LOCUS       D467pD35s-LmEGFP        4924 bp    DNA     circular SYN 26-AUG-2020
DEFINITION  Ligation of P2x35S* into D360p35s-LmEGFP*
ACCESSION   D467pD35s-LmEGFP
KEYWORDS    .
SOURCE      Unknown.
  ORGANISM  Unknown
            Unclassified.
REFERENCE   1  (bases 1 to 4924)
  AUTHORS   Self
  JOURNAL   Unpublished.
COMMENT     SECID/File created by Clone Manager, Scientific & Educational Software
COMMENT     SECNOTES|Vector molecule:  D360p35s-LmEGFP*
               Fragment ends: BamHI and NcoI
               Fragment size: 4106
            Insert molecule:  P2x35S*
               Fragment ends: NcoI and BamHI
               Fragment size: 818
            Primers for gene synthesis
            PucSyn	CGCCAAGCTCGAAATTAACCCTCAC
            GFPS	GGATTGGGACCACGCCAGTGA
            Sequencing primers
            GFP-seq	CCAGTTCCACCAGGATTG
FEATURES             Location/Qualifiers
     misc_feature    110..894
                     /gene="P35S"
                     /product="P2x35s"
                     /SECDrawAs="Region"
                     /SECStyleId=1
     misc_feature    111..436
                     /product="eh1"
                     /SECDrawAs="Region"
                     /SECStyleId=1
     misc_feature    436..761
                     /product="enh2"
                     /SECDrawAs="Region"
                     /SECStyleId=1
     misc_feature    724
                     /gene="mut "
                     /SECDrawAs="Label"
     misc_feature    822..828
                     /gene="TATA"
                     /SECDrawAs="Label"
     CDS             998..1376
                     /gene="mEGFP"
                     /product="mEGFP"
                     /SECDrawAs="Gene"
                     /SECStyleId=1
     misc_feature    1187..1192
                     /product="F64L/S65T - enhanced fluorescence"
                     /SECDrawAs="Region"
                     /SECStyleId=1
     CDS             1377..1565
                     /gene="ST-LS1"
                     /product="Intron"
                     /SECDrawAs="Gene"
                     /SECStyleId=1
     CDS             1566..1903
                     /gene="mEGFP"
                     /product="mEGFP"
                     /SECDrawAs="Gene"
                     /SECStyleId=1
     misc_feature    1802..1804
                     /product="A206K - monomeric protein"
                     /SECDrawAs="Region"
                     /SECStyleId=1
     misc_feature    1913..2108
                     /gene="OCS"
                     /product="Terminator"
                     /SECDrawAs="Region"
                     /SECStyleId=1
                     /SECName="Tocs"
                     /SECDescr="Terminator"
     misc_feature    2924..3784
                     /gene="Amp"
                     /product="ORF-1"
                     /SECDrawAs="Region"
                     /SECStyleId=1
                     /SECName="Amp"
                     /SECDescr="ORF-1"
     misc_feature    3827..4543
                     /gene="ColE1"
                     /product="ColE1"
                     /SECDrawAs="Region"
                     /SECStyleId=1
                     /SECName="ColE1"
                     /SECDescr="ColE1"
ORIGIN      
        1 cacacaggaa acagctatga ccatgattac gccaagctcg aaattaaccc tcactaaagg
       61 gaacaaaagc tggagctctc tagaggccat ggcggccgct agatcgggcc aacatggtgg
      121 agcacgacac tctcgtctac tccaagaata tcaaagatac agtctcagaa gaccaaaggg
      181 ctattgagac ttttcaacaa agggtaatat cgggaaacct cctcggattc cattgcccag
      241 ctatctgtca cttcatcaaa aggacagtag aaaaggaagg tggcacctac aaatgccatc
      301 attgcgataa aggaaaggct atcgttcaag atgcctctgc cgacagtggt cccaaagatg
      361 gacccccacc cacgaggagc atcgtggaaa aagaagacgt tccaaccacg tcttcaaagc
      421 aagtggattg atgtgaacat ggtggagcac gacactctcg tctactccaa gaatatcaaa
      481 gatacagtct cagaagacca aagggctatt gagacttttc aacaaagggt aatatcggga
      541 aacctcctcg gattccattg cccagctatc tgtcacttca tcaaaaggac agtagaaaag
      601 gaaggtggca cctacaaatg ccatcattgc gataaaggaa aggctatcgt tcaagatgcc
      661 tctgccgaca gtggtcccaa agatggaccc ccacccacga ggagcatcgt ggaaaaagaa
      721 gaccttccaa ccacgtcttc aaagcaagtg gattgatgtg atatctccac tgacgtaagg
      781 gatgacgcac aatcccacta tccttcgcaa gacccttcct ctatataagg aagttcattt
      841 catttggaga ggacacgctg aaatcaccag tctctctcta caaatctatc tctctcgact
      901 agtaggatcc cccgggctgc aggaattcaa gcttacgcgt tgtacaggag taccgccccg
      961 tccggtcctg cccgtcaccg agatcagcgg agagttcatg ggcaagggcg aggaactgtt
     1021 cactggcgtg gtcccaatcc tggtggaact ggatggtgat gtgaacgggc acaagttctc
     1081 cgtcagcgga gagggtgaag gtgatgccac ctacggaaag ctcaccctga agttcatctg
     1141 cactaccgga aagctccctg ttccgtggcc aaccctcgtc accactttca cctacggtgt
     1201 tcagtgcttc tcccgttacc cagatcacat gaagcagcat gacttcttca agagcgccat
     1261 gcccgaaggc tacgtgcaag aaaggactat cttcttcaag gatgacggga actacaagac
     1321 acgtgccgaa gtcaagttcg aaggtgatac cctggtgaac cgcatcgagc tgaaaggtaa
     1381 gtttctgctt ctacctttga tatatatata ataattatca ttaattagta gtaatataat
     1441 atttcaaata tttttttcaa aataaaagaa tgtagtatat agcaattgct tttctgtagt
     1501 ttataagtgt gtatatttta atttataact tttctaatat atgaccaaaa tttgttgatg
     1561 tgcaggtatc gatttcaagg aagatggaaa catcctcgga cacaagctgg agtacaacta
     1621 caactcccac aacgtataca tcatggccga caagcagaag aacggcatca aggtgaactt
     1681 caagatcagg cacaacatcg aagatggaag cgtgcaactg gcggaccact accagcagaa
     1741 cacgcccatc ggcgatggcc ctgtcctgct gccggacaac cattacctgt ccacgcaatc
     1801 taagctctcc aaggacccca acgagaagag ggaccacatg gtcctgctgg agttcgtgac
     1861 ggctgctggg atcacgcatg gcatggatga actctacaag tgacccggtc gaccctgctt
     1921 taatgagata tgcgagacgc ctatgatcgc atgatatttg ctttcaattc tgttgtgcac
     1981 gttgtaaaaa acctgagcat gtgtagctca gatccttacc gccggtttcg gttcattcta
     2041 atgaatatat cacccgttac tatcgtattt ttatgaataa tattctccgt tcaatttact
     2101 gattgtcctc gacaggcctt aagggccaga tcttgggccc ggtacccaat tcgccctata
     2161 gtgagtcgta ttacaattca ctggccgtcg ttttacaacg tcgtgactgg gaaaaccctg
     2221 gcgttaccca acttaatcgc cttgcagcac atcccccttt cgccagctgg cgtaatagcg
     2281 aagaggcccg caccgatcgc ccttcccaac agttgcgcag cctgaatggc gaatggaaat
     2341 tgtaagcgtt aatattttgt taaaattcgc gttaaatttt tgttaaatca gctcattttt
     2401 taaccaatag gccgaaatcg gcaaaatccc ttataaatca aaagaataga ccgagatagg
     2461 gttgagtgtt gttccagttt ggaacaagag tccactatta aagaacgtgg actccaacgt
     2521 caaagggcga aaaaccgtct atcagggcga tggcccacta cgtgaaccat caccctaatc
     2581 aagttttttg gggtcgaggt gccgtaaagc actaaatcgg aaccctaaag ggagcccccg
     2641 atttagagct tgacggggaa agccggcgaa cgtggcgaga aaggaaggga agaaagcgaa
     2701 aggagcgggc gctagggcgc tggcaagtgt agcggtcacg ctgcgcgtaa ccaccacacc
     2761 cgccgcgctt aatgcgccgc tacagggcgc gtcaggtggc acttttcggg gaaatgtgcg
     2821 cggaacccct atttgtttat ttttctaaat acattcaaat atgtatccgc tcatgagaca
     2881 ataaccctga taaatgcttc aataatattg aaaaaggaag agtatgagta ttcaacattt
     2941 ccgtgtcgcc cttattccct tttttgcggc attttgcctt cctgtttttg ctcacccaga
     3001 aacgctggtg aaagtaaaag atgctgaaga tcagttgggt gcacgagtgg gttacatcga
     3061 actggatctc aacagcggta agatccttga gagttttcgc cccgaagaac gttttccaat
     3121 gatgagcact tttaaagttc tgctatgtgg cgcggtatta tcccgtattg acgccgggca
     3181 agagcaactc ggtcgccgca tacactattc tcagaatgac ttggttgagt actcaccagt
     3241 cacagaaaag catcttacgg atggcatgac agtaagagaa ttatgcagtg ctgccataac
     3301 catgagtgat aacactgcgg ccaacttact tctgacaacg atcggaggac cgaaggagct
     3361 aaccgctttt ttgcacaaca tgggggatca tgtaactcgc cttgatcgtt gggaaccgga
     3421 gctgaatgaa gccataccaa acgacgagcg tgacaccacg atgcctgtag caatggcaac
     3481 aacgttgcgc aaactattaa ctggcgaact acttactcta gcttcccggc aacaattaat
     3541 agactggatg gaggcggata aagttgcagg accacttctg cgctcggccc ttccggctgg
     3601 ctggtttatt gctgataaat ctggagccgg tgagcgtggg tctcgcggta tcattgcagc
     3661 actggggcca gatggtaagc cctcccgtat cgtagttatc tacacgacgg ggagtcaggc
     3721 aactatggat gaacgaaata gacagatcgc tgagataggt gcctcactga ttaagcattg
     3781 gtaactgtca gaccaagttt actcatatat actttagatt gatttaaaac ttcattttta
     3841 atttaaaagg atctaggtga agatcctttt tgataatctc atgaccaaaa tcccttaacg
     3901 tgagttttcg ttccactgag cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga
     3961 tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt
     4021 ggtttgtttg ccggatcaag agctaccaac tctttttccg aaggtaactg gcttcagcag
     4081 agcgcagata ccaaatactg tccttctagt gtagccgtag ttaggccacc acttcaagaa
     4141 ctctgtagca ccgcctacat acctcgctct gctaatcctg ttaccagtgg ctgctgccag
     4201 tggcgataag tcgtgtctta ccgggttgga ctcaagacga tagttaccgg ataaggcgca
     4261 gcggtcgggc tgaacggggg gttcgtgcac acagcccagc ttggagcgaa cgacctacac
     4321 cgaactgaga tacctacagc gtgagctatg agaaagcgcc acgcttcccg aagggagaaa
     4381 ggcggacagg tatccggtaa gcggcagggt cggaacagga gagcgcacga gggagcttcc
     4441 agggggaaac gcctggtatc tttatagtcc tgtcgggttt cgccacctct gacttgagcg
     4501 tcgatttttg tgatgctcgt caggggggcg gagcctatgg aaaaacgcca gcaacgcggc
     4561 ctttttacgg ttcctggcct tttgctggcc ttttgctcac atgttctttc ctgcgttatc
     4621 ccctgattct gtggataacc gtattaccgc ctttgagtga gctgataccg ctcgccgcag
     4681 ccgaacgacc gagcgcagcg agtcagtgag cgaggaagcg gaagagcgcc caatacgcaa
     4741 accgcctctc cccgcgcgtt ggccgattca ttaatgcagc tggcacgaca ggtttcccga
     4801 ctggaaagcg ggcagtgagc gcaacgcaat taatgtgagt tagctcactc attaggcacc
     4861 ccaggcttta cactttatgc ttccggctcg tatgttgtgt ggaattgtga gcggataaca
     4921 attt
//
