LOCUS B536pUbiF-ESPS-E 11802 bp DNA circular SYN 04-OCT-2017 DEFINITION Ligation of UbiF-Bsa* into B482p5o2x35s-LH* ACCESSION B536pUbiF-ESPS-E KEYWORDS . SOURCE Unknown. ORGANISM Unknown Unclassified. REFERENCE 1 (bases 1 to 11802) AUTHORS Self JOURNAL Unpublished. COMMENT SECID/File created by Clone Manager, Scientific & Educational Software COMMENT SECNOTES|Vector molecule: B482p5o2x35s-LH* Fragment ends: XhoI and XbaI Fragment size: 9792 Insert molecule: UbiF-Bsa* Fragment ends: BsaI Fragment size: 2010 Primers for gene synthesis UBsaXba CCggtctcactagaCACTAGGCTGCAGT UBsaXho CCggtctcgtcgagtGTACCTGCAGAAGTAAC Sequencing primers LHX1 CTTTATTTCGACGTGTCTAC UF1 CGAGTAGATAATGCCAGCC UF2 GGCCCGGTAGTTCTACTTCT LB FEATURES Location/Qualifiers misc_feature 1..24 /gene="LB" /product="Left Border" /SECDrawAs="Region" /SECStyleId=1 misc_feature 316..2309 /gene="UbiZmF" /SECDrawAs="Region" /SECStyleId=1 CDS 2331..3071 /gene="EPSPS" /translation="MAQINNMAQGIQTLNPNSNFHKPQVPKSSSFLVFGSKKLKNSAN SMLVLKKDSIFMQKFCSFRISASVATACMLHGASSRPATARKSSGLSGTVRIPGDKSI SHRSFMFGGLASGETRITGLLEGEDVINTGKAMQAMGARIRKEGDTWIIDGVGNGGLL APEAPLDFGNAATGCRLTMGLVGVYDFDSTFIGDASLTKRPMGRVLNPLREMGVQVKS EDGDRLPVTLRGPKTPTPITYRVPMASAQ" /SECDrawAs="Gene" /SECStyleId=1 /SECName="EPSPS" misc_feature 3072..3260 /gene="STLS1" /product="Intron STLS1" /SECDrawAs="Gene" /SECStyleId=1 /SECName="Int" /SECDescr="STLS1" CDS 3261..4103 /gene="EPSPS" /codon_start=3 /SECDrawAs="Gene" /SECStyleId=1 misc_feature 4113..4403 /gene="E9" /product="Terminator Rbcs-E9" /SECDrawAs="Region" /SECStyleId=1 misc_feature 4579..4602 /gene="RB" /product="Right Border" /SECDrawAs="Region" /SECStyleId=1 misc_feature 5137..5834 /gene="ColE1" /product="ColE1 ORI" /SECDrawAs="Region" /SECStyleId=1 misc_feature 6383..10088 /gene="pVS1" /product="origin of replication for agrobacteria" /SECDrawAs="Region" /SECStyleId=1 CDS 10272..11282 /gene="Sm/Sp" /product="Strep/Spec resistence" /SECDrawAs="Gene" /SECStyleId=1 ORIGIN 1 ggcaggatat attcaattgt aaatggcttc atgtccggga aatctacatg gatcagcaat 61 gagtatgatg gtcaatatgg agaaaaagaa agagtaatta ccaatttttt ttcaattcaa 121 aaatgtagat gtccgcagcg ttattataaa atgaaagtac attttgataa aacgacaaat 181 tacgatccgt cgtatttata ggcgaaagca ataaacaaat tattctaatt cggaaatctt 241 tatttcgacg tgtctacatt cacgtccaaa tgggggctta gatgagaaac ttcacgatcg 301 gctctagaca ctaggctgca gtgcagcgtg acccggtcgt gcccctctct agagataatg 361 agcattgcat gtctaagtta taaaaaatta ccacatattt tttttgtcac acttgtttga 421 agtgcagttt atctatcttt atacatatat ttaaacttta ctctacgaat aatataatct 481 atagtactac aataatatca gtgttttaga gaatcatata aatgaacagt tagacatggt 541 ctaaaggaca attgagtatt ttgacaacag gactctacag ttttatcttt ttagtgtgca 601 tgtgttctcc tttttttttg caaatagctt cacctatata atacttcatc cattttatta 661 gtacatccat ttagggttta gggttaatgg tttttataga ctaatttttt tagtacatct 721 attttattct attttagcct ctaaattaag aaaactaaaa ctctatttta gtttttttat 781 ttaataattt agatataaaa tagaataaaa taaagtgact aaaaattaaa caaataccct 841 ttaagaaatt aaaaaaacta aggaaacatt tttcttgttt cgagtagata atgccagcct 901 gttaaacgcc gtcgacgagt ctaacggaca ccaaccagcg aaccagcagc gtcgcgtcgg 961 gccaagcgaa gcagacggca cggcatctct gtcgctgcct ctggacccct ctcgagagtt 1021 ccgctccacc gttggacttg ctccgctgtc ggcatccaga aattgcgtgg cggagcggca 1081 gacgtgagcc ggcacggcag gcggcctcct cctcctctca cggcacggca gctacggggg 1141 attcctttcc caccgctcct tcgctttccc ttcctcgccc gccgtaataa atagacaccc 1201 cctccacacc ctctttcccc aacctcgtgt tgttcggagc gcacacacac acaaccagat 1261 ctcccccaaa tccacccgtc ggcacctccg cttcaaggta cgccgctcgt cctccccccc 1321 cccccctctc taccttctct agatcggcgt tccggtccat ggttagggcc cggtagttct 1381 acttctgttc atgtttgtgt tagatccgtg tttgtgttag atccgtgctg ctagcgttcg 1441 tacacggatg cgacctgtac gtcagacacg ttctgattgc taacttgcca gtgtttctct 1501 ttggggaatc ctgggatggc tctagccgtt ccgcagacgg gatcgatttc atgatttttt 1561 ttgtttcgtt gcatagggtt tggtttgccc ttttccttta tttcaatata tgccgtgcac 1621 ttgtttgtcg ggtcatcttt tcatgctttt ttttgtcttg gttgtgatga tgtggtctgg 1681 ttgggcggtc gttctagatc ggagtagaat tctgtttcaa actacctggt ggatttatta 1741 attttggatc tgtatgtgtg tgccatacat attcatagtt acgaattgaa gatgatggat 1801 ggaaatatcg atctaggata ggtatacatg ttgatgcggg ttttactgat gcatatacag 1861 agatgctttt tgttcgcttg gttgtgatga tgtggtgtgg ttgggcggtc gttcattcgt 1921 tctagatcgg agtagaatac tgtttcaaac tacctggtgt atttattaat tttggaactg 1981 tatgtgtgtg tcatacatct tcatagttac gagtttaaga tggatggaaa tatcgatcta 2041 ggataggtat acatgttgat gtgggtttta ctgatgcata tacatgatgg catatgcagc 2101 atctattcat atgctctaac cttgagtacc tatctattat aataaacaag tatgttttat 2161 aattattttg atcttgatat acttggatga tggcatatgc agcagctata tgtggatttt 2221 tttagccctg ccttcatacg ctatttattt gcttggtact gtttcttttg tcgatgctca 2281 ccctgttgtt tggtgttact tctgcaggta cactcgagtc ctccaccgaa atggcccaga 2341 tcaacaacat ggctcagggc attcagaccc tcaaccccaa ttccaacttc cacaagccac 2401 aagtgcctaa gtctagctcc ttcctcgtct tcggctcgaa gaagttgaag aacagcgcca 2461 actccatgct tgttctcaag aaagactcaa tcttcatgca gaagttttgc agcttccgca 2521 tctccgcaag tgtggccact gcttgcatgt tgcacggtgc ctccagccgt ccggcgaccg 2581 ccaggaagtc ttccggcctc tcggggaccg tcagaatccc cggagacaag agcatttccc 2641 accgctcatt catgttcggc ggcctggcca gcggagagac aaggatcacc ggccttctgg 2701 agggtgagga tgtcatcaac accgggaagg ctatgcaggc tatgggagcc cgcatccgga 2761 aggaaggcga cacgtggatt atcgacggcg tgggaaacgg cggtctcctc gccccagagg 2821 cacccttgga cttcggcaac gccgccaccg gatgccgtct gactatgggg cttgttggcg 2881 tgtacgattt cgactccacc ttcatcggag acgcttccct caccaagcgc cctatgggca 2941 gggtcctaaa tccgctccgt gagatgggcg tgcaagtcaa gagcgaggac ggagaccgcc 3001 tgccagttac cttgcgaggc cccaagactc caacacccat cacctacagg gtgcctatgg 3061 cctctgcgca ggtaagtttc tgcttctacc tttgatatat atataataat tatcattaat 3121 tagtagtaat ataatatttc aaatattttt ttcaaaataa aagaatgtag tatatagcaa 3181 ttgcttttct gtagtttata agtgtgtata ttttaattta taacttttct aatatatgac 3241 caaaatttgt tgatgtgcag gtcaagtccg ccgtgctcct tgccgggctg aacactccgg 3301 gtattaccac cgtcatcgag cccattatga ccagagatca taccgagaag atgctccagg 3361 gattcggcgc taacttgacg gttgaaaccg acgccgacgg cgtgcgcacc atccgtctgg 3421 agggaagggg caaactcacc ggccaagtca tcgacgtgcc aggagatccc agctcgactg 3481 cttttccact tgtagccgcc ctcttggtcc ctggttccga cgtgaccatc ctgaacgttc 3541 ttatgaaccc gacccgcacc gggctcatct tgactctcca ggagatgggc gctgacatcg 3601 aagtcattaa ccccaggctg gccggaggcg aggacgtggc agatttgcgc gtccgtagtt 3661 ccaccctgaa gggtgtgaca gttccagagg accgggctcc cagcatgatc gacgagtacc 3721 ctatccttgc cgtcgccgcg gccttcgccg agggcgctac cgtgatgaac ggattggagg 3781 agctgcgcgt caaggagtca gacagactct ccgccgtggc caatgggctc aagctcaacg 3841 gcgttgactg cgatgaggga gaaaccagcc ttgtcgtgcg tggcaggccg gacggcaagg 3901 gactcggtaa cgcttctggg gccgcagtcg ccacgcacct ggaccaccgc atcgcgatgt 3961 ccttcctcgt gatgggcctt gtttcggaga acccagtcac cgtggacgat gccaccatga 4021 ttgctactag cttccccgag ttcatggact tgatggccgg actgggagcc aagatcgagc 4081 tgtccgacac caaggctgcc taacgcgttc gagtattatg gcattgggaa aactgttttt 4141 cttgtaccat ttgttgtgct tgtaatttac tgtgtttttt attcggtttt cgctatcgaa 4201 ctgtgaaatg gaaatggatg gagaagagtt aatgaatgat atggtccttt tgttcattct 4261 caaattaata ttatttgttt tttctcttat ttgttgtgtg ttgaatttga aattataaga 4321 gatatgcaaa cattttgttt tgagtaaaaa tgtgtcaaat cgtggcctct aatgaccgaa 4381 gttaatatga ggagtaaaac actgaagcct gcaggcatgc aagctgatcc actagaggcc 4441 atggcggccg cactagtgga tcccccggga tgcatgaatt caagcttatc gatgtcgaca 4501 ggccttaagg gccagatctt gggcccggta cccgatcaga ttgtcgtttc ccgccttcgg 4561 tttaaactat cagtgtttga caggatatat tggcgggtaa acctaagaga aaagagcgtt 4621 tattagaata atcggatatt taaaagggcg tgaaaaggtt tatccgttcg tccatttgta 4681 tgtgcatgcc aaccacaggg ttcccctcgg gagtgcttgg cattccgtgc gataatgact 4741 tctgttcaac cacccaaacg tcggaaagcc tgacgacgga gcagcattcc aaaaagatcc 4801 cttggctcgt ctgggtcggc tagaaggtcg agtgggctgc tgtggcttga tccctcaacg 4861 cggtcgcgga cgtagcgcag cgccgaaaaa tcctcgatcg caaatccgac gctgtcgaaa 4921 agcgtgatct gcttgtcgct ctttcggccg acgtcctggc cagtcatcac gcgccaaagt 4981 tccgtcacag gatgatctgg cgcgagttgc tggatctcgc cttcaatccg ggtctgtggc 5041 gggaactcca cgaaaatatc cgaacgcagc aagatatcgc ggtgcatctc ggtcttgcct 5101 gggcagtcgc cgccgacgcc gttgatgtgg acgccgaaaa ggatctaggt gaagatcctt 5161 tttgataatc tcatgaccaa aatcccttaa cgtgagtttt cgttccactg agcgtcagac 5221 cccgtagaaa agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc 5281 ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca 5341 actctttttc cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgttcttcta 5401 gtgtagccgt agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct 5461 ctgctaatcc tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg 5521 gactcaagac gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc 5581 acacagccca gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta 5641 tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg 5701 gtcggaacag gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt 5761 cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg 5821 cggagcctat ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg 5881 ccttttgctc acatgttctt tcctgcgtta tcccctgatt ctgtggataa ccgattaccg 5941 cctttgagtg agctgatacc gctcgccgca gccgaacgac cgagcgcagc gagtcagtga 6001 gcgaggaagc ggaagagcgc ctgatgcggt attttctcct tacgcatctg tgcggtattt 6061 cacaccgcat atggtgcact ctcagtacaa tctgctctga tgccgcatag ttaagccagt 6121 atacactccg ctatcgctac gtgactgggt catggctgcg ccccgacacc cgccaacacc 6181 cgctgacgcg ccctgacggg cttgtctgct cccggcatcc gcttacagac aagctgtgac 6241 cgtctccggg agctgcatgt gtcagaggtt ttcaccgtca tcaccgaaac gcgcgaggca 6301 ggggtacgtc gaggtcgatc caacccctcc gctgctatag tgcagtcggc ttctgacgtt 6361 cagtgcagcc gtcttctgaa aacgacatgt cgcacaagtc ctaagttacg cgacaggctg 6421 ccgccctgcc cttttcctgg cgttttcttg tcgcgtgttt tagtcgcata aagtagaata 6481 cttgcgacta gaaccggaga cattacgcca tgaacaagag cgccgccgct ggcctgctgg 6541 gctatgcccg cgtcagcacc gacgaccagg acttgaccaa ccaacgggcc gaactgcacg 6601 cggccggctg caccaagctg ttttccgaga agatcaccgg caccaggcgc gaccgcccgg 6661 agctggccag gatgcttgac cacctacgcc ctggcgacgt tgtgacagtg accaggctag 6721 accgcctggc ccgcagcacc cgcgacctac tggacattgc cgagcgcatc caggaggccg 6781 gcgcgggcct gcgtagcctg gcagagccgt gggccgacac caccacgccg gccggccgca 6841 tggtgttgac cgtgttcgcc ggcattgccg agttcgagcg ttccctaatc atcgaccgca 6901 cccggagcgg gcgcgaggcc gccaaggcgc gaggcgtgaa gtttggcccc cgccctaccc 6961 tcaccccggc acagatcgcg cacgcccgcg agctgatcga ccaggaaggc cgcaccgtga 7021 aagaggcggc tgcactgctt ggcgtgcatc gctcgaccct gtaccgcgca cttgagcgca 7081 gcgaggaagt gacgcccacc gaggccaggc ggcgcggtgc cttccgtgag gacgcattga 7141 ccgaggccga cgccctggcg gccgccgaga atgaacgcca agaggaacaa gcatgaaacc 7201 gcaccaggac ggccaggacg aaccgttttt cattaccgaa gagatcgagg cggagatgat 7261 cgcggccggg tacgtgttcg agccgcccgc gcacgtctca accgtgcggc tgcatgaaat 7321 cctggccggt ttgtctgatg ccaagctcgc ggcctggccg gcgagcttgg ccgctgaaga 7381 aaccgagcgc cgccgtctaa aaaggtgatg tgtatttgag taaaacagct tgcgtcatgc 7441 ggtcgctgcg tatatgatgc gatgagtaaa taaacaaata cgcaagggga acgcatgaag 7501 gttatcgctg tacttaacca gaaaggcggg tcaggcaaga cgaccatcgc aacccatcta 7561 gcccgcgccc tgcaactcgc cggggccgat gttctgttag tcgattccga tccccagggc 7621 agtgcccgcg attgggcggc cgtgcgggaa gatcaaccgc taaccgttgt cggcatcgac 7681 cgcccgacga ttgaccgcga cgtgaaggcc atcggccggc gcgacttcgt agtgatcgac 7741 ggagcgcccc aggcggcgga cttggctgtg tccgcgatca aggcagccga cttcgtgctg 7801 attccggtgc agccaagccc ttacgacata tgggccaccg ccgacctggt ggagctggtt 7861 aagcagcgca ttgaggtcac ggatggaagg ctacaagcgg cctttgtcgt gtcgcgggcg 7921 atcaaaggca cgcgcatcgg cggtgaggtt gccgaggcgc tggccgggta cgagctgccc 7981 attcttgagt cccgtatcac gcagcgcgtg agctacccag gcactgccgc cgccggcaca 8041 accgttcttg aatcagaacc cgagggcgac gctgcccgcg aggtccaggc gctggccgct 8101 gaaattaaat caaaactcat ttgagttaat gaggtaaaga gaaaatgagc aaaagcacaa 8161 acacgctaag tgccggccgt ccgagcgcac gcagcagcaa ggctgcaacg ttggccagcc 8221 tggcagacac gccagccatg aagcgggtca actttcagtt gccggcggag gatcacacca 8281 agctgaagat gtacgcggta cgccaaggca agaccattac cgagctgcta tctgaataca 8341 tcgcgcagct accagagtaa atgagcaaat gaataaatga gtagatgaat tttagcggct 8401 aaaggaggcg gcatggaaaa tcaagaacaa ccaggcaccg acgccgtgga atgccccatg 8461 tgtggaggaa cgggcggttg gccaggcgta agcggctggg ttgtctgccg gccctgcaat 8521 ggcactggaa cccccaagcc cgaggaatcg gcgtgagcgg tcgcaaacca tccggcccgg 8581 tacaaatcgg cgcggcgctg ggtgatgacc tggtggagaa gttgaaggcg gcgcaggccg 8641 cccagcggca acgcatcgag gcagaagcac gccccggtga atcgtggcaa gcggccgctg 8701 atcgaatccg caaagaatcc cggcaaccgc cggcagccgg tgcgccgtcg attaggaagc 8761 cgcccaaggg cgacgagcaa ccagattttt tcgttccgat gctctatgac gtgggcaccc 8821 gcgatagtcg cagcatcatg gacgtggccg ttttccgtct gtcgaagcgt gaccgacgag 8881 ctggcgaggt gatccgctac gagcttccag acgggcacgt agaggtttcc gcagggccgg 8941 ccggcatggc gagtgtgtgg gattacgacc tggtactgat ggcggtttcc catctaaccg 9001 aatccatgaa ccgataccgg gaagggaagg gagacaagcc cggccgcgtg ttccgtccac 9061 acgttgcgga cgtactcaag ttctgccggc gagccgatgg cggaaagcag aaagacgacc 9121 tggtagaaac ctgcattcgg ttaaacacca cgcacgttgc catgcagcgt acgaagaagg 9181 ccaagaacgg ccgcctggtg acggtatccg agggtgaagc cttgattagc cgctacaaga 9241 tcgtaaagag cgaaaccggg cggccggagt acatcgagat cgagctagct gattggatgt 9301 accgcgagat cacagaaggc aagaacccgg acgtgctgac ggttcacccc gattactttt 9361 tgatcgatcc cggcatcggc cgttttctct accgcctggc acgccgcgcc gcaggcaagg 9421 cagaagccag atggttgttc aagacgatct acgaacgcag tggcagcgcc ggagagttca 9481 agaagttctg tttcaccgtg cgcaagctga tcgggtcaaa tgacctgccg gagtacgatt 9541 tgaaggagga ggcggggcag gctggcccga tcctagtcat gcgctaccgc aacctgatcg 9601 agggcgaagc atccgccggt tcctaatgta cggagcagat gctagggcaa attgccctag 9661 caggggaaaa aggtcgaaaa ggtctctttc ctgtggatag cacgtacatt gggaacccaa 9721 agccgtacat tgggaaccgg aacccgtaca ttgggaaccc aaagccgtac attgggaacc 9781 ggtcacacat gtaagtgact gatataaaag agaaaaaagg cgatttttcc gcctaaaact 9841 ctttaaaact tattaaaact cttaaaaccc gcctggcctg tgcataactg tctggccagc 9901 gcacagccga agagctgcaa aaagcgccta cccttcggtc gctgcgctcc ctacgccccg 9961 ccgcttcgcg tcggcctatc gcggccgctg gccgctcaaa aatggctggc ctacggccag 10021 gcaatctacc agggcgcgga caagccgcgc cgtcgccact cgaccgccgg cgcccacatc 10081 aaggcaccgg tgggtatgcc tgacgatgcg tggagaccga aaccttgcgc tcgttcgcca 10141 gccaggacag aaatgcctcg acttcgctgc tgcccaaggt tgccgggtga cgcacaccgt 10201 ggaaacggat gaaggcacga acccagtgga cataagcctg ttcggttcgt aagctgtaat 10261 gcaagtagcg tatgcgctca cgcaactggt ccagaacctt gaccgaacgc agcggtggta 10321 acggcgcagt ggcggttttc atggcttgtt atgactgttt ttttggggta cagtctatgc 10381 ctcgggcatc caagcagcaa gcgcgttacg ccgtgggtcg atgtttgatg ttatggagca 10441 gcaacgatgt tacgcagcag ggcagtcgcc ctaaaacaaa gttaaacatc atgagggaag 10501 cggtgatcgc cgaagtatcg actcaactat cagaggtagt tggcgtcatc gagcgccatc 10561 tcgaaccgac gttgctggcc gtacatttgt acggctccgc agtggatggc ggcctgaagc 10621 cacacagtga tattgatttg ctggttacgg tgaccgtaag gcttgatgaa acaacgcggc 10681 gagctttgat caacgacctt ttggaaactt cggcttcccc tggagagagc gagattctcc 10741 gcgctgtaga agtcaccatt gttgtgcacg acgacatcat tccgtggcgt tatccagcta 10801 agcgcgaact gcaatttgga gaatggcagc gcaatgacat tcttgcaggt atcttcgagc 10861 cagccacgat cgacattgat ctggctatct tgctgacaaa agcaagagaa catagcgttg 10921 ccttggtagg tccagcggcg gaggaactct ttgatccggt tcctgaacag gatctatttg 10981 aggcgctaaa tgaaacctta acgctatgga actcgccgcc cgactgggct ggcgatgagc 11041 gaaatgtagt gcttacgttg tcccgcattt ggtacagcgc agtaaccggc aaaatcgcgc 11101 cgaaggatgt cgctgccgac tgggcaatgg agcgcctgcc ggcccagtat cagcccgtca 11161 tacttgaagc tagacaggct tatcttggac aagaagaaga tcgcttggcc tcgcgcgcag 11221 atcagttgga agaatttgtc cactacgtga aaggcgagat caccaaggta gtcggcaaat 11281 aatgtctaac aattcgttca agccgacgcc gcttcgcggc gcggcttaac tcaagcgtta 11341 gatgcactaa gcacataatt gctcacagcc aaactatcag gtcaagtctg cttttattat 11401 ttttaagcgt gcataataag ccctacacaa attgggagat atatcatgaa aggctggctt 11461 tttcttgtta tcgcaatagt tggcgaagta atcgcaacat agcttgcttg gtcgttccgc 11521 gtgaacgtcg gctcgattgt acctgcgttc aaatactttg cgatcgtgtt gcgcgcctgc 11581 ccggtgcgtc ggctgatctc acggatcgac tgcttctctc gcaacgccat ccgacggatg 11641 atgtttaaaa gtcccatgtg gatcactccg ttgccccgtc gctcaccgtg ttggggggaa 11701 ggtgcacatg gctcagttct caatggaaat tatctgccta accggctcag ttctgcgtag 11761 aaaccaacat gcaagctcca ccgggtgcaa agcggcagcg gc //