LOCUS       B727p35s-YFPiD          4492 bp    DNA     circular SYN 26-JAN-2016
DEFINITION  Ligation of YFPi-Mlu-Sal* into B589p35s-mGFPD-ocs*
ACCESSION   B727p35s-YFPiD
KEYWORDS    .
SOURCE      Unknown.
  ORGANISM  Unknown
            Unclassified.
REFERENCE   1  (bases 1 to 4492)
  AUTHORS   Self
  JOURNAL   Unpublished.
COMMENT     SECID/File created by Clone Manager, Scientific & Educational Software
COMMENT     SECNOTES|Vector molecule:  B589p35s-mGFPD-ocs*
               Fragment ends: SalI and MluI
               Fragment size: 3563
            Insert molecule:  YFPi-Mlu-Sal*
               Fragment ends: MluI and SalI
               Fragment size: 929
            Primer for gene synthesis
            35F	CACTATCCTTCGCAAGACCCTTCCTCTA
            YFPR1	caaaggtagaagcagaaacttaCCTTTCAGCTCGATG
            STLS	GGTAAGTTTCTGCTTCTACCT
            Sir	CCTGCACATCAACAAATTTTG
            YFPF3	gaccaaaatttgttgatgtgcaGGTATCGATTTCAAGGAAG
            OcsR	GCGATCATAGGCGTCTCGCATATCTC
            Sequencing primers:
            35S	GCACAATCCCACTATCCTT
FEATURES             Location/Qualifiers
     misc_feature    144..562
                     /gene="P35S"
                     /product="Promotor-Region"
                     /SECDrawAs="Region"
                     /SECStyleId=1
                     /SECName="P35S"
                     /SECDescr="Promotor-Region"
     CDS             590..971
                     /gene="YFP'"
                     /product="mCtrine (codon optimized for plants)"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLT
                     LKFICTTGKLPVPWPTLVTTFGYGLMCFARYPDHMKQHDFFKSAMPEGYVQERTIFFK
                     DDGNYKTRAEVKFEGDTLVNRIELK"
                     /SECDrawAs="Gene"
                     /SECStyleId=1
                     /SECName="yfp"
                     /SECDescr="mCitrine (codon optimized for plants)"
     CDS             972..1160
                     /gene="int"
                     /product="STLS1"
                     /SECDrawAs="Gene"
                     /SECStyleId=1
     CDS             1161..1498
                     /gene="'YFP"
                     /product="mCtrine (codon optimized for plants)"
                     /codon_start=2
                     /translation="YRFQGRWKYSRTQVGVQLQQP"
                     /SECDrawAs="Gene"
                     /SECStyleId=1
                     /SECName="yfp"
                     /SECDescr="mCitrine (codon optimized for plants)"
     misc_feature    1507..1702
                     /gene="Tocs"
                     /product="Terminator"
                     /SECDrawAs="Region"
                     /SECStyleId=1
                     /SECName="Tocs"
                     /SECDescr="Terminator"
     misc_feature    2518..3378
                     /gene="Amp"
                     /product="ORF-1"
                     /SECDrawAs="Gene"
                     /SECStyleId=1
                     /SECName="Amp"
                     /SECDescr="ORF-1"
     CDS             3421..4137
                     /gene="ColE1"
                     /product="ColE1"
                     /SECDrawAs="Region"
                     /SECStyleId=1
                     /SECName="ColE1"
                     /SECDescr="ColE1"
ORIGIN      
        1 gtggaattgt gagcggataa caatttcaca caggaaacag ctatgaccat gattacgcca
       61 agctcgaaat taaccctcac taaagggaac aaaagctgga gctctctaga ggccatggcg
      121 gccgcactag tggatccccc gggtcaacat ggtggagcac gacactctcg tctactccaa
      181 gaatatcaaa gatacagtct cagaagacca gagggctatt gagacttttc aacaaagggt
      241 aatatcggga aacctcctcg gattccattg cccagctatc tgtcacttca tcgaaaggac
      301 agtagaaaag gaagatggct tctacaaatg ccatcattgc gataaaggaa aggctatcgt
      361 tcaagatgcc tctaccgaca gtggtcccaa agatggaccc ccacccacga ggaacatcgt
      421 ggaaaaagaa gacgttccaa ccacgtcttc aaagcaagtg gattgatgtg atatctccac
      481 tgacgtaagg gatgacgcac aatcccacta tccttcgcaa gacccttcct ctatataagg
      541 aagttcattt catttggaga ggacacaagc ttacgcgtcc tccataacta tggtgtccaa
      601 gggcgaggag ctgttcaccg gagtcgttcc catcctcgtc gaattggacg gcgacgtgaa
      661 cggtcacaag ttcagcgtgt ctggggaggg cgagggagat gccacctacg gcaagctcac
      721 tctcaagttc atttgcacca ccggaaagct gccagtccct tggccgaccc tcgttactac
      781 cttcggctac ggtttgatgt gcttcgctcg ctaccccgac cacatgaagc agcacgactt
      841 ctttaagtcc gccatgccag agggctacgt ccaagagagg accatcttct tcaaggacga
      901 tgggaactac aagacccgcg ccgaagtgaa gttcgagggc gacaccctgg tgaaccgcat
      961 cgagctgaaa ggtaagtttc tgcttctacc tttgatatat atataataat tatcattaat
     1021 tagtagtaat ataatatttc aaatattttt ttcaaaataa aagaatgtag tatatagcaa
     1081 ttgcttttct gtagtttata agtgtgtata ttttaattta taacttttct aatatatgac
     1141 caaaatttgt tgatgtgcag gtatcgattt caaggaagat ggaaatattc tcggacacaa
     1201 gttggagtac aactacaaca gccataacgt gtatatcatg gctgacaagc agaagaacgg
     1261 catcaaagtc aacttcaaga tcaggcacaa tattgaggat ggttcggtgc agctcgccga
     1321 ccactaccag caaaacactc ctatcgggga cggcccggtt cttctccccg acaaccacta
     1381 cctgtcctac cagagcgccc tctccaagga cccaaacgaa aagcgcgatc acatggtctt
     1441 gcttgagttc gtgaccgctg ccggaatcac cctgggcatg gacgagcttt acaagtgact
     1501 cgtcgaccct gctttaatga gatatgcgag acgcctatga tcgcatgata tttgctttca
     1561 attctgttgt gcacgttgta aaaaacctga gcatgtgtag ctcagatcct taccgccggt
     1621 ttcggttcat tctaatgaat atatcacccg ttactatcgt atttttatga ataatattct
     1681 ccgttcaatt tactgattgt cctcgacagg ccttaagggc cagatcttgg gcccggtacc
     1741 caattcgccc tatagtgagt cgtattacaa ttcactggcc gtcgttttac aacgtcgtga
     1801 ctgggaaaac cctggcgtta cccaacttaa tcgccttgca gcacatcccc ctttcgccag
     1861 ctggcgtaat agcgaagagg cccgcaccga tcgcccttcc caacagttgc gcagcctgaa
     1921 tggcgaatgg aaattgtaag cgttaatatt ttgttaaaat tcgcgttaaa tttttgttaa
     1981 atcagctcat tttttaacca ataggccgaa atcggcaaaa tcccttataa atcaaaagaa
     2041 tagaccgaga tagggttgag tgttgttcca gtttggaaca agagtccact attaaagaac
     2101 gtggactcca acgtcaaagg gcgaaaaacc gtctatcagg gcgatggccc actacgtgaa
     2161 ccatcaccct aatcaagttt tttggggtcg aggtgccgta aagcactaaa tcggaaccct
     2221 aaagggagcc cccgatttag agcttgacgg ggaaagccgg cgaacgtggc gagaaaggaa
     2281 gggaagaaag cgaaaggagc gggcgctagg gcgctggcaa gtgtagcggt cacgctgcgc
     2341 gtaaccacca cacccgccgc gcttaatgcg ccgctacagg gcgcgtcagg tggcactttt
     2401 cggggaaatg tgcgcggaac ccctatttgt ttatttttct aaatacattc aaatatgtat
     2461 ccgctcatga gacaataacc ctgataaatg cttcaataat attgaaaaag gaagagtatg
     2521 agtattcaac atttccgtgt cgcccttatt cccttttttg cggcattttg ccttcctgtt
     2581 tttgctcacc cagaaacgct ggtgaaagta aaagatgctg aagatcagtt gggtgcacga
     2641 gtgggttaca tcgaactgga tctcaacagc ggtaagatcc ttgagagttt tcgccccgaa
     2701 gaacgttttc caatgatgag cacttttaaa gttctgctat gtggcgcggt attatcccgt
     2761 attgacgccg ggcaagagca actcggtcgc cgcatacact attctcagaa tgacttggtt
     2821 gagtactcac cagtcacaga aaagcatctt acggatggca tgacagtaag agaattatgc
     2881 agtgctgcca taaccatgag tgataacact gcggccaact tacttctgac aacgatcgga
     2941 ggaccgaagg agctaaccgc ttttttgcac aacatggggg atcatgtaac tcgccttgat
     3001 cgttgggaac cggagctgaa tgaagccata ccaaacgacg agcgtgacac cacgatgcct
     3061 gtagcaatgg caacaacgtt gcgcaaacta ttaactggcg aactacttac tctagcttcc
     3121 cggcaacaat taatagactg gatggaggcg gataaagttg caggaccact tctgcgctcg
     3181 gcccttccgg ctggctggtt tattgctgat aaatctggag ccggtgagcg tgggtctcgc
     3241 ggtatcattg cagcactggg gccagatggt aagccctccc gtatcgtagt tatctacacg
     3301 acggggagtc aggcaactat ggatgaacga aatagacaga tcgctgagat aggtgcctca
     3361 ctgattaagc attggtaact gtcagaccaa gtttactcat atatacttta gattgattta
     3421 aaacttcatt tttaatttaa aaggatctag gtgaagatcc tttttgataa tctcatgacc
     3481 aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag accccgtaga aaagatcaaa
     3541 ggatcttctt gagatccttt ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca
     3601 ccgctaccag cggtggtttg tttgccggat caagagctac caactctttt tccgaaggta
     3661 actggcttca gcagagcgca gataccaaat actgtccttc tagtgtagcc gtagttaggc
     3721 caccacttca agaactctgt agcaccgcct acatacctcg ctctgctaat cctgttacca
     3781 gtggctgctg ccagtggcga taagtcgtgt cttaccgggt tggactcaag acgatagtta
     3841 ccggataagg cgcagcggtc gggctgaacg gggggttcgt gcacacagcc cagcttggag
     3901 cgaacgacct acaccgaact gagataccta cagcgtgagc tatgagaaag cgccacgctt
     3961 cccgaaggga gaaaggcgga caggtatccg gtaagcggca gggtcggaac aggagagcgc
     4021 acgagggagc ttccaggggg aaacgcctgg tatctttata gtcctgtcgg gtttcgccac
     4081 ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac
     4141 gccagcaacg cggccttttt acggttcctg gccttttgct ggccttttgc tcacatgttc
     4201 tttcctgcgt tatcccctga ttctgtggat aaccgtatta ccgcctttga gtgagctgat
     4261 accgctcgcc gcagccgaac gaccgagcgc agcgagtcag tgagcgagga agcggaagag
     4321 cgcccaatac gcaaaccgcc tctccccgcg cgttggccga ttcattaatg cagctggcac
     4381 gacaggtttc ccgactggaa agcgggcagt gagcgcaacg caattaatgt gagttagctc
     4441 actcattagg caccccaggc tttacacttt atgcttccgg ctcgtatgtt gt
//
