LOCUS       B648pUbiZm-LRFPi        5634 bp    DNA     circular SYN 08-FEB-2016
DEFINITION  Ligation of RFPi* into B633pUbiZm-LGFP*
ACCESSION   B648pUbiZm-LRFPi
KEYWORDS    .
SOURCE      Unknown.
  ORGANISM  Unknown
            Unclassified.
REFERENCE   1  (bases 1 to 5634)
  AUTHORS   Self
  JOURNAL   Unpublished.
COMMENT     SECID/File created by Clone Manager, Scientific & Educational Software
COMMENT     SECNOTES|Vector molecule:  B633pUbiZm-LGFP*
               Fragment ends: SalI and MluI
               Fragment size: 4668
            Insert molecule:  RFPi*
               Fragment ends: MluI and SalI
               Fragment size: 966
            Genesynthesis
            Sequencing primers	
            Ubi1	CTGTTTCTTTTGTCGATGC
FEATURES             Location/Qualifiers
     misc_feature    106..1614
                     /gene="UbiZm"
                     /product="Promotor"
                     /SECDrawAs="Region"
                     /SECStyleId=1
     misc_feature    1093..1612
                     /gene="int"
                     /product="Intron"
                     /SECDrawAs="Region"
                     /SECStyleId=1
     misc_feature    1664..1714
                     /gene="link"
                     /SECDrawAs="Region"
                     /SECStyleId=1
     CDS             1715..1860
                     /gene="TagRFP'"
                     /translation="MVSKGEELIKENMHMKLYMEGTVNNHHFKCTSEGEGKPYEGTQT
                     MRIK"
                     /SECDrawAs="Gene"
                     /SECStyleId=1
                     /SECName="tagrfp"
     CDS             1861..2047
                     /gene="int"
                     /product="STLS1"
                     /SECDrawAs="Gene"
                     /SECStyleId=1
     CDS             2048..2617
                     /gene="'TagRFP"
                     /codon_start=2
                     /translation="LSREAHCLSPSTFWLRLSCTAHVPSSTIPKESRTSLSSPSPRAS
                     PGKG"
                     /SECDrawAs="Gene"
                     /SECStyleId=1
                     /SECName="tagrfp"
     misc_feature    2623..2818
                     /gene="Tocs"
                     /product="Terminator"
                     /SECDrawAs="Region"
                     /SECStyleId=1
                     /SECName="Tocs"
                     /SECDescr="Terminator"
     misc_feature    3634..4494
                     /gene="Amp"
                     /product="ORF-1"
                     /SECDrawAs="Gene"
                     /SECStyleId=1
                     /SECName="Amp"
                     /SECDescr="ORF-1"
     CDS             4537..5253
                     /gene="ColE1"
                     /product="ColE1"
                     /SECDrawAs="Region"
                     /SECStyleId=1
                     /SECName="ColE1"
                     /SECDescr="ColE1"
ORIGIN      
        1 cacacaggaa acagctatga ccatgattac gccaagctcg aaattaaccc tcactaaagg
       61 gaacaaaagc tggagctctc tagaggccat ggcggccgca ctaggctgca gtgcagcgtg
      121 acccggtcgt gcccctctct agagataatg agcattgcat gtctaagtta taaaaaatta
      181 ccacatattt tttttgtcac acttgtttga agtgcagttt atctatcttt atacatatat
      241 ttaaacttta ctctacgaat aatataatct atagtactac aataatatca gtgttttaga
      301 gaatcatata aatgaacagt tagacatggt ctaaaggaca attgagtatt ttgacaacag
      361 gactctacag ttttatcttt ttagtgtgca tgtgttctcc tttttttttg caaatagctt
      421 cacctatata atacttcatc cattttatta gtacatccat ttagggttta gggttaatgg
      481 tttttataga ctaatttttt tagtacatct attttattct attttagcct ctaaattaag
      541 aaaactaaaa ctctatttta gtttttttat ttaataattt agatataaaa tagaataaaa
      601 taaagtgact aaaaattaaa caaataccct ttaagaaatt aaaaaaacta aggaaacatt
      661 tttcttgttt cgagtagata atgccagcct gttaaacgcc gtcgatcgac gagtctaacg
      721 gacaccaacc agcgaaccag cagcgtcgcg tcgggccaag cgaagcagac ggcacggcat
      781 ctctgtcgct gcctctggac ccctctcgag agttccgctc caccgttgga cttgctccgc
      841 tgtcggcatc cagaaattgc gtggcggagc ggcagacgtg agccggcacg gcaggcggcc
      901 tcctcctcct ctcacggcac cggcagctac gggggattcc tttcccaccg ctccttcgct
      961 ttcccttcct cgcccgccgt aataaataga caccccctcc acaccctctt tccccaacct
     1021 cgtgttgttc ggagcgcaca cacacacaac cagatctccc ccaaatccac ccgtcggcac
     1081 ctccgcttca aggtacgccg ctcgtcctcc cccccccccc ctctctacct tctctagatc
     1141 ggcgttccgg tccatggtta gggcccggta gttctacttc tgttcatgtt tgtgttagat
     1201 ccgtgtttgt gttagatccg tgctgctagc gttcgtacac ggatgcgacc tgtacgtcag
     1261 acacgttctg attgctaact tgccagtgtt tctctttggg gaatcctggg atggctctag
     1321 ccgttccgca gacgggatcg atctaggata ggtatacatg ttgatgtggg ttttactgat
     1381 gcatatacat gatggcatat gcagcatcta ttcatatgct ctaaccttga gtacctatct
     1441 attataataa acaagtatgt tttataatta ttttgatctt gatatacttg gatgatggca
     1501 tatgcagcag ctatatgtgg atttttttag ccctgccttc atacgctatt tatttgcttg
     1561 gtactgtttc ttttgtcgat gctcaccctg ttgtttggtg ttacttctgc aggtactagt
     1621 tggatccccc gggctgcagg aattcaagct tacgcgttgt acaggagtac cgccccgtcc
     1681 ggtcctgccc gtcaccgaga tcagcggaga gttcatggtc tccaagggcg aggagcttat
     1741 caaggagaac atgcacatga agctctacat ggaaggaacc gtgaacaatc accacttcaa
     1801 atgcaccagc gagggtgagg gcaagcccta cgaggggact cagaccatgc gcatcaaggt
     1861 aagtttctgc ttctaccttt gatatatata taataattat cattaattag tagtaatata
     1921 atatttcaaa tatttttttc aaaataaaag aatgtagtat atagcaattg cttttctgta
     1981 gtttataagt gtgtatattt taatttataa cttttctaat atatgaccaa aatttgttga
     2041 tgtgcaggtt gtcgagggag gcccattgcc tttcgccttc gacattctgg ctacgtcttt
     2101 catgtacggc tcacgtacct tcatcaacca tacccaagga atcccggact tctttaagca
     2161 gtccttcccc gagggcttca cctgggaaag ggtgacaacc tacgaggatg gtggagtcct
     2221 cactgccacc caggacacca gcctccagga cggctgcttg atctacaacg tgaagattcg
     2281 cggggttaac ttcccatcga acggccccgt catgcaaaag aagaccctgg gatgggaggc
     2341 aaacacggag atgctctatc ctgccgacgg cggacttgag ggcagaaccg acatggcgtt
     2401 gaagctcgtg ggtggcgggc acctgatctg caatttcaag actacctacc gctccaagaa
     2461 gccagccaag aacctcaaga tgccgggagt ctactacgtg gatcaccgtc ttgagaggat
     2521 caaggaagcc gacaaggaga cctacgttga gcagcacgag gtcgctgtgg cccgctactg
     2581 cgacttgccc agcaagctgg gccacaagct caactgagtc gaccctgctt taatgagata
     2641 tgcgagacgc ctatgatcgc atgatatttg ctttcaattc tgttgtgcac gttgtaaaaa
     2701 acctgagcat gtgtagctca gatccttacc gccggtttcg gttcattcta atgaatatat
     2761 cacccgttac tatcgtattt ttatgaataa tattctccgt tcaatttact gattgtcctc
     2821 gacaggcctt aagggccaga tcttgggccc ggtacccaat tcgccctata gtgagtcgta
     2881 ttacaattca ctggccgtcg ttttacaacg tcgtgactgg gaaaaccctg gcgttaccca
     2941 acttaatcgc cttgcagcac atcccccttt cgccagctgg cgtaatagcg aagaggcccg
     3001 caccgatcgc ccttcccaac agttgcgcag cctgaatggc gaatggaaat tgtaagcgtt
     3061 aatattttgt taaaattcgc gttaaatttt tgttaaatca gctcattttt taaccaatag
     3121 gccgaaatcg gcaaaatccc ttataaatca aaagaataga ccgagatagg gttgagtgtt
     3181 gttccagttt ggaacaagag tccactatta aagaacgtgg actccaacgt caaagggcga
     3241 aaaaccgtct atcagggcga tggcccacta cgtgaaccat caccctaatc aagttttttg
     3301 gggtcgaggt gccgtaaagc actaaatcgg aaccctaaag ggagcccccg atttagagct
     3361 tgacggggaa agccggcgaa cgtggcgaga aaggaaggga agaaagcgaa aggagcgggc
     3421 gctagggcgc tggcaagtgt agcggtcacg ctgcgcgtaa ccaccacacc cgccgcgctt
     3481 aatgcgccgc tacagggcgc gtcaggtggc acttttcggg gaaatgtgcg cggaacccct
     3541 atttgtttat ttttctaaat acattcaaat atgtatccgc tcatgagaca ataaccctga
     3601 taaatgcttc aataatattg aaaaaggaag agtatgagta ttcaacattt ccgtgtcgcc
     3661 cttattccct tttttgcggc attttgcctt cctgtttttg ctcacccaga aacgctggtg
     3721 aaagtaaaag atgctgaaga tcagttgggt gcacgagtgg gttacatcga actggatctc
     3781 aacagcggta agatccttga gagttttcgc cccgaagaac gttttccaat gatgagcact
     3841 tttaaagttc tgctatgtgg cgcggtatta tcccgtattg acgccgggca agagcaactc
     3901 ggtcgccgca tacactattc tcagaatgac ttggttgagt actcaccagt cacagaaaag
     3961 catcttacgg atggcatgac agtaagagaa ttatgcagtg ctgccataac catgagtgat
     4021 aacactgcgg ccaacttact tctgacaacg atcggaggac cgaaggagct aaccgctttt
     4081 ttgcacaaca tgggggatca tgtaactcgc cttgatcgtt gggaaccgga gctgaatgaa
     4141 gccataccaa acgacgagcg tgacaccacg atgcctgtag caatggcaac aacgttgcgc
     4201 aaactattaa ctggcgaact acttactcta gcttcccggc aacaattaat agactggatg
     4261 gaggcggata aagttgcagg accacttctg cgctcggccc ttccggctgg ctggtttatt
     4321 gctgataaat ctggagccgg tgagcgtggg tctcgcggta tcattgcagc actggggcca
     4381 gatggtaagc cctcccgtat cgtagttatc tacacgacgg ggagtcaggc aactatggat
     4441 gaacgaaata gacagatcgc tgagataggt gcctcactga ttaagcattg gtaactgtca
     4501 gaccaagttt actcatatat actttagatt gatttaaaac ttcattttta atttaaaagg
     4561 atctaggtga agatcctttt tgataatctc atgaccaaaa tcccttaacg tgagttttcg
     4621 ttccactgag cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga tccttttttt
     4681 ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg
     4741 ccggatcaag agctaccaac tctttttccg aaggtaactg gcttcagcag agcgcagata
     4801 ccaaatactg tccttctagt gtagccgtag ttaggccacc acttcaagaa ctctgtagca
     4861 ccgcctacat acctcgctct gctaatcctg ttaccagtgg ctgctgccag tggcgataag
     4921 tcgtgtctta ccgggttgga ctcaagacga tagttaccgg ataaggcgca gcggtcgggc
     4981 tgaacggggg gttcgtgcac acagcccagc ttggagcgaa cgacctacac cgaactgaga
     5041 tacctacagc gtgagctatg agaaagcgcc acgcttcccg aagggagaaa ggcggacagg
     5101 tatccggtaa gcggcagggt cggaacagga gagcgcacga gggagcttcc agggggaaac
     5161 gcctggtatc tttatagtcc tgtcgggttt cgccacctct gacttgagcg tcgatttttg
     5221 tgatgctcgt caggggggcg gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg
     5281 ttcctggcct tttgctggcc ttttgctcac atgttctttc ctgcgttatc ccctgattct
     5341 gtggataacc gtattaccgc ctttgagtga gctgataccg ctcgccgcag ccgaacgacc
     5401 gagcgcagcg agtcagtgag cgaggaagcg gaagagcgcc caatacgcaa accgcctctc
     5461 cccgcgcgtt ggccgattca ttaatgcagc tggcacgaca ggtttcccga ctggaaagcg
     5521 ggcagtgagc gcaacgcaat taatgtgagt tagctcactc attaggcacc ccaggcttta
     5581 cactttatgc ttccggctcg tatgttgtgt ggaattgtga gcggataaca attt
//
