LOCUS       D894p35s-LmEGFPo        4364 bp    DNA     circular SYN 26-AUG-2020
DEFINITION  35s-LmEGFPoi Circularized
ACCESSION   D894p35s-LmEGFPo
KEYWORDS    .
SOURCE      Unknown.
  ORGANISM  Unknown
            Unclassified.
REFERENCE   1  (bases 1 to 4364)
  AUTHORS   Self
  JOURNAL   Unpublished.
COMMENT     SECID/File created by Clone Manager, Scientific & Educational Software
COMMENT     SECNOTES|Primers for gene synthesis
            GFPoiF	GTATCGATTTCAAGGAAGATGGA
            GFPoiR	CTTTCAGCTCGATGCG
            Sequencing primers:
            M13RY	CACACAGGAAACAGCTATGAC
            GFP-F	GACTATCTTCTTCAAGGATGAC
FEATURES             Location/Qualifiers
     misc_feature    106..528
                     /gene="35S-Pro"
                     /product="Promotor-Region"
                     /SECDrawAs="Region"
                     /SECStyleId=1
                     /SECName="P35S"
                     /SECDescr="Promotor-Region"
     CDS             494..500
                     /gene="TATA"
                     /product="TATA-box"
                     /translation="YI"
                     /SECDrawAs="Region"
                     /SECStyleId=1
                     /SECDescr="TATA-box"
     CDS             627..1343
                     /gene="ORF-3"
                     /translation="MGKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTFTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKD
                     DGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNG
                     IKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHM
                     VLLEFVTAAGITHGMDELYK"
                     /SECDrawAs="Gene"
                     /SECStyleId=1
                     /SECName="mEGFP"
     misc_feature    816..821
                     /product="F64L/S65T - enhanced fluorescence"
                     /SECDrawAs="Region"
                     /SECStyleId=1
     misc_feature    1242..1244
                     /product="A206K - monomeric protein"
                     /SECDrawAs="Region"
                     /SECStyleId=1
     misc_feature    1353..1548
                     /gene="OCS"
                     /product="Terminator"
                     /SECDrawAs="Region"
                     /SECStyleId=1
                     /SECName="Tocs"
                     /SECDescr="Terminator"
     misc_feature    2364..3224
                     /gene="Amp"
                     /product="ORF-1"
                     /SECDrawAs="Region"
                     /SECStyleId=1
                     /SECName="Amp"
                     /SECDescr="ORF-1"
     misc_feature    3267..3983
                     /gene="ColE1"
                     /product="ColE1"
                     /SECDrawAs="Region"
                     /SECStyleId=1
                     /SECName="ColE1"
                     /SECDescr="ColE1"
ORIGIN      
        1 cacacaggaa acagctatga ccatgattac gccaagctcg aaattaaccc tcactaaagg
       61 gaacaaaagc tggagctctc tagaggccat ggcggccgca ctagatcaac atggtggagc
      121 acgacactct cgtctactcc aagaatatca aagatacagt ctcagaagac cagagggcta
      181 ttgagacttt tcaacaaagg gtaatatcgg gaaacctcct cggattccat tgcccagcta
      241 tctgtcactt catcgaaagg acagtagaaa aggaagatgg cttctacaaa tgccatcatt
      301 gcgataaagg aaaggctatc gttcaagatg cctctaccga cagtggtccc aaagatggac
      361 ccccacccac gaggaacatc gtggaaaaag aagacgttcc aaccacgtct tcaaagcaag
      421 tggattgatg tgatatctcc actgacgtaa gggatgacgc acaatcccac tatccttcgc
      481 aagacccttc ctctatataa ggaagttcat ttcatttgga gaggacacta gttggatccc
      541 ccgggctgca ggaattcaag cttacgcgtt gtacaggagt accgccccgt ccggtcctgc
      601 ccgtcaccga gatcagcgga gagttcatgg gcaagggcga ggaactgttc actggcgtgg
      661 tcccaatcct ggtggaactg gatggtgatg tgaacgggca caagttctcc gtcagcggag
      721 agggtgaagg tgatgccacc tacggaaagc tcaccctgaa gttcatctgc actaccggaa
      781 agctccctgt tccgtggcca accctcgtca ccactttcac ctacggtgtt cagtgcttct
      841 cccgttaccc agatcacatg aagcagcatg acttcttcaa gagcgccatg cccgaaggct
      901 acgtgcaaga aaggactatc ttcttcaagg atgacgggaa ctacaagaca cgtgccgaag
      961 tcaagttcga aggtgatacc ctggtgaacc gcatcgagct gaaaggtatc gatttcaagg
     1021 aagatggaaa catcctcgga cacaagctgg agtacaacta caactcccac aacgtataca
     1081 tcatggccga caagcagaag aacggcatca aggtgaactt caagatcagg cacaacatcg
     1141 aagatggaag cgtgcaactg gcggaccact accagcagaa cacgcccatc ggcgatggcc
     1201 ctgtcctgct gccggacaac cattacctgt ccacgcaatc taagctctcc aaggacccca
     1261 acgagaagag ggaccacatg gtcctgctgg agttcgtgac ggctgctggg atcacgcatg
     1321 gcatggatga actctacaag tgacccggtc gaccctgctt taatgagata tgcgagacgc
     1381 ctatgatcgc atgatatttg ctttcaattc tgttgtgcac gttgtaaaaa acctgagcat
     1441 gtgtagctca gatccttacc gccggtttcg gttcattcta atgaatatat cacccgttac
     1501 tatcgtattt ttatgaataa tattctccgt tcaatttact gattgtcctc gacaggcctt
     1561 aagggccaga tcttgggccc ggtacccaat tcgccctata gtgagtcgta ttacaattca
     1621 ctggccgtcg ttttacaacg tcgtgactgg gaaaaccctg gcgttaccca acttaatcgc
     1681 cttgcagcac atcccccttt cgccagctgg cgtaatagcg aagaggcccg caccgatcgc
     1741 ccttcccaac agttgcgcag cctgaatggc gaatggaaat tgtaagcgtt aatattttgt
     1801 taaaattcgc gttaaatttt tgttaaatca gctcattttt taaccaatag gccgaaatcg
     1861 gcaaaatccc ttataaatca aaagaataga ccgagatagg gttgagtgtt gttccagttt
     1921 ggaacaagag tccactatta aagaacgtgg actccaacgt caaagggcga aaaaccgtct
     1981 atcagggcga tggcccacta cgtgaaccat caccctaatc aagttttttg gggtcgaggt
     2041 gccgtaaagc actaaatcgg aaccctaaag ggagcccccg atttagagct tgacggggaa
     2101 agccggcgaa cgtggcgaga aaggaaggga agaaagcgaa aggagcgggc gctagggcgc
     2161 tggcaagtgt agcggtcacg ctgcgcgtaa ccaccacacc cgccgcgctt aatgcgccgc
     2221 tacagggcgc gtcaggtggc acttttcggg gaaatgtgcg cggaacccct atttgtttat
     2281 ttttctaaat acattcaaat atgtatccgc tcatgagaca ataaccctga taaatgcttc
     2341 aataatattg aaaaaggaag agtatgagta ttcaacattt ccgtgtcgcc cttattccct
     2401 tttttgcggc attttgcctt cctgtttttg ctcacccaga aacgctggtg aaagtaaaag
     2461 atgctgaaga tcagttgggt gcacgagtgg gttacatcga actggatctc aacagcggta
     2521 agatccttga gagttttcgc cccgaagaac gttttccaat gatgagcact tttaaagttc
     2581 tgctatgtgg cgcggtatta tcccgtattg acgccgggca agagcaactc ggtcgccgca
     2641 tacactattc tcagaatgac ttggttgagt actcaccagt cacagaaaag catcttacgg
     2701 atggcatgac agtaagagaa ttatgcagtg ctgccataac catgagtgat aacactgcgg
     2761 ccaacttact tctgacaacg atcggaggac cgaaggagct aaccgctttt ttgcacaaca
     2821 tgggggatca tgtaactcgc cttgatcgtt gggaaccgga gctgaatgaa gccataccaa
     2881 acgacgagcg tgacaccacg atgcctgtag caatggcaac aacgttgcgc aaactattaa
     2941 ctggcgaact acttactcta gcttcccggc aacaattaat agactggatg gaggcggata
     3001 aagttgcagg accacttctg cgctcggccc ttccggctgg ctggtttatt gctgataaat
     3061 ctggagccgg tgagcgtggg tctcgcggta tcattgcagc actggggcca gatggtaagc
     3121 cctcccgtat cgtagttatc tacacgacgg ggagtcaggc aactatggat gaacgaaata
     3181 gacagatcgc tgagataggt gcctcactga ttaagcattg gtaactgtca gaccaagttt
     3241 actcatatat actttagatt gatttaaaac ttcattttta atttaaaagg atctaggtga
     3301 agatcctttt tgataatctc atgaccaaaa tcccttaacg tgagttttcg ttccactgag
     3361 cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa
     3421 tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg ccggatcaag
     3481 agctaccaac tctttttccg aaggtaactg gcttcagcag agcgcagata ccaaatactg
     3541 tccttctagt gtagccgtag ttaggccacc acttcaagaa ctctgtagca ccgcctacat
     3601 acctcgctct gctaatcctg ttaccagtgg ctgctgccag tggcgataag tcgtgtctta
     3661 ccgggttgga ctcaagacga tagttaccgg ataaggcgca gcggtcgggc tgaacggggg
     3721 gttcgtgcac acagcccagc ttggagcgaa cgacctacac cgaactgaga tacctacagc
     3781 gtgagctatg agaaagcgcc acgcttcccg aagggagaaa ggcggacagg tatccggtaa
     3841 gcggcagggt cggaacagga gagcgcacga gggagcttcc agggggaaac gcctggtatc
     3901 tttatagtcc tgtcgggttt cgccacctct gacttgagcg tcgatttttg tgatgctcgt
     3961 caggggggcg gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct
     4021 tttgctggcc ttttgctcac atgttctttc ctgcgttatc ccctgattct gtggataacc
     4081 gtattaccgc ctttgagtga gctgataccg ctcgccgcag ccgaacgacc gagcgcagcg
     4141 agtcagtgag cgaggaagcg gaagagcgcc caatacgcaa accgcctctc cccgcgcgtt
     4201 ggccgattca ttaatgcagc tggcacgaca ggtttcccga ctggaaagcg ggcagtgagc
     4261 gcaacgcaat taatgtgagt tagctcactc attaggcacc ccaggcttta cactttatgc
     4321 ttccggctcg tatgttgtgt ggaattgtga gcggataaca attt
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