaaga gcgaaaccgg gcggccggag tacatcgaga tcgagctagc tgattggatg
     8701 taccgcgaga tcacagaagg caagaacccg gacgtgctga cggttcaccc cgattacttt
     8761 ttgatcgatc ccggcatcgg ccgttttctc taccgcctgg cacgccgcgc cgcaggcaag
     8821 gcagaagcca gatggttgtt caagacgatc tacgaacgca gtggcagcgc cggagagttc
     8881 aagaagttct gtttcaccgt gcgcaagctg atcgggtcaa atgacctgcc ggagtacgat
     8941 ttgaaggagg aggcggggca ggctggcccg atcctagtca tgcgctaccg caacctgatc
     9001 gagggcgaag catccgccgg ttcctaatgt acggagcaga tgctagggca aattgcccta
     9061 gcaggggaaa aaggtcgaaa aggtctcttt cctgtggata gcacgtacat tgggaaccca
     9121 aagccgtaca ttgggaaccg gaacccgtac attgggaacc caaagccgta cattgggaac
     9181 cggtcacaca tgtaagtgac tgatataaaa gagaaaaaag gcgatttttc cgcctaaaac
     9241 tctttaaaac ttattaaaac tcttaaaacc cgcctggcct gtgcataact gtctggccag
     9301 cgcacagccg aagagctgca aaaagcgcct acccttcggt cgctgcgctc cctacgcccc
     9361 gccgcttcgc gtcggcctat cgcggccgct ggccgctcaa aaatggctgg cctacggcca
     9421 ggcaatctac cagggcgcgg acaagccgcg ccgtcgccac tcgaccgccg gcgcccacat
     9481 caaggcaccg gtgggtatgc ctgacgatgc gtggagaccg aaaccttgcg ctcgttcgcc
     9541 agccaggaca gaaatgcctc gacttcgctg ctgcccaagg ttgccgggtg acgcacaccg
     9601 tggaaacgga tgaaggcacg aacccagtgg acataagcct gttcggttcg taagctgtaa
     9661 tgcaagtagc gtatgcgctc acgcaactgg tccagaacct tgaccgaacg cagcggtggt
     9721 aacggcgcag tggcggtttt catggcttgt tatgactgtt tttttggggt acagtctatg
     9781 cctcgggcat ccaagcagca agcgcgttac gccgtgggtc gatgtttgat gttatggagc
     9841 agcaacgatg ttacgcagca gggcagtcgc cctaaaacaa agttaaacat catgagggaa
     9901 gcggtgatcg ccgaagtatc gactcaacta tcagaggtag ttggcgtcat cgagcgccat
     9961 ctcgaaccga cgttgctggc cgtacatttg tacggctccg cagtggatgg cggcctgaag
    10021 ccacacagtg atattgattt gctggttacg gtgaccgtaa ggcttgatga aacaacgcgg
    10081 cgagctttga tcaacgacct tttggaaact tcggcttccc ctggagagag cgagattctc
    10141 cgcgctgtag aagtcaccat tgttgtgcac gacgacatca ttccgtggcg ttatccagct
    10201 aagcgcgaac tgcaatttgg agaatggcag cgcaatgaca ttcttgcagg tatcttcgag
    10261 ccagccacga tcgacattga tctggctatc ttgctgacaa aagcaagaga acatagcgtt
    10321 gccttggtag gtccagcggc ggaggaactc tttgatccgg ttcctgaaca ggatctattt
    10381 gaggcgctaa atgaaacctt aacgctatgg aactcgccgc ccgactgggc tggcgatgag
    10441 cgaaatgtag tgcttacgtt gtcccgcatt tggtacagcg cagtaaccgg caaaatcgcg
    10501 ccgaaggatg tcgctgccga ctgggcaatg gagcgcctgc cggcccagta tcagcccgtc
    10561 atacttgaag ctagacaggc ttatcttgga caagaagaag atcgcttggc ctcgcgcgca
    10621 gatcagttgg aagaatttgt ccactacgtg aaaggcgaga tcaccaaggt agtcggcaaa
    10681 taatgtctaa caattcgttc aagccgacgc cgcttcgcgg cgcggcttaa ctcaagcgtt
    10741 agatgcacta agcacataat tgctcacagc caaactatca ggtcaagtct gcttttatta
    10801 tttttaagcg tgcataataa gccctacaca aattgggaga tatatcatga aaggctggct
    10861 ttttcttgtt atcgcaatag ttggcgaagt aatcgcaaca tagcttgctt ggtcgttccg
    10921 cgtgaacgtc ggctcgattg tacctgcgtt caaatacttt gcgatcgtgt tgcgcgcctg
    10981 cccggtgcgt cggctgatct cacggatcga ctgcttctct cgcaacgcca tccgacggat
    11041 gatgtttaaa agtcccatgt ggatcactcc gttgccccgt cgctcaccgt gttgggggga
    11101 aggtgcacat ggctcagttc tcaatggaaa ttatctgcct aaccggctca gttctgcgta
    11161 gaaaccaaca tgcaagctcc accgggtgca aagcggcagc ggc
//
