LOCUS       B812p35s-LNeon          4358 bp    DNA     circular SYN 04-OCT-2017
DEFINITION  Ligation of neon-mlu-sal* into B629p35s-LGFP*
ACCESSION   B812p35s-LNeon
KEYWORDS    .
SOURCE      Unknown.
  ORGANISM  Unknown
            Unclassified.
REFERENCE   1  (bases 1 to 4358)
  AUTHORS   Self
  JOURNAL   Unpublished.
COMMENT     SECID/File created by Clone Manager, Scientific & Educational Software
COMMENT     SECNOTES|Vector molecule:  B629p35s-LGFP*
               Fragment ends: SalI and MluI
               Fragment size: 3580
            Insert molecule:  neon-mlu-sal*
               Fragment ends: MluI and SalI
               Fragment size: 778
            Genesynthesis
            Sequencing primers:
            35RT	CTTCGCAAGACCCTTCCTCT     GC rich
FEATURES             Location/Qualifiers
     misc_feature    106..528
                     /gene="35S-Pro"
                     /product="Promotor-Region"
                     /SECDrawAs="Region"
                     /SECStyleId=1
                     /SECName="35S-Pro"
                     /SECDescr="Promotor-Region"
     misc_feature    576..626
                     /gene="linker"
                     /SECDrawAs="Region"
                     /SECStyleId=1
     CDS             627..1337
                     /gene="Neon"
                     /SECDrawAs="Gene"
                     /SECStyleId=1
     misc_feature    1347..1542
                     /gene="OCS"
                     /product="Terminator"
                     /SECDrawAs="Region"
                     /SECStyleId=1
                     /SECName="OCS"
                     /SECDescr="Terminator"
     misc_feature    2358..3218
                     /gene="Amp"
                     /product="ORF-1"
                     /SECDrawAs="Gene"
                     /SECStyleId=1
                     /SECName="Amp"
                     /SECDescr="ORF-1"
     misc_feature    3261..3977
                     /gene="ColE1"
                     /product="ColE1"
                     /SECDrawAs="Region"
                     /SECStyleId=1
                     /SECName="ColE1"
                     /SECDescr="ColE1"
ORIGIN      
        1 cacacaggaa acagctatga ccatgattac gccaagctcg aaattaaccc tcactaaagg
       61 gaacaaaagc tggagctctc tagaggccat ggcggccgca ctagatcaac atggtggagc
      121 acgacactct cgtctactcc aagaatatca aagatacagt ctcagaagac cagagggcta
      181 ttgagacttt tcaacaaagg gtaatatcgg gaaacctcct cggattccat tgcccagcta
      241 tctgtcactt catcgaaagg acagtagaaa aggaagatgg cttctacaaa tgccatcatt
      301 gcgataaagg aaaggctatc gttcaagatg cctctaccga cagtggtccc aaagatggac
      361 ccccacccac gaggaacatc gtggaaaaag aagacgttcc aaccacgtct tcaaagcaag
      421 tggattgatg tgatatctcc actgacgtaa gggatgacgc acaatcccac tatccttcgc
      481 aagacccttc ctctatataa ggaagttcat ttcatttgga gaggacacta gttggatccc
      541 ccgggctgca ggaattcaag cttacgcgtt gtacaggagt accgccccgt ccggtcctgc
      601 ccgtcaccga gatcagcgga gagttcatgg tgtccaaggg cgaggaggac aacatggcca
      661 gcctccccgc tacccacgag ttgcacatct tcggatctat taacggtgtt gacttcgata
      721 tggtcggcca ggggaccggc aacccaaatg acggatacga agagctgaac ctcaagtcca
      781 ctaagggaga cctccaattc tcgccttgga tcttggtgcc gcacatcgga tacggcttcc
      841 atcagtacct cccctaccca gacggtatga gccccttcca ggccgctatg gtcgatgggt
      901 ccggctacca agtgcaccgc accatgcagt ttgaggacgg agcctcactg acagtcaact
      961 acagatacac ctatgagggc tcccacatca agggagaggc ccaagtgaag ggcacgggtt
     1021 tcccagcaga cggccctgtt atgaccaaca gcctcaccgc ggccgactgg tgccgttcta
     1081 agaagaccta cccgaacgat aagactatta tctccacctt caaatggtcg tacaccacag
     1141 ggaacggaaa gcgctacagg agcaccgcta ggaccacgta caccttcgcc aagccaatgg
     1201 ccgctaatta ccttaagaac cagcccatgt acgtcttccg caagactgag ttgaagcact
     1261 ccaagaccga actgaacttc aaggagtggc agaaggcttt caccgacgtg atgggcatgg
     1321 acgagcttta caagtgaccc ggtcgaccct gctttaatga gatatgcgag acgcctatga
     1381 tcgcatgata tttgctttca attctgttgt gcacgttgta aaaaacctga gcatgtgtag
     1441 ctcagatcct taccgccggt ttcggttcat tctaatgaat atatcacccg ttactatcgt
     1501 atttttatga ataatattct ccgttcaatt tactgattgt cctcgacagg ccttaagggc
     1561 cagatcttgg gcccggtacc caattcgccc tatagtgagt cgtattacaa ttcactggcc
     1621 gtcgttttac aacgtcgtga ctgggaaaac cctggcgtta cccaacttaa tcgccttgca
     1681 gcacatcccc ctttcgccag ctggcgtaat agcgaagagg cccgcaccga tcgcccttcc
     1741 caacagttgc gcagcctgaa tggcgaatgg aaattgtaag cgttaatatt ttgttaaaat
     1801 tcgcgttaaa tttttgttaa atcagctcat tttttaacca ataggccgaa atcggcaaaa
     1861 tcccttataa atcaaaagaa tagaccgaga tagggttgag tgttgttcca gtttggaaca
     1921 agagtccact attaaagaac gtggactcca acgtcaaagg gcgaaaaacc gtctatcagg
     1981 gcgatggccc actacgtgaa ccatcaccct aatcaagttt tttggggtcg aggtgccgta
     2041 aagcactaaa tcggaaccct aaagggagcc cccgatttag agcttgacgg ggaaagccgg
     2101 cgaacgtggc gagaaaggaa gggaagaaag cgaaaggagc gggcgctagg gcgctggcaa
     2161 gtgtagcggt cacgctgcgc gtaaccacca cacccgccgc gcttaatgcg ccgctacagg
     2221 gcgcgtcagg tggcactttt cggggaaatg tgcgcggaac ccctatttgt ttatttttct
     2281 aaatacattc aaatatgtat ccgctcatga gacaataacc ctgataaatg cttcaataat
     2341 attgaaaaag gaagagtatg agtattcaac atttccgtgt cgcccttatt cccttttttg
     2401 cggcattttg ccttcctgtt tttgctcacc cagaaacgct ggtgaaagta aaagatgctg
     2461 aagatcagtt gggtgcacga gtgggttaca tcgaactgga tctcaacagc ggtaagatcc
     2521 ttgagagttt tcgccccgaa gaacgttttc caatgatgag cacttttaaa gttctgctat
     2581 gtggcgcggt attatcccgt attgacgccg ggcaagagca actcggtcgc cgcatacact
     2641 attctcagaa tgacttggtt gagtactcac cagtcacaga aaagcatctt acggatggca
     2701 tgacagtaag agaattatgc agtgctgcca taaccatgag tgataacact gcggccaact
     2761 tacttctgac aacgatcgga ggaccgaagg agctaaccgc ttttttgcac aacatggggg
     2821 atcatgtaac tcgccttgat cgttgggaac cggagctgaa tgaagccata ccaaacgacg
     2881 agcgtgacac cacgatgcct gtagcaatgg caacaacgtt gcgcaaacta ttaactggcg
     2941 aactacttac tctagcttcc cggcaacaat taatagactg gatggaggcg gataaagttg
     3001 caggaccact tctgcgctcg gcccttccgg ctggctggtt tattgctgat aaatctggag
     3061 ccggtgagcg tgggtctcgc ggtatcattg cagcactggg gccagatggt aagccctccc
     3121 gtatcgtagt tatctacacg acggggagtc aggcaactat ggatgaacga aatagacaga
     3181 tcgctgagat aggtgcctca ctgattaagc attggtaact gtcagaccaa gtttactcat
     3241 atatacttta gattgattta aaacttcatt tttaatttaa aaggatctag gtgaagatcc
     3301 tttttgataa tctcatgacc aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag
     3361 accccgtaga aaagatcaaa ggatcttctt gagatccttt ttttctgcgc gtaatctgct
     3421 gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg tttgccggat caagagctac
     3481 caactctttt tccgaaggta actggcttca gcagagcgca gataccaaat actgtccttc
     3541 tagtgtagcc gtagttaggc caccacttca agaactctgt agcaccgcct acatacctcg
     3601 ctctgctaat cctgttacca gtggctgctg ccagtggcga taagtcgtgt cttaccgggt
     3661 tggactcaag acgatagtta ccggataagg cgcagcggtc gggctgaacg gggggttcgt
     3721 gcacacagcc cagcttggag cgaacgacct acaccgaact gagataccta cagcgtgagc
     3781 tatgagaaag cgccacgctt cccgaaggga gaaaggcgga caggtatccg gtaagcggca
     3841 gggtcggaac aggagagcgc acgagggagc ttccaggggg aaacgcctgg tatctttata
     3901 gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg
     3961 ggcggagcct atggaaaaac gccagcaacg cggccttttt acggttcctg gccttttgct
     4021 ggccttttgc tcacatgttc tttcctgcgt tatcccctga ttctgtggat aaccgtatta
     4081 ccgcctttga gtgagctgat accgctcgcc gcagccgaac gaccgagcgc agcgagtcag
     4141 tgagcgagga agcggaagag cgcccaatac gcaaaccgcc tctccccgcg cgttggccga
     4201 ttcattaatg cagctggcac gacaggtttc ccgactggaa agcgggcagt gagcgcaacg
     4261 caattaatgt gagttagctc actcattagg caccccaggc tttacacttt atgcttccgg
     4321 ctcgtatgtt gtgtggaatt gtgagcggat aacaattt
//
