LOCUS B647pUbiZm-LRFP 5445 bp DNA circular SYN 08-FEB-2016 DEFINITION Ligation of TagRFP-Mlu-Sal* into B633pUbiZm-LGFP* ACCESSION B647pUbiZm-LRFP KEYWORDS . SOURCE Unknown. ORGANISM Unknown Unclassified. REFERENCE 1 (bases 1 to 5445) AUTHORS Self JOURNAL Unpublished. COMMENT SECID/File created by Clone Manager, Scientific & Educational Software COMMENT SECNOTES|Vector molecule: B633pUbiZm-LGFP* Fragment ends: SalI and MluI Fragment size: 4668 Insert molecule: TagRFP-Mlu-Sal* Fragment ends: MluI and SalI Fragment size: 777 Genesynthesis Sequencing primers Ubi1 CTGTTTCTTTTGTCGATGC FEATURES Location/Qualifiers misc_feature 106..1614 /gene="UbiZm" /product="Promotor" /SECDrawAs="Region" /SECStyleId=1 misc_feature 1093..1612 /gene="int" /product="Intron" /SECDrawAs="Region" /SECStyleId=1 misc_feature 1664..1714 /gene="link" /SECDrawAs="Region" /SECStyleId=1 CDS 1715..2428 /gene="rfp" /SECDrawAs="Gene" /SECStyleId=1 /SECName="rfp" misc_feature 2434..2629 /gene="Tocs" /product="Terminator" /SECDrawAs="Region" /SECStyleId=1 /SECName="Tocs" /SECDescr="Terminator" misc_feature 3445..4305 /gene="Amp" /product="ORF-1" /SECDrawAs="Gene" /SECStyleId=1 /SECName="Amp" /SECDescr="ORF-1" CDS 4348..5064 /gene="ColE1" /product="ColE1" /SECDrawAs="Region" /SECStyleId=1 /SECName="ColE1" /SECDescr="ColE1" ORIGIN 1 cacacaggaa acagctatga ccatgattac gccaagctcg aaattaaccc tcactaaagg 61 gaacaaaagc tggagctctc tagaggccat ggcggccgca ctaggctgca gtgcagcgtg 121 acccggtcgt gcccctctct agagataatg agcattgcat gtctaagtta taaaaaatta 181 ccacatattt tttttgtcac acttgtttga agtgcagttt atctatcttt atacatatat 241 ttaaacttta ctctacgaat aatataatct atagtactac aataatatca gtgttttaga 301 gaatcatata aatgaacagt tagacatggt ctaaaggaca attgagtatt ttgacaacag 361 gactctacag ttttatcttt ttagtgtgca tgtgttctcc tttttttttg caaatagctt 421 cacctatata atacttcatc cattttatta gtacatccat ttagggttta gggttaatgg 481 tttttataga ctaatttttt tagtacatct attttattct attttagcct ctaaattaag 541 aaaactaaaa ctctatttta gtttttttat ttaataattt agatataaaa tagaataaaa 601 taaagtgact aaaaattaaa caaataccct ttaagaaatt aaaaaaacta aggaaacatt 661 tttcttgttt cgagtagata atgccagcct gttaaacgcc gtcgatcgac gagtctaacg 721 gacaccaacc agcgaaccag cagcgtcgcg tcgggccaag cgaagcagac ggcacggcat 781 ctctgtcgct gcctctggac ccctctcgag agttccgctc caccgttgga cttgctccgc 841 tgtcggcatc cagaaattgc gtggcggagc ggcagacgtg agccggcacg gcaggcggcc 901 tcctcctcct ctcacggcac cggcagctac gggggattcc tttcccaccg ctccttcgct 961 ttcccttcct cgcccgccgt aataaataga caccccctcc acaccctctt tccccaacct 1021 cgtgttgttc ggagcgcaca cacacacaac cagatctccc ccaaatccac ccgtcggcac 1081 ctccgcttca aggtacgccg ctcgtcctcc cccccccccc ctctctacct tctctagatc 1141 ggcgttccgg tccatggtta gggcccggta gttctacttc tgttcatgtt tgtgttagat 1201 ccgtgtttgt gttagatccg tgctgctagc gttcgtacac ggatgcgacc tgtacgtcag 1261 acacgttctg attgctaact tgccagtgtt tctctttggg gaatcctggg atggctctag 1321 ccgttccgca gacgggatcg atctaggata ggtatacatg ttgatgtggg ttttactgat 1381 gcatatacat gatggcatat gcagcatcta ttcatatgct ctaaccttga gtacctatct 1441 attataataa acaagtatgt tttataatta ttttgatctt gatatacttg gatgatggca 1501 tatgcagcag ctatatgtgg atttttttag ccctgccttc atacgctatt tatttgcttg 1561 gtactgtttc ttttgtcgat gctcaccctg ttgtttggtg ttacttctgc aggtactagt 1621 tggatccccc gggctgcagg aattcaagct tacgcgttgt acaggagtac cgccccgtcc 1681 ggtcctgccc gtcaccgaga tcagcggaga gttcatggtc tccaagggcg aggagcttat 1741 caaggagaac atgcacatga agctctacat ggaaggaacc gtgaacaatc accacttcaa 1801 atgcaccagc gagggtgagg gcaagcccta cgaggggact cagaccatgc gcatcaaggt 1861 tgtcgaggga ggcccattgc ctttcgcctt cgacattctg gctacgtctt tcatgtacgg 1921 ctcacgtacc ttcatcaacc atacccaagg aatcccggac ttctttaagc agtccttccc 1981 cgagggcttc acctgggaaa gggtgacaac ctacgaggat ggtggagtcc tcactgccac 2041 ccaggacacc agcctccagg acggctgctt gatctacaac gtgaagattc gcggggttaa 2101 cttcccatcg aacggccccg tcatgcaaaa gaagaccctg ggatgggagg caaacacgga 2161 gatgctctat cctgccgacg gcggacttga gggcagaacc gacatggcgt tgaagctcgt 2221 gggtggcggg cacctgatct gcaatttcaa gactacctac cgctccaaga agccagccaa 2281 gaacctcaag atgccgggag tctactacgt ggatcaccgt cttgagagga tcaaggaagc 2341 cgacaaggag acctacgttg agcagcacga ggtcgctgtg gcccgctact gcgacttgcc 2401 cagcaagctg ggccacaagc tcaactgagt cgaccctgct ttaatgagat atgcgagacg 2461 cctatgatcg catgatattt gctttcaatt ctgttgtgca cgttgtaaaa aacctgagca 2521 tgtgtagctc agatccttac cgccggtttc ggttcattct aatgaatata tcacccgtta 2581 ctatcgtatt tttatgaata atattctccg ttcaatttac tgattgtcct cgacaggcct 2641 taagggccag atcttgggcc cggtacccaa ttcgccctat agtgagtcgt attacaattc 2701 actggccgtc gttttacaac gtcgtgactg ggaaaaccct ggcgttaccc aacttaatcg 2761 ccttgcagca catccccctt tcgccagctg gcgtaatagc gaagaggccc gcaccgatcg 2821 cccttcccaa cagttgcgca gcctgaatgg cgaatggaaa ttgtaagcgt taatattttg 2881 ttaaaattcg cgttaaattt ttgttaaatc agctcatttt ttaaccaata ggccgaaatc 2941 ggcaaaatcc cttataaatc aaaagaatag accgagatag ggttgagtgt tgttccagtt 3001 tggaacaaga gtccactatt aaagaacgtg gactccaacg tcaaagggcg aaaaaccgtc 3061 tatcagggcg atggcccact acgtgaacca tcaccctaat caagtttttt ggggtcgagg 3121 tgccgtaaag cactaaatcg gaaccctaaa gggagccccc gatttagagc ttgacgggga 3181 aagccggcga acgtggcgag aaaggaaggg aagaaagcga aaggagcggg cgctagggcg 3241 ctggcaagtg tagcggtcac gctgcgcgta accaccacac ccgccgcgct taatgcgccg 3301 ctacagggcg cgtcaggtgg cacttttcgg ggaaatgtgc gcggaacccc tatttgttta 3361 tttttctaaa tacattcaaa tatgtatccg ctcatgagac aataaccctg ataaatgctt 3421 caataatatt gaaaaaggaa gagtatgagt attcaacatt tccgtgtcgc ccttattccc 3481 ttttttgcgg cattttgcct tcctgttttt gctcacccag aaacgctggt gaaagtaaaa 3541 gatgctgaag atcagttggg tgcacgagtg ggttacatcg aactggatct caacagcggt 3601 aagatccttg agagttttcg ccccgaagaa cgttttccaa tgatgagcac ttttaaagtt 3661 ctgctatgtg gcgcggtatt atcccgtatt gacgccgggc aagagcaact cggtcgccgc 3721 atacactatt ctcagaatga cttggttgag tactcaccag tcacagaaaa gcatcttacg 3781 gatggcatga cagtaagaga attatgcagt gctgccataa ccatgagtga taacactgcg 3841 gccaacttac ttctgacaac gatcggagga ccgaaggagc taaccgcttt tttgcacaac 3901 atgggggatc atgtaactcg ccttgatcgt tgggaaccgg agctgaatga agccatacca 3961 aacgacgagc gtgacaccac gatgcctgta gcaatggcaa caacgttgcg caaactatta 4021 actggcgaac tacttactct agcttcccgg caacaattaa tagactggat ggaggcggat 4081 aaagttgcag gaccacttct gcgctcggcc cttccggctg gctggtttat tgctgataaa 4141 tctggagccg gtgagcgtgg gtctcgcggt atcattgcag cactggggcc agatggtaag 4201 ccctcccgta tcgtagttat ctacacgacg gggagtcagg caactatgga tgaacgaaat 4261 agacagatcg ctgagatagg tgcctcactg attaagcatt ggtaactgtc agaccaagtt 4321 tactcatata tactttagat tgatttaaaa cttcattttt aatttaaaag gatctaggtg 4381 aagatccttt ttgataatct catgaccaaa atcccttaac gtgagttttc gttccactga 4441 gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag atcctttttt tctgcgcgta 4501 atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa 4561 gagctaccaa ctctttttcc gaaggtaact ggcttcagca gagcgcagat accaaatact 4621 gtccttctag tgtagccgta gttaggccac cacttcaaga actctgtagc accgcctaca 4681 tacctcgctc tgctaatcct gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt 4741 accgggttgg actcaagacg atagttaccg gataaggcgc agcggtcggg ctgaacgggg 4801 ggttcgtgca cacagcccag cttggagcga acgacctaca ccgaactgag atacctacag 4861 cgtgagctat gagaaagcgc cacgcttccc gaagggagaa aggcggacag gtatccggta 4921 agcggcaggg tcggaacagg agagcgcacg agggagcttc cagggggaaa cgcctggtat 4981 ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt gtgatgctcg 5041 tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc 5101 ttttgctggc cttttgctca catgttcttt cctgcgttat cccctgattc tgtggataac 5161 cgtattaccg cctttgagtg agctgatacc gctcgccgca gccgaacgac cgagcgcagc 5221 gagtcagtga gcgaggaagc ggaagagcgc ccaatacgca aaccgcctct ccccgcgcgt 5281 tggccgattc attaatgcag ctggcacgac aggtttcccg actggaaagc gggcagtgag 5341 cgcaacgcaa ttaatgtgag ttagctcact cattaggcac cccaggcttt acactttatg 5401 cttccggctc gtatgttgtg tggaattgtg agcggataac aattt //