LOCUS E038pUbiAt-mEGFP 5546 bp DNA circular SYN 26-AUG-2020 DEFINITION Ligation of D360p35s-LmEGFP* into B592pUbiAt-mGFPD-ocs* corrected sequence ACCESSION E038pUbiAt-mEGFP KEYWORDS . SOURCE Unknown. ORGANISM Unknown Unclassified. REFERENCE 1 (bases 1 to 5546) AUTHORS Self JOURNAL Unpublished. COMMENT SECID/File created by Clone Manager, Scientific & Educational Software COMMENT SECNOTES|Vector molecule: B592pUbiAt-mGFPD-ocs* Fragment ends: AflII and MfeI Fragment size: 4910 Insert molecule: D360p35s-LmEGFP* Fragment ends: MfeI and AflII Fragment size: 635 Sequencing primers: GFP-F GACTATCTTCTTCAAGGATGAC FEATURES Location/Qualifiers misc_feature 46..1521 /gene="Pro-Ubq10At" /SECDrawAs="Region" /SECStyleId=1 misc_feature 1117..1120 /product="TATA" /SECDrawAs="Region" /SECStyleId=1 misc_feature 1150..1213 /product="Ubi cds 5" /SECDrawAs="Region" /SECStyleId=1 misc_feature 1216..1519 /product="íntron" /SECDrawAs="Region" /SECStyleId=1 CDS 1547..1925 /gene="mEGFP" /product="monomeric EGFP" /SECDrawAs="Gene" /SECStyleId=1 CDS 1926..2114 /gene="ST-LS1" /product="Intron" /SECDrawAs="Gene" /SECStyleId=1 CDS 2115..2452 /gene="mEGFP" /product="monomeric EGFP" /SECDrawAs="Gene" /SECStyleId=1 misc_feature 2351..2353 /product="A206K - monomeric protein" /SECDrawAs="Region" /SECStyleId=1 misc_feature 2462..2657 /gene="OCS" /product="Terminator" /SECDrawAs="Region" /SECStyleId=1 /SECName="Tocs" /SECDescr="Terminator" misc_feature 3474..4334 /gene="Amp" /product="ORF-1" /SECDrawAs="Region" /SECStyleId=1 /SECName="Amp" /SECDescr="ORF-1" CDS 4377..5093 /gene="ColE1" /product="ColE1" /SECDrawAs="Region" /SECStyleId=1 /SECName="ColE1" /SECDescr="ColE1" ORIGIN 1 gagctctcta gaggccatgg cggccgcact agtggatccc ccgggtttcc ttacattctg 61 agcctctttc cttctaatcc actcatctgc atcttcttgt gtccttacta atacctcatt 121 ggttccaaat tccctccctt taagcaccag ctcgtttctg ttcttccaca gcctcccaag 181 tatccaaggg actaaagcct ccacattctt cagatcagga tattcttgtt taagatgttg 241 aactctatgg aggtttgtat gaactgatga tctaggaccg gataagttcc cttcttcata 301 gcgaacttat tcaaagaatg ttttgtgtat cattcttgtt acattgttat taatgaaaaa 361 atattattgg tcattggact gaacacgagt gttaaatatg gaccaggccc caaataagat 421 ccattgatat atgaattaaa taacaagaat aaatcgagtc accaaaccac ttgccttttt 481 taacgagact tgttcaccaa cttgatacaa aagtcattat cctatgcaaa tcaataatca 541 tacaaaaata tccaataaca ctaaaaaatt aaaagaaatg gataatttca caatatgtta 601 tacgataaag aagttacttt tccaagaaat tcactgattt tataagccca cttgcattag 661 ataaatggca aaaaaaaaca aaaaggaaaa gaaataaagc acgaagaatt ctagaaaata 721 cgaaatacgc ttcaatgcag tgggacccac ggttcaatta ttgccaattt tcagctccac 781 cgtatattta aaaaataaaa cgataatgct aaaaaaatat aaatcgtaac gatcgttaaa 841 tctcaacggc tggatcttat gacgaccgtt agaaattgtg gttgtcgacg agtcagtaat 901 aaacggcgtc aaagtggttg cagccggcac acacgagtcg tgtttatcaa ctcaaagcac 961 aaatactttt cctcaaccta aaaataaggc aattagccaa aaacaacttt gcgtgtaaac 1021 aacgctcaat acacgtgtca ttttattatt agctattgct tcaccgcctt agctttctcg 1081 tgacctagtc gtcctcgtct tttcttcttc ttcttctata aaacaatacc caaagagctc 1141 ttcttcttca caattcagat ttcaatttct caaaatctta aaaactttct ctcaattctc 1201 tctaccgtga tcaaggtaaa tttctgtgtt ccttattctc tcaaaatctt cgattttgtt 1261 ttcgttcgat cccaatttcg tatatgttct ttggtttaga ttctgttaat cttagatcga 1321 agacgatttt ctgggtttga tcgttagata tcatcttaat tctcgattag ggtttcatag 1381 atatcatccg atttgttcaa ataatttgag ttttgtcgaa taattactct tcgatttgtg 1441 atttctatct agatctggtg ttagtttcta gtttgtgcga tcgaatttgt cgattaatct 1501 gagtttttct gattaacagg tacaagctta cgcgtcctcc ataactatgg gcaagggcga 1561 ggaactgttc actggcgtgg tcccaatcct ggtggaactg gatggtgatg tgaacgggca 1621 caagttctcc gtcagcggag agggtgaagg tgatgccacc tacggaaagc tcaccctgaa 1681 gttcatctgc actaccggaa agctccctgt tccgtggcca accctcgtca ccactttcac 1741 ctacggtgtt cagtgcttct cccggtaccc agatcacatg aagcagcatg acttcttcaa 1801 gagcgccatg cccgaaggct acgtgcaaga aaggactatc ttcttcaagg atgacgggaa 1861 ctacaagaca cgtgccgaag tcaagttcga aggtgatacc ctggtgaacc gcatcgagct 1921 gaaaggtaag tttctgcttc tacctttgat atatatataa taattatcat taattagtag 1981 taatataata tttcaaatat ttttttcaaa ataaaagaat gtagtatata gcaattgctt 2041 ttctgtagtt tataagtgtg tatattttaa tttataactt ttctaatata tgaccaaaat 2101 ttgttgatgt gcaggtatcg atttcaagga agatggaaac atcctcggac acaagctgga 2161 gtacaactac aactcccaca acgtatacat catggccgac aagcagaaga acggcatcaa 2221 ggtgaacttc aagatcaggc acaacatcga agatggaagc gtgcaactgg cggaccacta 2281 ccagcagaac acgcccatcg gcgatggccc tgtcctgctg ccggacaacc attacctgtc 2341 cacgcaatct aagctctcca aggaccccaa cgagaagagg gaccacatgg tcctgctgga 2401 gttcgtgacg gctgctggga tcacgcatgg catggatgaa ctctacaagt gacccggtcg 2461 accctgcttt aatgagatat gcgagacgcc tatgatcgca tgatatttgc tttcaattct 2521 gttgtgcacg ttgtaaaaaa cctgagcatg tgtagctcag atccttaccg ccggtttcgg 2581 ttcattctaa tgaatatatc acccgttact atcgtatttt tatgaataat attctccgtt 2641 caatttactg attgtcctcg acaggcctta agggccagat cttgggcccg gtacccaatt 2701 cgccctatag tgagtcgtat tacaattcac tggccgtcgt tttacaacgt cgtgactggg 2761 aaaaccctgg cgttacccaa cttaatcgcc ttgcagcaca tccccctttc gccagctggc 2821 gtaatagcga agaggcccgc accgatcgcc cttcccaaca gttgcgcagc ctgaatggcg 2881 aatggcaaat tgtaagcgtt aatattttgt taaaattcgc gttaaatttt tgttaaatca 2941 gctcattttt taaccaatag gccgaaatcg gcaaaatccc ttataaatca aaagaataga 3001 ccgagatagg gttgagtgtt gttccagttt ggaacaagag tccactatta aagaacgtgg 3061 actccaacgt caaagggcga aaaaccgtct atcagggcga tggcccacta cgtgaaccat 3121 caccctaatc aagttttttg gggtcgaggt gccgtaaagc actaaatcgg aaccctaaag 3181 ggagcccccg atttagagct tgacggggaa agccggcgaa cgtggcgaga aaggaaggga 3241 agaaagcgaa aggagcgggc gctagggcgc tggcaagtgt agcggtcacg ctgcgcgtaa 3301 ccaccacacc cgccgcgctt aatgcgccgc tacagggcgc gtcaggtggc acttttcggg 3361 gaaatgtgcg cggaacccct atttgtttat ttttctaaat acattcaaat atgtatccgc 3421 tcatgagaca ataaccctga taaatgcttc aataatattg aaaaaggaag agtatgagta 3481 ttcaacattt ccgtgtcgcc cttattccct tttttgcggc attttgcctt cctgtttttg 3541 ctcacccaga aacgctggtg aaagtaaaag atgctgaaga tcagttgggt gcacgagtgg 3601 gttacatcga actggatctc aacagcggta agatccttga gagttttcgc cccgaagaac 3661 gttttccaat gatgagcact tttaaagttc tgctatgtgg cgcggtatta tcccgtattg 3721 acgccgggca agagcaactc ggtcgccgca tacactattc tcagaatgac ttggttgagt 3781 actcaccagt cacagaaaag catcttacgg atggcatgac agtaagagaa ttatgcagtg 3841 ctgccataac catgagtgat aacactgcgg ccaacttact tctgacaacg atcggaggac 3901 cgaaggagct aaccgctttt ttgcacaaca tgggggatca tgtaactcgc cttgatcgtt 3961 gggaaccgga gctgaatgaa gccataccaa acgacgagcg tgacaccacg atgcctgtag 4021 caatggcaac aacgttgcgc aaactattaa ctggcgaact acttactcta gcttcccggc 4081 aacaattaat agactggatg gaggcggata aagttgcagg accacttctg cgctcggccc 4141 ttccggctgg ctggtttatt gctgataaat ctggagccgg tgagcgtggg tctcgcggta 4201 tcattgcagc actggggcca gatggtaagc cctcccgtat cgtagttatc tacacgacgg 4261 ggagtcaggc aactatggat gaacgaaata gacagatcgc tgagataggt gcctcactga 4321 ttaagcattg gtaactgtca gaccaagttt actcatatat actttagatt gatttaaaac 4381 ttcattttta atttaaaagg atctaggtga agatcctttt tgataatctc atgaccaaaa 4441 tcccttaacg tgagttttcg ttccactgag cgtcagaccc cgtagaaaag atcaaaggat 4501 cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc 4561 taccagcggt ggtttgtttg ccggatcaag agctaccaac tctttttccg aaggtaactg 4621 gcttcagcag agcgcagata ccaaatactg tccttctagt gtagccgtag ttaggccacc 4681 acttcaagaa ctctgtagca ccgcctacat acctcgctct gctaatcctg ttaccagtgg 4741 ctgctgccag tggcgataag tcgtgtctta ccgggttgga ctcaagacga tagttaccgg 4801 ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac acagcccagc ttggagcgaa 4861 cgacctacac cgaactgaga tacctacagc gtgagctatg agaaagcgcc acgcttcccg 4921 aagggagaaa ggcggacagg tatccggtaa gcggcagggt cggaacagga gagcgcacga 4981 gggagcttcc agggggaaac gcctggtatc tttatagtcc tgtcgggttt cgccacctct 5041 gacttgagcg tcgatttttg tgatgctcgt caggggggcg gagcctatgg aaaaacgcca 5101 gcaacgcggc ctttttacgg ttcctggcct tttgctggcc ttttgctcac atgttctttc 5161 ctgcgttatc ccctgattct gtggataacc gtattaccgc ctttgagtga gctgataccg 5221 ctcgccgcag ccgaacgacc gagcgcagcg agtcagtgag cgaggaagcg gaagagcgcc 5281 caatacgcaa accgcctctc cccgcgcgtt ggccgattca ttaatgcagc tggcacgaca 5341 ggtttcccga ctggaaagcg ggcagtgagc gcaacgcaat taatgtgagt tagctcactc 5401 attaggcacc ccaggcttta cactttatgc ttccggctcg tatgttgtgt ggaattgtga 5461 gcggataaca atttcacaca ggaaacagct atgaccatga ttacgccaag ctcgaaatta 5521 accctcacta aagggaacaa aagctg //