LOCUS       B479p7oUZm-LH          10287 bp    DNA     circular SYN 26-JAN-2016
DEFINITION  Ligation of UbiZm-Bsa* into B474p7oM-LH*
ACCESSION   B479p7oUZm-LH
KEYWORDS    .
SOURCE      Unknown.
  ORGANISM  Unknown
            Unclassified.
REFERENCE   1  (bases 1 to 10287)
  AUTHORS   Self
  JOURNAL   Unpublished.
COMMENT     SECID/File created by Clone Manager, Scientific & Educational Software
COMMENT     SECNOTES|Vector molecule:  B474p7oM-LH*
               Fragment ends: XhoI and XbaI
               Fragment size: 8770
            Insert molecule:  UbiZm-Bsa*
               Fragment ends: BsaI
               Fragment size: 1517
            Primer for gene synthesis
            BsaXba	ccGGTCTCtctagatCTGCAGTGCAGCGT
            BsaXho	ccGGTCTCctcgagGACCTGCAGAAGTAACAC
            Sequencing primers	
            LHX1	CTTTATTTCGACGTGTCTAC
            UF1	CGAGTAGATAATGCCAGCC
FEATURES             Location/Qualifiers
     CDS             62..1569
                     /gene="UbiZm"
                     /product="Promotor"
                     /SECDrawAs="Region"
                     /SECStyleId=1
                     /SECName="UbiZm"
                     /SECDescr="Promotor"
     misc_feature    1049..1568
                     /gene="int"
                     /product="Intron"
                     /SECDrawAs="Region"
                     /SECStyleId=1
                     /SECName="int"
                     /SECDescr="Intron"
     CDS             1590..1823
                     /gene="bar"
                     /SECDrawAs="Gene"
                     /SECStyleId=1
     misc_feature    1824..2012
                     /gene="int"
                     /product="STLS1"
                     /SECDrawAs="Gene"
                     /SECStyleId=1
                     /SECName="int"
                     /SECDescr="STLS1"
     CDS             2013..2330
                     /gene="int"
                     /SECDrawAs="Gene"
                     /SECStyleId=1
     misc_feature    2350..2640
                     /gene="TE9"
                     /product="Terminator Rbcs-E9"
                     /SECDrawAs="Region"
                     /SECStyleId=1
     misc_feature    2816..2839
                     /gene="RB"
                     /product="Right Border"
                     /SECDrawAs="Region"
                     /SECStyleId=1
     misc_feature    3374..4071
                     /gene="ColE1"
                     /product="ColE1 ORI"
                     /SECDrawAs="Region"
                     /SECStyleId=1
     misc_feature    4620..8325
                     /gene="pVS1"
                     /product="origin of replication for agrobacteria"
                     /SECDrawAs="Region"
                     /SECStyleId=1
     CDS             8509..9519
                     /gene="Sm/Sp"
                     /product="Strep/Spec resistence"
                     /SECDrawAs="Gene"
                     /SECStyleId=1
     misc_feature    10040..10063
                     /gene="LB"
                     /product="Left Border"
                     /SECDrawAs="Region"
                     /SECStyleId=1
ORIGIN      
        1 cgtgtctaca ttcacgtcca aatgggggct tagatgagaa acttcacgat cggctctaga
       61 tctgcagtgc agcgtgaccc ggtcgtgccc ctctctagag ataatgagca ttgcatgtct
      121 aagttataaa aaattaccac atattttttt tgtcacactt gtttgaagtg cagtttatct
      181 atctttatac atatatttaa actttactct acgaataata taatctatag tactacaata
      241 atatcagtgt tttagagaat catataaatg aacagttaga catggtctaa aggacaattg
      301 agtattttga caacaggact ctacagtttt atctttttag tgtgcatgtg ttctcctttt
      361 tttttgcaaa tagcttcacc tatataatac ttcatccatt ttattagtac atccatttag
      421 ggtttagggt taatggtttt tatagactaa tttttttagt acatctattt tattctattt
      481 tagcctctaa attaagaaaa ctaaaactct attttagttt ttttatttaa taatttagat
      541 ataaaataga ataaaataaa gtgactaaaa attaaacaaa taccctttaa gaaattaaaa
      601 aaactaagga aacatttttc ttgtttcgag tagataatgc cagcctgtta aacgccgtcg
      661 atcgacgagt ctaacggaca ccaaccagcg aaccagcagc gtcgcgtcgg gccaagcgaa
      721 gcagacggca cggcatctct gtcgctgcct ctggacccct ctcgagagtt ccgctccacc
      781 gttggacttg ctccgctgtc ggcatccaga aattgcgtgg cggagcggca gacgtgagcc
      841 ggcacggcag gcggcctcct cctcctctca cggcaccggc agctacgggg gattcctttc
      901 ccaccgctcc ttcgctttcc cttcctcgcc cgccgtaata aatagacacc ccctccacac
      961 cctctttccc caacctcgtg ttgttcggag cgcacacaca cacaaccaga tctcccccaa
     1021 atccacccgt cggcacctcc gcttcaaggt acgccgctcg tcctcccccc ccccccctct
     1081 ctaccttctc tagatcggcg ttccggtcca tggttagggc ccggtagttc tacttctgtt
     1141 catgtttgtg ttagatccgt gtttgtgtta gatccgtgct gctagcgttc gtacacggat
     1201 gcgacctgta cgtcagacac gttctgattg ctaacttgcc agtgtttctc tttggggaat
     1261 cctgggatgg ctctagccgt tccgcagacg ggatcgatct aggataggta tacatgttga
     1321 tgtgggtttt actgatgcat atacatgatg gcatatgcag catctattca tatgctctaa
     1381 ccttgagtac ctatctatta taataaacaa gtatgtttta taattatttt gatcttgata
     1441 tacttggatg atggcatatg cagcagctat atgtggattt ttttagccct gccttcatac
     1501 gctatttatt tgcttggtac tgtttctttt gtcgatgctc accctgttgt ttggtgttac
     1561 ttctgcaggt cctcgagtcc tccaccgaaa tgggccccga gcgcaggccc gccgacatcc
     1621 gccgtgccac cgaggccgac atgccagccg tctgcaccat cgtgaaccac tacatcgaga
     1681 cctccacggt caacttccgc acagagccgc aggagcctca ggagtggacc gacgacctcg
     1741 tgaggctgcg cgagcgctac ccctggctcg tcgctgaggt ggatggcgag gtcgccggca
     1801 tcgcctacgc gggcccctgg aaggtaagtt tctgcttcta cctttgatat atatataata
     1861 attatcatta attagtagta atataatatt tcaaatattt ttttcaaaat aaaagaatgt
     1921 agtatatagc aattgctttt ctgtagttta taagtgtgta tattttaatt tataactttt
     1981 ctaatatatg accaaaattt gttgatgtgc aggcacgcaa cgcctacgac tggacggcgg
     2041 agagcaccgt ctacgtgtcc ccacgccacc agcgcaccgg cctgggctcc acgctctaca
     2101 cccacctgct caagagcctg gaggcccagg gcttcaagtc cgtggtcgct gtgatcggcc
     2161 tccccaacga cccgagcgtc cgtatgcacg aggccctcgg ctacgcgccc cgcgggatgc
     2221 tgagggccgc gggcttcaag cacggcaact ggcacgacgt gggcttctgg cagctcgact
     2281 tctccctgcc tgtcccaccc cgcccggtgc tccccgtcac cgaaatctga actccggaat
     2341 cgcgttcgag tattatggca ttgggaaaac tgtttttctt gtaccatttg ttgtgcttgt
     2401 aatttactgt gttttttatt cggttttcgc tatcgaactg tgaaatggaa atggatggag
     2461 aagagttaat gaatgatatg gtccttttgt tcattctcaa attaatatta tttgtttttt
     2521 ctcttatttg ttgtgtgttg aatttgaaat tataagagat atgcaaacat tttgttttga
     2581 gtaaaaatgt gtcaaatcgt ggcctctaat gaccgaagtt aatatgagga gtaaaacact
     2641 gaagcctgca ggcatgcaag ctgatccact agaggccatg gcggccgcac tagtggatcc
     2701 cccgggatgc atgaattcaa gcttatcgat gtcgacaggc cttaagggcc agatcttggg
     2761 cccggtaccc gatcagattg tcgtttcccg ccttcggttt aaactatcag tgtttgacag
     2821 gatatattgg cgggtaaacc taagagaaaa gagcgtttat tagaataatc ggatatttaa
     2881 aagggcgtga aaaggtttat ccgttcgtcc atttgtatgt gcatgccaac cacagggttc
     2941 ccctcgggag tgcttggcat tccgtgcgat aatgacttct gttcaaccac ccaaacgtcg
     3001 gaaagcctga cgacggagca gcattccaaa aagatccctt ggctcgtctg ggtcggctag
     3061 aaggtcgagt gggctgctgt ggcttgatcc ctcaacgcgg tcgcggacgt agcgcagcgc
     3121 cgaaaaatcc tcgatcgcaa atccgacgct gtcgaaaagc gtgatctgct tgtcgctctt
     3181 tcggccgacg tcctggccag tcatcacgcg ccaaagttcc gtcacaggat gatctggcgc
     3241 gagttgctgg atctcgcctt caatccgggt ctgtggcggg aactccacga aaatatccga
     3301 acgcagcaag atatcgcggt gcatctcggt cttgcctggg cagtcgccgc cgacgccgtt
     3361 gatgtggacg ccgaaaagga tctaggtgaa gatccttttt gataatctca tgaccaaaat
     3421 cccttaacgt gagttttcgt tccactgagc gtcagacccc gtagaaaaga tcaaaggatc
     3481 ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct
     3541 accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga aggtaactgg
     3601 cttcagcaga gcgcagatac caaatactgt tcttctagtg tagccgtagt taggccacca
     3661 cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt taccagtggc
     3721 tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat agttaccgga
     3781 taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct tggagcgaac
     3841 gacctacacc gaactgagat acctacagcg tgagctatga gaaagcgcca cgcttcccga
     3901 agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag agcgcacgag
     3961 ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc gccacctctg
     4021 acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga aaaacgccag
     4081 caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca tgttctttcc
     4141 tgcgttatcc cctgattctg tggataaccg attaccgcct ttgagtgagc tgataccgct
     4201 cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg aggaagcgga agagcgcctg
     4261 atgcggtatt ttctccttac gcatctgtgc ggtatttcac accgcatatg gtgcactctc
     4321 agtacaatct gctctgatgc cgcatagtta agccagtata cactccgcta tcgctacgtg
     4381 actgggtcat ggctgcgccc cgacacccgc caacacccgc tgacgcgccc tgacgggctt
     4441 gtctgctccc ggcatccgct tacagacaag ctgtgaccgt ctccgggagc tgcatgtgtc
     4501 agaggttttc accgtcatca ccgaaacgcg cgaggcaggg gtacgtcgag gtcgatccaa
     4561 cccctccgct gctatagtgc agtcggcttc tgacgttcag tgcagccgtc ttctgaaaac
     4621 gacatgtcgc acaagtccta agttacgcga caggctgccg ccctgccctt ttcctggcgt
     4681 tttcttgtcg cgtgttttag tcgcataaag tagaatactt gcgactagaa ccggagacat
     4741 tacgccatga acaagagcgc cgccgctggc ctgctgggct atgcccgcgt cagcaccgac
     4801 gaccaggact tgaccaacca acgggccgaa ctgcacgcgg ccggctgcac caagctgttt
     4861 tccgagaaga tcaccggcac caggcgcgac cgcccggagc tggccaggat gcttgaccac
     4921 ctacgccctg gcgacgttgt gacagtgacc aggctagacc gcctggcccg cagcacccgc
     4981 gacctactgg acattgccga gcgcatccag gaggccggcg cgggcctgcg tagcctggca
     5041 gagccgtggg ccgacaccac cacgccggcc ggccgcatgg tgttgaccgt gttcgccggc
     5101 attgccgagt tcgagcgttc cctaatcatc gaccgcaccc ggagcgggcg cgaggccgcc
     5161 aaggcgcgag gcgtgaagtt tggcccccgc cctaccctca ccccggcaca gatcgcgcac
     5221 gcccgcgagc tgatcgacca ggaaggccgc accgtgaaag aggcggctgc actgcttggc
     5281 gtgcatcgct cgaccctgta ccgcgcactt gagcgcagcg aggaagtgac gcccaccgag
     5341 gccaggcggc gcggtgcctt ccgtgaggac gcattgaccg aggccgacgc cctggcggcc
     5401 gccgagaatg aacgccaaga ggaacaagca tgaaaccgca ccaggacggc caggacgaac
     5461 cgtttttcat taccgaagag atcgaggcgg agatgatcgc ggccgggtac gtgttcgagc
     5521 cgcccgcgca cgtctcaacc gtgcggctgc atgaaatcct ggccggtttg tctgatgcca
     5581 agctcgcggc ctggccggcg agcttggccg ctgaagaaac cgagcgccgc cgtctaaaaa
     5641 ggtgatgtgt atttgagtaa aacagcttgc gtcatgcggt cgctgcgtat atgatgcgat
     5701 gagtaaataa acaaatacgc aaggggaacg catgaaggtt atcgctgtac ttaaccagaa
     5761 aggcgggtca ggcaagacga ccatcgcaac ccatctagcc cgcgccctgc aactcgccgg
     5821 ggccgatgtt ctgttagtcg attccgatcc ccagggcagt gcccgcgatt gggcggccgt
     5881 gcgggaagat caaccgctaa ccgttgtcgg catcgaccgc ccgacgattg accgcgacgt
     5941 gaaggccatc ggccggcgcg acttcgtagt gatcgacgga gcgccccagg cggcggactt
     6001 ggctgtgtcc gcgatcaagg cagccgactt cgtgctgatt ccggtgcagc caagccctta
     6061 cgacatatgg gccaccgccg acctggtgga gctggttaag cagcgcattg aggtcacgga
     6121 tggaaggcta caagcggcct ttgtcgtgtc gcgggcgatc aaaggcacgc gcatcggcgg
     6181 tgaggttgcc gaggcgctgg ccgggtacga gctgcccatt cttgagtccc gtatcacgca
     6241 gcgcgtgagc tacccaggca ctgccgccgc cggcacaacc gttcttgaat cagaacccga
     6301 gggcgacgct gcccgcgagg tccaggcgct ggccgctgaa attaaatcaa aactcatttg
     6361 agttaatgag gtaaagagaa aatgagcaaa agcacaaaca cgctaagtgc cggccgtccg
     6421 agcgcacgca gcagcaaggc tgcaacgttg gccagcctgg cagacacgcc agccatgaag
     6481 cgggtcaact ttcagttgcc ggcggaggat cacaccaagc tgaagatgta cgcggtacgc
     6541 caaggcaaga ccattaccga gctgctatct gaatacatcg cgcagctacc agagtaaatg
     6601 agcaaatgaa taaatgagta gatgaatttt agcggctaaa ggaggcggca tggaaaatca
     6661 agaacaacca ggcaccgacg ccgtggaatg ccccatgtgt ggaggaacgg gcggttggcc
     6721 aggcgtaagc ggctgggttg tctgccggcc ctgcaatggc actggaaccc ccaagcccga
     6781 ggaatcggcg tgagcggtcg caaaccatcc ggcccggtac aaatcggcgc ggcgctgggt
     6841 gatgacctgg tggagaagtt gaaggcggcg caggccgccc agcggcaacg catcgaggca
     6901 gaagcacgcc ccggtgaatc gtggcaagcg gccgctgatc gaatccgcaa agaatcccgg
     6961 caaccgccgg cagccggtgc gccgtcgatt aggaagccgc ccaagggcga cgagcaacca
     7021 gattttttcg ttccgatgct ctatgacgtg ggcacccgcg atagtcgcag catcatggac
     7081 gtggccgttt tccgtctgtc gaagcgtgac cgacgagctg gcgaggtgat ccgctacgag
     7141 cttccagacg ggcacgtaga ggtttccgca gggccggccg gcatggcgag tgtgtgggat
     7201 tacgacctgg tactgatggc ggtttcccat ctaaccgaat ccatgaaccg ataccgggaa
     7261 gggaagggag acaagcccgg ccgcgtgttc cgtccacacg ttgcggacgt actcaagttc
     7321 tgccggcgag ccgatggcgg aaagcagaaa gacgacctgg tagaaacctg cattcggtta
     7381 aacaccacgc acgttgccat gcagcgtacg aagaaggcca agaacggccg cctggtgacg
     7441 gtatccgagg gtgaagcctt gattagccgc tacaagatcg taaagagcga aaccgggcgg
     7501 ccggagtaca tcgagatcga gctagctgat tggatgtacc gcgagatcac agaaggcaag
     7561 aacccggacg tgctgacggt tcaccccgat tactttttga tcgatcccgg catcggccgt
     7621 tttctctacc gcctggcacg ccgcgccgca ggcaaggcag aagccagatg gttgttcaag
     7681 acgatctacg aacgcagtgg cagcgccgga gagttcaaga agttctgttt caccgtgcgc
     7741 aagctgatcg ggtcaaatga cctgccggag tacgatttga aggaggaggc ggggcaggct
     7801 ggcccgatcc tagtcatgcg ctaccgcaac ctgatcgagg gcgaagcatc cgccggttcc
     7861 taatgtacgg agcagatgct agggcaaatt gccctagcag gggaaaaagg tcgaaaaggt
     7921 ctctttcctg tggatagcac gtacattggg aacccaaagc cgtacattgg gaaccggaac
     7981 ccgtacattg ggaacccaaa gccgtacatt gggaaccggt cacacatgta agtgactgat
     8041 ataaaagaga aaaaaggcga tttttccgcc taaaactctt taaaacttat taaaactctt
     8101 aaaacccgcc tggcctgtgc ataactgtct ggccagcgca cagccgaaga gctgcaaaaa
     8161 gcgcctaccc ttcggtcgct gcgctcccta cgccccgccg cttcgcgtcg gcctatcgcg
     8221 gccgctggcc gctcaaaaat ggctggccta cggccaggca atctaccagg gcgcggacaa
     8281 gccgcgccgt cgccactcga ccgccggcgc ccacatcaag gcaccggtgg gtatgcctga
     8341 cgatgcgtgg agaccgaaac cttgcgctcg ttcgccagcc aggacagaaa tgcctcgact
     8401 tcgctgctgc ccaaggttgc cgggtgacgc acaccgtgga aacggatgaa ggcacgaacc
     8461 cagtggacat aagcctgttc ggttcgtaag ctgtaatgca agtagcgtat gcgctcacgc
     8521 aactggtcca gaaccttgac cgaacgcagc ggtggtaacg gcgcagtggc ggttttcatg
     8581 gcttgttatg actgtttttt tggggtacag tctatgcctc gggcatccaa gcagcaagcg
     8641 cgttacgccg tgggtcgatg tttgatgtta tggagcagca acgatgttac gcagcagggc
     8701 agtcgcccta aaacaaagtt aaacatcatg agggaagcgg tgatcgccga agtatcgact
     8761 caactatcag aggtagttgg cgtcatcgag cgccatctcg aaccgacgtt gctggccgta
     8821 catttgtacg gctccgcagt ggatggcggc ctgaagccac acagtgatat tgatttgctg
     8881 gttacggtga ccgtaaggct tgatgaaaca acgcggcgag ctttgatcaa cgaccttttg
     8941 gaaacttcgg cttcccctgg agagagcgag attctccgcg ctgtagaagt caccattgtt
     9001 gtgcacgacg acatcattcc gtggcgttat ccagctaagc gcgaactgca atttggagaa
     9061 tggcagcgca atgacattct tgcaggtatc ttcgagccag ccacgatcga cattgatctg
     9121 gctatcttgc tgacaaaagc aagagaacat agcgttgcct tggtaggtcc agcggcggag
     9181 gaactctttg atccggttcc tgaacaggat ctatttgagg cgctaaatga aaccttaacg
     9241 ctatggaact cgccgcccga ctgggctggc gatgagcgaa atgtagtgct tacgttgtcc
     9301 cgcatttggt acagcgcagt aaccggcaaa atcgcgccga aggatgtcgc tgccgactgg
     9361 gcaatggagc gcctgccggc ccagtatcag cccgtcatac ttgaagctag acaggcttat
     9421 cttggacaag aagaagatcg cttggcctcg cgcgcagatc agttggaaga atttgtccac
     9481 tacgtgaaag gcgagatcac caaggtagtc ggcaaataat gtctaacaat tcgttcaagc
     9541 cgacgccgct tcgcggcgcg gcttaactca agcgttagat gcactaagca cataattgct
     9601 cacagccaaa ctatcaggtc aagtctgctt ttattatttt taagcgtgca taataagccc
     9661 tacacaaatt gggagatata tcatgaaagg ctggcttttt cttgttatcg caatagttgg
     9721 cgaagtaatc gcaacatagc ttgcttggtc gttccgcgtg aacgtcggct cgattgtacc
     9781 tgcgttcaaa tactttgcga tcgtgttgcg cgcctgcccg gtgcgtcggc tgatctcacg
     9841 gatcgactgc ttctctcgca acgccatccg acggatgatg tttaaaagtc ccatgtggat
     9901 cactccgttg ccccgtcgct caccgtgttg gggggaaggt gcacatggct cagttctcaa
     9961 tggaaattat ctgcctaacc ggctcagttc tgcgtagaaa ccaacatgca agctccaccg
    10021 ggtgcaaagc ggcagcggcg gcaggatata ttcaattgta aatggcttca tgtccgggaa
    10081 atctacatgg atcagcaatg agtatgatgg tcaatatgga gaaaaagaaa gagtaattac
    10141 caattttttt tcaattcaaa aatgtagatg tccgcagcgt tattataaaa tgaaagtaca
    10201 ttttgataaa acgacaaatt acgatccgtc gtatttatag gcgaaagcaa taaacaaatt
    10261 attctaattc ggaaatcttt atttcga
//
