LOCUS       B728pUbiZm-YFPiM        5583 bp    DNA     circular SYN 04-OCT-2017
DEFINITION  Ligation of PCR1* into B595pUbiZm-mGFPM-ocs*
ACCESSION   B728pUbiZm-YFPiM
KEYWORDS    .
SOURCE      Unknown.
  ORGANISM  Unknown
            Unclassified.
REFERENCE   1  (bases 1 to 5583)
  AUTHORS   Self
  JOURNAL   Unpublished.
COMMENT     SECID/File created by Clone Manager, Scientific & Educational Software
COMMENT     SECNOTES|Vector molecule:  B595pUbiZm-mGFPM-ocs*
               Fragment ends: SalI and MluI
               Fragment size: 4654
            Insert molecule:  PCR1*
               Fragment ends: MluI and SalI
               Fragment size: 929
            Sequencing primers:
            Ubim
            M13FL
            pUCF	GCAAGGCGATTAAGTTGGGTA optional statt M13FL
FEATURES             Location/Qualifiers
     misc_feature    122..1629
                     /gene="pUbi"
                     /product="Promotor"
                     /SECDrawAs="Region"
                     /SECStyleId=1
     misc_feature    1108..1627
                     /gene="Int"
                     /product="Intron"
                     /SECDrawAs="Region"
                     /SECStyleId=1
     CDS             1655..2036
                     /gene="YFP'"
                     /product="mCtrine (codon optimized for plants)"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLT
                     LKFICTTGKLPVPWPTLVTTFGYGLMCFARYPDHMKQHDFFKSAMPEGYVQERTIFFK
                     DDGNYKTRAEVKFEGDTLVNRIELK"
                     /SECDrawAs="Gene"
                     /SECStyleId=1
                     /SECName="mCitrine "
                     /SECDescr="mCitrine (codon optimized for plants)"
     CDS             2037..2225
                     /gene="Int"
                     /product="ST-LS1"
                     /SECDrawAs="Gene"
                     /SECStyleId=1
     CDS             2226..2563
                     /gene="'YFP"
                     /product="mCtrine (codon optimized for plants)"
                     /codon_start=2
                     /translation="YRFQGRWKYSRTQVGVQLQQP"
                     /SECDrawAs="Gene"
                     /SECStyleId=1
                     /SECName="mCitrine "
                     /SECDescr="mCitrine (codon optimized for plants)"
     misc_feature    2572..2767
                     /gene="OCS"
                     /product="Terminator"
                     /SECDrawAs="Region"
                     /SECStyleId=1
                     /SECName="OCS"
                     /SECDescr="Terminator"
     misc_feature    3583..4443
                     /gene="Amp"
                     /product="ORF-1"
                     /SECDrawAs="Gene"
                     /SECStyleId=1
                     /SECName="Amp"
                     /SECDescr="ORF-1"
     CDS             4486..5202
                     /gene="ColE1"
                     /product="ColE1"
                     /SECDrawAs="Region"
                     /SECStyleId=1
                     /SECName="ColE1"
                     /SECDescr="ColE1"
ORIGIN      
        1 cacacaggaa acagctatga ccatgattac gccaagctcg aaattaaccc tcactaaagg
       61 gaacaaaagc tggagctctc tagaggccat ggcggccgca ctagtggatc ccccgggtac
      121 gtgcagtgca gcgtgacccg gtcgtgcccc tctctagaga taatgagcat tgcatgtcta
      181 agttataaaa aattaccaca tatttttttt gtcacacttg tttgaagtgc agtttatcta
      241 tctttataca tatatttaaa ctttactcta cgaataatat aatctatagt actacaataa
      301 tatcagtgtt ttagagaatc atataaatga acagttagac atggtctaaa ggacaattga
      361 gtattttgac aacaggactc tacagtttta tctttttagt gtgcatgtgt tctccttttt
      421 ttttgcaaat agcttcacct atataatact tcatccattt tattagtaca tccatttagg
      481 gtttagggtt aatggttttt atagactaat ttttttagta catctatttt attctatttt
      541 agcctctaaa ttaagaaaac taaaactcta ttttagtttt tttatttaat aatttagata
      601 taaaatagaa taaaataaag tgactaaaaa ttaaacaaat accctttaag aaattaaaaa
      661 aactaaggaa acatttttct tgtttcgagt agataatgcc agcctgttaa acgccgtcga
      721 tcgacgagtc taacggacac caaccagcga accagcagcg tcgcgtcggg ccaagcgaag
      781 cagacggcac ggcatctctg tcgctgcctc tggacccctc tcgagagttc cgctccaccg
      841 ttggacttgc tccgctgtcg gcatccagaa attgcgtggc ggagcggcag acgtgagccg
      901 gcacggcagg cggcctcctc ctcctctcac ggcaccggca gctacggggg attcctttcc
      961 caccgctcct tcgctttccc ttcctcgccc gccgtaataa atagacaccc cctccacacc
     1021 ctctttcccc aacctcgtgt tgttcggagc gcacacacac acaaccagat ctcccccaaa
     1081 tccacccgtc ggcacctccg cttcaaggta cgccgctcgt cctccccccc cccccctctc
     1141 taccttctct agatcggcgt tccggtccat ggttagggcc cggtagttct acttctgttc
     1201 atgtttgtgt tagatccgtg tttgtgttag atccgtgctg ctagcgttcg tacacggatg
     1261 cgacctgtac gtcagacacg ttctgattgc taacttgcca gtgtttctct ttggggaatc
     1321 ctgggatggc tctagccgtt ccgcagacgg gatcgatcta ggataggtat acatgttgat
     1381 gtgggtttta ctgatgcata tacatgatgg catatgcagc atctattcat atgctctaac
     1441 cttgagtacc tatctattat aataaacaag tatgttttat aattattttg atcttgatat
     1501 acttggatga tggcatatgc agcagctata tgtggatttt tttagccctg ccttcatacg
     1561 ctatttattt gcttggtact gtttcttttg tcgatgctca ccctgttgtt tggtgttact
     1621 tctgcaggta caagcttacg cgtcctccac cacaatggtg tccaagggcg aggagctgtt
     1681 caccggagtc gttcccatcc tcgtcgaatt ggacggcgac gtgaacggtc acaagttcag
     1741 cgtgtctggg gagggcgagg gagatgccac ctacggcaag ctcactctca agttcatttg
     1801 caccaccgga aagctgccag tcccttggcc gaccctcgtt actaccttcg gctacggttt
     1861 gatgtgcttc gctcgctacc ccgaccacat gaagcagcac gacttcttta agtccgccat
     1921 gccagagggc tacgtccaag agaggaccat cttcttcaag gacgatggga actacaagac
     1981 ccgcgccgaa gtgaagttcg agggcgacac cctggtgaac cgcatcgagc tgaaaggtaa
     2041 gtttctgctt ctacctttga tatatatata ataattatca ttaattagta gtaatataat
     2101 atttcaaata tttttttcaa aataaaagaa tgtagtatat agcaattgct tttctgtagt
     2161 ttataagtgt gtatatttta atttataact tttctaatat atgaccaaaa tttgttgatg
     2221 tgcaggtatc gatttcaagg aagatggaaa tattctcgga cacaagttgg agtacaacta
     2281 caacagccat aacgtgtata tcatggctga caagcagaag aacggcatca aagtcaactt
     2341 caagatcagg cacaatattg aggatggttc ggtgcagctc gccgaccact accagcaaaa
     2401 cactcctatc ggggacggcc cggttcttct ccccgacaac cactacctgt cctaccagag
     2461 cgccctctcc aaggacccaa acgaaaagcg cgatcacatg gtcttgcttg agttcgtgac
     2521 cgctgccgga atcaccctgg gcatggacga gctttacaag tgactcgtcg accctgcttt
     2581 aatgagatat gcgagacgcc tatgatcgca tgatatttgc tttcaattct gttgtgcacg
     2641 ttgtaaaaaa cctgagcatg tgtagctcag atccttaccg ccggtttcgg ttcattctaa
     2701 tgaatatatc acccgttact atcgtatttt tatgaataat attctccgtt caatttactg
     2761 attgtcctcg acaggcctta agggccagat cttgggcccg gtacccaatt cgccctatag
     2821 tgagtcgtat tacaattcac tggccgtcgt tttacaacgt cgtgactggg aaaaccctgg
     2881 cgttacccaa cttaatcgcc ttgcagcaca tccccctttc gccagctggc gtaatagcga
     2941 agaggcccgc accgatcgcc cttcccaaca gttgcgcagc ctgaatggcg aatggaaatt
     3001 gtaagcgtta atattttgtt aaaattcgcg ttaaattttt gttaaatcag ctcatttttt
     3061 aaccaatagg ccgaaatcgg caaaatccct tataaatcaa aagaatagac cgagataggg
     3121 ttgagtgttg ttccagtttg gaacaagagt ccactattaa agaacgtgga ctccaacgtc
     3181 aaagggcgaa aaaccgtcta tcagggcgat ggcccactac gtgaaccatc accctaatca
     3241 agttttttgg ggtcgaggtg ccgtaaagca ctaaatcgga accctaaagg gagcccccga
     3301 tttagagctt gacggggaaa gccggcgaac gtggcgagaa aggaagggaa gaaagcgaaa
     3361 ggagcgggcg ctagggcgct ggcaagtgta gcggtcacgc tgcgcgtaac caccacaccc
     3421 gccgcgctta atgcgccgct acagggcgcg tcaggtggca cttttcgggg aaatgtgcgc
     3481 ggaaccccta tttgtttatt tttctaaata cattcaaata tgtatccgct catgagacaa
     3541 taaccctgat aaatgcttca ataatattga aaaaggaaga gtatgagtat tcaacatttc
     3601 cgtgtcgccc ttattccctt ttttgcggca ttttgccttc ctgtttttgc tcacccagaa
     3661 acgctggtga aagtaaaaga tgctgaagat cagttgggtg cacgagtggg ttacatcgaa
     3721 ctggatctca acagcggtaa gatccttgag agttttcgcc ccgaagaacg ttttccaatg
     3781 atgagcactt ttaaagttct gctatgtggc gcggtattat cccgtattga cgccgggcaa
     3841 gagcaactcg gtcgccgcat acactattct cagaatgact tggttgagta ctcaccagtc
     3901 acagaaaagc atcttacgga tggcatgaca gtaagagaat tatgcagtgc tgccataacc
     3961 atgagtgata acactgcggc caacttactt ctgacaacga tcggaggacc gaaggagcta
     4021 accgcttttt tgcacaacat gggggatcat gtaactcgcc ttgatcgttg ggaaccggag
     4081 ctgaatgaag ccataccaaa cgacgagcgt gacaccacga tgcctgtagc aatggcaaca
     4141 acgttgcgca aactattaac tggcgaacta cttactctag cttcccggca acaattaata
     4201 gactggatgg aggcggataa agttgcagga ccacttctgc gctcggccct tccggctggc
     4261 tggtttattg ctgataaatc tggagccggt gagcgtgggt ctcgcggtat cattgcagca
     4321 ctggggccag atggtaagcc ctcccgtatc gtagttatct acacgacggg gagtcaggca
     4381 actatggatg aacgaaatag acagatcgct gagataggtg cctcactgat taagcattgg
     4441 taactgtcag accaagttta ctcatatata ctttagattg atttaaaact tcatttttaa
     4501 tttaaaagga tctaggtgaa gatccttttt gataatctca tgaccaaaat cccttaacgt
     4561 gagttttcgt tccactgagc gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat
     4621 cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct accagcggtg
     4681 gtttgtttgc cggatcaaga gctaccaact ctttttccga aggtaactgg cttcagcaga
     4741 gcgcagatac caaatactgt ccttctagtg tagccgtagt taggccacca cttcaagaac
     4801 tctgtagcac cgcctacata cctcgctctg ctaatcctgt taccagtggc tgctgccagt
     4861 ggcgataagt cgtgtcttac cgggttggac tcaagacgat agttaccgga taaggcgcag
     4921 cggtcgggct gaacgggggg ttcgtgcaca cagcccagct tggagcgaac gacctacacc
     4981 gaactgagat acctacagcg tgagctatga gaaagcgcca cgcttcccga agggagaaag
     5041 gcggacaggt atccggtaag cggcagggtc ggaacaggag agcgcacgag ggagcttcca
     5101 gggggaaacg cctggtatct ttatagtcct gtcgggtttc gccacctctg acttgagcgt
     5161 cgatttttgt gatgctcgtc aggggggcgg agcctatgga aaaacgccag caacgcggcc
     5221 tttttacggt tcctggcctt ttgctggcct tttgctcaca tgttctttcc tgcgttatcc
     5281 cctgattctg tggataaccg tattaccgcc tttgagtgag ctgataccgc tcgccgcagc
     5341 cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg aagagcgccc aatacgcaaa
     5401 ccgcctctcc ccgcgcgttg gccgattcat taatgcagct ggcacgacag gtttcccgac
     5461 tggaaagcgg gcagtgagcg caacgcaatt aatgtgagtt agctcactca ttaggcaccc
     5521 caggctttac actttatgct tccggctcgt atgttgtgtg gaattgtgag cggataacaa
     5581 ttt
//
